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abts stop solution  (SeraCare Life Sciences)

 
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    SeraCare Life Sciences abts stop solution
    Abts Stop Solution, supplied by SeraCare Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/abts+solution/abts+stop+solution/pmc07383396-446-26-29
    Average 90 stars, based on 1 article reviews
    abts stop solution - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Effects of rearing temperature on immune functions in sockeye salmon (Oncorhynchus nerka).
    Article Snippet: To determine if the defences of sockeye salmon (Oncorhynchus nerka) raised in captivity are a#ected by the rearing temperature or their life-cycle stage, various indices of the humoral and cellular immune functions were measured in fish reared at either 8 or 12 C for their entire life-cycle.. Measures of humoral immunity included the commonly used haematological parameters, as well as measurements of complement, and lysozyme activity.. Cellular assays quantified the ability of macrophages from the anterior kidney to phagocytise Staphylococcus aureus cells, or the activities of certain bactericidal systems of those cells.

    Article Title: Incomplete glycosylation during prion infection unmasks a prion protein epitope that facilitates prion detection and strain discrimination
    Article Snippet: Plates were processed as described above and were developed using 2,2’- azino-di(3-ethylbenzathiazoline-6-sulfonate) (ABTS) substrate (1-Component) (Seracare Life Sciences, Milford, MA) at room temperature, and reactions were stopped with ABTS Stop Solution (Seracare Life Sciences, Milford, MA) after 15 to 20 m. Absorbance was read at 405 nm using a BioTek Elx808 Ultra Microplate Reader (Bio-Tek instruments, Inc., Winooski, VT).

    Article Title: Sequence of Pathogenic Events in Cynomolgus Macaques Infected with Aerosolized Monkeypox Virus
    Article Snippet: The development of the ELISA was stopped by using ABTS stop solution (Kirkegaard and Perry Laboratories).

    Article Title: A DNA-based vaccine protects against Crimean-Congo hemorrhagic fever virus disease in a Cynomolgus macaque model
    Article Snippet: Bound antibodies were detected with goat horseradish peroxidase conjugated anti-monkey IgG (Seracare, catalog KPL 074–11-021) at 1:2000 and plates developed with ABTS solution (Seracare).

    Article Title: Comparative performance of a licensed anthrax vaccine versus electroporation based delivery of a PA encoding DNA vaccine in rhesus macaques.
    Article Snippet: DNA vaccination is a promising immunization strategy that could be applied in the development of vaccines for a variety of prophylactic and therapeutic indications.. Utilizing anthrax protective antigen as a ccepted 19 October 2009 vailable online 5 November 2009

    Article Title: Development and Characterization of a Multiplex Assay to Quantify Complement-Fixing Antibodies against Dengue Virus
    Article Snippet: The reaction was stopped with 1x ABTS stop solution (Seracare Milford, MA, USA) and microplates were read at 405 nm using SpectraMax Plus 384 microplate reader (Molecular Devices, San Jose, CA, USA).

    Article Title: Antibody responses by chinook salmon (Oncorhynchus tshawytscha) and rainbow trout (Oncorhynchus mykiss) to various protein antigens.
    Article Snippet: One of the hallmarks of the fish immune response to a foreign protein is the production of antibodies which bind specifically to epitopes of the target molecule.. Because that antibody response is the product of interactions between several components of the immune system, quantification of the antibody response to a defined protein provides an attractive approach for examining changes in the humoral arm of the fish disease defences.. Measures of the antibody response have been used to help describe the impact of environmental conditions [1], physiological changes [2], vaccination [3], and pathogenic micro-organisms [4] on fish health.

    Article Title: Incomplete glycosylation during prion infection unmasks a prion protein epitope that facilitates prion detection and strain discrimination
    Article Snippet: Plates were processed as described above and developed using 2,2'-azino-di(3-ethylbenzathiazoline-6-sulfonate) (ABTS) substrate (1-Component) (Seracare Life Sciences, Milford, MA) at room temperature, and reactions were stopped with ABTS Stop Solution (Seracare Life Sciences, Milford, MA) after 15 to 20 min. Absorbance was read at 405 nm using a Bio-Tek Elx808 Ultra Microplate Reader (Bio-Tek instruments, Inc.).



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    ROS scavenging capabilities of the PLCS hydrogel. (A) Visual color changes of the ABTS + • solution after incubation with PLC and PLCS hydrogels over time. (B) and (C) UV–vis absorption spectra of ABTS + • solution following incubation with PLC and PLCS hydrogels at different time points. (D) Quantitative comparison of ABTS + • scavenging activity between PLC and PLCS hydrogels over time (n = 3). (E) and (F) UV–vis absorption spectra of •OH scavenging activity of PLC, PLCS hydrogels at different time points. (G) Statistical comparison of •OH scavenging efficiencies of PLC and PLCS hydrogels (n = 3). (H) O 2 • - scavenging ability of PLC and PLCS hydrogels over time (n = 3). (I) H 2 O 2 scavenging ability of PLC and PLCS hydrogels over time (n = 3). (J) Flow cytometric analysis of intracellular ROS levels using DCFH-DA staining in different treatment groups. (K) Quantification of DCFH-DA fluorescence intensity (n = 3). (L–N), Fluorescent microscopy images of PC12 cells stained with DCFH-DA, DHE, and DAPI after various treatments, along with corresponding fluorescence intensity quantification (n = 3). Statistically significant at ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

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    Figure Lengend Snippet: ROS scavenging capabilities of the PLCS hydrogel. (A) Visual color changes of the ABTS + • solution after incubation with PLC and PLCS hydrogels over time. (B) and (C) UV–vis absorption spectra of ABTS + • solution following incubation with PLC and PLCS hydrogels at different time points. (D) Quantitative comparison of ABTS + • scavenging activity between PLC and PLCS hydrogels over time (n = 3). (E) and (F) UV–vis absorption spectra of •OH scavenging activity of PLC, PLCS hydrogels at different time points. (G) Statistical comparison of •OH scavenging efficiencies of PLC and PLCS hydrogels (n = 3). (H) O 2 • - scavenging ability of PLC and PLCS hydrogels over time (n = 3). (I) H 2 O 2 scavenging ability of PLC and PLCS hydrogels over time (n = 3). (J) Flow cytometric analysis of intracellular ROS levels using DCFH-DA staining in different treatment groups. (K) Quantification of DCFH-DA fluorescence intensity (n = 3). (L–N), Fluorescent microscopy images of PC12 cells stained with DCFH-DA, DHE, and DAPI after various treatments, along with corresponding fluorescence intensity quantification (n = 3). Statistically significant at ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

    Article Snippet: The specific procedures were as follows: First, 7 mM ABTS solution was thoroughly mixed with 2.45 mM potassium persulfate (K 2 S 2 O 8 ) (Macklin, China) solution and reacted overnight at 4 °C in the dark to generate a stable ABTS• + radical stock solution.

    Techniques: Incubation, Comparison, Activity Assay, Staining, Fluorescence, Microscopy