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biotinylated goat anti human lambda  (SouthernBiotech)


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    Structured Review

    SouthernBiotech biotinylated goat anti human lambda
    Biotinylated Goat Anti Human Lambda, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+lambda/Goat+Anti-Human+Lambda-BIOT/us08350011-1367-14-18
    Average 91 stars, based on 7 article reviews
    biotinylated goat anti human lambda - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: MultiSero: An Open-Source Multiplex-ELISA Platform for Measuring Antibody Responses to Infection.
    Article Snippet: .. An amount of 100 μL of serum diluted in blocking buffer was added to each well and allowed to incubate for 1 h. Diluted serum was then aspirated, and the wells were incubated for 1 h with 100 μL of blocking buffer containing 25 ng/mL each of biotinylated goat antibodies targeting the kappa (Southern Biotech, Birmingham, AL, USA cat. 2070-08) and lambda (Southern Biotech cat. 2060-08) light chains, which allows for measurement of total immunoglobulin without preference to isotype. .. Wells were aspirated and incubated for one hour with 100 μL streptavidin conjugated HRP (0.2 μg/mL), aspirated and developed with 50 μL sciCOLOR T2 precipitating TMB reagent (Scienion) for 10 min, and aspirated and imaged at a single time point on a Nautilus or a sciREADER CL2 plate reader.

    Article Title: MultiSero: An Open-Source Multiplex-ELISA Platform for Measuring Antibody Responses to Infection
    Article Snippet: .. An amount of 100 μL of serum diluted in blocking buffer was added to each well and allowed to incubate for 1 h. Diluted serum was then aspirated, and the wells were incubated for 1 h with 100 μL of blocking buffer containing 25 ng/mL each of biotinylated goat antibodies targeting the kappa (Southern Biotech, Birmingham, AL, USA cat. 2070-08) and lambda (Southern Biotech cat. 2060-08) light chains, which allows for measurement of total immunoglobulin without preference to isotype. .. Wells were aspirated and incubated for one hour with 100 μL streptavidin conjugated HRP (0.2 μg/mL), aspirated and developed with 50 μL sciCOLOR T2 precipitating TMB reagent (Scienion) for 10 min, and aspirated and imaged at a single time point on a Nautilus or a sciREADER CL2 plate reader.

    Incubation:

    Article Title: MultiSero: An Open-Source Multiplex-ELISA Platform for Measuring Antibody Responses to Infection.
    Article Snippet: .. An amount of 100 μL of serum diluted in blocking buffer was added to each well and allowed to incubate for 1 h. Diluted serum was then aspirated, and the wells were incubated for 1 h with 100 μL of blocking buffer containing 25 ng/mL each of biotinylated goat antibodies targeting the kappa (Southern Biotech, Birmingham, AL, USA cat. 2070-08) and lambda (Southern Biotech cat. 2060-08) light chains, which allows for measurement of total immunoglobulin without preference to isotype. .. Wells were aspirated and incubated for one hour with 100 μL streptavidin conjugated HRP (0.2 μg/mL), aspirated and developed with 50 μL sciCOLOR T2 precipitating TMB reagent (Scienion) for 10 min, and aspirated and imaged at a single time point on a Nautilus or a sciREADER CL2 plate reader.

    Article Title: MultiSero: An Open-Source Multiplex-ELISA Platform for Measuring Antibody Responses to Infection
    Article Snippet: .. An amount of 100 μL of serum diluted in blocking buffer was added to each well and allowed to incubate for 1 h. Diluted serum was then aspirated, and the wells were incubated for 1 h with 100 μL of blocking buffer containing 25 ng/mL each of biotinylated goat antibodies targeting the kappa (Southern Biotech, Birmingham, AL, USA cat. 2070-08) and lambda (Southern Biotech cat. 2060-08) light chains, which allows for measurement of total immunoglobulin without preference to isotype. .. Wells were aspirated and incubated for one hour with 100 μL streptavidin conjugated HRP (0.2 μg/mL), aspirated and developed with 50 μL sciCOLOR T2 precipitating TMB reagent (Scienion) for 10 min, and aspirated and imaged at a single time point on a Nautilus or a sciREADER CL2 plate reader.

    Article Title: multiSero: open multiplex-ELISA platform for analyzing antibody responses to SARS-CoV-2 infection
    Article Snippet: .. Sample was aspirated and wells incubated for 1 hour with 100 uL of an even mixture of biotinylated goat antibodies targeting the kappa (25 ng mL −1 , Southern Biotech cat. 2070–08) and lambda (25 ng mL −1 Southern Biotech cat. 2060–08) light chains. ..

    Article Title: multiSero: open multiplex-ELISA platform for analyzing antibody responses to SARS-CoV-2 infection
    Article Snippet: .. Sample was aspirated and wells incubated for 1 hour with 100 uL of an even mixture of biotinylated goat antibodies targeting the kappa (25 ng mL −1 , Southern Biotech cat. 2070-08) and lambda (25 ng mL −1 Southern Biotech cat. 2060-08) light chains. ..

    Article Title: Antibodies directed to ERBB2
    Article Snippet: .. Biotinylated goat anti-human Kappa (Vector catalog #BA3060) at 250 ng/ml in 1% milk/PBS or biotinylated goat anti-human Lambda (Southern Biotech catalog #2070-08) at 250 ng/ml in 1% milk/PBS were then added and incubated for 1 hour at room temperature followed by incubation with Streptavidin Peroxidase conjugate at 1 μg/ml in 1% milk/PBS for 1 hour at room temperature. ..

    FACS:

    Article Title: Mechanisms of sterilizing immunity provided by an HIV-1 neutralizing antibody against mucosal infection
    Article Snippet: .. Subsequent to three washes with FACS buffer, the cells were stained with goat polyclonal anti-human Lambda light chain antibodies coupled to AlexaFluor647 (SouthernBiotech, Birmingham, AL, USA) for 20 min at RT in the dark. .. Following three additional washes with FACS buffer, the cells were fixed with 2% PFA for 20 min at RT in the dark and acquired onto an Attune-NxT Flow Cytometer (Thermo Fisher Scientific, Waltham, MA, USA).

    Staining:

    Article Title: Mechanisms of sterilizing immunity provided by an HIV-1 neutralizing antibody against mucosal infection
    Article Snippet: .. Subsequent to three washes with FACS buffer, the cells were stained with goat polyclonal anti-human Lambda light chain antibodies coupled to AlexaFluor647 (SouthernBiotech, Birmingham, AL, USA) for 20 min at RT in the dark. .. Following three additional washes with FACS buffer, the cells were fixed with 2% PFA for 20 min at RT in the dark and acquired onto an Attune-NxT Flow Cytometer (Thermo Fisher Scientific, Waltham, MA, USA).

    Enzyme-linked Immunospot:

    Article Title: Mapping the Lineage Relationship between CXCR5 + and CXCR5 − CD4 + T Cells in HIV-Infected Human Lymph Nodes
    Article Snippet: Polyclonal goat antiserum (2:1000, #38 NIH AIDS Reagent Program) was used as positive control for HIV-specificity and detected using anti-Goat Igg-HRP (1:1000, Santa Cruz Biotech). .. For ELISPOT, 96-well MultiScreenHTS IP Filter plates (Millipore) were coated with goat anti-human kappa/lambda (Southern Biotech), Gag/Env peptide pool (#12425 and #12540, NIH AIDS Reagent Program), or HIV-1 recombinant proteins (#7749, #11784, #12028, #13126, #12577, NIH AIDS Reagent Program) at the final concentration of 5ug/mL in PBS and blocked using RPMI 1640 containing 10% fetal calf serum (FCS). ..

    Recombinant:

    Article Title: Mapping the Lineage Relationship between CXCR5 + and CXCR5 − CD4 + T Cells in HIV-Infected Human Lymph Nodes
    Article Snippet: Polyclonal goat antiserum (2:1000, #38 NIH AIDS Reagent Program) was used as positive control for HIV-specificity and detected using anti-Goat Igg-HRP (1:1000, Santa Cruz Biotech). .. For ELISPOT, 96-well MultiScreenHTS IP Filter plates (Millipore) were coated with goat anti-human kappa/lambda (Southern Biotech), Gag/Env peptide pool (#12425 and #12540, NIH AIDS Reagent Program), or HIV-1 recombinant proteins (#7749, #11784, #12028, #13126, #12577, NIH AIDS Reagent Program) at the final concentration of 5ug/mL in PBS and blocked using RPMI 1640 containing 10% fetal calf serum (FCS). ..

    Concentration Assay:

    Article Title: Mapping the Lineage Relationship between CXCR5 + and CXCR5 − CD4 + T Cells in HIV-Infected Human Lymph Nodes
    Article Snippet: Polyclonal goat antiserum (2:1000, #38 NIH AIDS Reagent Program) was used as positive control for HIV-specificity and detected using anti-Goat Igg-HRP (1:1000, Santa Cruz Biotech). .. For ELISPOT, 96-well MultiScreenHTS IP Filter plates (Millipore) were coated with goat anti-human kappa/lambda (Southern Biotech), Gag/Env peptide pool (#12425 and #12540, NIH AIDS Reagent Program), or HIV-1 recombinant proteins (#7749, #11784, #12028, #13126, #12577, NIH AIDS Reagent Program) at the final concentration of 5ug/mL in PBS and blocked using RPMI 1640 containing 10% fetal calf serum (FCS). ..



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