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Life Sciences Advanced Technologies amv reverse transcriptase
Amv Reverse Transcriptase, supplied by Life Sciences Advanced Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amv+reverse+transcriptase/amv+reverse+transcriptase/pmc03390621-103-25-35
Average 90 stars, based on 1 article reviews
amv reverse transcriptase - by Bioz Stars, 2026-09
90/100 stars

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other:

Article Title: IF2 and unique features of initiator tRNA fMet help establish the translational reading frame
Article Snippet: Reverse transcriptase (2 U, Life Sciences Advanced Technologies, Inc.) and dNTPs (0.2 mM each) were then added to extend the primer, and the cDNA products were resolved using 7% PAGE under denaturing (8M urea) conditions.

Article Title: A structural element within the 5′UTR of β-catenin mRNA modulates its translation under hypoxia
Article Snippet: Reverse transcription reactions were performed using avian myeloblastosis virus (AMV) reverse transcriptase (Life Sciences Advanced Technologies) and 5′ fluorescently labelled primers (refer to for sequences) (Thermo Fisher Scientific) at 42°C for 30 min. One more primer extension reaction was performed using unmodified RNA transcript as sequencing reaction, in the presence of 0.25 mM of ddGTP.

Article Title: A structural element within the 5'UTR of β-catenin mRNA modulates its translation under hypoxia.
Article Snippet: Reverse transcription reactions were perormed using avian myeloblastosis virus (AMV) reverse trancriptase (Life Sciences Advanced Technologies) and 5′ fluoescently labelled primers (refer to Supplementary Table S1 or sequences) (Thermo Fisher Scientific) at 42◦C for 30 min. ne more primer extension reaction was performed using unodified RNA transcript as sequencing reaction, in the presnce of 0.25 mM of ddGTP.

Reverse Transcription:

Article Title: σ28-dependent small RNA regulation of flagella biosynthesis
Article Snippet: After the addition of dNTPs (1 mM each) and AMV reverse transcriptase (10 U, Life Sciences Advanced Technologies Inc), the reactions were incubated in a 10 μl- reaction volume at 42 °C for 1 hr. .. After the addition of dNTPs (1 mM each) and AMV reverse transcriptase (10 U, Life Sciences Advanced Technologies Inc), the reactions were incubated in a 10 μl- reaction volume at 42 °C for 1 hr. ..

Article Title: σ 28 -dependent small RNA regulation of flagella biosynthesis
Article Snippet: .. After the addition of dNTPs (1 mM each) and AMV reverse transcriptase (10 U, Life Sciences Advanced Technologies Inc), the reactions were incubated in a 10 μl-reaction volume at 42 °C for 1 hr. ..

Article Title: Rice novel useful semidwarf gene d60 on chromosome 2 causing pleiotropically gamete abortion
Article Snippet: Total RNA was extracted from the leaves using the Acid Phenol Guanidium Chloroform method ( ) and was then treated with 10 U of DNase I (Takara Bio Inc., Kyoto, Japan). poly(A)+ RNA was separated and purified from total RNA using an oligo(dT) cellulose column Poly(A) Quick mRNA Isolation Kit (Stratagene, La Jolla, CA). .. Single-stranded cDNAs were synthesized by reverse transcription reaction using 0.25 U AMV reverse transcriptase (Life Sciences Advanced Technologies, Saint Petersburg, FL) for 30 min at 50°C by using 200 ng of poly(A)+RNA as the template of 2.5 μM oligo(dT) primers in 20 μL solution (1 mM dNTP, 5 mM MgCl 2 . ..

Article Title: A 3’ UTR-derived small RNA connecting nitrogen and carbon metabolism in enteric bacteria
Article Snippet: .. Each sample was then incubated with 0.5 μl AMV Reverse Transcriptase (Life Sciences Advanced Technologies Inc) and 2.5 μM of each dNTP for 1 h at 42°C. .. The sequencing ladder was generated using a Sequenase kit (Thermo Fisher) with aceE DNA amplified by PCR as the template, following the protocol provided.

Article Title: A 3′ UTR-derived small RNA connecting nitrogen and carbon metabolism in enteric bacteria
Article Snippet: .. Each sample was then incubated with 0.5 μl AMV reverse transcriptase (Life Sciences Advanced Technologies Inc) and 2.5 μM of each dNTP for 1 h at 42°C. .. The sequencing ladder was generated using a Sequenase kit (Thermo Fisher) with aceE DNA amplified by PCR as the template, following the protocol provided.

Incubation:

Article Title: σ28-dependent small RNA regulation of flagella biosynthesis
Article Snippet: After the addition of dNTPs (1 mM each) and AMV reverse transcriptase (10 U, Life Sciences Advanced Technologies Inc), the reactions were incubated in a 10 μl- reaction volume at 42 °C for 1 hr. .. After the addition of dNTPs (1 mM each) and AMV reverse transcriptase (10 U, Life Sciences Advanced Technologies Inc), the reactions were incubated in a 10 μl- reaction volume at 42 °C for 1 hr. ..

Article Title: σ 28 -dependent small RNA regulation of flagella biosynthesis
Article Snippet: .. After the addition of dNTPs (1 mM each) and AMV reverse transcriptase (10 U, Life Sciences Advanced Technologies Inc), the reactions were incubated in a 10 μl-reaction volume at 42 °C for 1 hr. ..

Article Title: A 3’ UTR-derived small RNA connecting nitrogen and carbon metabolism in enteric bacteria
Article Snippet: .. Each sample was then incubated with 0.5 μl AMV Reverse Transcriptase (Life Sciences Advanced Technologies Inc) and 2.5 μM of each dNTP for 1 h at 42°C. .. The sequencing ladder was generated using a Sequenase kit (Thermo Fisher) with aceE DNA amplified by PCR as the template, following the protocol provided.

Article Title: A 3′ UTR-derived small RNA connecting nitrogen and carbon metabolism in enteric bacteria
Article Snippet: .. Each sample was then incubated with 0.5 μl AMV reverse transcriptase (Life Sciences Advanced Technologies Inc) and 2.5 μM of each dNTP for 1 h at 42°C. .. The sequencing ladder was generated using a Sequenase kit (Thermo Fisher) with aceE DNA amplified by PCR as the template, following the protocol provided.

Synthesized:

Article Title: Rice novel useful semidwarf gene d60 on chromosome 2 causing pleiotropically gamete abortion
Article Snippet: Total RNA was extracted from the leaves using the Acid Phenol Guanidium Chloroform method ( ) and was then treated with 10 U of DNase I (Takara Bio Inc., Kyoto, Japan). poly(A)+ RNA was separated and purified from total RNA using an oligo(dT) cellulose column Poly(A) Quick mRNA Isolation Kit (Stratagene, La Jolla, CA). .. Single-stranded cDNAs were synthesized by reverse transcription reaction using 0.25 U AMV reverse transcriptase (Life Sciences Advanced Technologies, Saint Petersburg, FL) for 30 min at 50°C by using 200 ng of poly(A)+RNA as the template of 2.5 μM oligo(dT) primers in 20 μL solution (1 mM dNTP, 5 mM MgCl 2 . ..



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