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Bio-Rad tc20 tm data analyzer software

(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.

Tc20 Tm Data Analyzer Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammation"

Article Title: Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammation

Journal: Cell reports

doi: 10.1016/j.celrep.2025.116758

Figure Legend Snippet: (A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad TC20). Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.

Techniques Used: Flow Cytometry, Cell Characterization, Expressing, MANN-WHITNEY

Histone Association Assay:

Article Title: Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER AC5-LgBit/pEF4 in pEF4 This paper N/A AC6-LgBit/pEF4 in pEF4 This paper N/A AC7-LgBit/pEF4 in pF4A This paper N/A AC9-LgBit/pEF4 in pF4A This paper N/A LgBit-AC2/pEF4 in pEF4 This paper N/A LgBit-AC4/pEF4 in pEF4 This paper N/A LgBit-AC5/pEF4 in pEF4 This paper N/A LgBit-AC6/pEF4 in pEF4 This paper N/A LgBit-AC7/pEF4 in pEF4 This paper N/A LgBit-AC9/pEF4 in pEF4 This paper N/A SmBiT-GNG2(C68S) in pCAGGS Inoue Lab N/A G protein alpha s long (GNAS) cDNA resource center #GNA0SL0000 GNB1 (Gβ1) in pcDNA3.1 Inoue Lab N/A Homo sapiens protein kinase cAMPactivated catalytic subunit beta (PRKACB), var 1 GenScript OHu02335 Homo sapiens protein kinase cAMPactivated catalytic subunit gamma (PRKACG) GenScript OHu31969 Homo sapiens protein kinase cAMPdependent type I regulatory subunit alpha (PRKAR1A), var 1 GenScript OHu16906 Homo sapiens protein kinase cAMPdependent type I regulatory subunit beta (PRKAR1B), var 1 GenScript OHu01423 Homo sapiens protein kinase cAMPdependent type II regulatory subunit beta (PRKAR2B) GenScript OHu03971 SmBiT-PRKACA Control Vector Promega N2015 SmBiT-PRKACB in pFN35K Flexi Vector This paper N/A SmBiT-PRKACG n pFN35K Flexi Vector This paper N/A LgBiT-PRKAR1A in pFN33K Flexi Vector This paper N/A LgBiT-PRKAR1B in pFN33K Flexi Vector This paper N/A LgBiT-PRKAR2A Control Vector Promega N2015 LgBiT-PRKAR2B in pFN33K Flexi Vector This paper N/A beta Arrestin 1 (ARRB1) (NM_004041) Human Tagged ORF Clone ORIGENE RC201279 Arrestin, beta 1 (ARRB1), transcript variant 2 cDNA resource center #ARRB100002 Arrestin, beta 2 (ARRB2), transcript variant 1 cDNA resource center #ARRB200001 Cysltr2-LgBiT in pFC34K Flexi Vector This paper N/A SmBiT-ARRB1 variant1 in pFN35K Vector This paper N/A SmBiT-ARRB1 variant2 in pFN35K Vector This paper N/A SmBiT-ARRB2 variant1 in pFN35K Vector This paper N/A GRK2 in CAGGS Inoue Lab N/A GRK3 in CAGGS Inoue Lab N/A GRK5 in CAGGS Inoue Lab N/A GRK6 in CAGGS Inoue Lab N/A masGRK3ct_(643–688) in pEF4 This paper N/A (Continued on next page) Cell Reports 45, 116758, January 27, 2026 33 .. REAGENT or RESOURCE SOURCE IDENTIFIER KCTD17 (NM_024681) HUman Tagged ORF Colne ORIGENE CAT#RC206070 KCTD17ct in pEF4 This paper N/A Plasmids for NanoBiT association assay (Gβγ/GRKs, Gq/PLCβ1-4) Inoue Lab N/A Plasmids for NanoBiT G protein dissociation assay (Gs, G12, and Gi) Inoue Lab N/A Predesigned shRNA, Human, PRKACA SIGMA TRCN0000233525 Predesigned shRNA, Human, PRKACB SIGMA TRCN0000002002 MISSION® pLKO.1-puro Non- Mammalian shRNA Control Plasmid DNA SIGMA SHC002 MISSION® TRC2 pLKO.5-puro NonMammalian shRNA Control Plasmid DNA SIGMA SHC202 Software and algorithms FlowJo v10.9.0 flow cytometry data analysis BD Biosciences https://www.flowjo.com Prism 9 statistical analysis GraphPad https://www.graphpad.com/features ID7000 Spectral Cell Analyzer SONY https://www.sonybiotechnology.com/ instruments/id7000-spectral-cell-analyzer TC20TM Data Analyzer Software BioRad https://www.bio-rad.com/webroot/web/ html/lsr/products/cell_counting/product_ overlay/global/tc20-data-ana.html AriaMx software Agilent https://www.agilent.com/en/product/real- time-pcr-%28qpcr%29/real-time-pcr%28qpcr%29-instruments/ariamxsoftware-download ImageJ software NIH https://imagej.net/ij/ Prism 9 statistical analysis GraphPad https://www.graphpad.com/features ID7000 Spectral Cell Analyzer SONY https://www.sonybiotechnology.com/ instruments/id7000-spectral-cell-analyzer TC20TM Data Analyzer Software BioRad https://www.bio-rad.com/webroot/web/ html/lsr/products/cell_counting/product_ overlay/global/tc20-data-ana.html AriaMx software Agilent https://www.agilent.com/en/product/real- time-pcr-%28qpcr%29/real-time-pcr%28qpcr%29-instruments/ariamxsoftware-download ImageJ software NIH https://imagej.net/ij/ Other CELL CULTURE MICROPLATE, 384 WELL, μCLEAR Greiner bio-one Cat#781090 Cell strainer Fisher Scientific 22363548 Clear Polystyrene Microplates R&D DY990 384-Well x 120uL Assay Collection And Storage Microplate Nunc Cat#264573 AriaMx 96 Well Plates, Skirted, LP Agilent 401490 AriaMx Adhesive Seals Agilent 401492 Corning® 96-well Solid White Flat Bottom Polystyrene TC-treated Microplates Corning 3917 GloMax Explorer plate reader Promega GM3500 ID7000TM Spectral Flow Cytometer SONY ID7000 BD LSRFortessa BD Biosciences N/A (Continued on next page) 34 Cell Reports 45, 116758, January 27, 2026 .. Cysltr2fl/fl mice were generated by Ingenious targeting laboratory (Ronkonkoma, NY, USA, Figure S5).

shRNA:

Control:

Plasmid Preparation:

Software:

Article Title: A feedback loop between plakophilin 4 and YAP signaling regulates keratinocyte differentiation
Article Snippet: .. The cell diameter was automatically measured using the BioRad TC20 Cell Counter (Bio-Rad Laboratories, Feldkirchen, Germany) and analyzed using the corresponding software (TC20 data analyzer, BioRad). .. For analysis of intercellular cohesion in combination with knockdown, WT and PKP4-KO keratinocytes transfected with non-targeting (Ctrl), YAP-, or TAZ-directed siRNA-pools, were switched to HCM at 48h after transfection and kept in HCM for another 24h before incubation with 2.4 U/ml dispase II (Roche Diagnostics, Indianapolis, IN, USA) in DMEM/Ham’s F12 medium supplemented with 1.2 mM Ca 2+ and 25 mM HEPES for 30min at 37°C.

Article Title: Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline
Article Snippet: Cell Counting Slides for TC10TM/TC20TM Cell Counter, Dual-Chamber, 30 slides, 60 counts , Bio-Rad , Cat#1450011. .. TC20TM Data Analyzer Software , Bio-Rad , Cat#SOFT-TC20-Data-Analyzer. .. Blood Collection tube, BD Vacutainer® K2EDTA 10mL , BD , Cat# BDAM367525.

Article Title: Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammation
Article Snippet: ID7000 Spectral Cell Analyzer , SONY , https://www.sonybiotechnology.com/instruments/id7000-spectral-cell-analyzer. .. TC20 TM Data Analyzer Software , BioRad , https://www.bio-rad.com/webroot/web/html/lsr/products/cell_counting/product_overlay/global/tc20-data-ana.html. .. AriaMx software , Agilent , https://www.agilent.com/en/product/real-time-pcr-%28qpcr%29/real-time-pcr-%28qpcr%29-instruments/ariamx-software-download.

Flow Cytometry:

Cell Culture:

Adhesive:

other:

Article Title: Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline.
Article Snippet: 500 mL Thermo Fisher Scientific Cat#61870-010 eBioscience Protein Transport Inhibitor Cocktail (5003) Thermo Fisher Scientific Cat#00-4980-93 Proteomic Stabilizer PROT1 Smart Tube Inc. Cat#PROT1-1L Antibody Stabilizer PBS CANDOR Bioscience Cat#131 125 Sodium azide, ReagentPlus , R99.5% Sigma-Aldrich Cat#S2002-5G Bond-Breaker TCEP Solution, Neutral pH Thermo Fisher Scientific Cat#77720 Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Scientific Cat#28908 Thaw-Lyse buffer Smart Tube Inc. Cat#PROT1-1L Heparin Sigma-Aldrich Cat#H3393-10KU Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D8418-100ml Dulbecco0s Phosphate Buffered Saline (DPBS) Fisher Scientific S.L Cat#14190-094 Critical commercial assays Maxpar X8 Antibody Labeling Kit, 154Sm—4 Rxn Standard BioTools Cat#201154A Maxpar X8 Antibody Labeling Kit, 160Gd—4 Rxn Standard BioTools Cat#201160A Maxpar X8 Antibody Labeling Kit, 170Er—4 Rxn Standard BioTools Cat#201170A Maxpar X8 Antibody Labeling Kit, 175Lu—4 Rxn Standard BioTools Cat#201175A Deposited data FlowRepository: FR-FCM-Z3YR (Rybakowska et al., 2021a, 2021b) www.flowrepository.org (Continued on next page) ll OPEN ACCESS 14 STAR Protocols 3, 101697, December 16, 2022 Protocol Continued REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms CyTOF Software Version 6.7.1014 Standard BioTools https://www.fluidigm.com/software R software (R Core Team, 2013) http://www.R-project.org/ RStudio RStudio Team https://rstudio.com/ CytoQP This paper, https://doi.org/ 10.5281/zenodo.6947414 https://github.com/prybakowska/ CytoQP BiocParallel (Morgan et al., 2022b) https://bioconductor.org/packages/ release/bioc/html/BiocParallel.html WinRAR N/A https://www.win-rar.com/start.html?&L=12 Other Amicon Ultra-0.5 Centrifugal Filter Unit, 3 kDa Millipore Cat#UFC500396 Amicon Ultra-0.5 Centrifugal Filter Unit, 50 kDa Millipore Cat#UFC505096 MACS SmartStrainers (100 mm) Miltenyi Biotec Cat#130-110-917 TC20 Automated Cell Counter Bio-Rad Cat#1450102 Cell Counting Slides for TC10 /TC20 Cell Counter, Dual-Chamber, 30 slides, 60 counts Bio-Rad Cat#1450011 TC20 Data Analyzer Software Bio-Rad Cat#SOFT-TC20-Data-Analyzer Blood Collection tube, BD Vacutainer K2EDTA 10mL BD Cat# BDAM367525 Axygen 1.7 mL MaxyClear Snaplock Microcentrifuge Tube, Clear, Nonsterile, 500 Tubes/Pack, 10 Packs/Case Axygen Cat# MCT-175-C Eppendorf Safe-Lock Tubes, 0,5 mL, Eppendorf Quality , incoloro, 500 tubos.

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