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agpase-based pyrosequencing system  (Pyrosequencing Inc)

 
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    Pyrosequencing Inc agpase-based pyrosequencing system
    Agpase Based Pyrosequencing System, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/agpase-based+pyrosequencing/agpase+based+pyrosequencing+system/us09790547-281-4-5
    Average 90 stars, based on 1 article reviews
    agpase-based pyrosequencing system - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Comparison:

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: However, besides the decreasing signal intensity, 75 bases can be successfully sequenced and analyzed using the AGPase-based pyrosequencing system according to an embodiment of the invention with about 98.7% base call accuracy as is illustrated in FIG. 6.

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: The allelic frequency determination on mixtures of two different PCR products was carried out using the AGPase-based pyrosequencing system according to an embodiment of the present invention.

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: A Comparison Between the AGPase-based Pyrosequencing System, the PPDK-based Pyrosequencing System, and the Conventional ATP Sulfurylase-based Pyrosequencing System In order to compare these three systems at the same conditions, all the components in the reaction mixtures were kept identical except for using APS and ATP sulfurylase in the conventional sequencing reaction, AMP, PEP and PPDK (cloned, expressed and purified with 15 U/mg protein activity, FIG. 8) in the PPDK-based reaction mixture, and ADPGlc and AGPase were used in the pyrosequencing mixture according to an embodiment of the present invention.

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: The second reaction in the AGPase-based pyrosequencing technology according to an embodiment of the present invention, namely the production of ATP from PPi released upon DNA polymerization, is catalyzed by AGPase: PPi+ADP-GlcATP +Glc-1-phosphate.

    Sequencing:

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: However, besides the decreasing signal intensity, 75 bases can be successfully sequenced and analyzed using the AGPase-based pyrosequencing system according to an embodiment of the invention with about 98.7% base call accuracy as is illustrated in FIG. 6.

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: The allelic frequency determination on mixtures of two different PCR products was carried out using the AGPase-based pyrosequencing system according to an embodiment of the present invention.

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: A Comparison Between the AGPase-based Pyrosequencing System, the PPDK-based Pyrosequencing System, and the Conventional ATP Sulfurylase-based Pyrosequencing System In order to compare these three systems at the same conditions, all the components in the reaction mixtures were kept identical except for using APS and ATP sulfurylase in the conventional sequencing reaction, AMP, PEP and PPDK (cloned, expressed and purified with 15 U/mg protein activity, FIG. 8) in the PPDK-based reaction mixture, and ADPGlc and AGPase were used in the pyrosequencing mixture according to an embodiment of the present invention.

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: The second reaction in the AGPase-based pyrosequencing technology according to an embodiment of the present invention, namely the production of ATP from PPi released upon DNA polymerization, is catalyzed by AGPase: PPi+ADP-GlcATP +Glc-1-phosphate.

    Purification:

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: However, besides the decreasing signal intensity, 75 bases can be successfully sequenced and analyzed using the AGPase-based pyrosequencing system according to an embodiment of the invention with about 98.7% base call accuracy as is illustrated in FIG. 6.

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: The allelic frequency determination on mixtures of two different PCR products was carried out using the AGPase-based pyrosequencing system according to an embodiment of the present invention.

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: A Comparison Between the AGPase-based Pyrosequencing System, the PPDK-based Pyrosequencing System, and the Conventional ATP Sulfurylase-based Pyrosequencing System In order to compare these three systems at the same conditions, all the components in the reaction mixtures were kept identical except for using APS and ATP sulfurylase in the conventional sequencing reaction, AMP, PEP and PPDK (cloned, expressed and purified with 15 U/mg protein activity, FIG. 8) in the PPDK-based reaction mixture, and ADPGlc and AGPase were used in the pyrosequencing mixture according to an embodiment of the present invention.

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: The second reaction in the AGPase-based pyrosequencing technology according to an embodiment of the present invention, namely the production of ATP from PPi released upon DNA polymerization, is catalyzed by AGPase: PPi+ADP-GlcATP +Glc-1-phosphate.

    Activity Assay:

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: However, besides the decreasing signal intensity, 75 bases can be successfully sequenced and analyzed using the AGPase-based pyrosequencing system according to an embodiment of the invention with about 98.7% base call accuracy as is illustrated in FIG. 6.

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: The allelic frequency determination on mixtures of two different PCR products was carried out using the AGPase-based pyrosequencing system according to an embodiment of the present invention.

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: A Comparison Between the AGPase-based Pyrosequencing System, the PPDK-based Pyrosequencing System, and the Conventional ATP Sulfurylase-based Pyrosequencing System In order to compare these three systems at the same conditions, all the components in the reaction mixtures were kept identical except for using APS and ATP sulfurylase in the conventional sequencing reaction, AMP, PEP and PPDK (cloned, expressed and purified with 15 U/mg protein activity, FIG. 8) in the PPDK-based reaction mixture, and ADPGlc and AGPase were used in the pyrosequencing mixture according to an embodiment of the present invention.

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: The second reaction in the AGPase-based pyrosequencing technology according to an embodiment of the present invention, namely the production of ATP from PPi released upon DNA polymerization, is catalyzed by AGPase: PPi+ADP-GlcATP +Glc-1-phosphate.

    Polymerase Chain Reaction:

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: However, besides the decreasing signal intensity, 75 bases can be successfully sequenced and analyzed using the AGPase-based pyrosequencing system according to an embodiment of the invention with about 98.7% base call accuracy as is illustrated in FIG. 6.

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: The allelic frequency determination on mixtures of two different PCR products was carried out using the AGPase-based pyrosequencing system according to an embodiment of the present invention.

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: A Comparison Between the AGPase-based Pyrosequencing System, the PPDK-based Pyrosequencing System, and the Conventional ATP Sulfurylase-based Pyrosequencing System In order to compare these three systems at the same conditions, all the components in the reaction mixtures were kept identical except for using APS and ATP sulfurylase in the conventional sequencing reaction, AMP, PEP and PPDK (cloned, expressed and purified with 15 U/mg protein activity, FIG. 8) in the PPDK-based reaction mixture, and ADPGlc and AGPase were used in the pyrosequencing mixture according to an embodiment of the present invention.

    Article Title: Integrated microfluidic and solid state pyrosequencing systems
    Article Snippet: The second reaction in the AGPase-based pyrosequencing technology according to an embodiment of the present invention, namely the production of ATP from PPi released upon DNA polymerization, is catalyzed by AGPase: PPi+ADP-GlcATP +Glc-1-phosphate.



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