Journal: Aging Cell

Article Title: uPAR ‐Targeting T Cell Engager Exerts Senolytic Effects in Mice and Non‐Human Primates With Serum Aminotransferase Activity as a Safety Monitor

doi: 10.1111/acel.70742

Figure Lengend Snippet: Physical attributes of naturally aged mice were enhanced following mGFD‐CD3 treatment. (A) Maximal walking speed, treadmill endurance, hanging endurance, and grip strength of naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded at 6 months after the first infusion ( n = 5 mice per condition). (B) H&E staining of gastrocnemius collected from mice treated with mCD3 or mGFD‐CD3, sampled 6 months after the first infusion. Scale bar, 50 μm. Relative cell areas in the gastrocnemius were quantified using Image J ( n = 5 mice per condition). (C) Representative micro‐CT images of the distal femurs of the mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion. The relative bone volume fraction (BV/TV) of cancellous bone in the distal femur was quantified for each sample and analyzed by Imaris image analysis software ( n = 5 mice per condition). (D) The percentages of total lean mass and total adipose mass and the adipose mass/lean mass ratio of naturally aged mice treated with mCD3 or mGFD‐CD3 6 months after the first infusion were recorded using InAlyzer software ( n = 5 mice per condition). (E) (left) Micro‐CT images of SAT and bone of the naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded 6 months after the first infusion. Green, SAT; red, bone. (right) Quantification of the adipose tissue of mice based on the micro‐CT images ( left ). Data were normalized to mouse body weight ( n = 5 mice per condition). (F) Basal glucose levels after 12 h of starvation in mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion ( n = 5 mice per condition). (G) ( upper ) Levels of glucose before (0 min) and after the intraperitoneal administration of glucose (2 g per kg body weight) (samples as per in (f)). ( lower ) Area under the curve (AUC) for the results from (g) ( n = 5 mice per condition). The mouse experiments are representative results from two independent experiments. Data are presented as mean ± SD and analyzed by one‐way ANOVA followed by Tukey's multiple comparisons test (A–G). * p ≤ 0.05; ** p ≤ 0.01.

Article Snippet: 3D image analysis was performed with Imaris image analysis software (version 10.1.0).

Techniques: Staining, Micro-CT, Analysis, Software, Tissue