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hepes buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hepes buffer
    Hepes Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ae+buffer/HEPES%2C+0%2E5M+buffer+soln%2E%2C+pH+7%2E6/pmc12241641-62-15-17
    Average 97 stars, based on 1 article reviews
    hepes buffer - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Prevention and treatment of effects of aging and age-associated disorders with ketone supplementation
    Article Snippet: .. VM-M3 cells were cultured in D-glucose, L-glutamine, and sodium pyruvate-free Dulbecco's Modified Eagle Medium (Gibco, Life Technologies) supplemented with 10% fetal bovine serum (Invitrogen), 25 mM D-glucose (Fisher Scientific), 2 mM L-glutamine (Gibco, Life Technologies), 1% penicillin-streptomycin (Invitrogen), and 10 mM HEPES buffer (Gibco, Life Technologies). ..

    Article Title: Conserved T cell receptor usage underpins recognition of CD1c presenting a mycobacterial lipid
    Article Snippet: .. Magnetically-enriched cells were cultured overnight in complete media (RPMI-1640 supplemented with 10% fetal bovine serum (FBS) (JRH Biosciences), penicillin (100 U/ml), streptomycin (100 μg/ml), GlutaMAX (2 mM), sodium pyruvate (1 mM), nonessential amino acids (0.1 mM), Hepes buffer (15 mM) (pH 7.2 to 7.5) (Invitrogen, Life Technologies), and 2 mercaptoethanol (50 μM, Sigma-Aldrich)) supplemented with 50 U/mL recombinant human IL-2 (Peprotech). .. Cells were then harvested, stained as above and FACS-Sorted using an ARIA III (Becton Dickinson).

    Modification:

    Article Title: Prevention and treatment of effects of aging and age-associated disorders with ketone supplementation
    Article Snippet: .. VM-M3 cells were cultured in D-glucose, L-glutamine, and sodium pyruvate-free Dulbecco's Modified Eagle Medium (Gibco, Life Technologies) supplemented with 10% fetal bovine serum (Invitrogen), 25 mM D-glucose (Fisher Scientific), 2 mM L-glutamine (Gibco, Life Technologies), 1% penicillin-streptomycin (Invitrogen), and 10 mM HEPES buffer (Gibco, Life Technologies). ..

    Incubation:

    Article Title: In silico prioritization and cheminformatics identify structurally diverse small-molecule inhibitors of Lassa virus glycoprotein-mediated membrane fusion.
    Article Snippet: CC50 values were calculated in GraphPad Prism using four-parameter nonlinear regression, normalized to DMSO-treated control wells. .. Media was replaced with DMEM containing rVSV-LASVGP-GFP at an MOI 0.1 After 1 h at 37°C, inoculum was aspirated and replaced with DMEM supplemented with 2% FBS and 50 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) (Gibco, Billings, MT) and incubated at 34°C. ..

    Recombinant:

    Article Title: Conserved T cell receptor usage underpins recognition of CD1c presenting a mycobacterial lipid
    Article Snippet: .. Magnetically-enriched cells were cultured overnight in complete media (RPMI-1640 supplemented with 10% fetal bovine serum (FBS) (JRH Biosciences), penicillin (100 U/ml), streptomycin (100 μg/ml), GlutaMAX (2 mM), sodium pyruvate (1 mM), nonessential amino acids (0.1 mM), Hepes buffer (15 mM) (pH 7.2 to 7.5) (Invitrogen, Life Technologies), and 2 mercaptoethanol (50 μM, Sigma-Aldrich)) supplemented with 50 U/mL recombinant human IL-2 (Peprotech). .. Cells were then harvested, stained as above and FACS-Sorted using an ARIA III (Becton Dickinson).

    Concentration Assay:

    Article Title: Biomimetic Nanogels Programmed for Irreversible-Electroporation-Primed Tumor Microenvironments to Elicit Durable Antitumor Immunity
    Article Snippet: .. The dried lipid film was hydrated at 37 °C for 1 h with 20 mM HEPES buffer (Thermo Fisher Scientific, 15630-056) at a total lipid concentration of 2 mg/ml. .. Finally, the liposome suspension was extruded 11 times through a 200-nm polycarbonate membrane (Avanti Polar Lipids) to obtain uniformly sized vesicles.

    Purification:

    Article Title: Inherent tissue homeostasis of the juvenile metaphysis provides a foundation for osteosarcoma development.
    Article Snippet: All enzymes were purchased from New England Biolabs. .. Following overnight digestion at 37 °C, the DNA was purified by ethanol precipitation, and the resulting pellets were resuspended in 100 μL of 25 mM HEPES buffer (Gibco). .. V6.5 ESCs were dissociated using 0.25% trypsin (Nacalai Tesque) and resuspended in 500 μL of high- glucose DMEM (Nacalai Tesque) supplemented with 25 mM HEPES 55.

    Ethanol Precipitation:

    Article Title: Inherent tissue homeostasis of the juvenile metaphysis provides a foundation for osteosarcoma development.
    Article Snippet: All enzymes were purchased from New England Biolabs. .. Following overnight digestion at 37 °C, the DNA was purified by ethanol precipitation, and the resulting pellets were resuspended in 100 μL of 25 mM HEPES buffer (Gibco). .. V6.5 ESCs were dissociated using 0.25% trypsin (Nacalai Tesque) and resuspended in 500 μL of high- glucose DMEM (Nacalai Tesque) supplemented with 25 mM HEPES 55.



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