cellreporterxpress software (Danaher Inc)
Danaher Inc is a verified supplier 
Danaher Inc manufactures this product 
95
Structured Review
Danaher Inc
cellreporterxpress software
Cellreporterxpress Software, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 95/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analysis+software/ImageXpress+Pico+System+with+CellReporterXpress+Automated+Image+Acquisition+and+Analysis+Software/pmc09776765-130-24-29
Average 95 stars, based on 115 article reviews
Cellreporterxpress Software, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 95/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analysis+software/ImageXpress+Pico+System+with+CellReporterXpress+Automated+Image+Acquisition+and+Analysis+Software/pmc09776765-130-24-29
Average 95 stars, based on 115 article reviews
cellreporterxpress software - by Bioz Stars,
2026-10
95/100 stars
Images
1) Product Images from "‘Shared-Hook’ and ‘Changed-Hook’ Binding Activities of Herpesviral Core Nuclear Egress Complexes Identified by Random Mutagenesis"
Article Title: ‘Shared-Hook’ and ‘Changed-Hook’ Binding Activities of Herpesviral Core Nuclear Egress Complexes Identified by Random Mutagenesis
Journal: Cells
doi: 10.3390/cells11244030
Figure Legend Snippet: Qualitative and quantitative analyses of HCMV AD169-GFP ΔUL53 virus reconstitution on the different recombinant HFF cell populations. HFFs that express pUL53, pUL53-Flag or pUL53::sHook1-Flag were transfected with the infectious bacterial artificial chromosome of HCMV ΔUL53. ( A ) Images of the GFP signals and the respective brightfield illuminations were taken at indicated time points; scale bar in panel A, picture 1 marks 500 μm. ( B ) Quantitative analysis of fluorescence-positive cells was achieved with automated counting by the CellReporterXpress ® software (Molecular Devices LLC, San Jose, CA, USA). Counting parameters for positive nuclei were set to an intensity of at least 100, a minimal width of 7 and a maximal width of 30. Measurements were performed in biological sextuplicates per cell population of 5.05% area of the well and mean values ± SD are given. ( C ) qPCR-based assay for the determination of viral genome equivalents referring to the respective recombinant HFF populations. Viral supernatants were harvested at day 24 p.t. and subjected to IE1-specific qPCR. Calculations were performed in biological sextuplicates per cell population; mean values ± SD are shown. ( B , C ) Statistical analysis was performed using an ordinary two-way ANOVA and post-hoc Tukey correction; ****, p < 0.0001; **, p < 0.01; *, p < 0.1; ns, not significant; no significant differences were noted for values between 1–20 d p.t. ( B ). ( D ) Wb-based expression analysis of the conditionally expressing HFF populations. Recombinant protein expression in the three HFF populations of HFF-UL53, HFF-UL53-Flag and HFF-UL53::sHook1-Flag cells was either uninduced (-) or induced (500 ng/mL Dox). Cells were either infected with HCMV ΔUL53 or remained mock-infected. At 24 h p.t., cells were harvested and lysed. Total lysate samples were subjected to standard Wb analysis using tag-specific or protein-specific monoclonal antibodies as indicated.
Techniques Used: Virus, Recombinant, Transfection, Fluorescence, Software, Expressing, Infection, Bioprocessing
Figure Legend Snippet: Viral replication kinetics of HCMV ΔUL53 and its revertant (HCMV Rev) determined by quantitation of GFP-positive cells and HCMV-specific qPCR on the different recombinant HFF populations. 80,000 inducibly expressing HFFs in 24-well plates were infected with HCMV ΔUL53 or HCMV Rev at a viral dose of 5 × 10 6 genome copies. pUL53, pUL53-Flag or pUL53::sHook1-Flag protein expression was either Dox-induced (+Dox) or remained non-induced (−Dox). ( A ) The number of HCMV-infected cells was measured by detection of GFP signal-positive cells at indicated time points with the CellReporterXpress ® software using the ImageXpress ® Pico device. Values represent 25.04 % of the area of a well and are given as a mean value ± SD of two independently infected wells. ( B ) Viral supernatants were harvested at indicated time points and viral genome equivalents were determined by qPCR. Each value represents the mean ± SD of two independent biological replicates, each measured twice. ( A , B ) Statistical analysis was performed using an ordinary two-way ANOVA and post-hoc Sidak correction; ****, p < 0.0001; ***, p < 0.001; *, p < 0.1; ns, not significant; no significant differences were noted for values between 1–13 d p.i. ( A ) or 1–11 d p.i. ( B ), respectively.
Techniques Used: Quantitation Assay, Recombinant, Expressing, Infection, Software
Related Articles
Fluorescence:Article Title: Multi-parametric profiling of IL-7-augmented GD2.CART products in a phase 1 clinical trial Article Snippet: .. Total area of GFP fluorescence for each condition was measured every 7 h using the ImageXpress Pico device (Molecular Devices) and total GFP area was quantified with Article Title: Advancing Trypanosoma cruzi N-myristoyltransferase as a drug target for Chagas disease through in silico discovery and biochemical evaluation Article Snippet: Cells were treated with 2-fold dilutions ranging from 100 to 0.195 μM of each compound and incubated for 72 h. Following treatment, cell viability was assessed using Hoechst 33,342 (Thermo Fisher Scientific, Cat# H3570) and propidium iodide (PI; Thermo Fisher Scientific, Cat# P3566) staining. .. Fluorescence imaging was performed using the ImageXpress Pico system (Molecular Devices), and viable versus dead cell populations were quantified using Software:Article Title: Multi-parametric profiling of IL-7-augmented GD2.CART products in a phase 1 clinical trial Article Snippet: .. Total area of GFP fluorescence for each condition was measured every 7 h using the ImageXpress Pico device (Molecular Devices) and total GFP area was quantified with Article Title: A young child formula with Limosilactobacillus reuteri and GOS modulates gut microbiome and enhances bone and muscle development: a randomized trial. Article Snippet: The next day, the cells were washed and stained with Hoechst 33342 (Sigma) and secondary antibodies coupled with fluorescent molecules (ThermoFisher Scientific). .. Article Title: Differential effects of KRAS G-domain and hypervariable region mutants on cancer phenotypes. Article Snippet: .. The nuclei were then counterstained with 50 μg/ml Hoechst 33,342 solution for another 30 min prior to image acquisition using the ImageXpress® Pico Automated Cell Imaging System together with the CellReporterXpress Image Acquisition and Article Title: Advancing Trypanosoma cruzi N-myristoyltransferase as a drug target for Chagas disease through in silico discovery and biochemical evaluation Article Snippet: Cells were treated with 2-fold dilutions ranging from 100 to 0.195 μM of each compound and incubated for 72 h. Following treatment, cell viability was assessed using Hoechst 33,342 (Thermo Fisher Scientific, Cat# H3570) and propidium iodide (PI; Thermo Fisher Scientific, Cat# P3566) staining. .. Fluorescence imaging was performed using the ImageXpress Pico system (Molecular Devices), and viable versus dead cell populations were quantified using Article Title: Image cytometry-based quantification protocol of human pulmonary arterial endothelial cells in lab-fabricated multichannel microfluidic devices Article Snippet: GraphPad Prism (version 10.2.3) , Dotmatics, MA, USA , N/A. .. Article Title: Modelling EWS::FLI1 protein fluctuations reveal determinants of tumor plasticity in Ewing sarcoma Article Snippet: Zeiss Zen 2.3 Lite , Zeiss , RRID:SCR_023747. .. Article Title: Bemnifosbuvir and remdesivir inhibit tick-borne encephalitis virus infection in complementary in vitro and ex vivo disease models Article Snippet: .. Fluorescent signal detection and image analysis were carried out using the ImageXpress Pico automated imaging system in combination with Imaging:Article Title: Multi-parametric profiling of IL-7-augmented GD2.CART products in a phase 1 clinical trial Article Snippet: .. Total area of GFP fluorescence for each condition was measured every 7 h using the ImageXpress Pico device (Molecular Devices) and total GFP area was quantified with Article Title: Differential effects of KRAS G-domain and hypervariable region mutants on cancer phenotypes. Article Snippet: .. The nuclei were then counterstained with 50 μg/ml Hoechst 33,342 solution for another 30 min prior to image acquisition using the ImageXpress® Pico Automated Cell Imaging System together with the CellReporterXpress Image Acquisition and Article Title: Advancing Trypanosoma cruzi N-myristoyltransferase as a drug target for Chagas disease through in silico discovery and biochemical evaluation Article Snippet: Cells were treated with 2-fold dilutions ranging from 100 to 0.195 μM of each compound and incubated for 72 h. Following treatment, cell viability was assessed using Hoechst 33,342 (Thermo Fisher Scientific, Cat# H3570) and propidium iodide (PI; Thermo Fisher Scientific, Cat# P3566) staining. .. Fluorescence imaging was performed using the ImageXpress Pico system (Molecular Devices), and viable versus dead cell populations were quantified using Article Title: Bemnifosbuvir and remdesivir inhibit tick-borne encephalitis virus infection in complementary in vitro and ex vivo disease models Article Snippet: .. Fluorescent signal detection and image analysis were carried out using the ImageXpress Pico automated imaging system in combination with Microscopy:Article Title: A young child formula with Limosilactobacillus reuteri and GOS modulates gut microbiome and enhances bone and muscle development: a randomized trial. Article Snippet: The next day, the cells were washed and stained with Hoechst 33342 (Sigma) and secondary antibodies coupled with fluorescent molecules (ThermoFisher Scientific). .. |