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Chemunex Inc lysis buffer aex
Lysis Buffer Aex, supplied by Chemunex Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aex+buffer/lysis+buffer+aex/pmc03433903-104-35-38
Average 90 stars, based on 1 article reviews
lysis buffer aex - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Incubation:

Article Title: Genetic Mechanisms Underlying the Pathogenicity of Cold-Stressed Salmonella enterica Serovar Typhimurium in Cultured Intestinal Epithelial Cells
Article Snippet: .. Upon incubation, medium was aspirated, cells were washed three times with 200 μl standard Tyrode's buffer and then lysed using 50 μl lysis buffer (AEX Chemunex, France) as described previously ( 9 ), and the cell lysate was used to quantify the number of Caco-2 cells and associated bacteria. .. Quantitative bacterial analysis was done using quantitative PCR (qPCR) with a CFX 96 real-time system (Bio-Rad, Hercules, CA).

Article Title: The Yin and Yang of pathogens and probiotics: interplay between Salmonella enterica sv. Typhimurium and Bifidobacterium infantis during co-infection
Article Snippet: .. DNA extraction buffer (AEX Chemunex, France) (50 μL) was used to lyse the monolayer and the bacteria associated with the host, and incubated at 37°C for 15 min followed by 95°C for 15 min. ..

Article Title: Whole Cell Cross-Linking to Discover Host–Microbe Protein Cognate Receptor/Ligand Pairs
Article Snippet: Intracellular bacteria were further quantified by a 2 h incubation in 40 μg/ml gentamicin solution (Sigma-Aldrich, St. Louis, MO, United States) and washed thrice with 200 μl of Tyrodes buffer to remove dead bacterial cells from the surface of the monolayer ( ; ; ; ). .. DNA extraction buffer (AEX Chemunex, France; 50 μl) was used to lyse the monolayer and the bacteria associated with the host, and incubated at 37°C for 15 min followed by 95°C for 15 min. ..

Lysis:

Article Title: Genetic Mechanisms Underlying the Pathogenicity of Cold-Stressed Salmonella enterica Serovar Typhimurium in Cultured Intestinal Epithelial Cells
Article Snippet: .. Upon incubation, medium was aspirated, cells were washed three times with 200 μl standard Tyrode's buffer and then lysed using 50 μl lysis buffer (AEX Chemunex, France) as described previously ( 9 ), and the cell lysate was used to quantify the number of Caco-2 cells and associated bacteria. .. Quantitative bacterial analysis was done using quantitative PCR (qPCR) with a CFX 96 real-time system (Bio-Rad, Hercules, CA).

Article Title: Glycosylation of Human Milk Lactoferrin Exhibits Dynamic Changes During Early Lactation Enhancing Its Role in Pathogenic Bacteria-Host Interactions
Article Snippet: .. Adhered and invaded bacterial counts were performed as described by Elsinghorst ( 42 ), except qPCR was used to determine the bacterial count, and that DNA extraction was performed using 50 μl of a commercial lysis buffer (AEX Chemunex, France) as described by Desai ( 43 ). .. Quantitative bacterial analysis was performed using qPCR with a CFX 96 Real Time System (BioRad, Hercules, CA).

Article Title: Glycosylation of Human Milk Lactoferrin Exhibits Dynamic Changes During Early Lactation Enhancing Its Role in Pathogenic Bacteria-Host Interactions
Article Snippet: .. Adhered and invaded bacterial counts were performed as described by Elsinghorst (42), except qPCR was used to determine the bacterial count, and that DNA extraction was performed using 50 l of a commercial lysis buffer (AEX Chemunex, France) as described by Desai (43). .. Quantitative bacterial analysis was performed using qPCR with a CFX 96 Real Time System (BioRad, Hercules, CA).

Bacteria:

Article Title: Genetic Mechanisms Underlying the Pathogenicity of Cold-Stressed Salmonella enterica Serovar Typhimurium in Cultured Intestinal Epithelial Cells
Article Snippet: .. Upon incubation, medium was aspirated, cells were washed three times with 200 μl standard Tyrode's buffer and then lysed using 50 μl lysis buffer (AEX Chemunex, France) as described previously ( 9 ), and the cell lysate was used to quantify the number of Caco-2 cells and associated bacteria. .. Quantitative bacterial analysis was done using quantitative PCR (qPCR) with a CFX 96 real-time system (Bio-Rad, Hercules, CA).

Article Title: The Yin and Yang of pathogens and probiotics: interplay between Salmonella enterica sv. Typhimurium and Bifidobacterium infantis during co-infection
Article Snippet: .. DNA extraction buffer (AEX Chemunex, France) (50 μL) was used to lyse the monolayer and the bacteria associated with the host, and incubated at 37°C for 15 min followed by 95°C for 15 min. ..

Article Title: Whole Cell Cross-Linking to Discover Host–Microbe Protein Cognate Receptor/Ligand Pairs
Article Snippet: Intracellular bacteria were further quantified by a 2 h incubation in 40 μg/ml gentamicin solution (Sigma-Aldrich, St. Louis, MO, United States) and washed thrice with 200 μl of Tyrodes buffer to remove dead bacterial cells from the surface of the monolayer ( ; ; ; ). .. DNA extraction buffer (AEX Chemunex, France; 50 μl) was used to lyse the monolayer and the bacteria associated with the host, and incubated at 37°C for 15 min followed by 95°C for 15 min. ..

Real-time Polymerase Chain Reaction:

Article Title: Glycosylation of Human Milk Lactoferrin Exhibits Dynamic Changes During Early Lactation Enhancing Its Role in Pathogenic Bacteria-Host Interactions
Article Snippet: .. Adhered and invaded bacterial counts were performed as described by Elsinghorst ( 42 ), except qPCR was used to determine the bacterial count, and that DNA extraction was performed using 50 μl of a commercial lysis buffer (AEX Chemunex, France) as described by Desai ( 43 ). .. Quantitative bacterial analysis was performed using qPCR with a CFX 96 Real Time System (BioRad, Hercules, CA).

Article Title: Glycosylation of Human Milk Lactoferrin Exhibits Dynamic Changes During Early Lactation Enhancing Its Role in Pathogenic Bacteria-Host Interactions
Article Snippet: .. Adhered and invaded bacterial counts were performed as described by Elsinghorst (42), except qPCR was used to determine the bacterial count, and that DNA extraction was performed using 50 l of a commercial lysis buffer (AEX Chemunex, France) as described by Desai (43). .. Quantitative bacterial analysis was performed using qPCR with a CFX 96 Real Time System (BioRad, Hercules, CA).

DNA Extraction:

Article Title: Glycosylation of Human Milk Lactoferrin Exhibits Dynamic Changes During Early Lactation Enhancing Its Role in Pathogenic Bacteria-Host Interactions
Article Snippet: .. Adhered and invaded bacterial counts were performed as described by Elsinghorst ( 42 ), except qPCR was used to determine the bacterial count, and that DNA extraction was performed using 50 μl of a commercial lysis buffer (AEX Chemunex, France) as described by Desai ( 43 ). .. Quantitative bacterial analysis was performed using qPCR with a CFX 96 Real Time System (BioRad, Hercules, CA).

Article Title: The Yin and Yang of pathogens and probiotics: interplay between Salmonella enterica sv. Typhimurium and Bifidobacterium infantis during co-infection
Article Snippet: .. DNA extraction buffer (AEX Chemunex, France) (50 μL) was used to lyse the monolayer and the bacteria associated with the host, and incubated at 37°C for 15 min followed by 95°C for 15 min. ..

Article Title: Whole Cell Cross-Linking to Discover Host–Microbe Protein Cognate Receptor/Ligand Pairs
Article Snippet: Intracellular bacteria were further quantified by a 2 h incubation in 40 μg/ml gentamicin solution (Sigma-Aldrich, St. Louis, MO, United States) and washed thrice with 200 μl of Tyrodes buffer to remove dead bacterial cells from the surface of the monolayer ( ; ; ; ). .. DNA extraction buffer (AEX Chemunex, France; 50 μl) was used to lyse the monolayer and the bacteria associated with the host, and incubated at 37°C for 15 min followed by 95°C for 15 min. ..

Article Title: Glycosylation of Human Milk Lactoferrin Exhibits Dynamic Changes During Early Lactation Enhancing Its Role in Pathogenic Bacteria-Host Interactions
Article Snippet: .. Adhered and invaded bacterial counts were performed as described by Elsinghorst (42), except qPCR was used to determine the bacterial count, and that DNA extraction was performed using 50 l of a commercial lysis buffer (AEX Chemunex, France) as described by Desai (43). .. Quantitative bacterial analysis was performed using qPCR with a CFX 96 Real Time System (BioRad, Hercules, CA).

other:

Article Title: Genetic Mechanisms Underlying the Pathogenicity of Cold-Stressed Salmonella enterica Serovar Typhimurium in Cultured Intestinal Epithelial Cells
Article Snippet: Upon incubation, medium was aspirated, cells were washed three times with 200 l standard Tyrode’s buffer and then lysed using 50 l lysis buffer (AEX Chemunex, France) as described previously (9), and the cell lysate was used to quantify the number of Caco-2 cells and associated bacteria.



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