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immpress horse antirabbit igg hrp polymer  (Vector Laboratories)


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    Vector Laboratories immpress horse antirabbit igg hrp polymer
    Immpress Horse Antirabbit Igg Hrp Polymer, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antirabbit+igg+polymer+detection+kit/ImmPRESS+HRP+Anti-Rabbit+IgG+(Peroxidase)+Polymer+Detection+Kit%2C+made+in+Horse/pm40407141-126-10-16
    Average 96 stars, based on 1293 article reviews
    immpress horse antirabbit igg hrp polymer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Polymer:

    Article Title: Identification of a recessive PCDH15 nonsense variant in purebred goats with vestibular dysfunction
    Article Snippet: .. Additional sections of brain stem and cerebellum were immunostained for glial fibrillary acid protein (rabbit anti-GFAP, Z0334, DAKO, Glostrup, Denmark), KCNIP4 (rabbit anti-KCNIP4, ab203831, ABCAM, Cambridge, UK), calbindin (rabbit anti-calbindin- d -28k, C 2724, Sigma, Saint Louis, USA), and ITPR1 (rabbit anti IP3 receptor 1, GTX82887, Genetex Inc., Irvine, USA) using an ImmPRESS® polymer technology (HRP horse anti-rabbit IgG polymer detection kit, Vector Laboratories, Newark, USA) and a diaminobenzidine tetrahydrochloride (DAB, Merck, Darmstadt, Germany) detection system. ..

    Article Title: TGF-β/SMAD4/14-3-3σ/TFEB axis promotes mesenchymal-epithelial transition and inhibits autophagy in colorectal cancer
    Article Snippet: Antigen retrieval was performed by microwaving the slides in Target Retrieval Solution Citrate (pH 6.0, Dako) at 750 W for 25 min. After cooling to room temperature, endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 10 min. .. Blocking was then carried out following the protocol of the ImmPRESS HRP Horse Anti-Rabbit (or Anti-Mouse) IgG Polymer Kit (Vector Laboratories). .. Primary antibodies were diluted in Dako Antibody Diluent with Background Reducing Components, applied to slides and incubated overnight at 4 °C.

    Article Title: Iron overload in steatotic hepatocytes drives systemic metabolic dysfunction via alterations in hepatokine production
    Article Snippet: 689 690 Immunohistochemistry and immunofluorescence staining 691 31 For immunohistochemistry (IHC), antigen-retrieval was performed on deparaffinized sections using 692 a citrate buffer solution (#S1699, Dako, Agilent Technologies, Glostrup, Denmark). .. The sections were 693 then stained with specific primary antibodies against FPN (#MTP11-A, Alpha Diagnostic Intl., San 694 Antonio, TX, USA) or UCP1 (#U6382, Sigma-Aldrich), followed by incubation with a horseradish 695 peroxidase (HRP)-conjugated horse anti-rabbit or anti-mouse IgG polymer detection kit (#MP-7401 696 or #MP-7402, Vector Laboratories, Newark, CA, USA). .. Visualization was carried out using a 3,3’-697 diaminobenzidine (DAB) substrate kit (#SK-4100, Vector Laboratories).

    Article Title: Cell fate specification during respiratory development requires ARID1A-containing canonical BAF complex activity.
    Article Snippet: Following secondary antibody application, the sections were quenched to remove autofluorescence using Vector TrueVIEW Autofluorescence Quenching kit (Vector Labs, SP8400), stained with DAPI (Invitrogen, D1306; 1:2000) and mounted using Prolong Gold antifade mounting medium (Invitrogen, P36930). .. Immunofluorescence for Yap (Rabbit, Cell Signaling Technology, 4912 - 1:100), Taz (Rabbit, Cell Signaling Technology, 83669 - 1:200), p-Smad1/5/9 (Rabbit, Cell Signaling Technology, 13820, 1:100) and non-phospho (Active) beta-catenin (Rabbit, Cell Signaling Technology, 8814 - 1:500) was performed using ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Labs, MP-7401-50) which was followed Tyramide signal amplification (TSA Plus Cyanine 3.5, Akoya Biosciences, NEL763001KT) with an 8 min exposure time. ..

    Blocking Assay:

    Article Title: TGF-β/SMAD4/14-3-3σ/TFEB axis promotes mesenchymal-epithelial transition and inhibits autophagy in colorectal cancer
    Article Snippet: Antigen retrieval was performed by microwaving the slides in Target Retrieval Solution Citrate (pH 6.0, Dako) at 750 W for 25 min. After cooling to room temperature, endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 10 min. .. Blocking was then carried out following the protocol of the ImmPRESS HRP Horse Anti-Rabbit (or Anti-Mouse) IgG Polymer Kit (Vector Laboratories). .. Primary antibodies were diluted in Dako Antibody Diluent with Background Reducing Components, applied to slides and incubated overnight at 4 °C.

    Staining:

    Article Title: Iron overload in steatotic hepatocytes drives systemic metabolic dysfunction via alterations in hepatokine production
    Article Snippet: 689 690 Immunohistochemistry and immunofluorescence staining 691 31 For immunohistochemistry (IHC), antigen-retrieval was performed on deparaffinized sections using 692 a citrate buffer solution (#S1699, Dako, Agilent Technologies, Glostrup, Denmark). .. The sections were 693 then stained with specific primary antibodies against FPN (#MTP11-A, Alpha Diagnostic Intl., San 694 Antonio, TX, USA) or UCP1 (#U6382, Sigma-Aldrich), followed by incubation with a horseradish 695 peroxidase (HRP)-conjugated horse anti-rabbit or anti-mouse IgG polymer detection kit (#MP-7401 696 or #MP-7402, Vector Laboratories, Newark, CA, USA). .. Visualization was carried out using a 3,3’-697 diaminobenzidine (DAB) substrate kit (#SK-4100, Vector Laboratories).

    Incubation:

    Article Title: Iron overload in steatotic hepatocytes drives systemic metabolic dysfunction via alterations in hepatokine production
    Article Snippet: 689 690 Immunohistochemistry and immunofluorescence staining 691 31 For immunohistochemistry (IHC), antigen-retrieval was performed on deparaffinized sections using 692 a citrate buffer solution (#S1699, Dako, Agilent Technologies, Glostrup, Denmark). .. The sections were 693 then stained with specific primary antibodies against FPN (#MTP11-A, Alpha Diagnostic Intl., San 694 Antonio, TX, USA) or UCP1 (#U6382, Sigma-Aldrich), followed by incubation with a horseradish 695 peroxidase (HRP)-conjugated horse anti-rabbit or anti-mouse IgG polymer detection kit (#MP-7401 696 or #MP-7402, Vector Laboratories, Newark, CA, USA). .. Visualization was carried out using a 3,3’-697 diaminobenzidine (DAB) substrate kit (#SK-4100, Vector Laboratories).

    Article Title: Identification of gradual aging and late-onset aging markers using male African turquoise killifish.
    Article Snippet: Paraffin sections were de-paraffinized, rehydrated, incubated with 0.3% H2O2/MeOH for 30 min, and blocked in 5% normal horse serum (Thermo fisher scientific, 26050070) in TBS for 1 h. Sections were incubated with anti-nitrotyrosine antibody (Thermo fisher scientific, A21285, 1:200) overnight at 4°C. .. Following washes, sections were incubated with horse anti rabbit IgG (Vector laboratories, MP-7401) for 30 min at room temperature. .. Signals were developed using DAB reagent (Vector laboratories, SK-4105).

    Immunofluorescence:

    Article Title: Cell fate specification during respiratory development requires ARID1A-containing canonical BAF complex activity.
    Article Snippet: Following secondary antibody application, the sections were quenched to remove autofluorescence using Vector TrueVIEW Autofluorescence Quenching kit (Vector Labs, SP8400), stained with DAPI (Invitrogen, D1306; 1:2000) and mounted using Prolong Gold antifade mounting medium (Invitrogen, P36930). .. Immunofluorescence for Yap (Rabbit, Cell Signaling Technology, 4912 - 1:100), Taz (Rabbit, Cell Signaling Technology, 83669 - 1:200), p-Smad1/5/9 (Rabbit, Cell Signaling Technology, 13820, 1:100) and non-phospho (Active) beta-catenin (Rabbit, Cell Signaling Technology, 8814 - 1:500) was performed using ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Labs, MP-7401-50) which was followed Tyramide signal amplification (TSA Plus Cyanine 3.5, Akoya Biosciences, NEL763001KT) with an 8 min exposure time. ..

    Amplification:

    Article Title: Cell fate specification during respiratory development requires ARID1A-containing canonical BAF complex activity.
    Article Snippet: Following secondary antibody application, the sections were quenched to remove autofluorescence using Vector TrueVIEW Autofluorescence Quenching kit (Vector Labs, SP8400), stained with DAPI (Invitrogen, D1306; 1:2000) and mounted using Prolong Gold antifade mounting medium (Invitrogen, P36930). .. Immunofluorescence for Yap (Rabbit, Cell Signaling Technology, 4912 - 1:100), Taz (Rabbit, Cell Signaling Technology, 83669 - 1:200), p-Smad1/5/9 (Rabbit, Cell Signaling Technology, 13820, 1:100) and non-phospho (Active) beta-catenin (Rabbit, Cell Signaling Technology, 8814 - 1:500) was performed using ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Labs, MP-7401-50) which was followed Tyramide signal amplification (TSA Plus Cyanine 3.5, Akoya Biosciences, NEL763001KT) with an 8 min exposure time. ..

    Saline:

    Article Title: Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis
    Article Snippet: .. Sections were then washed thoroughly in Tris-buffered saline (TBS) before applying horse radish peroxidase-conjugated ImmPRESS anti-mouse IgG (Vector Laboratories, MP-7402) or anti-rabbit IgG (Vector Laboratories, MP-7401) secondary antibodies for 30 min at RT. .. Antibody detection was visualised by incubating the sections for 10 min at RT with 3, 3′-diaminobenzidine (DAB; ImmPACT Vector Laboratories, SK-4105).



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