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amp1 buffer  (Agdia Inc)

 
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    Agdia Inc amp1 buffer
    Amp1 Buffer, supplied by Agdia Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amp1+buffer/amp1+buffer/pmc11546398-61-27-29
    Average 90 stars, based on 1 article reviews
    amp1 buffer - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: A Field Deployable Real-Time Loop-Mediated Isothermal Amplification Targeting Five Copy nrdB Gene for the Detection of ‘Candidatus Liberibacter asiaticus’ in Citrus
    Article Snippet: Citrus leaf crude extracts were prepared in a sample mesh bag (Agdia, Elkhart, IN, USA) containing 100 mg of finely sliced midribs excised from three leaves and 500 μl of AMP1 extraction buf- fer (Agdia).

    Article Title: Rapid and sensitive isothermal detection of Clavibacter michiganensis subsp. michiganensis using AmplifyRP®
    Article Snippet: Commercial field detection kits for Clavibacter michiganensis subsp. michiganensis (Cmm) have historically been limited to immunoassay-based formats such as ELISA and lateral flow-based technologies.. While effective for high-throughput screening purposes, they are known to cross-react with other Clavibacter michiganensis subspecies, as well as some non-pathogenic bacteria that persist in soil.. The cross-reactivity of these methods mandates confirmation testing of positive results by a laboratory equipped for PCR, media plating, and microscopy detection.

    Extraction:

    Article Title: An Improved Recombinase Polymerase Amplification Coupled with Lateral Flow Assay for Rapid Field Detection of ‘<i>Candidatus</i> Liberibacter asiaticus’
    Article Snippet: Huanglongbing (HLB) is a destructive citrus disease that affects citrus production worldwide.. ‘Candidatus Liberibacter asiaticus’ (CLas), a phloem-limited bacterium, is the associated causal agent of HLB.. The current standard for detection of CLas is real-time quantitative polymerase chain reaction (qPCR) using either the CLas 16S rRNA gene or the ribonucleotide reductase (RNR) gene-specific primers/probe. qPCR requires well-equipped laboratories and trained personnel, which is not convenient for rapid field detection of CLas-infected trees.

    Article Title: Development of a recombinase polymerase based isothermal amplification combined with lateral flow assay (HLB-RPA-LFA) for rapid detection of " Candidatus Liberibacter asiaticus"
    Article Snippet: .. To develop an on-site diagnostic kit, the crude sap was prepared in AMP1 extraction buffer (ACC 00117/0020, Agdia, USA). ..

    Diagnostic Assay:

    Article Title: Development of a recombinase polymerase based isothermal amplification combined with lateral flow assay (HLB-RPA-LFA) for rapid detection of " Candidatus Liberibacter asiaticus"
    Article Snippet: .. To develop an on-site diagnostic kit, the crude sap was prepared in AMP1 extraction buffer (ACC 00117/0020, Agdia, USA). ..



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    amp1 buffer  (Agdia Inc)
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    Agdia Inc amp1 buffer
    Amp1 Buffer, supplied by Agdia Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amp1+buffer/amp1+buffer/pmc11546398-61-27-29
    Average 90 stars, based on 1 article reviews
    amp1 buffer - by Bioz Stars, 2026-11
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    Determination of the detection limit <t>of</t> <t>LAMP-A</t> w assay using a serially diluted recombinant plasmid containing the LAMP target (F3-B3 PCR amplicon). ( A ) Real-time PCR (qPCR) amplification curve conducted with LAMP F3/B3 primer set using a serially diluted recombinant plasmid as a template. ( B ) Standard curve generated with the qPCR results in ( A ). The table on the right shows the average qPCR Ct value of triplicates in ( A ) and the Log 10 copy number of the target molecule of each sample that was calculated using a linear regression equation of the standard curve; y = −3.8721x + 41.161 (R 2 = 0.9978) where y and x denote Ct and the Log 10 target copy number, respectively. ( C ) The amplification curve of the LAMP-A w assay was conducted with the serially diluted recombinant plasmid <t>DNA</t> that was used in ( A ). The relative fluorescence unit (RFU) of the LAMP reaction in ( C ) was measured every 30 s (i.e., one LAMP cycle = 30 s). The sample IDs are indicated in the figure. The threshold lines for qPCR and LAMP-A w are indicated in the amplification graphs in ( A ) and ( C ), respectively.
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    Determination of the detection limit <t>of</t> <t>LAMP-A</t> w assay using a serially diluted recombinant plasmid containing the LAMP target (F3-B3 PCR amplicon). ( A ) Real-time PCR (qPCR) amplification curve conducted with LAMP F3/B3 primer set using a serially diluted recombinant plasmid as a template. ( B ) Standard curve generated with the qPCR results in ( A ). The table on the right shows the average qPCR Ct value of triplicates in ( A ) and the Log 10 copy number of the target molecule of each sample that was calculated using a linear regression equation of the standard curve; y = −3.8721x + 41.161 (R 2 = 0.9978) where y and x denote Ct and the Log 10 target copy number, respectively. ( C ) The amplification curve of the LAMP-A w assay was conducted with the serially diluted recombinant plasmid <t>DNA</t> that was used in ( A ). The relative fluorescence unit (RFU) of the LAMP reaction in ( C ) was measured every 30 s (i.e., one LAMP cycle = 30 s). The sample IDs are indicated in the figure. The threshold lines for qPCR and LAMP-A w are indicated in the amplification graphs in ( A ) and ( C ), respectively.
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    amp1 extraction buffer acc 00117/0020  (Agdia Inc)
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    Determination of the detection limit <t>of</t> <t>LAMP-A</t> w assay using a serially diluted recombinant plasmid containing the LAMP target (F3-B3 PCR amplicon). ( A ) Real-time PCR (qPCR) amplification curve conducted with LAMP F3/B3 primer set using a serially diluted recombinant plasmid as a template. ( B ) Standard curve generated with the qPCR results in ( A ). The table on the right shows the average qPCR Ct value of triplicates in ( A ) and the Log 10 copy number of the target molecule of each sample that was calculated using a linear regression equation of the standard curve; y = −3.8721x + 41.161 (R 2 = 0.9978) where y and x denote Ct and the Log 10 target copy number, respectively. ( C ) The amplification curve of the LAMP-A w assay was conducted with the serially diluted recombinant plasmid <t>DNA</t> that was used in ( A ). The relative fluorescence unit (RFU) of the LAMP reaction in ( C ) was measured every 30 s (i.e., one LAMP cycle = 30 s). The sample IDs are indicated in the figure. The threshold lines for qPCR and LAMP-A w are indicated in the amplification graphs in ( A ) and ( C ), respectively.
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    extraction buffer amp1  (Agdia Inc)
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    Determination of the detection limit <t>of</t> <t>LAMP-A</t> w assay using a serially diluted recombinant plasmid containing the LAMP target (F3-B3 PCR amplicon). ( A ) Real-time PCR (qPCR) amplification curve conducted with LAMP F3/B3 primer set using a serially diluted recombinant plasmid as a template. ( B ) Standard curve generated with the qPCR results in ( A ). The table on the right shows the average qPCR Ct value of triplicates in ( A ) and the Log 10 copy number of the target molecule of each sample that was calculated using a linear regression equation of the standard curve; y = −3.8721x + 41.161 (R 2 = 0.9978) where y and x denote Ct and the Log 10 target copy number, respectively. ( C ) The amplification curve of the LAMP-A w assay was conducted with the serially diluted recombinant plasmid <t>DNA</t> that was used in ( A ). The relative fluorescence unit (RFU) of the LAMP reaction in ( C ) was measured every 30 s (i.e., one LAMP cycle = 30 s). The sample IDs are indicated in the figure. The threshold lines for qPCR and LAMP-A w are indicated in the amplification graphs in ( A ) and ( C ), respectively.
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    Determination of the detection limit of LAMP-A w assay using a serially diluted recombinant plasmid containing the LAMP target (F3-B3 PCR amplicon). ( A ) Real-time PCR (qPCR) amplification curve conducted with LAMP F3/B3 primer set using a serially diluted recombinant plasmid as a template. ( B ) Standard curve generated with the qPCR results in ( A ). The table on the right shows the average qPCR Ct value of triplicates in ( A ) and the Log 10 copy number of the target molecule of each sample that was calculated using a linear regression equation of the standard curve; y = −3.8721x + 41.161 (R 2 = 0.9978) where y and x denote Ct and the Log 10 target copy number, respectively. ( C ) The amplification curve of the LAMP-A w assay was conducted with the serially diluted recombinant plasmid DNA that was used in ( A ). The relative fluorescence unit (RFU) of the LAMP reaction in ( C ) was measured every 30 s (i.e., one LAMP cycle = 30 s). The sample IDs are indicated in the figure. The threshold lines for qPCR and LAMP-A w are indicated in the amplification graphs in ( A ) and ( C ), respectively.

    Journal: International Journal of Molecular Sciences

    Article Title: Loop-Mediated Isothermal Amplification Assay for the Detection of Citrus Canker Causing Bacterial Variant, Xanthomonas citri pv. citri A w Strain

    doi: 10.3390/ijms252111590

    Figure Lengend Snippet: Determination of the detection limit of LAMP-A w assay using a serially diluted recombinant plasmid containing the LAMP target (F3-B3 PCR amplicon). ( A ) Real-time PCR (qPCR) amplification curve conducted with LAMP F3/B3 primer set using a serially diluted recombinant plasmid as a template. ( B ) Standard curve generated with the qPCR results in ( A ). The table on the right shows the average qPCR Ct value of triplicates in ( A ) and the Log 10 copy number of the target molecule of each sample that was calculated using a linear regression equation of the standard curve; y = −3.8721x + 41.161 (R 2 = 0.9978) where y and x denote Ct and the Log 10 target copy number, respectively. ( C ) The amplification curve of the LAMP-A w assay was conducted with the serially diluted recombinant plasmid DNA that was used in ( A ). The relative fluorescence unit (RFU) of the LAMP reaction in ( C ) was measured every 30 s (i.e., one LAMP cycle = 30 s). The sample IDs are indicated in the figure. The threshold lines for qPCR and LAMP-A w are indicated in the amplification graphs in ( A ) and ( C ), respectively.

    Article Snippet: In order to examine the field applicability of the LAMP-A w assay, the LAMP-A w reaction was conducted with crude DNA extracts prepared with 0.8% NaOH or AMP1 buffer (Agdia) following the manufacturer’s instructions, where the sample boiling step is not required for the crude DNA extract preparation.

    Techniques: Recombinant, Plasmid Preparation, Amplification, Real-time Polymerase Chain Reaction, Generated, Fluorescence

    The specificity of the LAMP-A w assay using ( A ) DNA fractions prepared from Candidatus Liberibacter asiaticus (CLas)-positive and canker-negative leaf samples collected in the field and ( B ) two Xcc A positives (Xcc A(+)) and one Xcc A w positive DNA sample (Xcc A w (+)). The non-template control (NTC) is indicated in the figure. The threshold line was indicated in the graph. The RFU of the LAMP-A w reaction was monitored every 30 s (i.e., one LAMP cycle = 30 s).

    Journal: International Journal of Molecular Sciences

    Article Title: Loop-Mediated Isothermal Amplification Assay for the Detection of Citrus Canker Causing Bacterial Variant, Xanthomonas citri pv. citri A w Strain

    doi: 10.3390/ijms252111590

    Figure Lengend Snippet: The specificity of the LAMP-A w assay using ( A ) DNA fractions prepared from Candidatus Liberibacter asiaticus (CLas)-positive and canker-negative leaf samples collected in the field and ( B ) two Xcc A positives (Xcc A(+)) and one Xcc A w positive DNA sample (Xcc A w (+)). The non-template control (NTC) is indicated in the figure. The threshold line was indicated in the graph. The RFU of the LAMP-A w reaction was monitored every 30 s (i.e., one LAMP cycle = 30 s).

    Article Snippet: In order to examine the field applicability of the LAMP-A w assay, the LAMP-A w reaction was conducted with crude DNA extracts prepared with 0.8% NaOH or AMP1 buffer (Agdia) following the manufacturer’s instructions, where the sample boiling step is not required for the crude DNA extract preparation.

    Techniques: Control

    Evaluation of the specificity of LAMP-A w assay coupled with lateral flow immunoassay. Sample IDs are shown on top. Samples 77 and 78 were prepared from Xcc A w -positive leaf samples. Xcc A is the DNA fraction prepared from a Xcc A positive sample. NC (negative control) was the healthy leaf DNA fraction. The ‘water’ sample indicates the LAMP reaction conducted with nuclease-free water. The control and test lines are indicated on the left, which detected the gold conjugate and Biotin- and DIG-labeled LAMP-A w products, respectively.

    Journal: International Journal of Molecular Sciences

    Article Title: Loop-Mediated Isothermal Amplification Assay for the Detection of Citrus Canker Causing Bacterial Variant, Xanthomonas citri pv. citri A w Strain

    doi: 10.3390/ijms252111590

    Figure Lengend Snippet: Evaluation of the specificity of LAMP-A w assay coupled with lateral flow immunoassay. Sample IDs are shown on top. Samples 77 and 78 were prepared from Xcc A w -positive leaf samples. Xcc A is the DNA fraction prepared from a Xcc A positive sample. NC (negative control) was the healthy leaf DNA fraction. The ‘water’ sample indicates the LAMP reaction conducted with nuclease-free water. The control and test lines are indicated on the left, which detected the gold conjugate and Biotin- and DIG-labeled LAMP-A w products, respectively.

    Article Snippet: In order to examine the field applicability of the LAMP-A w assay, the LAMP-A w reaction was conducted with crude DNA extracts prepared with 0.8% NaOH or AMP1 buffer (Agdia) following the manufacturer’s instructions, where the sample boiling step is not required for the crude DNA extract preparation.

    Techniques: Negative Control, Control, Labeling