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Becton Dickinson aec solution
Aec Solution, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aec+buffer+a/aec+solution/pmc07145397-176-25-27
Average 90 stars, based on 1 article reviews
aec solution - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Saline:

Article Title: PD1 blockade reverses the suppression of melanoma antigen-specific CTL by CD4 + CD25 Hi regulatory T cells
Article Snippet: After six washings with PBS + 0.05% Tween 20, 1 μg ml −1 of biotinylated anti-human IFN-γ detection mAb (7-B6-1-1 biotin, Mabtech) was added and incubated for 2 h at 37°C. .. The plates then were washed another four times in phosphate buffered saline + Tween 20 and incubated with streptavidin–HRP solution at room temperature for 1 h. Then after 2× plate wash, add AEC solution (BD Bioscience) to detect wells for 4 min. After rinsing with water, the resulting spots were counted on an ELISPOT Reader (Zeiss Inc., Munich, Germany). ..

Incubation:

Article Title: PD1 blockade reverses the suppression of melanoma antigen-specific CTL by CD4 + CD25 Hi regulatory T cells
Article Snippet: After six washings with PBS + 0.05% Tween 20, 1 μg ml −1 of biotinylated anti-human IFN-γ detection mAb (7-B6-1-1 biotin, Mabtech) was added and incubated for 2 h at 37°C. .. The plates then were washed another four times in phosphate buffered saline + Tween 20 and incubated with streptavidin–HRP solution at room temperature for 1 h. Then after 2× plate wash, add AEC solution (BD Bioscience) to detect wells for 4 min. After rinsing with water, the resulting spots were counted on an ELISPOT Reader (Zeiss Inc., Munich, Germany). ..

Article Title: Immunodomination of Serotype-Specific CD4+ T-Cell Epitopes Contributed to the Biased Immune Responses Induced by a Tetravalent Measles-Vectored Dengue Vaccine
Article Snippet: .. After the plates were washed, HRP-conjugated avidin (Research Laboratory Inc.) was added and incubated for 1 h at 37°C, and the assays were developed with AEC solution (BD Pharmingen). .. The reaction was stopped with tap water, and the plates were analyzed using an ImmunoSpot reader with ImmunoSpot software, version 5.0.3 (CTL, Cleveland, OH).

Enzyme-linked Immunospot:

Article Title: PD1 blockade reverses the suppression of melanoma antigen-specific CTL by CD4 + CD25 Hi regulatory T cells
Article Snippet: After six washings with PBS + 0.05% Tween 20, 1 μg ml −1 of biotinylated anti-human IFN-γ detection mAb (7-B6-1-1 biotin, Mabtech) was added and incubated for 2 h at 37°C. .. The plates then were washed another four times in phosphate buffered saline + Tween 20 and incubated with streptavidin–HRP solution at room temperature for 1 h. Then after 2× plate wash, add AEC solution (BD Bioscience) to detect wells for 4 min. After rinsing with water, the resulting spots were counted on an ELISPOT Reader (Zeiss Inc., Munich, Germany). ..

Avidin-Biotin Assay:

Article Title: Immunodomination of Serotype-Specific CD4+ T-Cell Epitopes Contributed to the Biased Immune Responses Induced by a Tetravalent Measles-Vectored Dengue Vaccine
Article Snippet: .. After the plates were washed, HRP-conjugated avidin (Research Laboratory Inc.) was added and incubated for 1 h at 37°C, and the assays were developed with AEC solution (BD Pharmingen). .. The reaction was stopped with tap water, and the plates were analyzed using an ImmunoSpot reader with ImmunoSpot software, version 5.0.3 (CTL, Cleveland, OH).



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