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antisense oligonucleotide of 48-mer for the rat th cdna sequence  (Lofstrand)

 
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    Structured Review

    Lofstrand antisense oligonucleotide of 48-mer for the rat th cdna sequence
    Antisense Oligonucleotide Of 48 Mer For The Rat Th Cdna Sequence, supplied by Lofstrand, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antisense+sequences/antisense+oligonucleotide+of+48+mer+for+the+rat+th+cdna+sequence/pm16205776-85-3-13
    Average 90 stars, based on 1 article reviews
    antisense oligonucleotide of 48-mer for the rat th cdna sequence - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: A centrally acting, anxiolytic angiotensin II AT1 receptor antagonist prevents the isolation stress-induced decrease in cortical CRF1 receptor and benzodiazepine binding.
    Article Snippet: .. We synthesized one antisense oligonucleotide of 48-mer for the rat TH cDNA sequence (Lofstrand Labs Limited, MD), localized in nt 1562–1609 (Grima et al, 1985), and labeled the oligonucleotide with terminal deoxynucleotidyl transferase (Amersham) to a specific activity of 3– 4 108 dpm/mg. .. Each reaction was performed with 70 pmol of oligonucleotides in the presence of 70 mCi of [a-35S]ATP (Amersham).

    Article Title: Estrogen Reduces Aldosterone, Upregulates Adrenal Angiotensin II AT 2 Receptors and Normalizes Adrenomedullary Fra-2 in Ovariectomized Rats
    Article Snippet: .. We synthesized an antisense oligonucleotide of 48 bases as a probe for the rat TH cDNA sequence (Lofstrand Labs Ltd, Gaithersburg, Md., USA), localized in nt 1562–1609 [ 32 ]. .. We labeled the oligonucleotide using terminal deoxynucleotidyl transferase (GE Healthcare, Little Chalfont, Bucks., UK) to a specific activity of 3–4 × 10 8 dpm/μg.

    Article Title: Oral administration of an AT1 receptor antagonist prevents the central effects of angiotensin II in spontaneously hypertensive rats.
    Article Snippet: Peripheral and brain angiotensin II AT1 receptor blockade decreases high blood pressure, stress, and neuronal injury.. To clarify the effects of long-term brain Ang II receptor blockade, the AT1 blocker, candesartan, was orally administered to spontaneously hypertensive rats (SHRs) for 40 days, followed by intraventricular injection of 25 ng of Ang II.. Before Ang II injection, AT1 receptor blockade normalized blood pressure and decreased plasma adrenocorticotropic hormone (ACTH) and corticosterone.

    Article Title: A peripherally administered, centrally acting angiotensin II AT 2 antagonist selectively increases brain AT 1 receptors and decreases brain tyrosine hydroxylase transcription, pituitary vasopressin and ACTH
    Article Snippet: .. One antisense (TH-AS) oligonucleotide of 48-mer for the rat TH cDNA sequence ( Grima et al., 1985 ), localized in nt 1562–1609, synthesized by Lofstrand Labs Ltd (Gaithersburg, Md., USA) was labeled with a 3′-end labeling kit (Amersham) using terminal deoxynucleotidyl transferase to a specific activity of 3–4×10 8 dpm/μg. .. Each reaction was performed with 70 pmol of oligonucleotides in the presence of 70 μCi of [ 35 S] ATP (SJ 1334) (Amersham).

    Article Title: Anti-inflammatory effects of angiotensin II AT1 receptor antagonism prevent stress-induced gastric injury.
    Article Snippet: .. One antisense oligonucleotide of 48-mer for the rat TH cDNA sequence was synthesized (Lofstrand Labs). ..

    Article Title: The serotonin transporter is required for stress-evoked increases in adrenal catecholamine synthesis and angiotensin II AT(2) receptor expression.
    Article Snippet: High numbers of serotonin transporter (SERT) binding sites and high serotonin (5-HT) content are expressed in the adrenal medulla of wild-type (SERT+/+) mice.. Acute restraint stress increases adrenomedullary 5-HT, norepinephrine (NE) and epinephrine (E) release, adrenomedullary tyrosine hydroxylase (TH) mRNA, and angiotensin II AT2 receptor expression.. There are no alterations in adrenal catecholamine content during restraint.

    Sequencing:

    Article Title: A centrally acting, anxiolytic angiotensin II AT1 receptor antagonist prevents the isolation stress-induced decrease in cortical CRF1 receptor and benzodiazepine binding.
    Article Snippet: .. We synthesized one antisense oligonucleotide of 48-mer for the rat TH cDNA sequence (Lofstrand Labs Limited, MD), localized in nt 1562–1609 (Grima et al, 1985), and labeled the oligonucleotide with terminal deoxynucleotidyl transferase (Amersham) to a specific activity of 3– 4 108 dpm/mg. .. Each reaction was performed with 70 pmol of oligonucleotides in the presence of 70 mCi of [a-35S]ATP (Amersham).

    Article Title: Estrogen Reduces Aldosterone, Upregulates Adrenal Angiotensin II AT 2 Receptors and Normalizes Adrenomedullary Fra-2 in Ovariectomized Rats
    Article Snippet: .. We synthesized an antisense oligonucleotide of 48 bases as a probe for the rat TH cDNA sequence (Lofstrand Labs Ltd, Gaithersburg, Md., USA), localized in nt 1562–1609 [ 32 ]. .. We labeled the oligonucleotide using terminal deoxynucleotidyl transferase (GE Healthcare, Little Chalfont, Bucks., UK) to a specific activity of 3–4 × 10 8 dpm/μg.

    Article Title: Oral administration of an AT1 receptor antagonist prevents the central effects of angiotensin II in spontaneously hypertensive rats.
    Article Snippet: Peripheral and brain angiotensin II AT1 receptor blockade decreases high blood pressure, stress, and neuronal injury.. To clarify the effects of long-term brain Ang II receptor blockade, the AT1 blocker, candesartan, was orally administered to spontaneously hypertensive rats (SHRs) for 40 days, followed by intraventricular injection of 25 ng of Ang II.. Before Ang II injection, AT1 receptor blockade normalized blood pressure and decreased plasma adrenocorticotropic hormone (ACTH) and corticosterone.

    Article Title: A peripherally administered, centrally acting angiotensin II AT 2 antagonist selectively increases brain AT 1 receptors and decreases brain tyrosine hydroxylase transcription, pituitary vasopressin and ACTH
    Article Snippet: .. One antisense (TH-AS) oligonucleotide of 48-mer for the rat TH cDNA sequence ( Grima et al., 1985 ), localized in nt 1562–1609, synthesized by Lofstrand Labs Ltd (Gaithersburg, Md., USA) was labeled with a 3′-end labeling kit (Amersham) using terminal deoxynucleotidyl transferase to a specific activity of 3–4×10 8 dpm/μg. .. Each reaction was performed with 70 pmol of oligonucleotides in the presence of 70 μCi of [ 35 S] ATP (SJ 1334) (Amersham).

    Article Title: Anti-inflammatory effects of angiotensin II AT1 receptor antagonism prevent stress-induced gastric injury.
    Article Snippet: .. One antisense oligonucleotide of 48-mer for the rat TH cDNA sequence was synthesized (Lofstrand Labs). ..

    Article Title: The serotonin transporter is required for stress-evoked increases in adrenal catecholamine synthesis and angiotensin II AT(2) receptor expression.
    Article Snippet: High numbers of serotonin transporter (SERT) binding sites and high serotonin (5-HT) content are expressed in the adrenal medulla of wild-type (SERT+/+) mice.. Acute restraint stress increases adrenomedullary 5-HT, norepinephrine (NE) and epinephrine (E) release, adrenomedullary tyrosine hydroxylase (TH) mRNA, and angiotensin II AT2 receptor expression.. There are no alterations in adrenal catecholamine content during restraint.

    Labeling:

    Article Title: A centrally acting, anxiolytic angiotensin II AT1 receptor antagonist prevents the isolation stress-induced decrease in cortical CRF1 receptor and benzodiazepine binding.
    Article Snippet: .. We synthesized one antisense oligonucleotide of 48-mer for the rat TH cDNA sequence (Lofstrand Labs Limited, MD), localized in nt 1562–1609 (Grima et al, 1985), and labeled the oligonucleotide with terminal deoxynucleotidyl transferase (Amersham) to a specific activity of 3– 4 108 dpm/mg. .. Each reaction was performed with 70 pmol of oligonucleotides in the presence of 70 mCi of [a-35S]ATP (Amersham).

    Article Title: Oral administration of an AT1 receptor antagonist prevents the central effects of angiotensin II in spontaneously hypertensive rats.
    Article Snippet: Peripheral and brain angiotensin II AT1 receptor blockade decreases high blood pressure, stress, and neuronal injury.. To clarify the effects of long-term brain Ang II receptor blockade, the AT1 blocker, candesartan, was orally administered to spontaneously hypertensive rats (SHRs) for 40 days, followed by intraventricular injection of 25 ng of Ang II.. Before Ang II injection, AT1 receptor blockade normalized blood pressure and decreased plasma adrenocorticotropic hormone (ACTH) and corticosterone.

    Article Title: A peripherally administered, centrally acting angiotensin II AT 2 antagonist selectively increases brain AT 1 receptors and decreases brain tyrosine hydroxylase transcription, pituitary vasopressin and ACTH
    Article Snippet: .. One antisense (TH-AS) oligonucleotide of 48-mer for the rat TH cDNA sequence ( Grima et al., 1985 ), localized in nt 1562–1609, synthesized by Lofstrand Labs Ltd (Gaithersburg, Md., USA) was labeled with a 3′-end labeling kit (Amersham) using terminal deoxynucleotidyl transferase to a specific activity of 3–4×10 8 dpm/μg. .. Each reaction was performed with 70 pmol of oligonucleotides in the presence of 70 μCi of [ 35 S] ATP (SJ 1334) (Amersham).

    Article Title: The serotonin transporter is required for stress-evoked increases in adrenal catecholamine synthesis and angiotensin II AT(2) receptor expression.
    Article Snippet: High numbers of serotonin transporter (SERT) binding sites and high serotonin (5-HT) content are expressed in the adrenal medulla of wild-type (SERT+/+) mice.. Acute restraint stress increases adrenomedullary 5-HT, norepinephrine (NE) and epinephrine (E) release, adrenomedullary tyrosine hydroxylase (TH) mRNA, and angiotensin II AT2 receptor expression.. There are no alterations in adrenal catecholamine content during restraint.

    Activity Assay:

    Article Title: A centrally acting, anxiolytic angiotensin II AT1 receptor antagonist prevents the isolation stress-induced decrease in cortical CRF1 receptor and benzodiazepine binding.
    Article Snippet: .. We synthesized one antisense oligonucleotide of 48-mer for the rat TH cDNA sequence (Lofstrand Labs Limited, MD), localized in nt 1562–1609 (Grima et al, 1985), and labeled the oligonucleotide with terminal deoxynucleotidyl transferase (Amersham) to a specific activity of 3– 4 108 dpm/mg. .. Each reaction was performed with 70 pmol of oligonucleotides in the presence of 70 mCi of [a-35S]ATP (Amersham).

    Article Title: Oral administration of an AT1 receptor antagonist prevents the central effects of angiotensin II in spontaneously hypertensive rats.
    Article Snippet: Peripheral and brain angiotensin II AT1 receptor blockade decreases high blood pressure, stress, and neuronal injury.. To clarify the effects of long-term brain Ang II receptor blockade, the AT1 blocker, candesartan, was orally administered to spontaneously hypertensive rats (SHRs) for 40 days, followed by intraventricular injection of 25 ng of Ang II.. Before Ang II injection, AT1 receptor blockade normalized blood pressure and decreased plasma adrenocorticotropic hormone (ACTH) and corticosterone.

    Article Title: A peripherally administered, centrally acting angiotensin II AT 2 antagonist selectively increases brain AT 1 receptors and decreases brain tyrosine hydroxylase transcription, pituitary vasopressin and ACTH
    Article Snippet: .. One antisense (TH-AS) oligonucleotide of 48-mer for the rat TH cDNA sequence ( Grima et al., 1985 ), localized in nt 1562–1609, synthesized by Lofstrand Labs Ltd (Gaithersburg, Md., USA) was labeled with a 3′-end labeling kit (Amersham) using terminal deoxynucleotidyl transferase to a specific activity of 3–4×10 8 dpm/μg. .. Each reaction was performed with 70 pmol of oligonucleotides in the presence of 70 μCi of [ 35 S] ATP (SJ 1334) (Amersham).

    Article Title: The serotonin transporter is required for stress-evoked increases in adrenal catecholamine synthesis and angiotensin II AT(2) receptor expression.
    Article Snippet: High numbers of serotonin transporter (SERT) binding sites and high serotonin (5-HT) content are expressed in the adrenal medulla of wild-type (SERT+/+) mice.. Acute restraint stress increases adrenomedullary 5-HT, norepinephrine (NE) and epinephrine (E) release, adrenomedullary tyrosine hydroxylase (TH) mRNA, and angiotensin II AT2 receptor expression.. There are no alterations in adrenal catecholamine content during restraint.

    other:

    Article Title: A peripherally administered, centrally acting angiotensin II AT 2 antagonist selectively increases brain AT 1 receptors and decreases brain tyrosine hydroxylase transcription, pituitary vasopressin and ACTH
    Article Snippet: In situ hybridization of tyrosine hydroxylase mRNA One antisense (TH-AS) oligonucleotide of 48-mer for the rat TH cDNA sequence ( Grima et al., 1985 ), localized in nt 1562–1609, synthesized by Lofstrand Labs Ltd (Gaithersburg, Md., USA) was labeled with a 3′-end labeling kit (Amersham) using terminal deoxynucleotidyl transferase to a specific activity of 3–4×10 8 dpm/μg.

    Article Title: Candesartan decreases the sympatho-adrenal and hormonal response to isolation stress.
    Article Snippet: In situ hybridisation of tyrosine hydroxylase mRNA One antisense (TH-AS) of 48-mer for the rat tyrosine hydroxylase (TH) cDNA sequence localised in nt 1562–160925 was synthesised (Lofstrand Labs Limited, MD).



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    tiRNA-Met directly interacted with SNRPA and promoted its ubiquitination. A , B Silver-stained SDS-PAGE gel images showed proteins immunoprecipitated by tiRNA-Met and its <t>antisense</t> RNA in MDA-MB-231 ( A ) and BT-549 ( B ) cell lines. C Western blot analysis of products from RNA pull-down assays using tiRNA-Met sense and antisense confirmed that tiRNA-Met directly associated with SNRPA. D , E RIP followed by RT-qPCR demonstrated specific binding of tiRNA-Met to SNRPA in MDA-MB-231 ( D ) and BT-549 ( E ) cells. F RNA pull-down and western blot assays revealed that tiRNA-Met bound to the RRM2 domain of SNRPA. G The mRNA expression levels of SNRPA remained unchanged in MDA-MB-231 and BT-549 cells transfected with tiRNA-Met mimics or inhibitor. H Overexpression of tiRNA-Met reduced SNRPA protein levels in both cell lines, while inhibition of tiRNA-Met increased SNRPA protein levels. I MDA-MB-231 cells treated with cycloheximide (Chx; 5 μM) and then transfected with tiRNA-Met mimics exhibited a reduced half-life of endogenous SNRPA protein, as shown by western blot; β-actin served as a loading control. J Treatment with MG132 (20 μg/ml) led to a progressive increase in SNRPA protein levels over time; β-actin was used as a reference. K Immunoprecipitation assays indicated that the binding of SNRPA to ubiquitinated proteins was elevated in MDA-MB-231 cells transfected with tiRNA-Met mimics. All data are presented as means ± SD; ns, P > 0.05; * P < 0.05; ** P < 0.01; **** P < 0.0001
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    Image Search Results


    tiRNA-Met directly interacted with SNRPA and promoted its ubiquitination. A , B Silver-stained SDS-PAGE gel images showed proteins immunoprecipitated by tiRNA-Met and its antisense RNA in MDA-MB-231 ( A ) and BT-549 ( B ) cell lines. C Western blot analysis of products from RNA pull-down assays using tiRNA-Met sense and antisense confirmed that tiRNA-Met directly associated with SNRPA. D , E RIP followed by RT-qPCR demonstrated specific binding of tiRNA-Met to SNRPA in MDA-MB-231 ( D ) and BT-549 ( E ) cells. F RNA pull-down and western blot assays revealed that tiRNA-Met bound to the RRM2 domain of SNRPA. G The mRNA expression levels of SNRPA remained unchanged in MDA-MB-231 and BT-549 cells transfected with tiRNA-Met mimics or inhibitor. H Overexpression of tiRNA-Met reduced SNRPA protein levels in both cell lines, while inhibition of tiRNA-Met increased SNRPA protein levels. I MDA-MB-231 cells treated with cycloheximide (Chx; 5 μM) and then transfected with tiRNA-Met mimics exhibited a reduced half-life of endogenous SNRPA protein, as shown by western blot; β-actin served as a loading control. J Treatment with MG132 (20 μg/ml) led to a progressive increase in SNRPA protein levels over time; β-actin was used as a reference. K Immunoprecipitation assays indicated that the binding of SNRPA to ubiquitinated proteins was elevated in MDA-MB-231 cells transfected with tiRNA-Met mimics. All data are presented as means ± SD; ns, P > 0.05; * P < 0.05; ** P < 0.01; **** P < 0.0001

    Journal: Cellular & Molecular Biology Letters

    Article Title: The novel tRNA-derived fragment, tiRNA-Met, inhibits the malignant progression of triple-negative breast cancer by regulating RANBP3L via a targeted interaction with SNRPA

    doi: 10.1186/s11658-025-00738-2

    Figure Lengend Snippet: tiRNA-Met directly interacted with SNRPA and promoted its ubiquitination. A , B Silver-stained SDS-PAGE gel images showed proteins immunoprecipitated by tiRNA-Met and its antisense RNA in MDA-MB-231 ( A ) and BT-549 ( B ) cell lines. C Western blot analysis of products from RNA pull-down assays using tiRNA-Met sense and antisense confirmed that tiRNA-Met directly associated with SNRPA. D , E RIP followed by RT-qPCR demonstrated specific binding of tiRNA-Met to SNRPA in MDA-MB-231 ( D ) and BT-549 ( E ) cells. F RNA pull-down and western blot assays revealed that tiRNA-Met bound to the RRM2 domain of SNRPA. G The mRNA expression levels of SNRPA remained unchanged in MDA-MB-231 and BT-549 cells transfected with tiRNA-Met mimics or inhibitor. H Overexpression of tiRNA-Met reduced SNRPA protein levels in both cell lines, while inhibition of tiRNA-Met increased SNRPA protein levels. I MDA-MB-231 cells treated with cycloheximide (Chx; 5 μM) and then transfected with tiRNA-Met mimics exhibited a reduced half-life of endogenous SNRPA protein, as shown by western blot; β-actin served as a loading control. J Treatment with MG132 (20 μg/ml) led to a progressive increase in SNRPA protein levels over time; β-actin was used as a reference. K Immunoprecipitation assays indicated that the binding of SNRPA to ubiquitinated proteins was elevated in MDA-MB-231 cells transfected with tiRNA-Met mimics. All data are presented as means ± SD; ns, P > 0.05; * P < 0.05; ** P < 0.01; **** P < 0.0001

    Article Snippet: Biotin-labeled tiRNA-Met sense and antisense sequences were obtained from Genescript (Nanjing, China).

    Techniques: Ubiquitin Proteomics, Staining, SDS Page, Immunoprecipitation, Western Blot, Quantitative RT-PCR, Binding Assay, Expressing, Transfection, Over Expression, Inhibition, Control