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synthetic fluorogenic substrates ac-devd-afc  (Bachem)

 
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    Bachem synthetic fluorogenic substrates ac-devd-afc
    Synthetic Fluorogenic Substrates Ac Devd Afc, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/fluorogenic+substrate+ac+devd+afc/pmc01539015-57-12-16
    Average 90 stars, based on 1 article reviews
    synthetic fluorogenic substrates ac-devd-afc - by Bioz Stars, 2026-11
    90/100 stars

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    Article Title: Comparative study of the differential cell death protecting effect of various ROS scavengers.
    Article Snippet: Biological Chemistry ‘Just Accepted’ Papers are papers published online, in advance of appearing in the print journal.. They have been peer-reviewed, accepted and are online published in manuscript form, but have not been copy edited, typeset, or proofread.. Copy editing may lead to small differences between the Just Accepted version and the final version.

    Article Title: Synergistic complex from plants Solanaceae exhibits cytotoxicity for the human hepatocellular carcinoma cell line HepG2
    Article Snippet: We gathered 40 μg of protein from cells, untreated and treated with the plant extract in the presence or absence of inhibitor z-VAD-fmk, to determine caspase activity and by measuring the proteolytic cleavage of the fluorogenic substrate Ac-DEVD-AFC (Bachem) as also shown in standard protocols [ ].

    Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
    Article Snippet: 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

    Article Title: Characterization of caspase-2 inhibitors based on specific sites of caspase-2-mediated proteolysis.
    Article Snippet: AFC fluorogenic substrates Z‐VDVAD‐AFC and AcDEVD‐AFC and control peptides AcVDVAD‐CHO and AcDEVD‐CHO were purchased from Bachem (Torrance).

    Article Title: Characterization of caspase-2 inhibitors based on specific sites of caspase-2-mediated proteolysis
    Article Snippet: AFC fluorogenic substrates Z-VDVAD-AFC and AcDEVD-AFC and control peptides AcVDVAD-CHO and AcDEVD-CHO were purchased from Bachem (Torrance/CA, USA).

    Article Title: Siramesine triggers cell death through destabilisation of mitochondria, but not lysosomes
    Article Snippet: After determining the protein concentration with a Bradford reagent (Bio-Rad, Hercules, CA, USA), 75 μ g of protein was transferred to a 96-well plate and caspase buffer without detergents was added to a final volume of 90 μ l. After 10-min incubation at 37 °C, 10 μ l fluorogenic substrate Ac-DEVD-AFC (Bachem AG) was added (final concentration of 10 μ M), followed by continuous measurements of substrate hydrolysis for at least 30 min at excitation and emission wavelengths of 400 and 505 nm, respectively, using a Tecan Saphire microplate reader (Tecan, Männedorf, Switzerland).

    Article Title: Regulation of death receptor signaling by the autophagy protein TP 53 INP 2
    Article Snippet: DEVDase activity and FACS analysis 30 μg of protein of untreated and treated cells in the presence or absence of inhibitors was used to determine caspase activity by measuring the cleavage of fluorogenic substrate Ac‐DEVD‐AFC (Bachem) using Tecan.

    Control:

    Article Title: Characterization of caspase-2 inhibitors based on specific sites of caspase-2-mediated proteolysis
    Article Snippet: Human recombinant Casp1, 6, 7, and 9 were purchased from BioVision (Milpitas/CA, USA). .. AFC fluorogenic substrates and control peptides (AcYVAD-CHO, AcVDVAD-CHO, AcDEVD-CHO, AcVEID-CHO, and AcLEHD-CHO) were purchased from Bachem (Torrance/CA, USA). ..



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    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, H) Caspase-3 activity determined using Ac-DEVD-AFC as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.

    Journal: bioRxiv

    Article Title: Blocking Orai1 constitutive activity inhibits B-cell cancer migration and synergistically acts with drugs to reduce B-CLL cell survival

    doi: 10.1101/2023.12.12.571243

    Figure Lengend Snippet: Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, H) Caspase-3 activity determined using Ac-DEVD-AFC as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.

    Article Snippet: As reported and described previously [ ], activity of caspase-3 was performed by using Ac-DEVD-AFC substrate (Enzo Life Sciences, France).

    Techniques: Staining, Flow Cytometry, Activity Assay, Two Tailed Test