Software:Article Title: The E3 ligase RNF32 controls the IκB kinase complex and NF-κB signaling in intestinal stem cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER pOPINK-OTUD1 (OTU+UIM, aa 287-481) Mevissen et al. 32 Addgene Cat# 61406 pOPINB-OTULIN (full-length, aa 1-352) Keusekotten et al. 33 Addgene Cat# 61464 Software and Algorithms ImageJ NIH https://imagej.nih.gov/ij/ AlphaFold2-Multimer prediction nf-core pipeline: proteinfold 1.1.0dev (revision c246c853c6) https://nf-co.re/ PyMOL v2.5.0 Schrodinger, LLC http://www.pymol.org GraphPad Prism GraphPad Software https://www.graphpad.com/features Benchling CRISPR Genome Engineering tool Benchling https://www.benchling.com/crispr TomoStudioTM software version X 1.6 Tomocube Inc., Korea N/A Richardson-Lucy deconvolution algorithm using NIS-Elements Advanced Research software Nikon N/A NIS-Elements HC software version 5.20 Nikon N/A Other Advanced DMEM/F12 Gibco Cat# 12634010 GlutaMAXTM Supplement Gibco Cat# 35050061 Penicillin-Streptomycin Solution (×100) Wako Cat# 168-23191 IntesticultTM organoid growth medium (mouse) STEMCELL Technologies Cat# ST-06005 Matrigel Matrix Basement Membrane Growth factor reduced Phenol red free Corning Cat# 356231 Fetal Bovine serum Nichirei Biosciences Inc. Cat# 175012 TrypLETM Express Enzyme (1X), no phenol red Gibco Cat# 12604013 e4 Molecular Cell 85, 1–14.e1–e9, November 20, 2025 .. Where indicated, cells were treated with 10 μM MG132 (PeptaNova GmbH 3175-v), 0.5 μM MLN4924 (Selleck Chemical S7109), 100 μM cycloheximide (Merck C4859), 100 nM thapsigargin (Tocris 1138), 950 nM ionomycin (IO) (Merck I0634), 10 ng/ml phorbol 12-myristate 13-acetate (PMA) (Merck P8139), 20 ng/ml TNFα (Peprotech 300− 01А), 1 μg/ml IL-1β (PeproTech 200-01B), 10 μg/ml LPS (Thermo Fisher Scientific 00-4976-93), 25, 50 or 100 μM W-7 hydrochloride (MedChemExpress), 10 μM calmidazolium (MedChemExpress).
CRISPR:Article Title: The E3 ligase RNF32 controls the IκB kinase complex and NF-κB signaling in intestinal stem cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER pOPINK-OTUD1 (OTU+UIM, aa 287-481) Mevissen et al. 32 Addgene Cat# 61406 pOPINB-OTULIN (full-length, aa 1-352) Keusekotten et al. 33 Addgene Cat# 61464 Software and Algorithms ImageJ NIH https://imagej.nih.gov/ij/ AlphaFold2-Multimer prediction nf-core pipeline: proteinfold 1.1.0dev (revision c246c853c6) https://nf-co.re/ PyMOL v2.5.0 Schrodinger, LLC http://www.pymol.org GraphPad Prism GraphPad Software https://www.graphpad.com/features Benchling CRISPR Genome Engineering tool Benchling https://www.benchling.com/crispr TomoStudioTM software version X 1.6 Tomocube Inc., Korea N/A Richardson-Lucy deconvolution algorithm using NIS-Elements Advanced Research software Nikon N/A NIS-Elements HC software version 5.20 Nikon N/A Other Advanced DMEM/F12 Gibco Cat# 12634010 GlutaMAXTM Supplement Gibco Cat# 35050061 Penicillin-Streptomycin Solution (×100) Wako Cat# 168-23191 IntesticultTM organoid growth medium (mouse) STEMCELL Technologies Cat# ST-06005 Matrigel Matrix Basement Membrane Growth factor reduced Phenol red free Corning Cat# 356231 Fetal Bovine serum Nichirei Biosciences Inc. Cat# 175012 TrypLETM Express Enzyme (1X), no phenol red Gibco Cat# 12604013 e4 Molecular Cell 85, 1–14.e1–e9, November 20, 2025 .. Where indicated, cells were treated with 10 μM MG132 (PeptaNova GmbH 3175-v), 0.5 μM MLN4924 (Selleck Chemical S7109), 100 μM cycloheximide (Merck C4859), 100 nM thapsigargin (Tocris 1138), 950 nM ionomycin (IO) (Merck I0634), 10 ng/ml phorbol 12-myristate 13-acetate (PMA) (Merck P8139), 20 ng/ml TNFα (Peprotech 300− 01А), 1 μg/ml IL-1β (PeproTech 200-01B), 10 μg/ml LPS (Thermo Fisher Scientific 00-4976-93), 25, 50 or 100 μM W-7 hydrochloride (MedChemExpress), 10 μM calmidazolium (MedChemExpress).
Membrane:Article Title: The E3 ligase RNF32 controls the IκB kinase complex and NF-κB signaling in intestinal stem cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER pOPINK-OTUD1 (OTU+UIM, aa 287-481) Mevissen et al. 32 Addgene Cat# 61406 pOPINB-OTULIN (full-length, aa 1-352) Keusekotten et al. 33 Addgene Cat# 61464 Software and Algorithms ImageJ NIH https://imagej.nih.gov/ij/ AlphaFold2-Multimer prediction nf-core pipeline: proteinfold 1.1.0dev (revision c246c853c6) https://nf-co.re/ PyMOL v2.5.0 Schrodinger, LLC http://www.pymol.org GraphPad Prism GraphPad Software https://www.graphpad.com/features Benchling CRISPR Genome Engineering tool Benchling https://www.benchling.com/crispr TomoStudioTM software version X 1.6 Tomocube Inc., Korea N/A Richardson-Lucy deconvolution algorithm using NIS-Elements Advanced Research software Nikon N/A NIS-Elements HC software version 5.20 Nikon N/A Other Advanced DMEM/F12 Gibco Cat# 12634010 GlutaMAXTM Supplement Gibco Cat# 35050061 Penicillin-Streptomycin Solution (×100) Wako Cat# 168-23191 IntesticultTM organoid growth medium (mouse) STEMCELL Technologies Cat# ST-06005 Matrigel Matrix Basement Membrane Growth factor reduced Phenol red free Corning Cat# 356231 Fetal Bovine serum Nichirei Biosciences Inc. Cat# 175012 TrypLETM Express Enzyme (1X), no phenol red Gibco Cat# 12604013 e4 Molecular Cell 85, 1–14.e1–e9, November 20, 2025 .. Where indicated, cells were treated with 10 μM MG132 (PeptaNova GmbH 3175-v), 0.5 μM MLN4924 (Selleck Chemical S7109), 100 μM cycloheximide (Merck C4859), 100 nM thapsigargin (Tocris 1138), 950 nM ionomycin (IO) (Merck I0634), 10 ng/ml phorbol 12-myristate 13-acetate (PMA) (Merck P8139), 20 ng/ml TNFα (Peprotech 300− 01А), 1 μg/ml IL-1β (PeproTech 200-01B), 10 μg/ml LPS (Thermo Fisher Scientific 00-4976-93), 25, 50 or 100 μM W-7 hydrochloride (MedChemExpress), 10 μM calmidazolium (MedChemExpress).
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