Micro-Glass Rods:Article Title: Bispecific antibodies that engage pIgR enrich in mucosa in cynomolgus monkeys.
Article Snippet: All recombinant antibodies, as described in Figure 5, were expressed transiently in ExpiCHO-S cells (Thermo Fisher Scientific) according to the manufacturer’s protocols. .. Culture supernatants were harvested and filtered, and titers were measured by surface biolayer interferometry using protein A-based biosensor tips (Octet, Sartorius) according to the manufacturer’s protocols and using a wild-type IgG1 antibody (antiRSV clone B21M) as a control.26 Antibodies were affinity-purified using MabSelect SuRe resin (Cytiva) according to the manufacturer’s protocol. .. Analytical SEC was performed using a G3000SWxl column (Tosoh Bioscience) in phosphate-buffered saline.
Control:Article Title: Bispecific antibodies that engage pIgR enrich in mucosa in cynomolgus monkeys.
Article Snippet: All recombinant antibodies, as described in Figure 5, were expressed transiently in ExpiCHO-S cells (Thermo Fisher Scientific) according to the manufacturer’s protocols. .. Culture supernatants were harvested and filtered, and titers were measured by surface biolayer interferometry using protein A-based biosensor tips (Octet, Sartorius) according to the manufacturer’s protocols and using a wild-type IgG1 antibody (antiRSV clone B21M) as a control.26 Antibodies were affinity-purified using MabSelect SuRe resin (Cytiva) according to the manufacturer’s protocol. .. Analytical SEC was performed using a G3000SWxl column (Tosoh Bioscience) in phosphate-buffered saline.
Article Title: Microbial aldehyde-alcohol dehydrogenase degrades metformin to limit therapeutic response in type 2 diabetes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 μm, 100 Å) Waters Cat# 186003539 Nexera LC-40D x3 UHPLC system Shimadzu LC-40D x3 Triple Quad 5500+ LC-MS/MS System – QTRAP Ready SCIEX Triple Quad 5500+ QTRAP ACQUITY UPLC I-Class PLUS System Waters I-Class PLUS Orbitrap Exploris 120 mass spectrometer Thermo Fisher Scientific Cat# BRE725531; HRAM-MS Monolith NT.115 NanoTemper Technologies Microscale thermophoresis instrument Octet K2 Sartorius / ForteBio Bio-layer interferometry instrument ll OPEN ACCESSArticle Cell Metabolism 38, 1–18.e1–e15, November 3, 2026 e3 Colonization with candidate taxa To test the impact of metformin-modifying species, HFD-fed SPF males received a single gavage of Eubacterium hallii MD1, E. eligens, E. ramulus, E. ventriosum or E. coprostanoligenes (2 × 108 CFU in 0.2 ml sterile anaerobic PBS) or vehicle control. ..
Affinity Purification:Article Title: Bispecific antibodies that engage pIgR enrich in mucosa in cynomolgus monkeys.
Article Snippet: All recombinant antibodies, as described in Figure 5, were expressed transiently in ExpiCHO-S cells (Thermo Fisher Scientific) according to the manufacturer’s protocols. .. Culture supernatants were harvested and filtered, and titers were measured by surface biolayer interferometry using protein A-based biosensor tips (Octet, Sartorius) according to the manufacturer’s protocols and using a wild-type IgG1 antibody (antiRSV clone B21M) as a control.26 Antibodies were affinity-purified using MabSelect SuRe resin (Cytiva) according to the manufacturer’s protocol. .. Analytical SEC was performed using a G3000SWxl column (Tosoh Bioscience) in phosphate-buffered saline.
Binding Assay:Article Title: Synonymous sequence design controls the folding, processing, and activity of the therapeutic protein ADAMTS13.
Article Snippet: Their compact size (~15 kDa) and disulfide bond–independent nature makes them exceptionally stable, highly suitable for intracellular applications as well as cost-effective to produce Ankyron-ADAMTS13 binding affinities were determined by biolayer interferometry (BLI) using Octet RED96 (ForteBio). .. Ankyrons (ProImmune) were first captured by Octet Straptavidin biosensors (Sartorius), with binding of ADAMTS13 variants performed in PBS, 2 mg/mL BSA, and 0.02% Tween 20 at various protein concentrations (1.0-0.05 μM) followed by dissociation in same binding buffer. .. Binding kinetics were monitored by Octet Data Acquisition 8.2.0.9 and analyzed using Octet RED software (ForteBio Data Analysis, version 8.2).
Article Title: Proteolytic Activation of T Cell Engagers And Bispecific Proteins Using Modular Coiled-Coil Peptides.
Article Snippet: .. Bio-Layer Interferometry The binding affinity value (KD) of unmodified, masked and unmasked OKT3-Fab and UCHT1-Fab against biotinylated human CD3-epsilon/ delta antigen (purchased from ACROBiosystems) was determined by J using SATORIUS Octet R4 Bio Layer Interferometry (BLI) system. .. The BLI system measures rates of association or binding (ka) and dissociation (kd) of the analytes (here Fab proteins) to the ligand (here CD3-epsilon/ delta) that is immobilized to a streptavidin biosensor.
Software:Article Title: Structural basis for broad orthopoxvirus neutralization by two antibodies targeting distinct L1R epitopes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms EliSpot software 8.0 AID GmbH https://www.aid-diagnostika.com Octet data analysis software 13.0 Sartorius https://www.sartorius.com.cn EPU software 3.0 Thermo Fisher Scientific https://www.thermofisher.com CryoSPARC CryoSPARC https://www.cryosparc.com ChimeraX 1.8 Goddard et al.46 https://www.cgl.ucsf.edu/chimera/ GraphPad Prism version 8.0 GraphPad https://www.graphpad.com ESPript 3.0 Robert et al.47 https://espript.ibcp.fr WinNonlin software 8.8 Certara https://www.certara.com Other HisTrapTM excel column GE Healthcare Cat#29048586 Superdex 200 Increase column GE Healthcare Cat#28990944 SepMateTM-50 (IVD) tubes STEMCELL Technologies Cat#85450 HiTrapTM Protein A HP column GE Healthcare Cat#17040301 SA biosensor Sartorius Cat#18–5019 AHC2 biosensor Sartorius Cat#18–5142 Krios G4 electron microscope Thermo Fisher Scientific https://www.thermofisher.com Cell Reports Medicine 7, 103077, October 20, 2026 e3 Article ll OPEN ACCESS were ultrafiltration concentrated and exchanged in PBS buffer. .. All proteins were checked by SDS-PAGE or high-performance liquid chromatography (HPLC) and quantified using BCA Protein Assay Kit (ThermoFisher Scientific).
Enzyme-linked Immunospot:Article Title: Structural basis for broad orthopoxvirus neutralization by two antibodies targeting distinct L1R epitopes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms EliSpot software 8.0 AID GmbH https://www.aid-diagnostika.com Octet data analysis software 13.0 Sartorius https://www.sartorius.com.cn EPU software 3.0 Thermo Fisher Scientific https://www.thermofisher.com CryoSPARC CryoSPARC https://www.cryosparc.com ChimeraX 1.8 Goddard et al.46 https://www.cgl.ucsf.edu/chimera/ GraphPad Prism version 8.0 GraphPad https://www.graphpad.com ESPript 3.0 Robert et al.47 https://espript.ibcp.fr WinNonlin software 8.8 Certara https://www.certara.com Other HisTrapTM excel column GE Healthcare Cat#29048586 Superdex 200 Increase column GE Healthcare Cat#28990944 SepMateTM-50 (IVD) tubes STEMCELL Technologies Cat#85450 HiTrapTM Protein A HP column GE Healthcare Cat#17040301 SA biosensor Sartorius Cat#18–5019 AHC2 biosensor Sartorius Cat#18–5142 Krios G4 electron microscope Thermo Fisher Scientific https://www.thermofisher.com Cell Reports Medicine 7, 103077, October 20, 2026 e3 Article ll OPEN ACCESS were ultrafiltration concentrated and exchanged in PBS buffer. .. All proteins were checked by SDS-PAGE or high-performance liquid chromatography (HPLC) and quantified using BCA Protein Assay Kit (ThermoFisher Scientific).
Analysis:Article Title: Structural basis for broad orthopoxvirus neutralization by two antibodies targeting distinct L1R epitopes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms EliSpot software 8.0 AID GmbH https://www.aid-diagnostika.com Octet data analysis software 13.0 Sartorius https://www.sartorius.com.cn EPU software 3.0 Thermo Fisher Scientific https://www.thermofisher.com CryoSPARC CryoSPARC https://www.cryosparc.com ChimeraX 1.8 Goddard et al.46 https://www.cgl.ucsf.edu/chimera/ GraphPad Prism version 8.0 GraphPad https://www.graphpad.com ESPript 3.0 Robert et al.47 https://espript.ibcp.fr WinNonlin software 8.8 Certara https://www.certara.com Other HisTrapTM excel column GE Healthcare Cat#29048586 Superdex 200 Increase column GE Healthcare Cat#28990944 SepMateTM-50 (IVD) tubes STEMCELL Technologies Cat#85450 HiTrapTM Protein A HP column GE Healthcare Cat#17040301 SA biosensor Sartorius Cat#18–5019 AHC2 biosensor Sartorius Cat#18–5142 Krios G4 electron microscope Thermo Fisher Scientific https://www.thermofisher.com Cell Reports Medicine 7, 103077, October 20, 2026 e3 Article ll OPEN ACCESS were ultrafiltration concentrated and exchanged in PBS buffer. .. All proteins were checked by SDS-PAGE or high-performance liquid chromatography (HPLC) and quantified using BCA Protein Assay Kit (ThermoFisher Scientific).
Microscopy:Article Title: Structural basis for broad orthopoxvirus neutralization by two antibodies targeting distinct L1R epitopes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms EliSpot software 8.0 AID GmbH https://www.aid-diagnostika.com Octet data analysis software 13.0 Sartorius https://www.sartorius.com.cn EPU software 3.0 Thermo Fisher Scientific https://www.thermofisher.com CryoSPARC CryoSPARC https://www.cryosparc.com ChimeraX 1.8 Goddard et al.46 https://www.cgl.ucsf.edu/chimera/ GraphPad Prism version 8.0 GraphPad https://www.graphpad.com ESPript 3.0 Robert et al.47 https://espript.ibcp.fr WinNonlin software 8.8 Certara https://www.certara.com Other HisTrapTM excel column GE Healthcare Cat#29048586 Superdex 200 Increase column GE Healthcare Cat#28990944 SepMateTM-50 (IVD) tubes STEMCELL Technologies Cat#85450 HiTrapTM Protein A HP column GE Healthcare Cat#17040301 SA biosensor Sartorius Cat#18–5019 AHC2 biosensor Sartorius Cat#18–5142 Krios G4 electron microscope Thermo Fisher Scientific https://www.thermofisher.com Cell Reports Medicine 7, 103077, October 20, 2026 e3 Article ll OPEN ACCESS were ultrafiltration concentrated and exchanged in PBS buffer. .. All proteins were checked by SDS-PAGE or high-performance liquid chromatography (HPLC) and quantified using BCA Protein Assay Kit (ThermoFisher Scientific).
Liquid Chromatography:Article Title: Microbial aldehyde-alcohol dehydrogenase degrades metformin to limit therapeutic response in type 2 diabetes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 μm, 100 Å) Waters Cat# 186003539 Nexera LC-40D x3 UHPLC system Shimadzu LC-40D x3 Triple Quad 5500+ LC-MS/MS System – QTRAP Ready SCIEX Triple Quad 5500+ QTRAP ACQUITY UPLC I-Class PLUS System Waters I-Class PLUS Orbitrap Exploris 120 mass spectrometer Thermo Fisher Scientific Cat# BRE725531; HRAM-MS Monolith NT.115 NanoTemper Technologies Microscale thermophoresis instrument Octet K2 Sartorius / ForteBio Bio-layer interferometry instrument ll OPEN ACCESSArticle Cell Metabolism 38, 1–18.e1–e15, November 3, 2026 e3 Colonization with candidate taxa To test the impact of metformin-modifying species, HFD-fed SPF males received a single gavage of Eubacterium hallii MD1, E. eligens, E. ramulus, E. ventriosum or E. coprostanoligenes (2 × 108 CFU in 0.2 ml sterile anaerobic PBS) or vehicle control. ..
Mass Spectrometry:Article Title: Microbial aldehyde-alcohol dehydrogenase degrades metformin to limit therapeutic response in type 2 diabetes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 μm, 100 Å) Waters Cat# 186003539 Nexera LC-40D x3 UHPLC system Shimadzu LC-40D x3 Triple Quad 5500+ LC-MS/MS System – QTRAP Ready SCIEX Triple Quad 5500+ QTRAP ACQUITY UPLC I-Class PLUS System Waters I-Class PLUS Orbitrap Exploris 120 mass spectrometer Thermo Fisher Scientific Cat# BRE725531; HRAM-MS Monolith NT.115 NanoTemper Technologies Microscale thermophoresis instrument Octet K2 Sartorius / ForteBio Bio-layer interferometry instrument ll OPEN ACCESSArticle Cell Metabolism 38, 1–18.e1–e15, November 3, 2026 e3 Colonization with candidate taxa To test the impact of metformin-modifying species, HFD-fed SPF males received a single gavage of Eubacterium hallii MD1, E. eligens, E. ramulus, E. ventriosum or E. coprostanoligenes (2 × 108 CFU in 0.2 ml sterile anaerobic PBS) or vehicle control. ..
Microscale Thermophoresis:Article Title: Microbial aldehyde-alcohol dehydrogenase degrades metformin to limit therapeutic response in type 2 diabetes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 μm, 100 Å) Waters Cat# 186003539 Nexera LC-40D x3 UHPLC system Shimadzu LC-40D x3 Triple Quad 5500+ LC-MS/MS System – QTRAP Ready SCIEX Triple Quad 5500+ QTRAP ACQUITY UPLC I-Class PLUS System Waters I-Class PLUS Orbitrap Exploris 120 mass spectrometer Thermo Fisher Scientific Cat# BRE725531; HRAM-MS Monolith NT.115 NanoTemper Technologies Microscale thermophoresis instrument Octet K2 Sartorius / ForteBio Bio-layer interferometry instrument ll OPEN ACCESSArticle Cell Metabolism 38, 1–18.e1–e15, November 3, 2026 e3 Colonization with candidate taxa To test the impact of metformin-modifying species, HFD-fed SPF males received a single gavage of Eubacterium hallii MD1, E. eligens, E. ramulus, E. ventriosum or E. coprostanoligenes (2 × 108 CFU in 0.2 ml sterile anaerobic PBS) or vehicle control. ..
Sterility:Article Title: Microbial aldehyde-alcohol dehydrogenase degrades metformin to limit therapeutic response in type 2 diabetes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 μm, 100 Å) Waters Cat# 186003539 Nexera LC-40D x3 UHPLC system Shimadzu LC-40D x3 Triple Quad 5500+ LC-MS/MS System – QTRAP Ready SCIEX Triple Quad 5500+ QTRAP ACQUITY UPLC I-Class PLUS System Waters I-Class PLUS Orbitrap Exploris 120 mass spectrometer Thermo Fisher Scientific Cat# BRE725531; HRAM-MS Monolith NT.115 NanoTemper Technologies Microscale thermophoresis instrument Octet K2 Sartorius / ForteBio Bio-layer interferometry instrument ll OPEN ACCESSArticle Cell Metabolism 38, 1–18.e1–e15, November 3, 2026 e3 Colonization with candidate taxa To test the impact of metformin-modifying species, HFD-fed SPF males received a single gavage of Eubacterium hallii MD1, E. eligens, E. ramulus, E. ventriosum or E. coprostanoligenes (2 × 108 CFU in 0.2 ml sterile anaerobic PBS) or vehicle control. ..
Affinity Constant:Article Title: A reciprocally masked anti-CD47/IL-2 fusion protein promotes antitumor immunity and limits systemic toxicity
Article Snippet: Chromatograms were analyzed using Empower 3 so ware (Waters). .. Affinity measurement Affinity of a-hCD47 and aCD47 were determined by biolayer interferometry using an Octet RED96 instrument (Sartorius). .. An bodies were diluted at 5 μg/mL in 1X Kine c Buffer (KB; Sartorius) and loaded on Protein A biosensors (Sartorius) for 300 seconds.
SPR Assay:
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