Review

anti api5 (Proteintech)

Proteintech is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Proteintech anti api5
Anti Api5, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/api/API5+Antibody/pmc11982726-66-6-8
Average 93 stars, based on 6 article reviews

anti api5 - by Bioz Stars, 2026-11

93/100 stars

Images

other:

Article Title: API5 Phosphorylation Promotes Antiviral Immunity by Inhibiting Degradation of Cytosolic RNA Sensor RLRs
Article Snippet: The following antibodies were used in this study: Rabbit anti‐Flag Tag (0912), Rabbit anti‐His Tag (0812), Mouse anti‐GST Tag (EM80701), Mouse anti‐β‐Actin (M1210), Mouse anti‐Histone H3 (M1306), Rabbit anti‐IKKα/β (ET1611‐23), Rabbit anti‐p65 (ET1603‐12), and Rabbit anti‐ATG5 (ET1611‐38) were purchased by HuaAn Biotechnology; Mouse anti‐Flag Tag (F1804) was from Sigma–Aldrich; Rabbit anti‐GAPDH (AB‐P‐R001) was from GoodHere Technology; Mouse anti‐influenza viral proteins were prepared and stored in the laboratory; Rabbit anti‐API5 (ab65836) and Rabbit anti‐p62 (ab109012) were from Abcam; Mouse anti‐API5 (sc‐393341) and Mouse anti‐RIG‐I (sc‐376845) were from Santa Cruz; Rabbit anti‐SRPK1(A5854) and Rabbit anti‐MDA5 (A13645) were from ABclonal; Rabbit anti‐ RIG‐I (67556‐1‐Ig) and Rabbit anti‐IRF3 (11312‐1‐AP) were from Proteintech; Rabbit anti‐phospho‐IRF3 (13 786) was from Signalway Antibody; Rabbit anti‐MDA5 (5321), Mouse anti‐Ubiquitin (3936), Rabbit anti‐TBK1(3504), Rabbit anti‐phospho‐TBK1 (5483), Rabbit anti‐phospho‐IKKα/β (2697), Rabbit anti‐phospho‐p65 (3033), Rabbit anti‐phospho‐IκBα (2859) and Rabbit anti‐LC3B (2775) were purchased by Cell Signaling Technology; HRP‐conjugated goat‐anti mouse IgG (074‐1806) and HRP‐conjugated goat‐anti rabbit IgG (074‐1506) were from Kirkegaard & Perry Laboratories, Inc. Bafilomycin A1(BafA1; HY‐100558), chloroquine (CQ; HY‐17589A), 3‐methyladenine (3‐MA; HY‐19312), MG132 (HY‐13259), Lactacystin (Lacta; HY‐16594), SRPIN340 (HY‐13949,) and cycloheximide (CHX; HY‐12320) were purchased from MedChemExpress.

Article Title: API5 Phosphorylation Promotes Antiviral Immunity by Inhibiting Degradation of Cytosolic RNA Sensor RLRs.
Article Snippet: [14,22] Poly (I:C)-LMW (tlrl-picw), poly (I:C)HMW (tlrl-pic) and puromycin (58-58-2) were provided by Invivogen.

Western Blot:

Article Title: Itaconate targets the ERK2 signal to suppress estrogen receptor-positive breast cancer cell growth
Article Snippet: .. Western blot examined nuclear proteins using anti-API5 (25689-1-AP, Proteintech) for detection, with Lamin A/C (sc-376248, Santa Cruz Biotechnology) as the nuclear marker. .. Cells were lysed in RIPA buffer (R0278, Sigma-Aldrich) to obtain the lysates for subjecting to Western blot analysis using specific primary antibodies.

Marker:

Incubation:

Article Title: Itaconate targets the ERK2 signal to suppress estrogen receptor-positive breast cancer cell growth
Article Snippet: .. Fixed and permeabilized cells were incubated overnight at 4°C with an anti-API5 antibody (25689-1-AP, Proteintech) diluted 1:200. .. Secondary detection was performed with Alexa Fluor 488-conjugated anti-rabbit antibody (A-11008, Thermo Scientific) at a 1:400 dilution for 1 hour at room temperature.