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monoclonal mouse anti abcg2 antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology monoclonal mouse anti abcg2 antibody
    Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target <t>ABCG2.</t> Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.
    Monoclonal Mouse Anti Abcg2 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 522 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/abcg2/ABCG2+Antibody/pmc12915154-2-0-8
    Average 96 stars, based on 522 article reviews
    monoclonal mouse anti abcg2 antibody - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays"

    Article Title: Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2026.104361

    Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target ABCG2. Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.
    Figure Legend Snippet: Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target ABCG2. Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.

    Techniques Used: Transfection, Plasmid Preparation, Control, Variant Assay, Incubation, Immunofluorescence

    Related Articles

    Derivative Assay:

    Article Title: Annexin-A1 Regulates Apoptosis-Autophagy Switch in a 5-FU-Resistant Colorectal Cancer Stem-Like Model Through the PI3K/AKT/mTOR Axis.
    Article Snippet: Colorectal cancer (CRC) remains a leading cause of cancer-related deaths globally, primarily due to treatment failure associated with metastasis and recurrence driven by chemoresistant cancer stem-like cells (CSCs).. Annexin A1 (Anx-A1) is implicated in regulating apoptosis and autophagy, two crucial mechanisms contributing to chemoresistance.. However, the precise role of Anx-A1 in modulating these mechanisms in CRC CSCs, specifically via the PI3K/AKT/mTOR pathway, is not well understood.

    Expressing:

    Article Title: Annexin-A1 Regulates Apoptosis-Autophagy Switch in a 5-FU-Resistant Colorectal Cancer Stem-Like Model Through the PI3K/AKT/mTOR Axis.
    Article Snippet: Colorectal cancer (CRC) remains a leading cause of cancer-related deaths globally, primarily due to treatment failure associated with metastasis and recurrence driven by chemoresistant cancer stem-like cells (CSCs).. Annexin A1 (Anx-A1) is implicated in regulating apoptosis and autophagy, two crucial mechanisms contributing to chemoresistance.. However, the precise role of Anx-A1 in modulating these mechanisms in CRC CSCs, specifically via the PI3K/AKT/mTOR pathway, is not well understood.

    Bioprocessing:

    Article Title: Resin Glycosides from Ipomoea funis as Inhibitors of P-Glycoprotein in Multidrug-Resistant Breast Carcinoma Cells.
    Article Snippet: P-gp inhibitor agents, reserpine (10 μM, 7551−5G, Merck) and verapamil (10 μM, R1131−1G, Sigma-Aldrich) were used as positive controls.11 .. The reversal of MDR mediated by P-gp and the mechanism of cellular death was evaluated using the following monoclonal antibodies against: caspase-3 (sc-56053, Santa Cruz Biotechnology), ABCB1 (sc55510, Santa Cruz Biotechnology), ABCG2 (sc-58224, Santa Cruz Biotechnology), horseradish peroxidase (HRP)-conjugated β-actin loading control monoclonal antibody (sc-47778, Santa Cruz Biotechnology), and HRP-conjugated antimouse IgG secondary antibody (ab6728, Abcam). .. Cells were collected from 6-well plates (5 × 104 cells/well), which were treated with or without different concentrations of compound 2 for 48 h. Later, cells were treated with lysis buffer containing 292 mM saccharose, 100 mM Tris (pH 7.6), 5 mM magnesium chloride (MgCl2), 0.1% (w/v) sodium dodecyl sulfate (SDS), 1% (w/v) nonyl phenoxypolyethoxylethanol (NP40), 5 mM ethylenediamine tetraacetic acid (EDTA), and protease inhibitor (P9599, Sigma-Aldrich).

    Article Title: Resin Glycosides from Ipomoea funis as Inhibitors of P‑Glycoprotein in Multidrug-Resistant Breast Carcinoma Cells
    Article Snippet: P-gp inhibitor agents, reserpine (10 μM, 7551–5G, Merck) and verapamil (10 μM, R1131–1G, Sigma-Aldrich) were used as positive controls. .. The reversal of MDR mediated by P-gp and the mechanism of cellular death was evaluated using the following monoclonal antibodies against: caspase-3 (sc-56053, Santa Cruz Biotechnology), ABCB1 (sc-55510, Santa Cruz Biotechnology), ABCG2 (sc-58224, Santa Cruz Biotechnology), horseradish peroxidase (HRP)-conjugated β-actin loading control monoclonal antibody (sc-47778, Santa Cruz Biotechnology), and HRP-conjugated antimouse IgG secondary antibody (ab6728, Abcam). .. Cells were collected from 6-well plates (5 × 10 4 cells/well), which were treated with or without different concentrations of compound 2 for 48 h. Later, cells were treated with lysis buffer containing 292 mM saccharose, 100 mM Tris (pH 7.6), 5 mM magnesium chloride (MgCl 2 ), 0.1% (w/v) sodium dodecyl sulfate (SDS), 1% (w/v) nonyl phenoxypolyethoxylethanol (NP40), 5 mM ethylenediamine tetraacetic acid (EDTA), and protease inhibitor (P9599, Sigma-Aldrich).

    Control:

    Article Title: Resin Glycosides from Ipomoea funis as Inhibitors of P-Glycoprotein in Multidrug-Resistant Breast Carcinoma Cells.
    Article Snippet: P-gp inhibitor agents, reserpine (10 μM, 7551−5G, Merck) and verapamil (10 μM, R1131−1G, Sigma-Aldrich) were used as positive controls.11 .. The reversal of MDR mediated by P-gp and the mechanism of cellular death was evaluated using the following monoclonal antibodies against: caspase-3 (sc-56053, Santa Cruz Biotechnology), ABCB1 (sc55510, Santa Cruz Biotechnology), ABCG2 (sc-58224, Santa Cruz Biotechnology), horseradish peroxidase (HRP)-conjugated β-actin loading control monoclonal antibody (sc-47778, Santa Cruz Biotechnology), and HRP-conjugated antimouse IgG secondary antibody (ab6728, Abcam). .. Cells were collected from 6-well plates (5 × 104 cells/well), which were treated with or without different concentrations of compound 2 for 48 h. Later, cells were treated with lysis buffer containing 292 mM saccharose, 100 mM Tris (pH 7.6), 5 mM magnesium chloride (MgCl2), 0.1% (w/v) sodium dodecyl sulfate (SDS), 1% (w/v) nonyl phenoxypolyethoxylethanol (NP40), 5 mM ethylenediamine tetraacetic acid (EDTA), and protease inhibitor (P9599, Sigma-Aldrich).

    Article Title: Resin Glycosides from Ipomoea funis as Inhibitors of P‑Glycoprotein in Multidrug-Resistant Breast Carcinoma Cells
    Article Snippet: P-gp inhibitor agents, reserpine (10 μM, 7551–5G, Merck) and verapamil (10 μM, R1131–1G, Sigma-Aldrich) were used as positive controls. .. The reversal of MDR mediated by P-gp and the mechanism of cellular death was evaluated using the following monoclonal antibodies against: caspase-3 (sc-56053, Santa Cruz Biotechnology), ABCB1 (sc-55510, Santa Cruz Biotechnology), ABCG2 (sc-58224, Santa Cruz Biotechnology), horseradish peroxidase (HRP)-conjugated β-actin loading control monoclonal antibody (sc-47778, Santa Cruz Biotechnology), and HRP-conjugated antimouse IgG secondary antibody (ab6728, Abcam). .. Cells were collected from 6-well plates (5 × 10 4 cells/well), which were treated with or without different concentrations of compound 2 for 48 h. Later, cells were treated with lysis buffer containing 292 mM saccharose, 100 mM Tris (pH 7.6), 5 mM magnesium chloride (MgCl 2 ), 0.1% (w/v) sodium dodecyl sulfate (SDS), 1% (w/v) nonyl phenoxypolyethoxylethanol (NP40), 5 mM ethylenediamine tetraacetic acid (EDTA), and protease inhibitor (P9599, Sigma-Aldrich).

    other:

    Article Title: Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays
    Article Snippet: Monoclonal mouse anti-ABCG2 Antibody (BXP-21) (PLA 1:100) , Santa Cruz Biotechnology , Cat#sc-58222; RRID: AB_630828.

    Immunohistochemistry:

    Article Title: Globo H ceramide confers chemoresistance and poor prognosis to advanced gallbladder cancer via A2AR/cAMP/PKA pathway
    Article Snippet: .. Antibodies against ABCG2 (sc-69988, Santa Cruz Biotechnology, USA) and Ki67 (MIB-1, Dako, USA) were used for IHC. .. IHC staining for Globo H, ABCG2, and Ki67 was performed on FFPE human, PDX, and rat tumor tissues using the BOND RXm stainer and Leica BONDTM Polymer Refine Detection Kit (DS9800, Leica Biosystems, Vista, CA) according to the manufacturer's instructions.

    Proximity Ligation Assay:

    Article Title: Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays.
    Article Snippet: .. Antibodies Monoclonal mouse anti-ABCG2 Antibody (BXP-21) (PLA 1:100) Santa Cruz Biotechnology Catsc-58222; RRID: AB_630828 Monoclonal mouse anti-StrepII-tag Antibody (517) (PLA 1:400) Novus Biologicals Cat#NBP2-43735 Polyclonal rabbit anti-StrepII-tag Antibody (WB 1:2,000; PLA 1:450) Abcam Cat#ab76949; RRID: AB_1524455 Chemicals, peptides, and recombinant proteins Bovine serum albumin (BSA) KPL Cat#5140-0006 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat#41965039 (Continued on next page) STAR Protocols 7, 104361, March 20, 2026 5 ..

    Western Blot:

    Article Title: Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays.
    Article Snippet: .. Antibodies Monoclonal mouse anti-ABCG2 Antibody (BXP-21) (PLA 1:100) Santa Cruz Biotechnology Catsc-58222; RRID: AB_630828 Monoclonal mouse anti-StrepII-tag Antibody (517) (PLA 1:400) Novus Biologicals Cat#NBP2-43735 Polyclonal rabbit anti-StrepII-tag Antibody (WB 1:2,000; PLA 1:450) Abcam Cat#ab76949; RRID: AB_1524455 Chemicals, peptides, and recombinant proteins Bovine serum albumin (BSA) KPL Cat#5140-0006 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat#41965039 (Continued on next page) STAR Protocols 7, 104361, March 20, 2026 5 ..

    Recombinant:

    Article Title: Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays.
    Article Snippet: .. Antibodies Monoclonal mouse anti-ABCG2 Antibody (BXP-21) (PLA 1:100) Santa Cruz Biotechnology Catsc-58222; RRID: AB_630828 Monoclonal mouse anti-StrepII-tag Antibody (517) (PLA 1:400) Novus Biologicals Cat#NBP2-43735 Polyclonal rabbit anti-StrepII-tag Antibody (WB 1:2,000; PLA 1:450) Abcam Cat#ab76949; RRID: AB_1524455 Chemicals, peptides, and recombinant proteins Bovine serum albumin (BSA) KPL Cat#5140-0006 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat#41965039 (Continued on next page) STAR Protocols 7, 104361, March 20, 2026 5 ..

    Modification:

    Article Title: Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays.
    Article Snippet: .. Antibodies Monoclonal mouse anti-ABCG2 Antibody (BXP-21) (PLA 1:100) Santa Cruz Biotechnology Catsc-58222; RRID: AB_630828 Monoclonal mouse anti-StrepII-tag Antibody (517) (PLA 1:400) Novus Biologicals Cat#NBP2-43735 Polyclonal rabbit anti-StrepII-tag Antibody (WB 1:2,000; PLA 1:450) Abcam Cat#ab76949; RRID: AB_1524455 Chemicals, peptides, and recombinant proteins Bovine serum albumin (BSA) KPL Cat#5140-0006 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat#41965039 (Continued on next page) STAR Protocols 7, 104361, March 20, 2026 5 ..



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    Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target <t>ABCG2.</t> Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.
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    Image Search Results


    Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target ABCG2. Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.

    Journal: STAR Protocols

    Article Title: Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays

    doi: 10.1016/j.xpro.2026.104361

    Figure Lengend Snippet: Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target ABCG2. Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.

    Article Snippet: Monoclonal mouse anti-ABCG2 Antibody (BXP-21) (PLA 1:100) , Santa Cruz Biotechnology , Cat#sc-58222; RRID: AB_630828.

    Techniques: Transfection, Plasmid Preparation, Control, Variant Assay, Incubation, Immunofluorescence