rneasy mini kit (Qiagen)
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Structured Review
Qiagen
rneasy mini kit
Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 236379 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actin+polymerization+depolymerization+kit/pm21532622-34-13-16?v=Qiagen
Average 99 stars, based on 236379 article reviews
Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 236379 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actin+polymerization+depolymerization+kit/pm21532622-34-13-16?v=Qiagen
Average 99 stars, based on 236379 article reviews
rneasy mini kit - by Bioz Stars,
2026-08
99/100 stars
Images
1) Product Images from "E2F1 suppresses Wnt/β-catenin activity through transactivation of β-catenin interacting protein ICAT."
Article Title: E2F1 suppresses Wnt/β-catenin activity through transactivation of β-catenin interacting protein ICAT.
Journal: Oncogene
doi: 10.1038/onc.2011.129
Figure Legend Snippet: Figure 1 Ectopic E2F1 upregulates ICAT expression. (a) Gene expression heatmap showing the representative genes that are responsive to E2F1 activation in Saos-2 cells expressing pBabe (vector control) or ER-E2F1. Saos-2 cells infected with a retrovirus expressing ER-E2F1 or an empty vector (pBabe) were treated with OHT (300nM) for the indicated time points. Total RNA was extracted using TRizol (Invitrogen, Carlsbad, CA, USA) and purified with the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. Reverse transcription was performed using an RNA Amplification kit (Ambion, Austin, TX, USA). The microarray hybridization was performed using the Illumina Gene Expression Sentrix BeadChip HumanRef-8_V2 (Illumina, San Diego, CA, USA), and data analysis was performed and viewed using GeneSpring software from Agilent Technologies (Agilent, Santa Clara, CA, USA). Red, genes with higher expression levels; green, genes with lower expression levels. (b) ICAT, SIAH1 and CCNE1 mRNA expression levels in Saos-2 ER-E2F1 cells upon E2F1 activation. Saos-2 ER-E2F1 or pBabe control cells were treated with OHT (300 nM) for 24 h and the total RNA was isolated as in (a). Quantitative real-time PCR (qRT–PCR) was performed on a PRISM 7900 Sequence Detection System (Applied Biosystems, Carlsbad, CA, USA) using TaqMan probes of ICAT, SIAH1 and CCNE1 (Applied Biosystems). Samples were normalized to the levels of 18S ribosomal RNA. (c) Expression levels of CCND1 and c-MYC, two key Wnt/b-catenin target genes, were downregulated in Saos-2 ER-E2F1 cells upon E2F1 activation. Cell treatment and qRT–PCR were conducted as in (a). (d) ICAT expression levels were determined in pBabe, ER-E2F1 or in mutant ER-E2F1 (E132) systems in both Saos-2 (left) and HCT116 cells in the presence or absence of cycloheximide (10 mg/ml, 8 h). (e) Western blot analysis showing the induction of ICAT and CyclinE1 protein expression in ER-E2F1-expressing Saos-2 (left) or HCT116 (right) cells, but not in pBabe or E132 cells, upon OHT treatment using mouse anti-ICAT (Sigma-Aldrich, St Louis, MO, USA) or anti-CyclinE1 antibody (Santa Cruz, Santa Cruz, CA, USA). b-Actin was used as a loading control.
Techniques Used: Expressing, Gene Expression, Activation Assay, Plasmid Preparation, Control, Infection, Reverse Transcription, Microarray, Hybridization, Software, Isolation, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Sequencing, Mutagenesis, Western Blot
