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actin filament depolymerization n  (Cytoskeleton Inc)


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    Cytoskeleton Inc actin filament depolymerization n
    Actin Filament Depolymerization N, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/actin+filament+depolymerization+n/Actin+polymerization+buffer/pm38454084-199-117-142
    Average 95 stars, based on 54 article reviews
    actin filament depolymerization n - by Bioz Stars, 2026-11
    95/100 stars

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    Polymer:

    Article Title: Copper ion-induced MYH9 polymerization executes cuproptosis.
    Article Snippet: .. The obtained MYH9 species (monomer or polymer) were incubated with G-actin (pre-clarified by ultracentrifugation as required, Cytoskeleton, Inc.) on ice for 1 h. Actin polymerization was then initiated by adding actin polymerization buffer (5 mM Tris-HCl, pH 8.0, 0.2 mM CaCl2, 50 mM KCl, 2 mM MgCl2 and 1 mM ATP) and incubating at room temperature for 45 min. After polymerization, samples were centrifuged at 150000 × g for 2 h at room temperature. .. The obtained MYH9 species (monomer or polymer) were incubated with G-actin (pre-clarified by ultracentrifugation as required, Cytoskeleton, Inc.) on ice for 1 h. Actin polymerization was then initiated by adding actin polymerization buffer (5 mM Tris-HCl, pH 8.0, 0.2 mM CaCl2, 50 mM KCl, 2 mM MgCl2 and 1 mM ATP) and incubating at room temperature for 45 min. After polymerization, samples were centrifuged at 150000 × g for 2 h at room temperature.

    Incubation:

    Article Title: Copper ion-induced MYH9 polymerization executes cuproptosis.
    Article Snippet: .. The obtained MYH9 species (monomer or polymer) were incubated with G-actin (pre-clarified by ultracentrifugation as required, Cytoskeleton, Inc.) on ice for 1 h. Actin polymerization was then initiated by adding actin polymerization buffer (5 mM Tris-HCl, pH 8.0, 0.2 mM CaCl2, 50 mM KCl, 2 mM MgCl2 and 1 mM ATP) and incubating at room temperature for 45 min. After polymerization, samples were centrifuged at 150000 × g for 2 h at room temperature. .. The obtained MYH9 species (monomer or polymer) were incubated with G-actin (pre-clarified by ultracentrifugation as required, Cytoskeleton, Inc.) on ice for 1 h. Actin polymerization was then initiated by adding actin polymerization buffer (5 mM Tris-HCl, pH 8.0, 0.2 mM CaCl2, 50 mM KCl, 2 mM MgCl2 and 1 mM ATP) and incubating at room temperature for 45 min. After polymerization, samples were centrifuged at 150000 × g for 2 h at room temperature.

    Article Title: Septin crosstalk with microtubules and actin is regulated by a GSK3-dependent phosphoswitch
    Article Snippet: Filamentous actin (F-actin) was polymerized in vitro from unlabeled monomeric rabbit skeletal muscle actin (G-actin; Cytoskeleton Inc., AKL99). .. G-actin (28 μM) was incubated in F-actin polymerization buffer (Cytoskeleton Inc., BSA02-001) containing 10 mM Tris-HCl (pH 7.5), 2 mM MgCl2, 50 mM KCl, 1 mM ATP, 5 mM Guanidine Carbonate (pH 7.5), and 4 mM DTT for 20 min at room temperature to induce filament formation. .. Polymerized F-actin was subsequently stabilized and fluorescently labeled by supplementation with iFluor647-phalloidin (Abcam, ab176759) and incubated for an additional 30 min at room temperature.

    Labeling:

    Article Title: Correction to: Combining Nanoscale Curvature and Polymer Osmotic Pressure for Efficient Giant Vesicle Assembly Under Physiological Conditions.
    Article Snippet: A myTXTL Pro Cell-Free Expression Kit was purchased from Daicel Arbor Biosciences (Ann Arbor, MI). .. Actin ( > 99% pure, rabbit skeletal muscle), actin: HiLyte Fluor 488 Labeled ( > 99% pure, rabbit skeletal muscle), fascin-1 (wild-type, human-recombinant), actin polymerization buffer (10 × stock), and general actin buffer (1 × stock) were purchased from Cytoskeleton, Inc. (Denver, Co). ..



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    actin filament depolymerization n  (Cytoskeleton Inc)
    95
    Cytoskeleton Inc actin filament depolymerization n
    Actin Filament Depolymerization N, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/actin+filament+depolymerization+n/Actin+polymerization+buffer/pm38454084-199-117-142
    Average 95 stars, based on 1 article reviews
    actin filament depolymerization n - by Bioz Stars, 2026-11
    95/100 stars
    		  Buy from Supplier

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