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Structured Review

Promega amv rt buffer
Amv Rt Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amv+buffer/amv+rt+buffer/pm39425622-401-36-39
Average 90 stars, based on 1 article reviews
amv rt buffer - by Bioz Stars, 2026-10
90/100 stars

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Incubation:

Article Title: The small nucleolar RNA SnoR28 regulates plant growth and development by directing rRNA maturation.
Article Snippet: .. Briefly, 8 lg DNase I-digested total RNA was mixed with 50 FAM-labeled Gm2396-R primer (Supplemental Data Set S4) in 20 lL of AMV RT buffer (Promega) containing 1 mM or 4 lM dNTP and incubated at 65 C for 5 min. Primer extension with AMV reverse transcriptase (Promega) was performed using two aliquots of RNA in parallel in the presence of either 4 lM or 1 mM dNTPs at 42 C for 1 h. A short 25S rRNA sequence (32-nt, Supplemental Data Set S4) was prepared to precisely map the locations of the modifications. ..

Reverse Transcription:

Article Title: The small nucleolar RNA SnoR28 regulates plant growth and development by directing rRNA maturation.
Article Snippet: .. Briefly, 8 lg DNase I-digested total RNA was mixed with 50 FAM-labeled Gm2396-R primer (Supplemental Data Set S4) in 20 lL of AMV RT buffer (Promega) containing 1 mM or 4 lM dNTP and incubated at 65 C for 5 min. Primer extension with AMV reverse transcriptase (Promega) was performed using two aliquots of RNA in parallel in the presence of either 4 lM or 1 mM dNTPs at 42 C for 1 h. A short 25S rRNA sequence (32-nt, Supplemental Data Set S4) was prepared to precisely map the locations of the modifications. ..

Article Title: Trichoderma L-Lysine-α-Oxidase Producer Strain Culture Fluid Inhibits Impatiens Necrotic Spot Virus.
Article Snippet: Medical Faculty, Medical Institute, Russian University of Peoples Friendship, Moscow, Russia.. Address for correspondence: smirip@yandex.ru.. I. P. Smirnova Impatiens necrotic spot virus (INSV) is a phytopathogen, hazardous for agriculture [1].

Article Title: A High-Throughput Screen for Antiproliferative Peptides in Mammalian Cells Identifies Key Transcription Factor Families.
Article Snippet: .. Total RNA was extracted from 106 cells using the Qiagen RNeasy kit. cDNA was produced in the following 20 μL reverse transcriptase reaction (2 μg Total RNA, 2 μL Oligo-dT primer (Promega, 0.5 μg/μL), 4 μL AMV RT Buffer (Promega), 1 μL 10 mM dNTPs (Roche), 0.25 μL RNAsin (Promega), 0.75 μL AMV RT (Promega)). .. Cells were cultured in media with 10 μM BrdU (Invitrogen B23151) for 30 min for K562 cells and 1 h for HeLa cells.

Article Title: Proteomic analysis of CHIKV-infected human fibroblast-like synoviocytes: Identification of host factors potentially associated with CHIKV replication and cellular pathogenesis.
Article Snippet: This article is protected by copyright.. All rights reserved.. A cc ep te d A rt ic le arthralgic fever.

Sequencing:

Article Title: The small nucleolar RNA SnoR28 regulates plant growth and development by directing rRNA maturation.
Article Snippet: .. Briefly, 8 lg DNase I-digested total RNA was mixed with 50 FAM-labeled Gm2396-R primer (Supplemental Data Set S4) in 20 lL of AMV RT buffer (Promega) containing 1 mM or 4 lM dNTP and incubated at 65 C for 5 min. Primer extension with AMV reverse transcriptase (Promega) was performed using two aliquots of RNA in parallel in the presence of either 4 lM or 1 mM dNTPs at 42 C for 1 h. A short 25S rRNA sequence (32-nt, Supplemental Data Set S4) was prepared to precisely map the locations of the modifications. ..

other:

Article Title: Hydrogel-based milliwell arrays for standardized and scalable retinal organoid cultures
Article Snippet: For the reverse transcription reaction, 4 μl AMV-RT buffer (Promega, Madison, USA), 2 μl dNTP mix (Promega, Madison, USA; 10 mM), 1 μl RNAse Inhibitor (RNAsin; Promega, Madison, USA), and 1 μl AMV reverse transcriptase (AMV-RT; Promega, Madison, USA) were added to a 20 μl final volume reaction and incubated at 42 °C for 1 h, followed by 10 min incubation at 95 °C.

Article Title: Cone Genesis Tracing by the Chrnb4 -EGFP Mouse Line: Evidences of Cellular Material Fusion after Cone Precursor Transplantation
Article Snippet: For the reverse transcription reaction, 4 ml AMV-RT buffer (Promega), 2 ml dNTP mix (Promega, 10 mM), 1 ml RNase Inhibitor (RNAsin, Promega), and 1 ml AMV reverse transcriptase (AMV-RT, Promega) were added to a 20 ml final volume reaction and incubated at 42 C for 1 hr, followed by a 10-min incubation at 95 C.

Article Title: Cone Genesis Tracing by the Chrnb4 -EGFP Mouse Line: Evidences of Cellular Material Fusion after Cone Precursor Transplantation
Article Snippet: For the reverse transcription reaction, 4 μl AMV-RT buffer (Promega), 2 μl dNTP mix (Promega, 10 mM), 1 μl RNase Inhibitor (RNAsin, Promega), and 1 μl AMV reverse transcriptase (AMV-RT, Promega) were added to a 20 μl final volume reaction and incubated at 42°C for 1 hr, followed by a 10-min incubation at 95°C.

Produced:

Article Title: A High-Throughput Screen for Antiproliferative Peptides in Mammalian Cells Identifies Key Transcription Factor Families.
Article Snippet: .. Total RNA was extracted from 106 cells using the Qiagen RNeasy kit. cDNA was produced in the following 20 μL reverse transcriptase reaction (2 μg Total RNA, 2 μL Oligo-dT primer (Promega, 0.5 μg/μL), 4 μL AMV RT Buffer (Promega), 1 μL 10 mM dNTPs (Roche), 0.25 μL RNAsin (Promega), 0.75 μL AMV RT (Promega)). .. Cells were cultured in media with 10 μM BrdU (Invitrogen B23151) for 30 min for K562 cells and 1 h for HeLa cells.



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Image Search Results


Journal: Cell reports

Article Title: TRMT1L-catalyzed m 2 2 G27 on tyrosine tRNA is required for efficient mRNA translation and cell survival under oxidative stress

doi: 10.1016/j.celrep.2024.115167

Figure Lengend Snippet:

Article Snippet: AMV Reverse Transcriptase 5X Reaction Buffer , Promega , M515A.

Techniques: Recombinant, Membrane, Reverse Transcription, Protease Inhibitor, Magnetic Beads, Staining, Mutagenesis, Silver Staining, BIA-KA, RNA Sequencing, Plasmid Preparation, Software