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kpl abts peroxidase substrate solution mix  (SeraCare Life Sciences)

 
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    SeraCare Life Sciences kpl abts peroxidase substrate solution mix
    Kpl Abts Peroxidase Substrate Solution Mix, supplied by SeraCare Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/abts+solution/kpl+abts+peroxidase+substrate+solution+mix/pmc08572342-63-16-22
    Average 90 stars, based on 1 article reviews
    kpl abts peroxidase substrate solution mix - by Bioz Stars, 2026-09
    90/100 stars

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    Incubation:

    Article Title: Pathogenic and transcriptomic differences of emerging SARS-CoV-2 variants in the Syrian golden hamster model
    Article Snippet: After three washes with PBST, the bound antibodies were labeled using 50 μl of 1:2,500 peroxidase anti-hamster IgG (H+L) (SeraCare Life Sciences) diluted in 1% skim milk in PBST. .. After incubation for 1 hour at room temperature and three washes with PBST, 50 μl of KPL ABTS peroxidase substrate solution mix (SeraCare Life Sciences) was added to each well, and the mixture was incubated for 30 min at room temperature. .. The optical density (OD) at 405 nm was measured using a GloMax ® explorer (Promega).

    Article Title: Pathogenic and transcriptomic differences of emerging SARS-CoV-2 variants in the Syrian golden hamster model
    Article Snippet: After three washes with PBST, the bound antibodies were labeled using 50 μl of 1:2500 peroxidase anti-hamster IgG (H+L) (SeraCare Life Sciences, cat#5220-0371) diluted in 1% skim milk in PBST. .. After incubation for 1 h at room temperature and three washes with PBST, 50 μl of KPL ABTS peroxidase substrate solution mix (SeraCare Life Sciences) was added to each well, and the mixture was incubated for 30 min at room temperature. .. The optical density (OD) at 405 nm was measured using a GloMax® explorer (Promega).

    Article Title: Rapid protection from COVID-19 in nonhuman primates vaccinated intramuscularly but not intranasally with a single dose of a recombinant vaccine
    Article Snippet: After 3 washes with PBST, the bound antibodies were labeled using 50 μl of 1:10,000 HRP-labeled anti-mouse IgG (H+L) (SeraCare Life Sciences) diluted in 1% skim milk in PBST. .. For all ELISAs, after incubation for 1 h at room temperature and 3 washes with PBST, 50 μl of KPL ABTS peroxidase substrate solution mix (SeraCare Life Sciences) was added to each well, and the mixture was incubated for 30 min at room temperature. .. The optical density (OD) at 405 nm was measured using a GloMax ® explorer (Promega).

    Article Title: Optimization of Single-Dose VSV-Based COVID-19 Vaccination in Hamsters.
    Article Snippet: After three washes with PBST, the bound antibodies were labeled using 50 ml of 1:2,500 peroxidase anti-hamster IgG (H+L) (SeraCare Life Sciences) diluted in 1% skim milk in PBST. .. After incubation for 1 h at room temperature and three washes with PBST, 50 ml of KPL ABTS peroxidase substrate solution mix (SeraCare Life Sciences) was added to each well, and the mixture was incubated for 30 min at room temperature. .. The optical density (OD) at 405 nm was measured using a GloMax® explorer (Promega) plate reader.

    Article Title: Development of an Enzyme-Linked Immunosorbent Assay to Determine the Expression Dynamics of Ebola Virus Soluble Glycoprotein during Infection
    Article Snippet: After washing 3 times with PBST, the bound antibodies were labeled by using 50 μL of 1:1000 peroxidase AffiniPure Donkey Anti-Rabbit IgG (H + L) (Jackson ImmunoResearch) diluted in 1% skim milk in PBST. .. After incubation for 1 h at room temperature and 3 PBST washes, 50 μL of KPL ABTS Peroxidase substrate solution mix (SeraCare Life Sciences) was added to each well, and the mixture was incubated for 15 min at room temperature. ..



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    ROS scavenging capabilities of the PLCS hydrogel. (A) Visual color changes of the ABTS + • solution after incubation with PLC and PLCS hydrogels over time. (B) and (C) UV–vis absorption spectra of ABTS + • solution following incubation with PLC and PLCS hydrogels at different time points. (D) Quantitative comparison of ABTS + • scavenging activity between PLC and PLCS hydrogels over time (n = 3). (E) and (F) UV–vis absorption spectra of •OH scavenging activity of PLC, PLCS hydrogels at different time points. (G) Statistical comparison of •OH scavenging efficiencies of PLC and PLCS hydrogels (n = 3). (H) O 2 • - scavenging ability of PLC and PLCS hydrogels over time (n = 3). (I) H 2 O 2 scavenging ability of PLC and PLCS hydrogels over time (n = 3). (J) Flow cytometric analysis of intracellular ROS levels using DCFH-DA staining in different treatment groups. (K) Quantification of DCFH-DA fluorescence intensity (n = 3). (L–N), Fluorescent microscopy images of PC12 cells stained with DCFH-DA, DHE, and DAPI after various treatments, along with corresponding fluorescence intensity quantification (n = 3). Statistically significant at ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

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    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair

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    Figure Lengend Snippet: ROS scavenging capabilities of the PLCS hydrogel. (A) Visual color changes of the ABTS + • solution after incubation with PLC and PLCS hydrogels over time. (B) and (C) UV–vis absorption spectra of ABTS + • solution following incubation with PLC and PLCS hydrogels at different time points. (D) Quantitative comparison of ABTS + • scavenging activity between PLC and PLCS hydrogels over time (n = 3). (E) and (F) UV–vis absorption spectra of •OH scavenging activity of PLC, PLCS hydrogels at different time points. (G) Statistical comparison of •OH scavenging efficiencies of PLC and PLCS hydrogels (n = 3). (H) O 2 • - scavenging ability of PLC and PLCS hydrogels over time (n = 3). (I) H 2 O 2 scavenging ability of PLC and PLCS hydrogels over time (n = 3). (J) Flow cytometric analysis of intracellular ROS levels using DCFH-DA staining in different treatment groups. (K) Quantification of DCFH-DA fluorescence intensity (n = 3). (L–N), Fluorescent microscopy images of PC12 cells stained with DCFH-DA, DHE, and DAPI after various treatments, along with corresponding fluorescence intensity quantification (n = 3). Statistically significant at ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

    Article Snippet: The specific procedures were as follows: First, 7 mM ABTS solution was thoroughly mixed with 2.45 mM potassium persulfate (K 2 S 2 O 8 ) (Macklin, China) solution and reacted overnight at 4 °C in the dark to generate a stable ABTS• + radical stock solution.

    Techniques: Incubation, Comparison, Activity Assay, Staining, Fluorescence, Microscopy