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Sangon Biotech amv reverse transcriptase
Amv Reverse Transcriptase, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amv+reverse+transcriptase/leukemia+m+mlv+moloney+murine+reverse+transcriptase+virus/pm42026873-40-12-16
Average 86 stars, based on 1 article reviews
amv reverse transcriptase - by Bioz Stars, 2026-11
86/100 stars

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Reverse Transcription:

Article Title: Lateral Flow Biosensor for On-Site Multiplex Detection of Viruses Based on One-Step Reverse Transcription and Strand Displacement Amplification.
Article Snippet: .. Oligonucleotides, deoxynucleoside triphosphates (dNTPs) and AMV Reverse Transcriptase were purchased from Shanghai Sangon Biological Engineering Technology (Shang- Biosensors 2024, 14, 103 4 of 13 hai, China). ..

Article Title: Loss of CA12 drive proliferation and fibrosis in type i congenital pulmonary airway malformation via promoting abnormal activation of SHH signaling
Article Snippet: Total RNA was extracted from cyst-adjacent healthy lung tissues and hyperplastic cystic lesions from patients with type 1 CPAM utilizing the Trizol (Shanghai Sangon Biotech, China). .. Reverse transcription was performed with 1 μg of total RNA using the HiScript II Reverse Transcriptase (Shanghai Sangon Biotech) and oligo(dT)18 primers. ..

Article Title: Assessment on intestinal health from polylactic acid microplastics degradation on rare minnow (Gobiocypris rarus): Inflammation regulation by mitochondrial dysfunction.
Article Snippet: Biodegradable plastics (BPs), a class of polymers designed to break down under natural environmental conditions, are currently effective measures to deal with the crisis of "white pollution" (the persistent accumulation of non-degradable plastic waste in the environment).. Despite their environmental promise, growing concerns have emerged regarding the potential ecological risks of BPs.. However, the health risks and its underlying mechanism of natural degradable BPs on intestinal tissues remain unclear.

Article Title: Sestrin2 emerges as a regulator of ROS-mediated oxidative stress and apoptosis in the cadmium-induced liver injury of rare minnow (Gobiocypris rarus).
Article Snippet: The total RNA of the liver tissues (n = 2) and cells from each sample was isolated by using TRIZOL reagent (Invitrogen, Thermo Fisher Scientific) according to manufacturer’s instructions. .. First strand cDNA was transcribed from 1 μg of total RNA by Moloney murine leukemia virus (M-MLV) reverse transcriptase (Sangon Biotech, Shanghai, China). .. In brief, cDNA was synthesized by using a SYBR Green PCR master mix (Bimake, Houston, TX, USA) in an ABI7500 real-time PCR instrument (Bio-Rad, Califormia, USA).

Article Title: TPX2 promotes gastric cancer progression and angiogenesis via Wnt/β-catenin signaling
Article Snippet: .. The extraction of total RNA from GC tissues and cells was initially performed using the Trizol reagent (15596018CN, Invitrogen), and cDNA was subsequently generated through reverse transcription with AMV reverse transcriptase (B600020, Sangon Biotech). .. PCR amplification of the cDNA was then conducted using TB Green FAST qPCR Kit (CN830S, TAKARA, Tokyo, Japan).

Article Title: Ciliatoside A Improves Klebsiella pneumoniae-Induced Pneumonia by Modulating Mitochondrial Autophagy via the SIRT1/PINK1/Parkin Pathway.
Article Snippet: Background: Klebsiella pneumoniae (KP)induced pneumonia has a high incidence rate, and current treatment options remain limited.. The efficacy and mechanism of the novel natural compound Ciliatoside A (CA) against KPinduced pneumonia remain

Article Title: Parental exposure to environmental cadmium causes developmental defects in the rare minnow (Gobiocypris rarus) embryos: The association between ROS-mediated oxidative stress and developmental dysfunction
Article Snippet: Cadmium is widely distributed in aquaculture systems.. The exposure of parental fish species to cadmium is known to retard of growth and cause death in offspring.. However, the potential mechanisms responsible for how parental cadmium exposure effects embryonic development yet to be elucidated.

Article Title: LST1: a novel biomarker for efferocytosis in the co-occurrence of type 2 diabetes mellitus and clear cell renal cell carcinoma
Article Snippet: .. Total RNA was extracted from cells using Trizol reagent, followed by reverse transcription with M-MuLV reverse transcriptase from Sangon Biotech (Shanghai) to synthesize cDNA. .. PCR amplification of the target genes was conducted using the SYBR Green real-time quantitative PCR system (StepOne Real-Time PCR System) from Sangon Biotech (Shanghai) and the StepOne Software v2.3 on a quantitative PCR instrument from ABI (USA).

Virus:

Article Title: Assessment on intestinal health from polylactic acid microplastics degradation on rare minnow (Gobiocypris rarus): Inflammation regulation by mitochondrial dysfunction.
Article Snippet: Biodegradable plastics (BPs), a class of polymers designed to break down under natural environmental conditions, are currently effective measures to deal with the crisis of "white pollution" (the persistent accumulation of non-degradable plastic waste in the environment).. Despite their environmental promise, growing concerns have emerged regarding the potential ecological risks of BPs.. However, the health risks and its underlying mechanism of natural degradable BPs on intestinal tissues remain unclear.

Article Title: Sestrin2 emerges as a regulator of ROS-mediated oxidative stress and apoptosis in the cadmium-induced liver injury of rare minnow (Gobiocypris rarus).
Article Snippet: The total RNA of the liver tissues (n = 2) and cells from each sample was isolated by using TRIZOL reagent (Invitrogen, Thermo Fisher Scientific) according to manufacturer’s instructions. .. First strand cDNA was transcribed from 1 μg of total RNA by Moloney murine leukemia virus (M-MLV) reverse transcriptase (Sangon Biotech, Shanghai, China). .. In brief, cDNA was synthesized by using a SYBR Green PCR master mix (Bimake, Houston, TX, USA) in an ABI7500 real-time PCR instrument (Bio-Rad, Califormia, USA).

Article Title: Parental exposure to environmental cadmium causes developmental defects in the rare minnow (Gobiocypris rarus) embryos: The association between ROS-mediated oxidative stress and developmental dysfunction
Article Snippet: Cadmium is widely distributed in aquaculture systems.. The exposure of parental fish species to cadmium is known to retard of growth and cause death in offspring.. However, the potential mechanisms responsible for how parental cadmium exposure effects embryonic development yet to be elucidated.

Extraction:

Article Title: TPX2 promotes gastric cancer progression and angiogenesis via Wnt/β-catenin signaling
Article Snippet: .. The extraction of total RNA from GC tissues and cells was initially performed using the Trizol reagent (15596018CN, Invitrogen), and cDNA was subsequently generated through reverse transcription with AMV reverse transcriptase (B600020, Sangon Biotech). .. PCR amplification of the cDNA was then conducted using TB Green FAST qPCR Kit (CN830S, TAKARA, Tokyo, Japan).

Generated:

Article Title: TPX2 promotes gastric cancer progression and angiogenesis via Wnt/β-catenin signaling
Article Snippet: .. The extraction of total RNA from GC tissues and cells was initially performed using the Trizol reagent (15596018CN, Invitrogen), and cDNA was subsequently generated through reverse transcription with AMV reverse transcriptase (B600020, Sangon Biotech). .. PCR amplification of the cDNA was then conducted using TB Green FAST qPCR Kit (CN830S, TAKARA, Tokyo, Japan).

Polymerase Chain Reaction:

Article Title: Ciliatoside A Improves Klebsiella pneumoniae-Induced Pneumonia by Modulating Mitochondrial Autophagy via the SIRT1/PINK1/Parkin Pathway.
Article Snippet: Background: Klebsiella pneumoniae (KP)induced pneumonia has a high incidence rate, and current treatment options remain limited.. The efficacy and mechanism of the novel natural compound Ciliatoside A (CA) against KPinduced pneumonia remain

Article Title: Parental exposure to environmental cadmium causes developmental defects in the rare minnow (Gobiocypris rarus) embryos: The association between ROS-mediated oxidative stress and developmental dysfunction
Article Snippet: Cadmium is widely distributed in aquaculture systems.. The exposure of parental fish species to cadmium is known to retard of growth and cause death in offspring.. However, the potential mechanisms responsible for how parental cadmium exposure effects embryonic development yet to be elucidated.

Amplification:

Article Title: Ciliatoside A Improves Klebsiella pneumoniae-Induced Pneumonia by Modulating Mitochondrial Autophagy via the SIRT1/PINK1/Parkin Pathway.
Article Snippet: Background: Klebsiella pneumoniae (KP)induced pneumonia has a high incidence rate, and current treatment options remain limited.. The efficacy and mechanism of the novel natural compound Ciliatoside A (CA) against KPinduced pneumonia remain

cDNA Synthesis:

Article Title: Parental exposure to environmental cadmium causes developmental defects in the rare minnow (Gobiocypris rarus) embryos: The association between ROS-mediated oxidative stress and developmental dysfunction
Article Snippet: Cadmium is widely distributed in aquaculture systems.. The exposure of parental fish species to cadmium is known to retard of growth and cause death in offspring.. However, the potential mechanisms responsible for how parental cadmium exposure effects embryonic development yet to be elucidated.

SYBR Green Assay:

Article Title: Parental exposure to environmental cadmium causes developmental defects in the rare minnow (Gobiocypris rarus) embryos: The association between ROS-mediated oxidative stress and developmental dysfunction
Article Snippet: Cadmium is widely distributed in aquaculture systems.. The exposure of parental fish species to cadmium is known to retard of growth and cause death in offspring.. However, the potential mechanisms responsible for how parental cadmium exposure effects embryonic development yet to be elucidated.

Fluorescence:

Article Title: Parental exposure to environmental cadmium causes developmental defects in the rare minnow (Gobiocypris rarus) embryos: The association between ROS-mediated oxidative stress and developmental dysfunction
Article Snippet: Cadmium is widely distributed in aquaculture systems.. The exposure of parental fish species to cadmium is known to retard of growth and cause death in offspring.. However, the potential mechanisms responsible for how parental cadmium exposure effects embryonic development yet to be elucidated.

Real-time Polymerase Chain Reaction:

Article Title: Parental exposure to environmental cadmium causes developmental defects in the rare minnow (Gobiocypris rarus) embryos: The association between ROS-mediated oxidative stress and developmental dysfunction
Article Snippet: Cadmium is widely distributed in aquaculture systems.. The exposure of parental fish species to cadmium is known to retard of growth and cause death in offspring.. However, the potential mechanisms responsible for how parental cadmium exposure effects embryonic development yet to be elucidated.



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