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Titertek Instruments abts substrate solution
Abts Substrate Solution, supplied by Titertek Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Cytosolic Proteins Contribute to Surface Plasminogen Recruitment of Neisseria meningitidis
Article Snippet: Finally, 50 μl of ABTS substrate solution was added to each well, and the absorbance at 414 nm was measured using a Titertek Multiskan after 5 min, 10 min, and 15 min.

Article Title: Chronic varied stress modulates experimental autoimmune encephalomyelitis in Wistar rats.
Article Snippet: Stress disturbs homeostasis by altering the equilibrium of various hormones which have a significant impact on immune responses.. Few studies have examined the influence of stressors on autoimmune disease in animal models.. In our work, we studied the effects of long-term exposure (14 days) to chronic varied stress (CVS) in a model of experimental autoimmune encephalomyelitis (EAE) in Wistar rats.

Enzyme-linked Immunosorbent Assay:

Article Title: Novel monoclonal antibodies detect elevated levels of the chemokine CCL18/DC-CK1 in serum and body fluids in pathological conditions.
Article Snippet: After washing 3 with PBS-Tween, the plates were incubated with 5 g/ml mouse anti-CCL18/DC-CK1 mAb (AZNCK18, AZN-CK18B, or AZN-CK18C) for 1 h at room temperature (RT) followed by 3 washing with PBS-Tween and incubation with 50 l/well horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (250 ng/ml, Zymed Laboratories, Inc., San Francisco, CA). .. After washing, the ELISA was developed using 3,3 ,5,5 -tetramethylbenzidine (TMB) substrate, and absorption was measured using a Titertek ELISA plate reader at 450 nm. ..

Electron Microscopy:

Article Title: Cytosolic Proteins Contribute to Surface Plasminogen Recruitment of Neisseria meningitidis
Article Snippet: .. Finally, 50 l of ABTS substrate solution was added to each well, and the absorbance at 414 nm was measured using a Titertek Multiskan after 5 min, 10 min, and 15 min. Electron microscopy. ..

Article Title: Cytosolic Proteins Contribute to Surface Plasminogen Recruitment of Neisseria meningitidis
Article Snippet: .. Finally, 50 μl of ABTS substrate solution was added to each well, and the absorbance at 414 nm was measured using a Titertek Multiskan after 5 min, 10 min, and 15 min. Electron microscopy. ..

Incubation:

Article Title: Time-course analysis of CD25 and HLA-DR expression on lymphocytes in interferon-beta 1b-treated multiple sclerosis patients.
Article Snippet: http://msj.sagepub.com/ Multiple Sclerosis http://msj.sagepub.com/content/4/3/174 The online version of this article can be found at: DOI: 10.1177/135245859800400316 1998 4: 174Mult Scler A M Ferrarini, S Sivieri, M Buttarello, A Facchinetti, P Perini and P Gallo multiple sclerosis patients 1b-treatedβTime-course analysis of CD25 and HLA-DR expression on lymphocytes in interferon- Published by: http://www.sagepublications.com can be found at:Multiple SclerosisAdditional services and information for http://msj.sagepub.com/cgi/alertsEmail Alerts: http://msj.sagepub.com/subscriptionsSubscriptions: http://www.sagepub.com/journalsReprints.navReprints: http://www.sagepub.com/journalsPermissions.navPermissions: http://msj.sagepub.com/content/4/3/174.refs.htmlCitations: What is This?. - Jun 1, 1998Version of Record >> at University of British Columbia Library on February 20, 2013msj.sagepub.comDownloaded from Time-course analysis of CD25 and HLA-DR expression on lymphocytes in interferon-b1b-treated multiple sclerosis patients AM Ferrarini1, S Sivieri1, M Buttarello2, A Facchinetti3, P Perini1 and P Gallo1 1Department of Neurological and Psychiatrical Sciences, Second Neurological Clinic, University of Padua, Italy; 2Laboratory of Clinical Chemistry, ULS-16, Padua and 3Department of Oncological and Surgical Sciences, University of Padua, Italy To identify immunological markers that could be used to monitor relapsing-remitting multiple sclerosis (RRMS) course/activity during interferon beta 1b (IFNb1b) therapy, we longitudinally studied HLA-DR and CD25 expression by T lymphocytes in 15 IFNb1b-treated RRMS patients.. Peripheral blood T cell subsets were analysed before therapy (T0), and after 1 (T1), 2 (T2), 3 (T3), 6 (T4) and 12 (T5) months after therapy initiation.

Spectrophotometry:

Article Title: Studies on the structure of NADH:ubiquinone oxidoreductase complex: topography of the subunits of the iron-sulfur flavoprotein component.
Article Snippet: A catalytic component of the bovine mitochondrial NADH:ubiquinone oxidoreductase complex (Complex I) is a soluble NADH dehydrogenase iron-sulfur flavoprotein (FP).. FP is composed of three subunits of M, 51,000,24,000, and 9,000, and contains FMN and two iron-sulfur clusters.. Previous studies by others with the use of various chemical probes had suggested that, except for an access for NADH to the 5l-kDa subunit, the FP polypeptides are buried within Complex I and shielded from the medium.



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ROS scavenging capabilities of the PLCS hydrogel. (A) Visual color changes of the <t>ABTS</t> + • solution after incubation with PLC and PLCS hydrogels over time. (B) and (C) UV–vis absorption spectra of ABTS + • solution following incubation with PLC and PLCS hydrogels at different time points. (D) Quantitative comparison of ABTS + • scavenging activity between PLC and PLCS hydrogels over time (n = 3). (E) and (F) UV–vis absorption spectra of •OH scavenging activity of PLC, PLCS hydrogels at different time points. (G) Statistical comparison of •OH scavenging efficiencies of PLC and PLCS hydrogels (n = 3). (H) O 2 • - scavenging ability of PLC and PLCS hydrogels over time (n = 3). (I) H 2 O 2 scavenging ability of PLC and PLCS hydrogels over time (n = 3). (J) Flow cytometric analysis of intracellular ROS levels using DCFH-DA staining in different treatment groups. (K) Quantification of DCFH-DA fluorescence intensity (n = 3). (L–N), Fluorescent microscopy images of PC12 cells stained with DCFH-DA, DHE, and DAPI after various treatments, along with corresponding fluorescence intensity quantification (n = 3). Statistically significant at ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.
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ROS scavenging capabilities of the PLCS hydrogel. (A) Visual color changes of the ABTS + • solution after incubation with PLC and PLCS hydrogels over time. (B) and (C) UV–vis absorption spectra of ABTS + • solution following incubation with PLC and PLCS hydrogels at different time points. (D) Quantitative comparison of ABTS + • scavenging activity between PLC and PLCS hydrogels over time (n = 3). (E) and (F) UV–vis absorption spectra of •OH scavenging activity of PLC, PLCS hydrogels at different time points. (G) Statistical comparison of •OH scavenging efficiencies of PLC and PLCS hydrogels (n = 3). (H) O 2 • - scavenging ability of PLC and PLCS hydrogels over time (n = 3). (I) H 2 O 2 scavenging ability of PLC and PLCS hydrogels over time (n = 3). (J) Flow cytometric analysis of intracellular ROS levels using DCFH-DA staining in different treatment groups. (K) Quantification of DCFH-DA fluorescence intensity (n = 3). (L–N), Fluorescent microscopy images of PC12 cells stained with DCFH-DA, DHE, and DAPI after various treatments, along with corresponding fluorescence intensity quantification (n = 3). Statistically significant at ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

Journal: Bioactive Materials

Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair

doi: 10.1016/j.bioactmat.2025.12.009

Figure Lengend Snippet: ROS scavenging capabilities of the PLCS hydrogel. (A) Visual color changes of the ABTS + • solution after incubation with PLC and PLCS hydrogels over time. (B) and (C) UV–vis absorption spectra of ABTS + • solution following incubation with PLC and PLCS hydrogels at different time points. (D) Quantitative comparison of ABTS + • scavenging activity between PLC and PLCS hydrogels over time (n = 3). (E) and (F) UV–vis absorption spectra of •OH scavenging activity of PLC, PLCS hydrogels at different time points. (G) Statistical comparison of •OH scavenging efficiencies of PLC and PLCS hydrogels (n = 3). (H) O 2 • - scavenging ability of PLC and PLCS hydrogels over time (n = 3). (I) H 2 O 2 scavenging ability of PLC and PLCS hydrogels over time (n = 3). (J) Flow cytometric analysis of intracellular ROS levels using DCFH-DA staining in different treatment groups. (K) Quantification of DCFH-DA fluorescence intensity (n = 3). (L–N), Fluorescent microscopy images of PC12 cells stained with DCFH-DA, DHE, and DAPI after various treatments, along with corresponding fluorescence intensity quantification (n = 3). Statistically significant at ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

Article Snippet: The specific procedures were as follows: First, 7 mM ABTS solution was thoroughly mixed with 2.45 mM potassium persulfate (K 2 S 2 O 8 ) (Macklin, China) solution and reacted overnight at 4 °C in the dark to generate a stable ABTS• + radical stock solution.

Techniques: Incubation, Comparison, Activity Assay, Staining, Fluorescence, Microscopy