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Structured Review

Bio-Rad primepcr
Primepcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analysis+software/PrimePCR+Analysis+Software/pmc06829596-218-11-12
Average 99 stars, based on 217 article reviews
primepcr - by Bioz Stars, 2026-09
99/100 stars

Images

Related Articles

Staining:

Article Title: Isothermal and protein-free cascade catalytic hairpin assembly induced-DNAzyme sensing strategy for sensitive miRNA analysis
Article Snippet: Electrophoresis was performed using a gel electrophoresis unit (Bio-Rad Laboratories, USA) submerged in 1× TBE running buffer (89 mM Tris-borate, 2 mM EDTA, pH 8.3). .. Separation was carried out at a constant voltage of 80 V for 70 min. Post-electrophoresis, the gel was immersed in a diluted solution of Super GelRedTM nucleic acid stain for 20 min. Gel images were subsequently captured and documented using a Gel DocTM EZ system equipped with Image LabTM imaging and analysis software (Bio-Rad Laboratories, USA). ..

Article Title: Isothermal and protein-free cascade catalytic hairpin assembly induced-DNAzyme sensing strategy for sensitive miRNA analysis.
Article Snippet: Electrophoresis was performed using a gel electrophoresis unit (Bio-Rad Laboratories, USA) submerged in 1× TBE running buffer (89 mM Tris-borate, 2 mM EDTA, pH 8.3). .. Separation was carried out at a constant voltage of 80 V for 70 min. Post-electrophoresis, the gel was immersed in a diluted solution of Super GelRedTM nucleic acid stain for 20 min. Gel images were subsequently captured and documented using a Gel DocTM EZ system equipped with Image LabTM imaging and analysis software (Bio-Rad Laboratories, USA). ..

Imaging:

Article Title: Isothermal and protein-free cascade catalytic hairpin assembly induced-DNAzyme sensing strategy for sensitive miRNA analysis
Article Snippet: Electrophoresis was performed using a gel electrophoresis unit (Bio-Rad Laboratories, USA) submerged in 1× TBE running buffer (89 mM Tris-borate, 2 mM EDTA, pH 8.3). .. Separation was carried out at a constant voltage of 80 V for 70 min. Post-electrophoresis, the gel was immersed in a diluted solution of Super GelRedTM nucleic acid stain for 20 min. Gel images were subsequently captured and documented using a Gel DocTM EZ system equipped with Image LabTM imaging and analysis software (Bio-Rad Laboratories, USA). ..

Article Title: Isothermal and protein-free cascade catalytic hairpin assembly induced-DNAzyme sensing strategy for sensitive miRNA analysis.
Article Snippet: Electrophoresis was performed using a gel electrophoresis unit (Bio-Rad Laboratories, USA) submerged in 1× TBE running buffer (89 mM Tris-borate, 2 mM EDTA, pH 8.3). .. Separation was carried out at a constant voltage of 80 V for 70 min. Post-electrophoresis, the gel was immersed in a diluted solution of Super GelRedTM nucleic acid stain for 20 min. Gel images were subsequently captured and documented using a Gel DocTM EZ system equipped with Image LabTM imaging and analysis software (Bio-Rad Laboratories, USA). ..

Software:

Article Title: Isothermal and protein-free cascade catalytic hairpin assembly induced-DNAzyme sensing strategy for sensitive miRNA analysis
Article Snippet: Electrophoresis was performed using a gel electrophoresis unit (Bio-Rad Laboratories, USA) submerged in 1× TBE running buffer (89 mM Tris-borate, 2 mM EDTA, pH 8.3). .. Separation was carried out at a constant voltage of 80 V for 70 min. Post-electrophoresis, the gel was immersed in a diluted solution of Super GelRedTM nucleic acid stain for 20 min. Gel images were subsequently captured and documented using a Gel DocTM EZ system equipped with Image LabTM imaging and analysis software (Bio-Rad Laboratories, USA). ..

Article Title: Shen-Ling-Bai-Zhu-San alleviates ulcerative colitis by enhancing mitophagy via the Nrf2/PINK1/Parkin pathway.
Article Snippet: weight loss, diarrhea, rectal bleeding, abdominal pain, and mucosal inflammation that spreads from the rectum to the distal colon(Chen et al. 2023).. Currently, 5-aminosalicylic acid drugs, corticosteroids, immunosuppressants, and biologics represent the most prevalent non-surgical treatment options for UC, but the one-year clinical remission rate hovers at merely around 40% (Liang et al. 2024).. Surgical intervention presents itself as the ultimate remedy for eradicating UC, but postoperative complications like stenosis and anastomotic fistula significantly undermine the patients’ quality of life (Kobayashi et al. 2020).

Article Title: Isothermal and protein-free cascade catalytic hairpin assembly induced-DNAzyme sensing strategy for sensitive miRNA analysis.
Article Snippet: Electrophoresis was performed using a gel electrophoresis unit (Bio-Rad Laboratories, USA) submerged in 1× TBE running buffer (89 mM Tris-borate, 2 mM EDTA, pH 8.3). .. Separation was carried out at a constant voltage of 80 V for 70 min. Post-electrophoresis, the gel was immersed in a diluted solution of Super GelRedTM nucleic acid stain for 20 min. Gel images were subsequently captured and documented using a Gel DocTM EZ system equipped with Image LabTM imaging and analysis software (Bio-Rad Laboratories, USA). ..

Article Title: Surface Acoustic Waves-Based Molecular Recognition of a Collagen Receptor on Human Erythrocytes.
Article Snippet: The membranes were incubated for 45 min with horseradish peroxidase (HRP) linked secondary antibody diluted in blocking buffer (Table 3) after 5 washes with deionized water and 5 min incubation with TBS-T. Then the membranes were washed 7 times with deionized water and incubated for 5 min in TBS-T. At the end, the chemiluminescent HRP substrate (Millipore, Burlington, MA, USA) was applied to the blots for 5 min, initiating the chemiluminescence reaction catalyzed by the peroxidase conjugated to the secondary antibody. .. The emitted light was detected with a ChemiDoc XRS system (Bio-Rad, Hercules, CA, USA); the image acquisition was conducted using Quantity One 1-D Analysis software v4.6.9 (Bio-Rad, Hercules, CA, USA). .. FIJI-ImageJ 2.0.0-rc-2 software was used for image processing analysis [68].

Variant Assay:

Article Title: Harnessing liquid biopsy to unveil RAS-MEK pathway somatic pathogenic variants in extracranial arterio-venous malformations.
Article Snippet: .. Orthogonal confirmation ddPCR –NM_004985(KRAS):c.35 G > A (p.G12D) [KRAS p.G12D]was analysed using PrimePCR variant detection assay dHsaMDV2510596 (KRAS:c.35 G > A, p.G12D, 6-FAM, KRAS WT reference gene, HEX; Bio-Rad Laboratories) as described in Kahana-Edwin et al., 202119. .. Controls included a non-template control (NTC), which contained purified water instead of cfDNA, and the artificial cfDNA reference standards described above (Horizon Discovery).

Article Title: Harnessing liquid biopsy to unveil RAS-MEK pathway somatic pathogenic variants in extracranial arterio-venous malformations
Article Snippet: .. ddPCR – NM_004985 (KRAS):c.35 G > A (p.G12D) [KRAS p.G12D] was analysed using PrimePCR variant detection assay dHsaMDV2510596 (KRAS:c.35 G > A, p.G12D, 6-FAM, KRAS WT reference gene, HEX; Bio-Rad Laboratories) as described in Kahana-Edwin et al., 2021 . .. Controls included a non-template control (NTC), which contained purified water instead of cfDNA, and the artificial cfDNA reference standards described above (Horizon Discovery).

Detection Assay:

Article Title: Harnessing liquid biopsy to unveil RAS-MEK pathway somatic pathogenic variants in extracranial arterio-venous malformations.
Article Snippet: .. Orthogonal confirmation ddPCR –NM_004985(KRAS):c.35 G > A (p.G12D) [KRAS p.G12D]was analysed using PrimePCR variant detection assay dHsaMDV2510596 (KRAS:c.35 G > A, p.G12D, 6-FAM, KRAS WT reference gene, HEX; Bio-Rad Laboratories) as described in Kahana-Edwin et al., 202119. .. Controls included a non-template control (NTC), which contained purified water instead of cfDNA, and the artificial cfDNA reference standards described above (Horizon Discovery).

Article Title: Harnessing liquid biopsy to unveil RAS-MEK pathway somatic pathogenic variants in extracranial arterio-venous malformations
Article Snippet: .. ddPCR – NM_004985 (KRAS):c.35 G > A (p.G12D) [KRAS p.G12D] was analysed using PrimePCR variant detection assay dHsaMDV2510596 (KRAS:c.35 G > A, p.G12D, 6-FAM, KRAS WT reference gene, HEX; Bio-Rad Laboratories) as described in Kahana-Edwin et al., 2021 . .. Controls included a non-template control (NTC), which contained purified water instead of cfDNA, and the artificial cfDNA reference standards described above (Horizon Discovery).

Quantitative RT-PCR:

Article Title: Exploring the differentiation potential of adipose tissue-derived mesenchymal stromal/stem cells and progenitor buccal epithelial cells into urothelial cells
Article Snippet: .. RT-qPCR was performed using PrimePCRTM primers ( , Bio-Rad, Hercules, CA, United States) and the LightCycler 480 SYBR Green I Master Kit (Roche Diagnostics, Basel, Switzerland). ..

SYBR Green Assay:

Article Title: Exploring the differentiation potential of adipose tissue-derived mesenchymal stromal/stem cells and progenitor buccal epithelial cells into urothelial cells
Article Snippet: .. RT-qPCR was performed using PrimePCRTM primers ( , Bio-Rad, Hercules, CA, United States) and the LightCycler 480 SYBR Green I Master Kit (Roche Diagnostics, Basel, Switzerland). ..



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Image Search Results


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Journal: STAR Protocols

Article Title: Protocol for Seahorse 3D Mito Stress assay in patient-derived atypical teratoid rhabdoid tumor CHLA-05-ATRT single neurospheres

doi: 10.1016/j.xpro.2026.104515

Figure Lengend Snippet: Oxygen consumption rate (OCR) in the presence or absence of spheroids (A) Incucyte image of spheroid in the center of the microplate well at the end of the assay and (B) the corresponding OCR kinetic graph showing a response after drug injections as expected. (C) Image of a microplate well with no spheroid detected at the end of the assay and (D) corresponding OCR kinetic graph showing no response after drug injections.

Article Snippet: Incucyte Organoid Analysis Software Module , Sartorius , 9600–0034.

Techniques:

Effect of coating timing on spheroid retention in microplate wells (A) Incucyte image of a poly-lysine coated microplate with spheroids transferred on the same day as the assay, taken before the assay. (B) Image of the same plate taken after the assay, showing that the spheroids were displaced and/or destroyed during the assay. (C) Image of a poly-lysine coated microplate with spheroids transferred the day before the assay, taken before the assay and (D) after the assay, showing that the spheroids were intact.

Journal: STAR Protocols

Article Title: Protocol for Seahorse 3D Mito Stress assay in patient-derived atypical teratoid rhabdoid tumor CHLA-05-ATRT single neurospheres

doi: 10.1016/j.xpro.2026.104515

Figure Lengend Snippet: Effect of coating timing on spheroid retention in microplate wells (A) Incucyte image of a poly-lysine coated microplate with spheroids transferred on the same day as the assay, taken before the assay. (B) Image of the same plate taken after the assay, showing that the spheroids were displaced and/or destroyed during the assay. (C) Image of a poly-lysine coated microplate with spheroids transferred the day before the assay, taken before the assay and (D) after the assay, showing that the spheroids were intact.

Article Snippet: Incucyte Organoid Analysis Software Module , Sartorius , 9600–0034.

Techniques: