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gene exp abcg2 hs01053790 m1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher gene exp abcg2 hs01053790 m1
    Gene Exp Abcg2 Hs01053790 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/abcg2/Gene+Exp%2E+ABCG2%2C+Hs01053790_m1/pm41998207-59-1-23
    Average 99 stars, based on 1 article reviews
    gene exp abcg2 hs01053790 m1 - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    other:

    Article Title: Exploiting porphyrin metabolism to inhibit angiogenesis
    Article Snippet: ABCG2 , Hs01053790_m1.

    Article Title: Identification of novel drug-specific PARP inhibitor resistance mechanisms in ovarian cancer-implications for clinical practice.
    Article Snippet: Total RNA was extracted from 1 × 106 cells using RNeasy Mini Kits (Qiagen, Manchester, UK), following the Manufacturer’s instructions, including an on-column DNase digestion (RNase free DNase Kit, Qiagen, Manchester, UK).

    Sequencing:

    Article Title: Influence of fluid shear stress on human limbal epithelial cells
    Article Snippet: .. The following primers were used: NP63α (custom made primer sequence adapted from Robertson et al.) [ ] ABCG2 (Hs01053790_m1), CK15 (Hs00267035_m1), Nestin (Hs00707120_s1), CK14 (Hs00265033_m1), CK3 (Hs00365080_m1), CK12 (Hs00165015_m1), TRPV4 (Hs01099348_m1) and GAPDH (Hs02758991_g1) housekeeping gene control. ..

    Control:

    Article Title: Influence of fluid shear stress on human limbal epithelial cells
    Article Snippet: .. The following primers were used: NP63α (custom made primer sequence adapted from Robertson et al.) [ ] ABCG2 (Hs01053790_m1), CK15 (Hs00267035_m1), Nestin (Hs00707120_s1), CK14 (Hs00265033_m1), CK3 (Hs00365080_m1), CK12 (Hs00165015_m1), TRPV4 (Hs01099348_m1) and GAPDH (Hs02758991_g1) housekeeping gene control. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Exploiting porphyrin metabolism to inhibit angiogenesis.
    Article Snippet: Total RNA was extracted using PureLink RNA Mini Kit (Thermo Fisher Scientific, Waltham, MA USA) and 0.5–1 ug of total RNA was transcribed into complementary DNA (cDNA) by High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA USA). .. Quantitative Table 1 Mouse Taqman assays Gene name Assay ID Alas1 Mm01235914_m1 Fech Mm00500394_m1 Hmox-1 Mm00516005_m1 Hpx Mm00457510_m1 Cdh5 Mm00486938_m1 Table 2 Human Taqman assays Gene name Assay ID ABCG2 Hs01053790_m1 ALAS1 Hs00167441_m1 FECH Hs00164616_m1 HPX Hs00167197_m1 FLVCR2 Hs00215055_m1 Table 3 Mouse qPCR primers (SYBR green assay) Gene name Forward primer Reverse primer Alad C T G C G C T G T G T C C T G A T C T C T G C A G A G C C C T G T T C A T C Hmbs A G A A A A G T G C C G T G G G A A C T G T T G A G G T T T C C C C G A A T Uros C A A G G G A G C C T G A A G A G C T A G G A G G G A C T G A A A A A C G T G A Cpox C T T T G A A G T A G A G G A A G C T G A C G T T C A A G T A T G T C G G T G T G A G G T Slc15a1 G A C C A C A A T G G C A G T C C T G A C A A T C G C C A C C A A A C G C A G A C A C A Slc15a2 G T G A A G G C A C T G A C C A G G A T A C T G T T A G C T T G C A G A G T C C A C C G Table 4 Human qPCR primers (SYBR green assay) Gene name Forward primer Reverse primer ..

    SYBR Green Assay:

    Article Title: Exploiting porphyrin metabolism to inhibit angiogenesis.
    Article Snippet: Total RNA was extracted using PureLink RNA Mini Kit (Thermo Fisher Scientific, Waltham, MA USA) and 0.5–1 ug of total RNA was transcribed into complementary DNA (cDNA) by High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA USA). .. Quantitative Table 1 Mouse Taqman assays Gene name Assay ID Alas1 Mm01235914_m1 Fech Mm00500394_m1 Hmox-1 Mm00516005_m1 Hpx Mm00457510_m1 Cdh5 Mm00486938_m1 Table 2 Human Taqman assays Gene name Assay ID ABCG2 Hs01053790_m1 ALAS1 Hs00167441_m1 FECH Hs00164616_m1 HPX Hs00167197_m1 FLVCR2 Hs00215055_m1 Table 3 Mouse qPCR primers (SYBR green assay) Gene name Forward primer Reverse primer Alad C T G C G C T G T G T C C T G A T C T C T G C A G A G C C C T G T T C A T C Hmbs A G A A A A G T G C C G T G G G A A C T G T T G A G G T T T C C C C G A A T Uros C A A G G G A G C C T G A A G A G C T A G G A G G G A C T G A A A A A C G T G A Cpox C T T T G A A G T A G A G G A A G C T G A C G T T C A A G T A T G T C G G T G T G A G G T Slc15a1 G A C C A C A A T G G C A G T C C T G A C A A T C G C C A C C A A A C G C A G A C A C A Slc15a2 G T G A A G G C A C T G A C C A G G A T A C T G T T A G C T T G C A G A G T C C A C C G Table 4 Human qPCR primers (SYBR green assay) Gene name Forward primer Reverse primer ..

    Gene Expression:

    Article Title: Bisphenol A triggers adipocyte dysfunction, thereby fostering triple-negative breast cancer aggressiveness.
    Article Snippet: Bisphenol A (BPA) is an organic compound widely used in the production of polycarbonate plastics and epoxy resins.. As a pervasive environmental pollutant, BPA accumulates in adipose tissue (AT) due to its lipophilic properties.. AT, an endocrine organ central to homeostasis, constitutes the main component of breast stroma and plays a pivotal role in the microenvironment of breast cancer, including triple-negative breast cancer (TNBC).

    Article Title: Anti-HER2-targeted therapies: effects on human in vitro blood-brain barrier models
    Article Snippet: The qPCR reaction was prepared using the TaqMan® Fast Advanced Master Mix (Thermo Fisher Scientific, 4444965). .. Specific gene expression was quantified using TaqMan® Gene Expression Assays (Thermo Fisher Scientific, 4331182) designed and validated for the following target genes: ABCB1 (Hs00184500_m1), ABCG2 (Hs01053790_m1), CCL2 (Hs00234140_m1), CLDN5 (Hs00533949_s1), EGFR (Hs01076090), ERBB2 (Hs01001580), OCLN (Hs00170162_m1), SLC2A1 (Hs00892681_m1). .. CANX (calnexin, Hs01558409_m1) was used as an endogenous control.



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    Effects of L-carnitine on intestinal morphology, barrier-related proteins, and urate transporter expression in hyperuricemic mice. (A) H&E staining of intestinal tissue sections. (B) Immunofluorescence staining of Occludin and ZO-1 in the small intestine ( n = 3 per group). (C) Representative protein expression of intestinal urate transport-related proteins. (D) Quantitative analysis of intestinal urate transport-related protein expression ( n = 3 per group). (E) Immunofluorescence staining of <t>ABCG2</t> in the small intestine ( n = 3 per group). Data are presented as mean ± SEM. ns, not significant; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CG; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HUG.
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    Effects of L-carnitine on intestinal morphology, barrier-related proteins, and urate transporter expression in hyperuricemic mice. (A) H&E staining of intestinal tissue sections. (B) Immunofluorescence staining of Occludin and ZO-1 in the small intestine ( n = 3 per group). (C) Representative protein expression of intestinal urate transport-related proteins. (D) Quantitative analysis of intestinal urate transport-related protein expression ( n = 3 per group). (E) Immunofluorescence staining of <t>ABCG2</t> in the small intestine ( n = 3 per group). Data are presented as mean ± SEM. ns, not significant; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CG; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HUG.
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    Effects of L-carnitine on intestinal morphology, barrier-related proteins, and urate transporter expression in hyperuricemic mice. (A) H&E staining of intestinal tissue sections. (B) Immunofluorescence staining of Occludin and ZO-1 in the small intestine ( n = 3 per group). (C) Representative protein expression of intestinal urate transport-related proteins. (D) Quantitative analysis of intestinal urate transport-related protein expression ( n = 3 per group). (E) Immunofluorescence staining of <t>ABCG2</t> in the small intestine ( n = 3 per group). Data are presented as mean ± SEM. ns, not significant; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CG; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HUG.
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    Effects of L-carnitine on intestinal morphology, barrier-related proteins, and urate transporter expression in hyperuricemic mice. (A) H&E staining of intestinal tissue sections. (B) Immunofluorescence staining of Occludin and ZO-1 in the small intestine ( n = 3 per group). (C) Representative protein expression of intestinal urate transport-related proteins. (D) Quantitative analysis of intestinal urate transport-related protein expression ( n = 3 per group). (E) Immunofluorescence staining of <t>ABCG2</t> in the small intestine ( n = 3 per group). Data are presented as mean ± SEM. ns, not significant; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CG; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HUG.
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    Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target <t>ABCG2.</t> Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.
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    Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target <t>ABCG2.</t> Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.
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    Image Search Results


    Effects of L-carnitine on intestinal morphology, barrier-related proteins, and urate transporter expression in hyperuricemic mice. (A) H&E staining of intestinal tissue sections. (B) Immunofluorescence staining of Occludin and ZO-1 in the small intestine ( n = 3 per group). (C) Representative protein expression of intestinal urate transport-related proteins. (D) Quantitative analysis of intestinal urate transport-related protein expression ( n = 3 per group). (E) Immunofluorescence staining of ABCG2 in the small intestine ( n = 3 per group). Data are presented as mean ± SEM. ns, not significant; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CG; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HUG.

    Journal: Frontiers in Nutrition

    Article Title: Oral L-carnitine supplementation lowers serum uric acid and is associated with renal and intestinal urate-handling responses in hyperuricemic mice

    doi: 10.3389/fnut.2026.1866695

    Figure Lengend Snippet: Effects of L-carnitine on intestinal morphology, barrier-related proteins, and urate transporter expression in hyperuricemic mice. (A) H&E staining of intestinal tissue sections. (B) Immunofluorescence staining of Occludin and ZO-1 in the small intestine ( n = 3 per group). (C) Representative protein expression of intestinal urate transport-related proteins. (D) Quantitative analysis of intestinal urate transport-related protein expression ( n = 3 per group). (E) Immunofluorescence staining of ABCG2 in the small intestine ( n = 3 per group). Data are presented as mean ± SEM. ns, not significant; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CG; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HUG.

    Article Snippet: After blocking with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies: XOD (1:2000, Cat. No. 55156-1-AP, Proteintech), ADA (1:2000, Cat. No. 67870-1-Ig, Proteintech), OAT1 (1:2000, Cat. No. 26574-1-AP, Proteintech), OAT3 (1:2000, Cat. No. bs-0609R, Bioss), GLUT9 (1:1000, Cat. No. 26486-1-AP, Proteintech), ABCG2 (1:1000, Cat. No. 27286-1-AP, Proteintech), URAT1 (1:3000, Cat. No. 14937-1-AP, Proteintech), IκBα (1:1000, Cat. No. GB111509 , Servicebio), NF-κB p65 (1:2000, Cat. No. 66535-1-Ig, Proteintech), phospho-NF-κB p65 (1:2000, Cat. No. ZHS1565, ZhongHe) and β-actin (1:8000, Cat. No. 66009-1-Ig, Proteintech).

    Techniques: Morphology, Expressing, Staining, Tissue, Immunofluorescence, Analysis

    Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target ABCG2. Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.

    Journal: STAR Protocols

    Article Title: Protocol to differentially quantify spatially resolved viral protein-cellular protein interactions via proximity ligation assays

    doi: 10.1016/j.xpro.2026.104361

    Figure Lengend Snippet: Expected outcome of the PLA analysis of an overexpressed viral protein and an endogenous protein (A) Representative images of PLA protocol performed in cells either transfected with a vector control or a vector encoding for StrepII-tagged SARS-CoV-2 E T9 (wildtype) or the T9I variant. Cells are incubated with the individual antibodies targeted against StrepII-tag or the endogenous target ABCG2. Red, PLA; blue, DAPI. Scale bar: 20 μm (B) Quantification of the immunofluorescence images of (A). Lines represent the mean of N = 19-70 (individual cells) ± SEM.

    Article Snippet: Monoclonal mouse anti-ABCG2 Antibody (BXP-21) (PLA 1:100) , Santa Cruz Biotechnology , Cat#sc-58222; RRID: AB_630828.

    Techniques: Transfection, Plasmid Preparation, Control, Variant Assay, Incubation, Immunofluorescence