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abts solution  (Merck KGaA)

 
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    Merck KGaA abts solution
    Abts Solution, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/abts+++solution/abts+solution/pmc11122231-210-3-47
    Average 90 stars, based on 1 article reviews
    abts solution - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Assessment of the Quality, Bioactive Compounds, and Antimicrobial Activity of Egyptian, Ethiopian, and Syrian Black Cumin Oils
    Article Snippet: The ascorbic acid, 5α-cholestane, phytosterols, all solvents, sodium hydroxide, and anhydrous pyridine were purchased from Sigma-Aldrich (St. Louis, MO, USA); while gallic acid, ABTS solution, and DPPH (≥90%) were sourced from Merck KGaA (Darmstadt, Germany).

    Article Title: Comparison of the Antioxidant and Cytoprotective Properties of Extracts from Different Cultivars of Cornus mas L.
    Article Snippet: In our study, 2,2-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS solution), 2,2-diphenyl-1-picrylhydrazyl (DPPH), N,N,N′,N′-tetramethylethylenediamine (TEMED), acrylamide/bisacrylamide, ammonium persulfate (APS), ascorbic acid (vitamin C), bovine serum albumin (BSA), ethanol (96%), glycine, methanol, Ponceau S, resazurin sodium salt (RES), sodium dodecyl sulfate (SDS), Trolox, trypsin-EDTA solution, Tris-HCl, Tris-Base, and Tween-20 were purchased from Merck KGaA (Darmstadt, Germany).

    Article Title: Evaluation of the Biological Activity of Hydrogel with Cornus mas L. Extract and Its Potential Use in Dermatology and Cosmetology
    Article Snippet: The first step was to prepare a mixture of 7 mM ABTS solution (Merck KGaA, Darmstadt, Germany) and 2.4 mM potassium persulfate in a 1:1 ratio and leave it at room temperature (about 22 °C for 14 h).

    Article Title: Evaluation of the Biological Activity of Hydrogel with Cornus mas L. Extract and Its Potential Use in Dermatology and Cosmetology.
    Article Snippet: The first step was to prepare a mixture of 7 mM ABTS solution (Merck KGaA, Darmstadt, Germany) and 2.4 mM potassium persulfate in a 1:1 ratio and leave it at room temperature (about 22 ◦C for 14 h).

    Article Title: Assessment of the Antioxidant and Photoprotective Properties of Cornus mas L. Extracts on HDF, HaCaT and A375 Cells Exposed to UVA Radiation
    Article Snippet: Initially, a 7 mM ABTS solution (Merck KGaA, Darmstadt, Germany) and a 2.4 mM potassium persulfate solution (Merck KGaA, Darmstadt, Germany) were mixed in equal proportions and left for 14 h. After 14 h, the prepared solution was diluted with PBS (VWR International, Radnor, PA, USA) to obtain an absorbance of 1 ± 0.04 at λ = 734 nm.

    Article Title: Comparison of the Antioxidant and Cytoprotective Properties of Extracts from Different Cultivars of Cornus mas L.
    Article Snippet: Initially, a mixture of 7 mM ABTS solution (Merck KGaA, Darmstadt, Germany) and 2.4 mM potassium persulfate (Warchem, Zakręt, Poland) was prepared in a 1:1 ratio.

    Article Title: Assessment of the Antioxidant and Photoprotective Properties of Cornus mas L. Extracts on HDF, HaCaT and A375 Cells Exposed to UVA Radiation
    Article Snippet: In our study, 2,2-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS solution), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), acetonitrile, ascorbic acid (vitamin C), L-DOPA, kojic acid, MS–grade formic acid, neutral red solution (NR), phenylmethylsulfonyl fluoride (PMSF), resazurin sodium salt (RES), Triton X-100, Trolox, trypsin-EDTA solution and tyrosinase from mushroom were purchased from Merck KGaA (Darmstadt, Germany).

    ABTS Assay:

    Article Title: Protective Effect of Urtica dioica Extract against Oxidative Stress in Human Skin Fibroblasts
    Article Snippet: .. For the ABTS assay, a mixture of 7 mM ABTS solution (Merck KGaA, Darmstadt, Germany) with 2.4 mM potassium persulfate at a 1:1 ratio was prepared and left at room temperature for 14 h. Afterwards, the solution was adjusted to an absorbance of 1.0 ± 0.04 at a wavelength of 734 nm. ..



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    ROS scavenging capabilities of the PLCS hydrogel. (A) Visual color changes of the <t>ABTS</t> + • solution after incubation with PLC and PLCS hydrogels over time. (B) and (C) UV–vis absorption spectra of ABTS + • solution following incubation with PLC and PLCS hydrogels at different time points. (D) Quantitative comparison of ABTS + • scavenging activity between PLC and PLCS hydrogels over time (n = 3). (E) and (F) UV–vis absorption spectra of •OH scavenging activity of PLC, PLCS hydrogels at different time points. (G) Statistical comparison of •OH scavenging efficiencies of PLC and PLCS hydrogels (n = 3). (H) O 2 • - scavenging ability of PLC and PLCS hydrogels over time (n = 3). (I) H 2 O 2 scavenging ability of PLC and PLCS hydrogels over time (n = 3). (J) Flow cytometric analysis of intracellular ROS levels using DCFH-DA staining in different treatment groups. (K) Quantification of DCFH-DA fluorescence intensity (n = 3). (L–N), Fluorescent microscopy images of PC12 cells stained with DCFH-DA, DHE, and DAPI after various treatments, along with corresponding fluorescence intensity quantification (n = 3). Statistically significant at ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.
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    ROS scavenging capabilities of the PLCS hydrogel. (A) Visual color changes of the ABTS + • solution after incubation with PLC and PLCS hydrogels over time. (B) and (C) UV–vis absorption spectra of ABTS + • solution following incubation with PLC and PLCS hydrogels at different time points. (D) Quantitative comparison of ABTS + • scavenging activity between PLC and PLCS hydrogels over time (n = 3). (E) and (F) UV–vis absorption spectra of •OH scavenging activity of PLC, PLCS hydrogels at different time points. (G) Statistical comparison of •OH scavenging efficiencies of PLC and PLCS hydrogels (n = 3). (H) O 2 • - scavenging ability of PLC and PLCS hydrogels over time (n = 3). (I) H 2 O 2 scavenging ability of PLC and PLCS hydrogels over time (n = 3). (J) Flow cytometric analysis of intracellular ROS levels using DCFH-DA staining in different treatment groups. (K) Quantification of DCFH-DA fluorescence intensity (n = 3). (L–N), Fluorescent microscopy images of PC12 cells stained with DCFH-DA, DHE, and DAPI after various treatments, along with corresponding fluorescence intensity quantification (n = 3). Statistically significant at ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

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    Figure Lengend Snippet: ROS scavenging capabilities of the PLCS hydrogel. (A) Visual color changes of the ABTS + • solution after incubation with PLC and PLCS hydrogels over time. (B) and (C) UV–vis absorption spectra of ABTS + • solution following incubation with PLC and PLCS hydrogels at different time points. (D) Quantitative comparison of ABTS + • scavenging activity between PLC and PLCS hydrogels over time (n = 3). (E) and (F) UV–vis absorption spectra of •OH scavenging activity of PLC, PLCS hydrogels at different time points. (G) Statistical comparison of •OH scavenging efficiencies of PLC and PLCS hydrogels (n = 3). (H) O 2 • - scavenging ability of PLC and PLCS hydrogels over time (n = 3). (I) H 2 O 2 scavenging ability of PLC and PLCS hydrogels over time (n = 3). (J) Flow cytometric analysis of intracellular ROS levels using DCFH-DA staining in different treatment groups. (K) Quantification of DCFH-DA fluorescence intensity (n = 3). (L–N), Fluorescent microscopy images of PC12 cells stained with DCFH-DA, DHE, and DAPI after various treatments, along with corresponding fluorescence intensity quantification (n = 3). Statistically significant at ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.

    Article Snippet: The specific procedures were as follows: First, 7 mM ABTS solution was thoroughly mixed with 2.45 mM potassium persulfate (K 2 S 2 O 8 ) (Macklin, China) solution and reacted overnight at 4 °C in the dark to generate a stable ABTS• + radical stock solution.

    Techniques: Incubation, Comparison, Activity Assay, Staining, Fluorescence, Microscopy