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adenoviral vectors expressing scrambled shrna (ad-shctrl  (Shanghai GenePharma)

 
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    Structured Review

    Shanghai GenePharma adenoviral vectors expressing scrambled shrna (ad-shctrl
    Adenoviral Vectors Expressing Scrambled Shrna (Ad Shctrl, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ad-shctrl/adenoviral+vectors+expressing+a+control+scrambled+sequence++ad+ctrl+/pmc07413143-52-26-31
    Average 90 stars, based on 1 article reviews
    adenoviral vectors expressing scrambled shrna (ad-shctrl - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Identification of a Novel lincRNA-p21-miR-181b-PTEN Signaling Cascade in Liver Fibrosis
    Article Snippet: Transforming growth factor- β 1 (TGF- β 1) was purchased from R&D Systems (Shanghai, China). .. Adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl) and adenoviral vectors expressing lincRNA-p21 (Ad-lincRNA-p21) were purchased from GenePharma biotechnology (Shanghai, China). .. Transforming growth factor- β 1 (TGF- β 1) was purchased from R&D Systems (Shanghai, China).

    Article Title: Kaempferol Inhibits Hepatic Stellate Cell Activation by Regulating miR-26b-5p/Jag1 Axis and Notch Pathway.
    Article Snippet: .. MiRNA negative control (miR-NC), miR-26b-5p mimics/ inhibitor, adenoviral vectors expressing Jag1 (Ad-Jag1), adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl), adenoviral vectors expressing shRNA against Jag1 (Ad-shJag1) and adenoviral vectors expressing the scrambled shRNA (Ad-shCtrl) were obtained from GenePharma biotechnology (Shanghai, China). .. The molecular weight and molecular formula of kaempferol (≥97% purity, Sigma, St Louis, MO, United States) are 286.24 and C15H10O6, respectively (Figure 1A).

    Article Title: HOTAIR Epigenetically Modulates PTEN Expression via MicroRNA-29b: A Novel Mechanism in Regulation of Liver Fibrosis
    Article Snippet: .. Moreover, siDNMT3b, siHOTAIR, the scrambled siRNA (siCtrl), Ad-shHOTAIR, adenoviral vectors expressing the scrambled shRNA (Ad-shCtrl), adenoviral vectors expressing HOTAIR (Ad-HOTAIR), and adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl) were purchased from GenePharma Biotechnology. ..

    Article Title: The role of lncRNA-ZFAS1 in liver fibrosis: insights into the miR-1953/TAZ axis.
    Article Snippet: Long non-coding RNA-Zinc finger antisense 1 (lncRNA-ZFAS1) is involved in the progression of several cancer types, yet its function in liver fibrosis remains unclear.. The purpose of this study was to examine ZFAS1 expression in liver fibrosis and explore its possible molecular mechanism.. ZFAS1 expression was measured in a liver fibrosis model and in activated hepatic stellate cells (HSCs).

    Article Title: Loss of lncRNA-SNHG7 Promotes the Suppression of Hepatic Stellate Cell Activation via miR-378a-3p and DVL2
    Article Snippet: .. Ad-shSNHG7, adenoviral vectors expressing scrambled shRNA (Ad-shCtrl), adenoviral vectors expressing SNHG7 (Ad-SNHG7), and adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl) were obtained from GenePharma biotechnology. ..

    Article Title: Circular RNA cMTO1 Promotes PTEN Expression Through Sponging miR-181b-5p in Liver Fibrosis
    Article Snippet: .. Adenoviral vectors expressing cMTO1 (Ad-cMTO1), adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl), adenoviral vectors expressing shRNA against cMTO1 (Ad-shcMTO1) and adenoviral vectors expressing the scrambled shRNA (Ad-shCtrl) were purchased from GenePharma biotechnology. ..

    Control:

    Article Title: Identification of a Novel lincRNA-p21-miR-181b-PTEN Signaling Cascade in Liver Fibrosis
    Article Snippet: Transforming growth factor- β 1 (TGF- β 1) was purchased from R&D Systems (Shanghai, China). .. Adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl) and adenoviral vectors expressing lincRNA-p21 (Ad-lincRNA-p21) were purchased from GenePharma biotechnology (Shanghai, China). .. Transforming growth factor- β 1 (TGF- β 1) was purchased from R&D Systems (Shanghai, China).

    Article Title: Kaempferol Inhibits Hepatic Stellate Cell Activation by Regulating miR-26b-5p/Jag1 Axis and Notch Pathway.
    Article Snippet: .. MiRNA negative control (miR-NC), miR-26b-5p mimics/ inhibitor, adenoviral vectors expressing Jag1 (Ad-Jag1), adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl), adenoviral vectors expressing shRNA against Jag1 (Ad-shJag1) and adenoviral vectors expressing the scrambled shRNA (Ad-shCtrl) were obtained from GenePharma biotechnology (Shanghai, China). .. The molecular weight and molecular formula of kaempferol (≥97% purity, Sigma, St Louis, MO, United States) are 286.24 and C15H10O6, respectively (Figure 1A).

    Article Title: HOTAIR Epigenetically Modulates PTEN Expression via MicroRNA-29b: A Novel Mechanism in Regulation of Liver Fibrosis
    Article Snippet: .. Moreover, siDNMT3b, siHOTAIR, the scrambled siRNA (siCtrl), Ad-shHOTAIR, adenoviral vectors expressing the scrambled shRNA (Ad-shCtrl), adenoviral vectors expressing HOTAIR (Ad-HOTAIR), and adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl) were purchased from GenePharma Biotechnology. ..

    Article Title: The role of lncRNA-ZFAS1 in liver fibrosis: insights into the miR-1953/TAZ axis.
    Article Snippet: Long non-coding RNA-Zinc finger antisense 1 (lncRNA-ZFAS1) is involved in the progression of several cancer types, yet its function in liver fibrosis remains unclear.. The purpose of this study was to examine ZFAS1 expression in liver fibrosis and explore its possible molecular mechanism.. ZFAS1 expression was measured in a liver fibrosis model and in activated hepatic stellate cells (HSCs).

    Article Title: Loss of lncRNA-SNHG7 Promotes the Suppression of Hepatic Stellate Cell Activation via miR-378a-3p and DVL2
    Article Snippet: .. Ad-shSNHG7, adenoviral vectors expressing scrambled shRNA (Ad-shCtrl), adenoviral vectors expressing SNHG7 (Ad-SNHG7), and adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl) were obtained from GenePharma biotechnology. ..

    Article Title: Circular RNA cMTO1 Promotes PTEN Expression Through Sponging miR-181b-5p in Liver Fibrosis
    Article Snippet: .. Adenoviral vectors expressing cMTO1 (Ad-cMTO1), adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl), adenoviral vectors expressing shRNA against cMTO1 (Ad-shcMTO1) and adenoviral vectors expressing the scrambled shRNA (Ad-shCtrl) were purchased from GenePharma biotechnology. ..

    Sequencing:

    Article Title: Identification of a Novel lincRNA-p21-miR-181b-PTEN Signaling Cascade in Liver Fibrosis
    Article Snippet: Transforming growth factor- β 1 (TGF- β 1) was purchased from R&D Systems (Shanghai, China). .. Adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl) and adenoviral vectors expressing lincRNA-p21 (Ad-lincRNA-p21) were purchased from GenePharma biotechnology (Shanghai, China). .. Transforming growth factor- β 1 (TGF- β 1) was purchased from R&D Systems (Shanghai, China).

    Article Title: Kaempferol Inhibits Hepatic Stellate Cell Activation by Regulating miR-26b-5p/Jag1 Axis and Notch Pathway.
    Article Snippet: .. MiRNA negative control (miR-NC), miR-26b-5p mimics/ inhibitor, adenoviral vectors expressing Jag1 (Ad-Jag1), adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl), adenoviral vectors expressing shRNA against Jag1 (Ad-shJag1) and adenoviral vectors expressing the scrambled shRNA (Ad-shCtrl) were obtained from GenePharma biotechnology (Shanghai, China). .. The molecular weight and molecular formula of kaempferol (≥97% purity, Sigma, St Louis, MO, United States) are 286.24 and C15H10O6, respectively (Figure 1A).

    Article Title: HOTAIR Epigenetically Modulates PTEN Expression via MicroRNA-29b: A Novel Mechanism in Regulation of Liver Fibrosis
    Article Snippet: .. Moreover, siDNMT3b, siHOTAIR, the scrambled siRNA (siCtrl), Ad-shHOTAIR, adenoviral vectors expressing the scrambled shRNA (Ad-shCtrl), adenoviral vectors expressing HOTAIR (Ad-HOTAIR), and adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl) were purchased from GenePharma Biotechnology. ..

    Article Title: The role of lncRNA-ZFAS1 in liver fibrosis: insights into the miR-1953/TAZ axis.
    Article Snippet: Long non-coding RNA-Zinc finger antisense 1 (lncRNA-ZFAS1) is involved in the progression of several cancer types, yet its function in liver fibrosis remains unclear.. The purpose of this study was to examine ZFAS1 expression in liver fibrosis and explore its possible molecular mechanism.. ZFAS1 expression was measured in a liver fibrosis model and in activated hepatic stellate cells (HSCs).

    Article Title: Loss of lncRNA-SNHG7 Promotes the Suppression of Hepatic Stellate Cell Activation via miR-378a-3p and DVL2
    Article Snippet: .. Ad-shSNHG7, adenoviral vectors expressing scrambled shRNA (Ad-shCtrl), adenoviral vectors expressing SNHG7 (Ad-SNHG7), and adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl) were obtained from GenePharma biotechnology. ..

    Article Title: Circular RNA cMTO1 Promotes PTEN Expression Through Sponging miR-181b-5p in Liver Fibrosis
    Article Snippet: .. Adenoviral vectors expressing cMTO1 (Ad-cMTO1), adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl), adenoviral vectors expressing shRNA against cMTO1 (Ad-shcMTO1) and adenoviral vectors expressing the scrambled shRNA (Ad-shCtrl) were purchased from GenePharma biotechnology. ..

    Negative Control:

    Article Title: Kaempferol Inhibits Hepatic Stellate Cell Activation by Regulating miR-26b-5p/Jag1 Axis and Notch Pathway.
    Article Snippet: .. MiRNA negative control (miR-NC), miR-26b-5p mimics/ inhibitor, adenoviral vectors expressing Jag1 (Ad-Jag1), adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl), adenoviral vectors expressing shRNA against Jag1 (Ad-shJag1) and adenoviral vectors expressing the scrambled shRNA (Ad-shCtrl) were obtained from GenePharma biotechnology (Shanghai, China). .. The molecular weight and molecular formula of kaempferol (≥97% purity, Sigma, St Louis, MO, United States) are 286.24 and C15H10O6, respectively (Figure 1A).

    Article Title: The role of lncRNA-ZFAS1 in liver fibrosis: insights into the miR-1953/TAZ axis.
    Article Snippet: Long non-coding RNA-Zinc finger antisense 1 (lncRNA-ZFAS1) is involved in the progression of several cancer types, yet its function in liver fibrosis remains unclear.. The purpose of this study was to examine ZFAS1 expression in liver fibrosis and explore its possible molecular mechanism.. ZFAS1 expression was measured in a liver fibrosis model and in activated hepatic stellate cells (HSCs).

    shRNA:

    Article Title: Kaempferol Inhibits Hepatic Stellate Cell Activation by Regulating miR-26b-5p/Jag1 Axis and Notch Pathway.
    Article Snippet: .. MiRNA negative control (miR-NC), miR-26b-5p mimics/ inhibitor, adenoviral vectors expressing Jag1 (Ad-Jag1), adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl), adenoviral vectors expressing shRNA against Jag1 (Ad-shJag1) and adenoviral vectors expressing the scrambled shRNA (Ad-shCtrl) were obtained from GenePharma biotechnology (Shanghai, China). .. The molecular weight and molecular formula of kaempferol (≥97% purity, Sigma, St Louis, MO, United States) are 286.24 and C15H10O6, respectively (Figure 1A).

    Article Title: HOTAIR Epigenetically Modulates PTEN Expression via MicroRNA-29b: A Novel Mechanism in Regulation of Liver Fibrosis
    Article Snippet: .. Moreover, siDNMT3b, siHOTAIR, the scrambled siRNA (siCtrl), Ad-shHOTAIR, adenoviral vectors expressing the scrambled shRNA (Ad-shCtrl), adenoviral vectors expressing HOTAIR (Ad-HOTAIR), and adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl) were purchased from GenePharma Biotechnology. ..

    Article Title: Loss of lncRNA-SNHG7 Promotes the Suppression of Hepatic Stellate Cell Activation via miR-378a-3p and DVL2
    Article Snippet: .. Ad-shSNHG7, adenoviral vectors expressing scrambled shRNA (Ad-shCtrl), adenoviral vectors expressing SNHG7 (Ad-SNHG7), and adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl) were obtained from GenePharma biotechnology. ..

    Article Title: Circular RNA cMTO1 Promotes PTEN Expression Through Sponging miR-181b-5p in Liver Fibrosis
    Article Snippet: .. Adenoviral vectors expressing cMTO1 (Ad-cMTO1), adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl), adenoviral vectors expressing shRNA against cMTO1 (Ad-shcMTO1) and adenoviral vectors expressing the scrambled shRNA (Ad-shCtrl) were purchased from GenePharma biotechnology. ..

    Transfection:

    Article Title: The role of lncRNA-ZFAS1 in liver fibrosis: insights into the miR-1953/TAZ axis.
    Article Snippet: Long non-coding RNA-Zinc finger antisense 1 (lncRNA-ZFAS1) is involved in the progression of several cancer types, yet its function in liver fibrosis remains unclear.. The purpose of this study was to examine ZFAS1 expression in liver fibrosis and explore its possible molecular mechanism.. ZFAS1 expression was measured in a liver fibrosis model and in activated hepatic stellate cells (HSCs).



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    Image Search Results


    Knockdown of Steap4 in cardiomyocytes partially rescued the fatal heart failure in TG mice. (A) Cumulative survival rates of TG mice in shCtrl group (n = 20) and sh Steap4 (n = 20) group were recorded. (B) Representative images of the heart. (C) Cardiac Slc40a1 , Steap4 , and Fth proteins were measured using western blot analysis, normalized to β-actin (n = 4). (D) Total iron levels in the heart and serum were measured (n = 4). (E) Long-axis M-mode echocardiogram images and its analysis (n = 8). (F) Cardiac Nppa , Nppb , Myh7 mRNA were measured using qPCR, normalized to β-actin (n = 4). (G) Masson trichrome staining images and quantification of fibrotic areas (n = 4). The data is presented as the mean value plus or minus the standard error of the mean. Statistical significance is denoted by *p < 0.05 and **p < 0.01.

    Journal: International Journal of Biological Sciences

    Article Title: Elevated SLC40A1 impairs cardiac function and exacerbates mitochondrial dysfunction, oxidative stress, and apoptosis in ischemic myocardia

    doi: 10.7150/ijbs.89368

    Figure Lengend Snippet: Knockdown of Steap4 in cardiomyocytes partially rescued the fatal heart failure in TG mice. (A) Cumulative survival rates of TG mice in shCtrl group (n = 20) and sh Steap4 (n = 20) group were recorded. (B) Representative images of the heart. (C) Cardiac Slc40a1 , Steap4 , and Fth proteins were measured using western blot analysis, normalized to β-actin (n = 4). (D) Total iron levels in the heart and serum were measured (n = 4). (E) Long-axis M-mode echocardiogram images and its analysis (n = 8). (F) Cardiac Nppa , Nppb , Myh7 mRNA were measured using qPCR, normalized to β-actin (n = 4). (G) Masson trichrome staining images and quantification of fibrotic areas (n = 4). The data is presented as the mean value plus or minus the standard error of the mean. Statistical significance is denoted by *p < 0.05 and **p < 0.01.

    Article Snippet: Ad-sh Slc40a1 and Ad-shCtrl were provided by GeneChem Technology (Shanghai, China), in the form of adenoviral vectors carrying shRNA- Slc40a1 .Protein expression was observed 48 hours after viral infection.

    Techniques: Western Blot, Staining