software, algorithm prism (GraphPad Software Inc)
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GraphPad Software Inc
software, algorithm prism
Software, Algorithm Prism, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/algorithms+prism+graphpad+software/software++algorithm+prism/pmc09084894-93-5-3
Average 90 stars, based on 1 article reviews
Software, Algorithm Prism, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/algorithms+prism+graphpad+software/software++algorithm+prism/pmc09084894-93-5-3
Average 90 stars, based on 1 article reviews
software, algorithm prism - by Bioz Stars,
2026-11
90/100 stars
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1) Product Images from "MDGAs are fast-diffusing molecules that delay excitatory synapse development by altering neuroligin behavior"
Article Title: MDGAs are fast-diffusing molecules that delay excitatory synapse development by altering neuroligin behavior
Journal: eLife
doi: 10.7554/eLife.75233
Figure Legend Snippet:
Techniques Used: Sequencing, Control, CRISPR, Biomarker Discovery, Reverse Transcription, Expressing, Recombinant, Electroporation, Plasmid Preparation, Blocking Assay, Transmission Assay, RNA Extraction, Lysis, Transfection, Cloning, cDNA Synthesis, Software, Microscopy, Imaging, Western Blot, Quantitation Assay
Related Articles
Software:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Isolation:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Antibody Purification:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Protein Purification:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Western Blot:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Transfection:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Glo Assay:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Bradford Assay:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Reverse Transcription:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and SYBR Green Assay:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Real-time Polymerase Chain Reaction:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Viability Assay:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Recombinant:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Plasmid Preparation:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Imaging:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Virus:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Multiplex Assay:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Fluorescence:Article Title: Early recovery of proteasome activity in cells pulse-treated with proteasome inhibitors is independent of DDI2 Article Snippet: DOI: https://doi.org/10.7554/eLife.91678 9 of 14 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Anti- DDI2 (rabbit pAb) Bethyl Laboratories Cat# A304- 629A WB (1:5000) Antibody Anti- rabbit IgG, HRP- linked (goat) Cell Signaling Cat#7074 WB (1:1000) Antibody Anti- mouse IgG, HRPlinked (goat) Cell Signaling Cat#7076 P2 WB (1:1000) Antibody Goat anti- Rabbit IgG, Alexa Fluor Plus 647 Thermofisher - Invitrogen Cat#A32733 WB (1:3500) Antibody Goat anti- Rabbit IgG, Alexa Fluor 680 Thermofisher - Invitrogen Cat#A- 21076 WB (1:3500) Antibody IRDye 800CW Goat antiMouse IgG LI- COR Cat#926–32210 WB (1:3500) Commercial assay or kit DharmaFECT 1 Horizon Discovery - Dharmacon T- 2001–03 Transfection reagent for MDAMB- 231 and SUM- 149 cells Commercial assay or kit Proteasome- Glo Assay Promega G8622 Assay for Chymotrypsin- like Commercial assay or kit CellTiter- Glo Assay Promega G7572 Assay for Cell Viability Commercial assay or kit Pierce Coomassie Plus (Bradford) Assay ThermoFisher - Life Technologies 23238 Assay for Protein Quantification Commercial assay or kit TRIzol Reagent ThermoFisher - Life Technologies 15596018 RNA Isolation Commercial assay or kit High- Capacity cDNA Reverse Transcription kit Thermofisher - Applied Biosystems 4368814 Commercial assay or kit 2 x SYBR Green Bimake qPCR Master Mix Selleckchem - Bimake B21203 Commercial assay or kit RNasin Plus Ribonuclease Inhibitor Promega N2615 Chemical compound, drug Bortezomib LC Laboratories AS# 179324- 69- 7, Cat# B- 1408 Proteasome Inhibitor, Chemical compound, drug Carfilzomib LC Laboratories CAS# 868540- 17- 4, Cat# C3022 Proteasome Inhibitors, Chemical compound, drug CB- 5083 Cayman Chemicals CAS# 1542705- 92- 9, Cat# 19311 p97 inhibitor, Chemical compound, drug CHAPS (3-((3- cholamidopropyl) dimethylammonio)–1propanesulfonate) Thermo Scientific CAS# 331717- 45- 4, Cat # 28300 Detergent Chemical compound, drug Cycloheximide Sigma- Aldrich CAS# 66- 81- 9, Cat #C1988 Protein Synthesis Inhibitor, Chemical compound, drug Digitonin GoldBio CAS# 11024- 24- 1, Cat# D180–250 Detergent Chemical compound, drug PhosSTOP Roche Cat# 4906837001 Mixture of Phosphatase Inhibitors Chemical compound, drug Suc- LLVY- AMC Bachem CAS# 94367- 21- 2, Cat # 4011369 Proteasome substrate Chemical compound, drug Resazurin sodium salt Sigma- Aldrich CAS# 62758- 13- 8, Cat#R7017 Alamar Blue Viability Assay Software, Article Title: Discovery of ZFD-10 of a pyridazino[4,5-b]indol-4(5H)-one derivative as an anti-ZIKV agent and a ZIKV NS5 RdRp inhibitor. Article Snippet: ll OPEN ACCESS iScience Article Title: A synaptic amplifier of hunger for regaining body weight in the hypothalamus. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies rat monoclonal anti-mCherry Thermofisher M11217 chicken polyclonal anti-GFP Abcam ab13970 rabbit polyclonal anti-c-Fos Synaptic System 226 003 donkey anti-rat Alexa594 Thermofisher A-21209 goat anti-chicken Alexa488 Thermofisher A-11039 donkey anti-rabbit Alexa488 Thermofisher A-21206 Bacterial and Virus Strains pAAV-EF1a-double floxed-hChR2(H134R)mCherry-WPRE-HGHpA Addgene RRID: Addgene_20297 pAAV-EF1a-double floxed-hChR2(H134R)EYFP-WPRE-HGHpA Addgene RRID: Addgene_20298 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Addgene RRID: Addgene_44361 pAAV-hSyn-DIO-mCherry Addgene RRID: Addgene_50459 pAAV-FLEX-hGlyR-mCherry Todd et al. 42 N/A pAAV-FREX-hM3Dq-mCherry This paper N/A pAAV-FREX-Cre This paper N/A Chemicals, peptides, and recombinant proteins Bicuculline Sigma-Aldrich Cat# 14343 Ivermectin Sigma-Aldrich I8898 Clozapine N-oxide (CNO) Hello Bio Cat#HB6149 (+)-MK 801 maleate Hello Bio HB0004 D-AP5 Alomone labs Cat# D-145 CNQX disodium salt Hello Bio HB0205 Critical commercial assays RNAscope Multiplex Fluorescent Detection Kit v2 ACD bio / Bio-Techne Cat# 323 110 RNAscope Target Retrieval Reagents ACD bio / Bio-Techne Cat# 322 000 Protease Plus ACD bio / Bio-Techne Cat# 322 380 TSA PLUS Fluorescence Kits Perkin-Elmer Cat# NEL760001KT Experimental models: Organisms/strains Mouse: B6; 129S-Trhtm1.1(cre)Mjkr/LowlJ The Jackson Laboratory RRID: IMSR_JAX:032468 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: B6.FVB-Tg(Npy-hrGFP)1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:006417 Mouse: Agrptm1(cre)Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012899 Mouse: B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ The Jackson Laboratory RRID: IMSR_JAX:028863 Mouse: Slc17a6tm1Lowl/J The Jackson Laboratory RRID: IMSR_JAX:012898 Mouse: Trh-p2a-Dre This paper N/A Software & algorithms Clampfit Molecular Devices https://www.moleculardevices.com Illustrator CC Adobe Systems https://www.adobe.com/products/illustrator ImageJ Schneider et al.74 https://imagej.nih.gov/ij pCLAMP 10.7 Molecular Devices https://www.moleculardevices.com Article Title: Mapping immunodominant sites on the MERS-CoV spike glycoprotein targeted by infection-elicited antibodies in humans. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1(+) MERS-CoV EMC/2012 S full-length Millet and Whittaker13 N/A pcDNA3.1(+) MERS-CoV United Kingdom/2012 S full-length GenScript N/A pcDNA3.1(+) MERS-CoV Riyadh/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Seoul/2015 S GenScript N/A pcDNA3.1(+) MERS-CoV Kenya/2019 S GenScript N/A pcDNA3.1(+) MjHKU4r-CoV-1 D16 S GenScript N/A pcDNA3.1(+) LCA60 Heavy Chain GenScript N/A pcDNA3.1(+) LCA60 Light Chain GenScript N/A pcDNA3.1(+) S41 Heavy Chain GenScript N/A pcDNA3.1(+) S41 Light Chain GenScript N/A pcDNA3.1(+) 4C2 Heavy Chain GenScript N/A pcDNA3.1(+) 4C2 Light Chain GenScript N/A pcDNA3.1(+) 76E1 Heavy Chain GenScript N/A pcDNA3.1(+) 76E1 Light Chain GenScript N/A pcDNA3.1(+) MERS-CoV NTD GenScript N/A pCMVR MERS-CoV RBD GenScript N/A pcDNA3.4 MERS-CoV S1 GenScript N/A pcDNA3.1( ) DPP4-FC GenScript N/A Software and Article Title: Biallelic variants in MAD2L1BP ( p31 comet ) cause female infertility characterized by oocyte maturation arrest Article Snippet: Software, algorithm , Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and Article Title: Identification of a regulatory pathway governing TRAF1 via an arthritis-associated non-coding variant Article Snippet: This paper NA Recombinant DNA pGL3 promoter vector Promega E1761 pRL-TK vector Promega E2241 Software and Article Title: Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Restriction enzyme for CYP6Pa: Taq I (cut site, 50-TCGA-30) New England Biolabs Cat# R0149S Restriction enzyme for CYP6Pb: NmuCl (Tsp45I) (cut site 50-GTSAC-30) New England Biolabs Cat# R0583S Software and |