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sspi digested pet his6 sumo tev lic  (Addgene inc)


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    Structured Review

    Addgene inc sspi digested pet his6 sumo tev lic
    Sspi Digested Pet His6 Sumo Tev Lic, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+vector/pET+His6+Sumo+TEV+LIC+cloning+vector+(1S)+(Plasmid+%2329659)/pmc12591035-252-8-11
    Average 93 stars, based on 56 article reviews
    sspi digested pet his6 sumo tev lic - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Bni5 regulates and coordinates septin architecture and myosin-II functions at the cell division site.
    Article Snippet: .. The PCR products were subcloned into BamHI- and SspI-digested pET-His6Sumo-TEV-LIC (#29659; Addgene) using In-Fusion HD cloning kit (Takara). ..

    Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons.
    Article Snippet: The DNA sequence encoding Peltiger a membr anacea c y anobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( Supplementary Table S2 ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or 1C (Addgene #29654) vectors using ligation-independent cloning (LIC) [ 39 ], resulting in constructs carrying an N-terminal hexahistidine-SUMO tag (His 6 -SUMO) followed by a tobacco etch virus (TEV) cleavage site or an N-terminal hexahistidine-maltose binding protein tag (His 6 -MBP) followed by a TEV cleavage site, respec- tively. .. The tnsAB gene was amplified by PCR with the primers TnsAB_f and TnsAB_r ( Supplementary Table S2 ) using the vector pHelper (PmcCAST)_PmcPSP1 (Addgene #168151) as a template.

    Article Title: Structure Characterization of Zinc Finger Motif 1 and 2 of GLI1 DNA Binding Region.
    Article Snippet: .. Molecular Cloning, Protein Expression, and Purification The yeast SUMO family protein SMT3 gene followed by the TEV cleavage site (SUMO-TEV) was amplified from a pET-His6-SUMO-TEV-LIC plasmid (Addgene #: 29659) via PCR using Phusion high-fidelity DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA), a forward primer (GGAATTCCATATGTCGGACTCAGAAGTCAATCAAGAAGC), and a reverse primer (CGCGGATCCGGATTGGAAGTACAGGTTTTCCTCGATC). ..

    Article Title: Bni5 regulates and coordinates septin architecture and myosin-II functions at the cell division site
    Article Snippet: .. The PCR products were subcloned into BamHI- and SspI-digested pET-His6-Sumo-TEV-LIC (#29659; Addgene) using In-Fusion HD cloning kit (Takara). ..

    Cloning:

    Article Title: Bni5 regulates and coordinates septin architecture and myosin-II functions at the cell division site.
    Article Snippet: .. The PCR products were subcloned into BamHI- and SspI-digested pET-His6Sumo-TEV-LIC (#29659; Addgene) using In-Fusion HD cloning kit (Takara). ..

    Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons.
    Article Snippet: The DNA sequence encoding Peltiger a membr anacea c y anobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( Supplementary Table S2 ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or 1C (Addgene #29654) vectors using ligation-independent cloning (LIC) [ 39 ], resulting in constructs carrying an N-terminal hexahistidine-SUMO tag (His 6 -SUMO) followed by a tobacco etch virus (TEV) cleavage site or an N-terminal hexahistidine-maltose binding protein tag (His 6 -MBP) followed by a TEV cleavage site, respec- tively. .. The tnsAB gene was amplified by PCR with the primers TnsAB_f and TnsAB_r ( Supplementary Table S2 ) using the vector pHelper (PmcCAST)_PmcPSP1 (Addgene #168151) as a template.

    Article Title: Bni5 regulates and coordinates septin architecture and myosin-II functions at the cell division site
    Article Snippet: .. The PCR products were subcloned into BamHI- and SspI-digested pET-His6-Sumo-TEV-LIC (#29659; Addgene) using In-Fusion HD cloning kit (Takara). ..

    Plasmid Preparation:

    Article Title: LOPAC library screening identifies suramin as a TRIM21 binder with a unique binding mode revealed by crystal structure
    Article Snippet: .. The human TRIM21 PRYSPRY domain (residues Val287–Leu465; UniProt ID P19474) with an N-terminal His 6 -tag followed by a SUMO tag in a pET His 6 SUMO LIC vector (Addgene) was transformed into Escherichia coli BL21(DE3) cells. ..

    Article Title: Structure Characterization of Zinc Finger Motif 1 and 2 of GLI1 DNA Binding Region.
    Article Snippet: .. Molecular Cloning, Protein Expression, and Purification The yeast SUMO family protein SMT3 gene followed by the TEV cleavage site (SUMO-TEV) was amplified from a pET-His6-SUMO-TEV-LIC plasmid (Addgene #: 29659) via PCR using Phusion high-fidelity DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA), a forward primer (GGAATTCCATATGTCGGACTCAGAAGTCAATCAAGAAGC), and a reverse primer (CGCGGATCCGGATTGGAAGTACAGGTTTTCCTCGATC). ..

    Transformation Assay:

    Article Title: LOPAC library screening identifies suramin as a TRIM21 binder with a unique binding mode revealed by crystal structure
    Article Snippet: .. The human TRIM21 PRYSPRY domain (residues Val287–Leu465; UniProt ID P19474) with an N-terminal His 6 -tag followed by a SUMO tag in a pET His 6 SUMO LIC vector (Addgene) was transformed into Escherichia coli BL21(DE3) cells. ..

    Ligation:

    Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons.
    Article Snippet: The DNA sequence encoding Peltiger a membr anacea c y anobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( Supplementary Table S2 ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or 1C (Addgene #29654) vectors using ligation-independent cloning (LIC) [ 39 ], resulting in constructs carrying an N-terminal hexahistidine-SUMO tag (His 6 -SUMO) followed by a tobacco etch virus (TEV) cleavage site or an N-terminal hexahistidine-maltose binding protein tag (His 6 -MBP) followed by a TEV cleavage site, respec- tively. .. The tnsAB gene was amplified by PCR with the primers TnsAB_f and TnsAB_r ( Supplementary Table S2 ) using the vector pHelper (PmcCAST)_PmcPSP1 (Addgene #168151) as a template.

    Construct:

    Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons.
    Article Snippet: The DNA sequence encoding Peltiger a membr anacea c y anobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( Supplementary Table S2 ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or 1C (Addgene #29654) vectors using ligation-independent cloning (LIC) [ 39 ], resulting in constructs carrying an N-terminal hexahistidine-SUMO tag (His 6 -SUMO) followed by a tobacco etch virus (TEV) cleavage site or an N-terminal hexahistidine-maltose binding protein tag (His 6 -MBP) followed by a TEV cleavage site, respec- tively. .. The tnsAB gene was amplified by PCR with the primers TnsAB_f and TnsAB_r ( Supplementary Table S2 ) using the vector pHelper (PmcCAST)_PmcPSP1 (Addgene #168151) as a template.

    Article Title: Molecular basis for dual-spacer-guided target cleavage by the TIGR-TasH system.
    Article Snippet: Article Molecular basis for dual-spacer-guided target cleavage by the TIGR-TasH system

    Virus:

    Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons.
    Article Snippet: The DNA sequence encoding Peltiger a membr anacea c y anobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( Supplementary Table S2 ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or 1C (Addgene #29654) vectors using ligation-independent cloning (LIC) [ 39 ], resulting in constructs carrying an N-terminal hexahistidine-SUMO tag (His 6 -SUMO) followed by a tobacco etch virus (TEV) cleavage site or an N-terminal hexahistidine-maltose binding protein tag (His 6 -MBP) followed by a TEV cleavage site, respec- tively. .. The tnsAB gene was amplified by PCR with the primers TnsAB_f and TnsAB_r ( Supplementary Table S2 ) using the vector pHelper (PmcCAST)_PmcPSP1 (Addgene #168151) as a template.

    Binding Assay:

    Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons.
    Article Snippet: The DNA sequence encoding Peltiger a membr anacea c y anobiont 210A (Pmc) TnsC was codon optimized for heterologous expression in E. coli , synthesized by GeneArt with flanking overhangs (Thermo Fisher Scientific) and amplified from the template using the primers TnsC_f and TnsC_r ( Supplementary Table S2 ). .. Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or 1C (Addgene #29654) vectors using ligation-independent cloning (LIC) [ 39 ], resulting in constructs carrying an N-terminal hexahistidine-SUMO tag (His 6 -SUMO) followed by a tobacco etch virus (TEV) cleavage site or an N-terminal hexahistidine-maltose binding protein tag (His 6 -MBP) followed by a TEV cleavage site, respec- tively. .. The tnsAB gene was amplified by PCR with the primers TnsAB_f and TnsAB_r ( Supplementary Table S2 ) using the vector pHelper (PmcCAST)_PmcPSP1 (Addgene #168151) as a template.

    Molecular Cloning:

    Article Title: Structure Characterization of Zinc Finger Motif 1 and 2 of GLI1 DNA Binding Region.
    Article Snippet: .. Molecular Cloning, Protein Expression, and Purification The yeast SUMO family protein SMT3 gene followed by the TEV cleavage site (SUMO-TEV) was amplified from a pET-His6-SUMO-TEV-LIC plasmid (Addgene #: 29659) via PCR using Phusion high-fidelity DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA), a forward primer (GGAATTCCATATGTCGGACTCAGAAGTCAATCAAGAAGC), and a reverse primer (CGCGGATCCGGATTGGAAGTACAGGTTTTCCTCGATC). ..

    Expressing:

    Article Title: Structure Characterization of Zinc Finger Motif 1 and 2 of GLI1 DNA Binding Region.
    Article Snippet: .. Molecular Cloning, Protein Expression, and Purification The yeast SUMO family protein SMT3 gene followed by the TEV cleavage site (SUMO-TEV) was amplified from a pET-His6-SUMO-TEV-LIC plasmid (Addgene #: 29659) via PCR using Phusion high-fidelity DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA), a forward primer (GGAATTCCATATGTCGGACTCAGAAGTCAATCAAGAAGC), and a reverse primer (CGCGGATCCGGATTGGAAGTACAGGTTTTCCTCGATC). ..

    Article Title: Molecular basis for dual-spacer-guided target cleavage by the TIGR-TasH system.
    Article Snippet: Article Molecular basis for dual-spacer-guided target cleavage by the TIGR-TasH system

    Purification:

    Article Title: Structure Characterization of Zinc Finger Motif 1 and 2 of GLI1 DNA Binding Region.
    Article Snippet: .. Molecular Cloning, Protein Expression, and Purification The yeast SUMO family protein SMT3 gene followed by the TEV cleavage site (SUMO-TEV) was amplified from a pET-His6-SUMO-TEV-LIC plasmid (Addgene #: 29659) via PCR using Phusion high-fidelity DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA), a forward primer (GGAATTCCATATGTCGGACTCAGAAGTCAATCAAGAAGC), and a reverse primer (CGCGGATCCGGATTGGAAGTACAGGTTTTCCTCGATC). ..

    Amplification:

    Article Title: Structure Characterization of Zinc Finger Motif 1 and 2 of GLI1 DNA Binding Region.
    Article Snippet: .. Molecular Cloning, Protein Expression, and Purification The yeast SUMO family protein SMT3 gene followed by the TEV cleavage site (SUMO-TEV) was amplified from a pET-His6-SUMO-TEV-LIC plasmid (Addgene #: 29659) via PCR using Phusion high-fidelity DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA), a forward primer (GGAATTCCATATGTCGGACTCAGAAGTCAATCAAGAAGC), and a reverse primer (CGCGGATCCGGATTGGAAGTACAGGTTTTCCTCGATC). ..

    Sequencing:

    Article Title: Molecular basis for dual-spacer-guided target cleavage by the TIGR-TasH system.
    Article Snippet: Article Molecular basis for dual-spacer-guided target cleavage by the TIGR-TasH system

    Synthesized:

    Article Title: Molecular basis for dual-spacer-guided target cleavage by the TIGR-TasH system.
    Article Snippet: Article Molecular basis for dual-spacer-guided target cleavage by the TIGR-TasH system

    Modification:

    Article Title: Molecular basis for dual-spacer-guided target cleavage by the TIGR-TasH system.
    Article Snippet: Article Molecular basis for dual-spacer-guided target cleavage by the TIGR-TasH system

    other:

    Article Title: Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons
    Article Snippet: Polymerase chain reaction (PCR) products were inserted into the 1S (Addgene #29659) or 1C (Addgene #29654) vectors using ligation-independent cloning (LIC) [ ], resulting in constructs carrying an N-terminal hexahistidine-SUMO tag (His 6 -SUMO) followed by a tobacco etch virus (TEV) cleavage site or an N-terminal hexahistidine-maltose binding protein tag (His 6 -MBP) followed by a TEV cleavage site, respectively.



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