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Cell Signaling Technology Inc test method blend components characteristic resid 1 resid 2 resid 3 resid 4 resid 5 decant oil vgo lco hco api gravity
Test Method Blend Components Characteristic Resid 1 Resid 2 Resid 3 Resid 4 Resid 5 Decant Oil Vgo Lco Hco Api Gravity, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/api+2/CHD4+Rabbit+mAb/us12421467-358-2-54
Average 93 stars, based on 23 article reviews

test method blend components characteristic resid 1 resid 2 resid 3 resid 4 resid 5 decant oil vgo lco hco api gravity - by Bioz Stars, 2026-10

93/100 stars

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Western Blot:

Article Title: Top2a complexes with Trim28 to orchestrate stemness of embryonic stem cells and tumorigenesis
Article Snippet: Protein bands were visualized using an ECL substrate (PerkinElmer, NEL105001EA). .. The following antibodies were used for WB and IP-WB: H3K4me3 (Abcam, ab8580, 1:5000), α-Top2a (Beyotime, AF0303, 1:1000), Top2a (Abcam, ab52934, 1:5000), α-tubulin (CST, 2144S, 1:1000), H3K9me3 (CST, 13969S, 1:1000), H3K9me3 (Abcam, ab8898, 1:5000), H3K27me3 (CST, 9733S, 1:1000), P53 (Santa Cruz, sc-6243, 1:1000), KAP1 (Abcam, ab22553, 1:500), TIF1b (CST, 4123S, 1:1000), Oct-3/4 (Santa Cruz, sc5279, 1:1000), Oct4 (CST, D6C8T, 1:1000), Nanog (Bethyl Laboratories, A300-397A, 1:5000), Anti-KAP1 (Abcam, ab70369, 1:5000), Sox2 (Abcam, ab97959, 1:5000), Anti-Histone H3 Antibody (CT, Pan Millipore, 07-690, 1:50000), HP1α (Abcam, ab77256, 1:5000), H3K9ac (CST, 9649S, 1:1000), CHD3 Antibody (CST, 4241, 1:1000), CHD4 (D8B12) Rabbit (CST, 11912, 1:1000), HDAC1 (10E2)Mouse (CST, 5356, 1;1000), HDAC2 (3F3) Mouse (CST, 5113, 1;1000), MBD3 (N87) Antibody (CST, 14540, 1:1000), MTA1 (D40D1) XP Rabbit (CST, 5647, 1:1000), RBAP46 (V415) Antibody (CST, 6882, 1:1000), Gatad2a (Abcam, ab87663, 1:10000), Gatad2b (Abcam, ab76925, 1:10000), Rbbp7 (CST, 6882T, 1:1000), His-tag ab (Genscript, A00186-100, 1:1000), Anti-KAP1 (phospho S824) antibody (Abcam, ab70369, 1:5000), HP1γ (Santa Cruz, sc398562, 1:1000), H3K27ac (CST, 8173s, 1:1000), Mouse Phospho-p53 (S18) Antibody (R&D, AF2887, 1:1000), JARID1B Antibody(KDM5B) (CST, 3273T, 1:1000), Ezh1 antibody (CST, 42088, 1:1000), Ezh2 antibody (CST, 5246, 1:1000), Suz12 antibody (CST, 3737, 1:1000), Eed antibody (CST, 51673, 1:1000), Jarid2 antibody (CST, 13594, 1;1000), Normal mouse IgG (Santa AR TI CL E IN P RE SS Cruz , sc-2025, 1:1000), Normal goat IgG (Santa Cruz, sc-2028, 1:1000), Normal rabbit IgG (Santa Cruz , sc-2027, 1:1000), Goat anti-rabbit IgG (H+L) secondary antibody (Invitrogen, 65-6120, 1:3000), Goat anti-mouse IgG (H+L) secondary antibody (Invitrogen, 62-6520, 1:3000), donkey anti-goat IgG-HRP (Santa Cruz, sc-2020, 1:3000), Peroxidase AffiniPureTM Goat Anti-Mouse IgG (Jackson Immuno Research, 115-035-174, 1:3000). .. Reverse transcription and quantitative real-time PCR (RT-qPCR) Total RNA was extracted using TRIzol Reagent (Thermo Fisher, 15596018) and cDNA was synthesized with 1 μg of total RNAs with HiScript III RT Supermix (Vazyme, R323-01).

Incubation:

Article Title: The Chromatin Remodeler Protein CHD4 Cooperates With NKX2.2 to Regulate Pancreatic Beta Cell Integrity
Article Snippet: For mass spec of endogenous CHD4 protein, pulldowns were performed in WT MIN6 cells using the Nuclear Complex Co-IP Kit (Active Motif, Cat. #54001) per the manufacturer’s instructions. .. Briefly, 500 μg of WT MIN6 nuclear extract was incubated in IP High Buffer (no detergent and no NaCl) using 5 μg of CHD4 rabbit antibody (D8B12, CST, Cat. #11912) or 5 μg of a control IgG rabbit antibody (CST, Cat. #2729), overnight with mixing. .. The second day, samples were incubated with Anti-Protein-A magnetic beads (Pierce, Cat. #88846) for 1 hour with mixing at 4°C.

Control:

other:

Article Title: HP1 enhances radio- and chemotherapy resistance in osteosarcoma via PARP1-dependent transcriptional repression and HR repair.
Article Snippet: Osteosarcoma is a primary malignant bone tumour with an unsatisfactory prognosis, and individualised and comprehensive treatment is needed.. HP1 (α/β/γ) is an important heterochromatin protein involved in transcriptional regulation and is also involved in the DNA damage response (DDR).. However, little is known about the participation of HP1 in DNA damage-induced transcriptional repression.

Article Title: CHD8 interacts with BCL11A to induce oncogenic transcription in triple negative breast cancer
Article Snippet: Anti-CHD4 antibody , Cell Signalling Technology , 11912.

Residue:

Article Title: Low sulfur fuel oil blends for stability enhancement and associated methods
Article Snippet: .. TABLE VI Test Method Blend Components Characteristic Resid 1 Resid 2 Resid 3 Resid 4 Resid 5 Decant Oil VGO LCO HCO API Gravity @ ~60° F. 5.8 11.9 12.9 14.3 13.9 −0.3 25.3 39.0 39.0 Density @ ~15° C. (g/ml) 0.999 0.987 0.949 0.939 0.960 1.049 0.900 0.830 0.830 Viscocisty @ ~50° C. (cSt) 473.78 355.43 2234.82 888.93 10116.20 189.68 23.35 5.00 35.06 Sulfur (wt %) 1.74 2.51 0.42 1.38 1.59 0.30 0.05 0.05 0.17 Flash Point (° C.) 178.0 99.0 132.0 109.5 159.0 57.5 60.5 Pour Point (° C.) 53.6 35.0 24.0 Potential Total Sediment (wt %) Ash Content (wt %) 10 Vanadium (wppm) 42.8 167.0 16.5 71.8 93.1 0.3 0.2 0.2 0.2 Sodium (wppm) 9.4 16.1 10.8 7.6 1.1 1.0 1.0 1.0 1.0 Aluminum + Silicon (wppm) 27 40 20 1 Copper (wppm) 0.3 0.4 0.3 0.5 0.3 0.3 0.3 0.3 0.3 Calcium (wppm) 4.69 7.64 6.02 2.77 5.74 0.20 0.20 0.20 0.20 Zinc (wppm) 1.24 3.11 0.91 1.02 2.31 0.40 0.40 0.40 Phosphorus (wppm) 1.16 2.53 1.79 1.35 2.45 1.00 1.00 1.00 1.00 Nickel (wppm) 31.7 67.6 16.1 33.3 37.6 0.3 0.3 0.3 0.3 Iron (wppm) 55 31.4 19.1 7.04 20.7 0.40 0.20 0.20 0.21 Micro Carbon Residue (wt %) 17.16 14.25 17.32 15.57 12.3 4.73 0.04 0.27 0.76 Total Acid Number (mg KOH/kg) 0.10 0.76 0.19 0.18 0.32 0.03 0.02 0.01 0.03 Saturates 10.38 15.7 15.05 13.29 28.62 10.05 56.12 11.21 22.09 Aromatics 70.16 50.06 55.13 54.10 47.43 86.45 41.85 88.78 72.08 Resins 10.32 20.88 18.57 22.1 13.09 2.40 0.53 0 1.77 Aspaltenes 9.12 13.3 11.2 10.5 10.9 1.1 0 0 4.1 CII 0.242 0.409 0.357 0.312 0.652 0.125 1.324 0.126 0.354 Heptane Insolubles 6.42 8.78 8.55 2.43 0.29 0.17 TABLE VII Blend Compositions Component Blnd #1 Blnd #2 Blnd #3 Blnd #4 Blnd #5 Blnd #6 Blnd #7 Resid 1 12.02 23.28 Resid 2 12.84 Resid 3 25.50 Resid 4 24.81 23.36 Resid 5 24.59 Decant Oil 30.66 40.32 53.94 36.94 50.23 47.02 13.59 Vacuum Gas Oil 44.53 46.84 37.56 63.12 Light Cycle Oil 34.05 26.42 Heavy Cycle Oil 28.38 Blend Compositions Component Blnd #8 Blnd #9 Blnd #10 Blnd #11 Blnd #12 Blnd #13 Blnd #14 Resid 1 24.71 Resid 2 23.81 Resid 3 26.29 25.50 22.42 Resid 4 25.89 25.51 Resid 5 Decant Oil 42.35 57.12 36.94 16.24 41.76 32.00 13.70 Vacuum Gas Oil 32.95 16.59 37.56 61.33 32.35 42.49 62.49 Light Cycle Oil Heavy Cycle Oil ..

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$Red light inhibits type I collagen degradation via AKT/NRF2/HO-1 signaling pathway activation A, B: qPCR analysis of NRF2 (A) and HO-1 (B) mRNA expression in mouse skin tissue following red light treatment. n =4. C: Representative western blot assays of p-AKT, HO-1, NRF2, and type I collagen expression in aged mice treated with red light, API-2, or both. D: Western blot analysis of nuclear-cytoplasmic separation in skin tissue. Cytosolic and nuclear extracts were immunoblotted for NRF2, while GAPDH and histone H3 proteins were probed to confirm thorough separation of the cytosolic and nuclear fractions. E: Representative western blot analysis of type I collagen expression in skin tissue after red light treatment with or without HO-1i. F: Representative immunofluorescence images of type I collagen in skin sections from aged mice after 28 days of red light treatment with or without HO-1i. Scale bar: 50 μm. G: Heatmaps showing relative expression levels of DEGs involved in various MMPs. n =3. H: qPCR analysis of MMP3, MMP9, MMP12, and MMP13 mRNA expression in mouse skin tissue following red light treatment, with and without HO-1i. *** : P <0.001; **** : P <0.0001 compared to control group, and #### : P <0.0001 compared to indicated groups, one-way ANOVA for multiple groups. Data are mean±SEM.$

Journal: Zoological Research

Article Title: Red light promotes dermis-epidermis remodeling via TGFβ and AKT-mediated collagen dynamics in naturally aging mice

doi: 10.24272/j.issn.2095-8137.2024.405

Figure Lengend Snippet: Red light inhibits type I collagen degradation via AKT/NRF2/HO-1 signaling pathway activation A, B: qPCR analysis of NRF2 (A) and HO-1 (B) mRNA expression in mouse skin tissue following red light treatment. n =4. C: Representative western blot assays of p-AKT, HO-1, NRF2, and type I collagen expression in aged mice treated with red light, API-2, or both. D: Western blot analysis of nuclear-cytoplasmic separation in skin tissue. Cytosolic and nuclear extracts were immunoblotted for NRF2, while GAPDH and histone H3 proteins were probed to confirm thorough separation of the cytosolic and nuclear fractions. E: Representative western blot analysis of type I collagen expression in skin tissue after red light treatment with or without HO-1i. F: Representative immunofluorescence images of type I collagen in skin sections from aged mice after 28 days of red light treatment with or without HO-1i. Scale bar: 50 μm. G: Heatmaps showing relative expression levels of DEGs involved in various MMPs. n =3. H: qPCR analysis of MMP3, MMP9, MMP12, and MMP13 mRNA expression in mouse skin tissue following red light treatment, with and without HO-1i. *** : P <0.001; **** : P <0.0001 compared to control group, and #### : P <0.0001 compared to indicated groups, one-way ANOVA for multiple groups. Data are mean±SEM.

Article Snippet: To inhibit AKT, mice received intraperitoneal (i.p.) injections of API-2 (HY-15457, MCE, USA) at a dose of 2 mg/kg every 48 h. To inhibit TGFβ, mice received i.p. injections of SB431542 (HY-10431, MCE, USA), a TGFβR1 inhibitor, at a dose of 10 mg/kg every 48 h. To inhibit cAMP, mice received i.p. injections of SQ22536 (HY-100396, MCE, USA), an adenylate cyclase (AC) inhibitor, at a dose of 10 mg/kg every 48 h. To inhibit HO-1, mice received i.p. injections of HO-1i (HY-111798A, MCE, USA) at a dose of 1.357 mg/kg every 48 h. To inhibit intracellular free Ca 2+ , mice received i.p. injections of diltiazem (HY-B0632, MCE, USA) at a dose of 20 mg/kg every 48 h. To inhibit reactive oxygen species (ROS), mice received daily topical application of 100 mg/mL ascorbic acid (HY-B0166, MCE, USA), applied to the skin 30 min after red light exposure.

Techniques: Activation Assay, Expressing, Western Blot, Immunofluorescence, Control