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ScyTek Inc aec substrate/chromogen kit
Aec Substrate/Chromogen Kit, supplied by ScyTek Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aec+chromagen+kit/aec/pmc07136102-68-0-3
Average 90 stars, based on 1 article reviews
aec substrate/chromogen kit - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Incubation:

Article Title: Rictor deficiency in dendritic cells exacerbates acute kidney injury.
Article Snippet: They were stained with primary rabbit anti-mouse CD11b Ab (1:5000; clone ERR1344, Abcam, Cambridge, MA) overnight at room temperature (RT). .. After washing with PBS, biotinylated goat anti-rabbit secondary Ab (Vector Laboratories; 1:200) was added in combination with a Vectastain ABC kit (Vector Laboratories) at RT for 30 min. After washing, the sections were incubated with AEC (ScyTek ACE500) for 5 min, washed, counterstained with hematoxylin, dehydrated and covered. .. For Ly6B.2 staining, sections were incubated with primary rat anti-mouse Ly6B.2 Ab (1:100; clone 7/4, Bio-Rad, Oxford) overnight at RT, then with secondary biotinylated horse anti-rat Ab (1:200; Vector Laboratories) at RT for 30 min. All other materials and procedures were as for CD11b staining.

Staining:

Article Title: Modulation of PD‑L1 expression by standard therapy in head and neck cancer cell lines and exosomes.
Article Snippet: A secondary biotinylated multilink secondary anti‐rabbit anti‐ body (cat. no. RPN 1004V; Cytiva; 1:200) served for protein detection. .. This step was followed by the application of H2O2 (7%) for 7 min, then Streptavidin‐Biotinylated HRP Complex (Cytiva) was added for 45 min. For staining AEC (ScyTek Biotech Life Sciences) was used. ..

Article Title: Modulation of PD-L1 expression by standard therapy in head and neck cancer cell lines and exosomes
Article Snippet: A secondary biotinylated multilink secondary anti-rabbit antibody (cat. no. RPN 1004V; Cytiva; 1:200) served for protein detection. .. This step was followed by the application of H 2 O 2 (7%) for 7 min, then Streptavidin-Biotinylated HRP Complex (Cytiva) was added for 45 min. For staining AEC (ScyTek Biotech Life Sciences) was used. ..

Article Title: Deficiency of Dol-P-Man Synthase Subunit DPM3 Bridges the Congenital Disorders of Glycosylation with the Dystroglycanopathies
Article Snippet: Anti-spectrin, anti-merosin (laminin-alpha2) and anti-β-dystroglycan (all from Novocastra Laboratories Ltd, UK), and IIH6 (gift from K. Campbell) were used as primary antibodies; Power Vision (Goat a-Mouse/Rb HRP one component, Immunologic, Duiven) as was used as a secondary antibody. .. Slides were stained with AEC (ScyTek Laboratories) for detection. ..

other:

Article Title: Rabbit Annulus Fibrosus Cells Express Neuropeptide Y, Which Is Influenced by Mechanical and Inflammatory Stress
Article Snippet: AEC substrate/chromogen Kit (ScyTek Laboratoties, Inc., Logan, UT, USA) was used for 10 minutes and rinsed in running water for 5 minutes.

Modification:

Article Title: Effects of 1,25-Dihydroxy vitamin D 3 on TNF-α induced inflammation in human chondrocytes and SW1353 cells: a possible role for toll-like receptors.
Article Snippet: The purpose of this study is to evaluate anti-inflammatory and chondro-protective effects of 1,25(OH)2D3 in human chondrocytes and SW1353 cells via investigating expressions of MMPs, TIMPs, VDR, and intracellular signalling pathway mediators such as TLR-2 and -4.. The HC and SW1353 cells were treated with 1,25(OH)2D3 at 10, 100, and 1000 nM concentrations in the absence/presence of TNF-α (20 ng/mL) for 48 h. The mRNA expressions of MMP-1, -2, -3, -9, and -13, TIMP-1 and -2, VDR, TLR-2 and -4 in HC and SW1353 cells were detected by qPCR after treatments.. The cytotoxicity and cell proliferation analyses were assessed by LDH and WST-1 assay, respectively.

Enzyme-linked Immunosorbent Assay:

Article Title: Effects of 1,25-Dihydroxy vitamin D 3 on TNF-α induced inflammation in human chondrocytes and SW1353 cells: a possible role for toll-like receptors.
Article Snippet: The purpose of this study is to evaluate anti-inflammatory and chondro-protective effects of 1,25(OH)2D3 in human chondrocytes and SW1353 cells via investigating expressions of MMPs, TIMPs, VDR, and intracellular signalling pathway mediators such as TLR-2 and -4.. The HC and SW1353 cells were treated with 1,25(OH)2D3 at 10, 100, and 1000 nM concentrations in the absence/presence of TNF-α (20 ng/mL) for 48 h. The mRNA expressions of MMP-1, -2, -3, -9, and -13, TIMP-1 and -2, VDR, TLR-2 and -4 in HC and SW1353 cells were detected by qPCR after treatments.. The cytotoxicity and cell proliferation analyses were assessed by LDH and WST-1 assay, respectively.

Blocking Assay:

Article Title: Effects of 1,25-Dihydroxy vitamin D 3 on TNF-α induced inflammation in human chondrocytes and SW1353 cells: a possible role for toll-like receptors.
Article Snippet: The purpose of this study is to evaluate anti-inflammatory and chondro-protective effects of 1,25(OH)2D3 in human chondrocytes and SW1353 cells via investigating expressions of MMPs, TIMPs, VDR, and intracellular signalling pathway mediators such as TLR-2 and -4.. The HC and SW1353 cells were treated with 1,25(OH)2D3 at 10, 100, and 1000 nM concentrations in the absence/presence of TNF-α (20 ng/mL) for 48 h. The mRNA expressions of MMP-1, -2, -3, -9, and -13, TIMP-1 and -2, VDR, TLR-2 and -4 in HC and SW1353 cells were detected by qPCR after treatments.. The cytotoxicity and cell proliferation analyses were assessed by LDH and WST-1 assay, respectively.

Polymerase Chain Reaction:

Article Title: Effects of 1,25-Dihydroxy vitamin D 3 on TNF-α induced inflammation in human chondrocytes and SW1353 cells: a possible role for toll-like receptors.
Article Snippet: The purpose of this study is to evaluate anti-inflammatory and chondro-protective effects of 1,25(OH)2D3 in human chondrocytes and SW1353 cells via investigating expressions of MMPs, TIMPs, VDR, and intracellular signalling pathway mediators such as TLR-2 and -4.. The HC and SW1353 cells were treated with 1,25(OH)2D3 at 10, 100, and 1000 nM concentrations in the absence/presence of TNF-α (20 ng/mL) for 48 h. The mRNA expressions of MMP-1, -2, -3, -9, and -13, TIMP-1 and -2, VDR, TLR-2 and -4 in HC and SW1353 cells were detected by qPCR after treatments.. The cytotoxicity and cell proliferation analyses were assessed by LDH and WST-1 assay, respectively.

Lysis:

Article Title: Effects of 1,25-Dihydroxy vitamin D 3 on TNF-α induced inflammation in human chondrocytes and SW1353 cells: a possible role for toll-like receptors.
Article Snippet: The purpose of this study is to evaluate anti-inflammatory and chondro-protective effects of 1,25(OH)2D3 in human chondrocytes and SW1353 cells via investigating expressions of MMPs, TIMPs, VDR, and intracellular signalling pathway mediators such as TLR-2 and -4.. The HC and SW1353 cells were treated with 1,25(OH)2D3 at 10, 100, and 1000 nM concentrations in the absence/presence of TNF-α (20 ng/mL) for 48 h. The mRNA expressions of MMP-1, -2, -3, -9, and -13, TIMP-1 and -2, VDR, TLR-2 and -4 in HC and SW1353 cells were detected by qPCR after treatments.. The cytotoxicity and cell proliferation analyses were assessed by LDH and WST-1 assay, respectively.



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