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ac devd cho  (Selleck Chemicals)


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    Selleck Chemicals ac devd cho
    Ac Devd Cho, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 494 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac+devd+cho/Necrostatin-1/pm41896219-261-2-22
    Average 96 stars, based on 494 article reviews
    ac devd cho - by Bioz Stars, 2026-11
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    Article Snippet: Alternatively, cells were stained for Annexin V/PI as indicated by Invitrogen (V13245).

    Transfection:

    Article Title: GSTM5 suppresses ovarian cancer progression in association with RASSF2A promoter hypomethylation and ferroptosis.
    Article Snippet: Objective: Glutathione S-transferase Mu 5 (GSTM5) is a detoxification-related enzyme whose function in ovarian cancer (OC) remains incompletely understood.. This study investigated the association of GSTM5 with OC progression and examined whether mitochondrial dysfunction, ferroptosis-related injury, and Ras association domain family member 2 isoform A (RASSF2A) promoter methylation contribute to its effects.. Methods: Gain‐ and loss‐of‐function experiments were performed in OC cells.

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    ( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D ) JC-1 staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, <t>Ac-DEVD-CHO,</t> or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.
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    ( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D ) JC-1 staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, <t>Ac-DEVD-CHO,</t> or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.
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    ( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D ) JC-1 staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, <t>Ac-DEVD-CHO,</t> or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.
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    ( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D ) JC-1 staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, <t>Ac-DEVD-CHO,</t> or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.
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    Effects of BHJ on the expression and activity of apototic-related proteins in HepG2 cells. A Western blot analysis of apoptotic-associated protein levels in HepG2 cells; B , C Effect of BHJ on caspase 9, caspase 3 enzyme activities in HepG2 cells; D Inhibition of BHJ-induced apoptosis by caspase 9 and caspase 3 inhibitors in HepG2 cells. The values are expressed as means ± SD of triplicates tests. * P < 0.05 comparison between the absence and presence of inhibitor in the treatment group
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    Effects of BHJ on the expression and activity of apototic-related proteins in HepG2 cells. A Western blot analysis of apoptotic-associated protein levels in HepG2 cells; B , C Effect of BHJ on caspase 9, caspase 3 enzyme activities in HepG2 cells; D Inhibition of BHJ-induced apoptosis by caspase 9 and caspase 3 inhibitors in HepG2 cells. The values are expressed as means ± SD of triplicates tests. * P < 0.05 comparison between the absence and presence of inhibitor in the treatment group
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    MedChemExpress caspase 3 inhibitor ac devd cho
    Effects of BHJ on the expression and activity of apototic-related proteins in HepG2 cells. A Western blot analysis of apoptotic-associated protein levels in HepG2 cells; B , C Effect of BHJ on caspase 9, caspase 3 enzyme activities in HepG2 cells; D Inhibition of BHJ-induced apoptosis by caspase 9 and caspase 3 inhibitors in HepG2 cells. The values are expressed as means ± SD of triplicates tests. * P < 0.05 comparison between the absence and presence of inhibitor in the treatment group
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    Effects of BHJ on the expression and activity of apototic-related proteins in HepG2 cells. A Western blot analysis of apoptotic-associated protein levels in HepG2 cells; B , C Effect of BHJ on caspase 9, caspase 3 enzyme activities in HepG2 cells; D Inhibition of BHJ-induced apoptosis by caspase 9 and caspase 3 inhibitors in HepG2 cells. The values are expressed as means ± SD of triplicates tests. * P < 0.05 comparison between the absence and presence of inhibitor in the treatment group
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    Effects of BHJ on the expression and activity of apototic-related proteins in HepG2 cells. A Western blot analysis of apoptotic-associated protein levels in HepG2 cells; B , C Effect of BHJ on caspase 9, caspase 3 enzyme activities in HepG2 cells; D Inhibition of BHJ-induced apoptosis by caspase 9 and caspase 3 inhibitors in HepG2 cells. The values are expressed as means ± SD of triplicates tests. * P < 0.05 comparison between the absence and presence of inhibitor in the treatment group
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    Image Search Results


    ( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D ) JC-1 staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, Ac-DEVD-CHO, or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.

    Journal: Science Advances

    Article Title: Chemo-metallo nanodrivers for spatiotemporally controlled in situ vaccination

    doi: 10.1126/sciadv.aee4577

    Figure Lengend Snippet: ( A ) Volcano plot of differentially expressed genes (DEGs) in MFC cells (Control versus DDP + Zn 2+ ). ( B ) KEGG pathway enrichment of DEGs. ( C ) Quantification of extracellular dsDNA. ( D ) JC-1 staining assessed by fluorescence microscopy and flow cytometry. ( E ) γ-H2AX immunofluorescence staining. Scale bar, 20 μm. ( F ) ASC speck colocalization with AIM2, Caspase-1, Caspase-8, and RIPK1. Scale bars, 5 μm (overview) and 1 μm (enlarged). ( G ) Western blot analysis of necroptosis markers (RIPK1, p-RIPK1, MLKL, and p-MLKL) and PANoptosis-related proteins (AIM2 and ZBP1). ( H ) Western blot analysis of apoptosis executors (Caspase-8, Caspase-3, and cleaved forms). ( I ) Western blot analysis of pyroptosis markers (Caspase-1, GSDMD, and cleaved forms). ( J ) Cell viability after DDP + Zn 2+ combined with VX-765, Ac-DEVD-CHO, or Nec-1. ( K ) PANoptosis-related proteins in si AIM2 cells. ( L and M ) cGAS activity (L) and expression (M) under Zn 2+ treatment. ( N ) Violin plot of cGAS expression. ( O ) Western blot analysis of STING pathway (STING, p-STING, TBK1, p-TBK1, IRF3, and p-IRF3). ( P ) CIBERSORT analysis of immune cell infiltration. ( Q ) BMDC maturation (CD80 + CD86 + ) after different treatments. ( R ) Schematic of the DDP + Zn 2+ –induced ICD. Data in (C), (J), and (L) are mean ± SD. Statistical analysis for (C), (J), (L), (N), and (P) used a two-tailed unpaired Student’s t test. (R) was created in BioRender. Fei, G. (2026) https://BioRender.com/8ri399r . * P < 0.05.

    Article Snippet: The pharmacological inhibitors VX-765, Ac-DEVD-CHO, and Nec-1 were purchased from MedChemExpress (USA).

    Techniques: Control, Staining, Fluorescence, Microscopy, Flow Cytometry, Immunofluorescence, Western Blot, Analysis, Activity Assay, Expressing, Immune Cell, Two Tailed Test

    Effects of BHJ on the expression and activity of apototic-related proteins in HepG2 cells. A Western blot analysis of apoptotic-associated protein levels in HepG2 cells; B , C Effect of BHJ on caspase 9, caspase 3 enzyme activities in HepG2 cells; D Inhibition of BHJ-induced apoptosis by caspase 9 and caspase 3 inhibitors in HepG2 cells. The values are expressed as means ± SD of triplicates tests. * P < 0.05 comparison between the absence and presence of inhibitor in the treatment group

    Journal: BMC Complementary Medicine and Therapies

    Article Title: Antioxidant activity and cytotoxic effects of Benincasa hispida juice on HepG2 cells

    doi: 10.1186/s12906-026-05475-3

    Figure Lengend Snippet: Effects of BHJ on the expression and activity of apototic-related proteins in HepG2 cells. A Western blot analysis of apoptotic-associated protein levels in HepG2 cells; B , C Effect of BHJ on caspase 9, caspase 3 enzyme activities in HepG2 cells; D Inhibition of BHJ-induced apoptosis by caspase 9 and caspase 3 inhibitors in HepG2 cells. The values are expressed as means ± SD of triplicates tests. * P < 0.05 comparison between the absence and presence of inhibitor in the treatment group

    Article Snippet: To test the activity of caspase 3 and caspase 9, 10 μM of Z-LEHD-FMK (BioVision, CA, USA) or 20 μM Ac-DEVD-CHO (Beyotime, Haimen, China) were added and incubated for 2 h prior to addition of BHJ for 48 h, then cell viability was detected by alamar blue assay.

    Techniques: Expressing, Activity Assay, Western Blot, Analysis, Inhibition, Comparison