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CHIMERx Inc amv reverse transcriptase
Amv Reverse Transcriptase, supplied by CHIMERx Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amv+reverse+transcriptase/amv+reverse+transcriptase/pmc02720740-88-36-44
Average 90 stars, based on 1 article reviews
amv reverse transcriptase - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Methods of inducing regulated pancreatic hormone production in non-pancreatic islet tissues
Article Snippet: RNA samples were treated by 10 units of RQ1 RNase-free DNaseI (Promega), for 60 minutes. cDNA was prepared by reverse transcription (Native AMV Reverse Transcriptase, Chimerx), using 4 μg DNA-free total RNA and 0.5 μg oligo(dT)15.

Reverse Transcription:

Article Title: Fungal transformation of Colletotrichum coccodes with bacterial oahA to suppress defenses of Abutilon theophrasti
Article Snippet: Abutilon theophrasti (Malvaceae) is a widely distributed weed that infests a variety of agricultural crops.. Cotton is in the same botanical family, precluding the use of selective chemical herbicides.. An Abutilonspecific pathovar of Colletotrichum coccodes has been considered as a potential bioherbicide for the selective control of A. theophrasti.

Article Title: Co-ordinated regulation of flowering time, plant architecture and growth by FASCICULATE : the pepper orthologue of SELF PRUNING
Article Snippet: For first-strand cDNA synthesis, total RNA extracted with the RNeasy plant mini kit (Qiagen) was used. .. The RNA was reverse-transcribed with AMV reverse transcriptase (CHIMERx) using random primers. ..

Article Title: Differential Expression of Novel Adiponectin Receptor-1 Transcripts in Skeletal Muscle of Subjects With Normal Glucose Tolerance and Type 2 Diabetes
Article Snippet: Total RNA samples from human, monkey, and mouse tissues (all male) were purchased from BioChain (Hayward, CA). .. First-strand cDNA was synthesized from 1 μg total RNA using 2.5 units of Super AMV Reverse Transcriptase (CHIMERx, Madison, WI), 10 pmol dT 15 , and 30 units of RNasin (Promega, Madison, WI) in 20 μL reaction buffer. cDNA was amplified by PCR using ReddyMix PCR Master Mix (Thermo-Scientific, Waltham, MA) in the presence of 250 nmol/L primers. ..

Article Title: A nuclear factor-binding domain in the 5'-untranslated region of the amyloid precursor protein promoter: Implications for the regulation of gene expression
Article Snippet: .. The solution was heated to 99°C for 5 minutes, incubated at 61°C for 5 min, at 57°C for 10 min, and at 48°C for 5 min. To each annealing mixture was added 40 μl of a reverse transcriptase reaction mixture containing 12.5 mM Tris, pH 8.0, 10 mM MgCl 2 , 12.5 mM DTT, 1.25 mM each of the dNTPs, 20 units of AMV reverse transcriptase (CHIMERx), and 10 units of RNasin (Invitrogen). .. The reaction was continued at 48°C for 90 min. PCR amplification of 4 μl aliquots of the reverse transcription reaction products was assembled in 40 μl of Mg+ PCR buffer (Roche) supplemented with 0.2 mM of each dNTP, 0.4 pmol each of 5' end-labeled (50-100 × 10 3 cpm) CAT-R, Gal-R, unlabeled CAT-F (5'-TTTCAGGAGCTAAGGAAGCTAAAATGGAG) and Gal-F (5'-CTGAGCCGCGATATTGCCCAG) primers.

Article Title: Recapitulating Cross-Species Transmission of Simian Immunodeficiency Virus SIVcpz to Humans by Using Humanized BLT Mice
Article Snippet: The RT was measured in triplicate using the EnzChek reverse transcriptase assay kit (Invitrogen, Eugene, OR, USA). .. The virus strains were lysed using 10% Triton X-100 (final concentration, 1%) in RPMI medium supplemented with 10% fetal bovine serum (FBS), and HIV-1 reverse transcriptase (CHIMERx, Milwaukee, WI, USA) was used for the standard curve. ..

Article Title: Nanonized black soybean enhances immune response in senescence-accelerated mice
Article Snippet: The RT-PCR assays were performed with a TitanTM One Tube RT-PCR System kit (Boehringer, Mannheim, Germany). .. Briefly, 1 μg of total RNA from each sample was added to 50 μl of a reaction mixture containing 0.2 mM dNTP, 0.4 μM each of sense and antisense specific primers, 5 mM DTT, 5 U RNase inhibitor, 1 μl of AMV reverse transcriptase (Chimerx, Madison, Wis, USA) and ExpandTM High Fidelity enzyme mix (Roche, Taiwan). .. Taq DNA polymerase (5 U/μl, Viogene, Sunnyvale, CA, USA) was then added to the tube.

Synthesized:

Article Title: Differential Expression of Novel Adiponectin Receptor-1 Transcripts in Skeletal Muscle of Subjects With Normal Glucose Tolerance and Type 2 Diabetes
Article Snippet: Total RNA samples from human, monkey, and mouse tissues (all male) were purchased from BioChain (Hayward, CA). .. First-strand cDNA was synthesized from 1 μg total RNA using 2.5 units of Super AMV Reverse Transcriptase (CHIMERx, Madison, WI), 10 pmol dT 15 , and 30 units of RNasin (Promega, Madison, WI) in 20 μL reaction buffer. cDNA was amplified by PCR using ReddyMix PCR Master Mix (Thermo-Scientific, Waltham, MA) in the presence of 250 nmol/L primers. ..

Amplification:

Article Title: Differential Expression of Novel Adiponectin Receptor-1 Transcripts in Skeletal Muscle of Subjects With Normal Glucose Tolerance and Type 2 Diabetes
Article Snippet: Total RNA samples from human, monkey, and mouse tissues (all male) were purchased from BioChain (Hayward, CA). .. First-strand cDNA was synthesized from 1 μg total RNA using 2.5 units of Super AMV Reverse Transcriptase (CHIMERx, Madison, WI), 10 pmol dT 15 , and 30 units of RNasin (Promega, Madison, WI) in 20 μL reaction buffer. cDNA was amplified by PCR using ReddyMix PCR Master Mix (Thermo-Scientific, Waltham, MA) in the presence of 250 nmol/L primers. ..

Polymerase Chain Reaction:

Article Title: Differential Expression of Novel Adiponectin Receptor-1 Transcripts in Skeletal Muscle of Subjects With Normal Glucose Tolerance and Type 2 Diabetes
Article Snippet: Total RNA samples from human, monkey, and mouse tissues (all male) were purchased from BioChain (Hayward, CA). .. First-strand cDNA was synthesized from 1 μg total RNA using 2.5 units of Super AMV Reverse Transcriptase (CHIMERx, Madison, WI), 10 pmol dT 15 , and 30 units of RNasin (Promega, Madison, WI) in 20 μL reaction buffer. cDNA was amplified by PCR using ReddyMix PCR Master Mix (Thermo-Scientific, Waltham, MA) in the presence of 250 nmol/L primers. ..

Incubation:

Article Title: A nuclear factor-binding domain in the 5'-untranslated region of the amyloid precursor protein promoter: Implications for the regulation of gene expression
Article Snippet: .. The solution was heated to 99°C for 5 minutes, incubated at 61°C for 5 min, at 57°C for 10 min, and at 48°C for 5 min. To each annealing mixture was added 40 μl of a reverse transcriptase reaction mixture containing 12.5 mM Tris, pH 8.0, 10 mM MgCl 2 , 12.5 mM DTT, 1.25 mM each of the dNTPs, 20 units of AMV reverse transcriptase (CHIMERx), and 10 units of RNasin (Invitrogen). .. The reaction was continued at 48°C for 90 min. PCR amplification of 4 μl aliquots of the reverse transcription reaction products was assembled in 40 μl of Mg+ PCR buffer (Roche) supplemented with 0.2 mM of each dNTP, 0.4 pmol each of 5' end-labeled (50-100 × 10 3 cpm) CAT-R, Gal-R, unlabeled CAT-F (5'-TTTCAGGAGCTAAGGAAGCTAAAATGGAG) and Gal-F (5'-CTGAGCCGCGATATTGCCCAG) primers.

Virus:

Article Title: Recapitulating Cross-Species Transmission of Simian Immunodeficiency Virus SIVcpz to Humans by Using Humanized BLT Mice
Article Snippet: The RT was measured in triplicate using the EnzChek reverse transcriptase assay kit (Invitrogen, Eugene, OR, USA). .. The virus strains were lysed using 10% Triton X-100 (final concentration, 1%) in RPMI medium supplemented with 10% fetal bovine serum (FBS), and HIV-1 reverse transcriptase (CHIMERx, Milwaukee, WI, USA) was used for the standard curve. ..

Concentration Assay:

Article Title: Recapitulating Cross-Species Transmission of Simian Immunodeficiency Virus SIVcpz to Humans by Using Humanized BLT Mice
Article Snippet: The RT was measured in triplicate using the EnzChek reverse transcriptase assay kit (Invitrogen, Eugene, OR, USA). .. The virus strains were lysed using 10% Triton X-100 (final concentration, 1%) in RPMI medium supplemented with 10% fetal bovine serum (FBS), and HIV-1 reverse transcriptase (CHIMERx, Milwaukee, WI, USA) was used for the standard curve. ..



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