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atp binding aptamer sequences (aba) 5′ sh-acctgggggagtattgcggaggaaggt-3′  (Sangon Biotech)

 
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    Structured Review

    Sangon Biotech atp binding aptamer sequences (aba) 5′ sh-acctgggggagtattgcggaggaaggt-3′
    Atp Binding Aptamer Sequences (Aba) 5′ Sh Acctgggggagtattgcggaggaaggt 3′, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aba+aptamer+sequence/the+atp+binding+hairpin+aptamer+with+the+following+sequence++5++ctccgcaacctgggggagtattgcggaggaaggttgcgg+3+/pm22876875-36-0-19
    Average 90 stars, based on 1 article reviews
    atp binding aptamer sequences (aba) 5′ sh-acctgggggagtattgcggaggaaggt-3′ - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Two-Dimension (2D) Cu-MOFs/aptamer Nanoprobe for In Situ ATP Imaging in Living Cells
    Article Snippet: Monitoring the intracellular adenosine triphosphate (ATP) level is vital for elaborating its function in physiological states.. However, the intracellular fluorescence sensing of ATP using ATP aptamer remains difficult owing to non-target displacement and susceptibility toward enzymatic degradation.. Herein, by combining 2D Cu-MOF nanosheets and FAM labelled ATP aptamer, we developed a fluorescent Cu-MOFs/aptamer nanoprobe to image and sense intracellular ATP.

    Article Title: Graphene foam field-effect transistor for ultra-sensitive label-free detection of ATP
    Article Snippet: As the major energy molecule of cells, adenosine triphosphate (ATP) regulates various biological processes and has been found to be closely related to many diseases.. Therefore, ATP detection in trace amounts is very useful for understanding various biological processes, studying cellular events such as proliferation and apoptosis, and estimating contaminated degree of food and medical instrument.. To date, the trace sensing ATP at picomolar level in biological systems is still a major challenge.

    Article Title: Label-free Aptasensor based on Electrodeposition of Gold Nanoparticles on Graphene and Its Application in the Quantification of Adenosine Triphosphate
    Article Snippet: A novel and sensitive label-free aptasensor has been developed based on direct electrodeposition of gold nanoparticles (AuNPs) on NH2-reduced graphene oxide (rGo-NH2) modified electrode for the determination of adenosine triphosphate (ATP).. The constructed AuNPs/rGo-NH2 nanocomposite not only has the merits of large specific surface area and good biocompatibility, but also has high conductivity and excellent electrochemical activity.. These deposited AuNPs produced a strong electrochemical signal, which can be used as redox probes for the detection of ATP.

    Article Title: A facile label-free G-quadruplex based fluorescent aptasensor method for rapid detection of ATP.
    Article Snippet: Article history: Received 21 September 2016 Received in revised form 15 December 2016 Accepted 20 December 2016 Available online 21 December 2016 The present work demonstrates a simple, rapid and label-free ATP detection method using a fluorescent aptasensor that is based on G-quadruplex formation.. In the absence of ATP, the Thioflavin T (ThT) dye binds to the G-rich ATP aptamer and forms an ATP aptamer/ThT G-quadruplex complex, which results in high fluorescence intensity.. Upon addition of ATP, the ATP aptamer/ThT complex will be replaced by the formation of an ATP aptamer/ATP complex.

    Article Title: Peroxiredoxin 1 has an anti-apoptotic role via apoptosis signal-regulating kinase 1 and p38 activation in mouse models with oral precancerous lesions.
    Article Snippet: Sequences for all target gene primers were synthesized by Sangon Biotech (Shanghai, China) as follows: Prx1, forward: 5'-AAT GCA AAA ATT GGG TAT CCT GC-3' and reverse 5'-CGT GGG ACA CAC AAA AGT AAA GT-3'; ASK1, forward: 5'-AAG TCC CAA CCC ATA GAA ATT CCT-3' and reverse 5'-AGC CAG TCG GTA AGT TCA GAA TCT T-3'; p38, forward 5'-GAG CTG AAG ATT CTG GAT TTT GG-3' and reverse 5'-TAG CCA CGT AGC CGG TCA TT-3'; glyceraldehyde 3-phosphate dehydrogenase (GAPDH), forward 5'-AGG TCG GTG TGA ACG GAT TTG-3' and reverse 5'-TGT AGA CCA TGA GTT GAG GTC A-3'.

    Article Title: Inhibition of cell proliferation through an ATP-responsive co-delivery system of doxorubicin and Bcl-2 siRNA
    Article Snippet: ATP-responsive aptamer (5′-ACC TGG GGG AGT ATT GCG GAG GAA GGT-3′) and its cDNA (5′-ACC TTC CTC CGC AAT ACT CCC CCA GGT-3′) were synthesized by Sangon Biotech (Shanghai, People’s Republic of China).

    Article Title: An electrochemical aptasensor based on bifunctional Fe3O4@Au nanocomposites for adenosine triphosphate assay
    Article Snippet: We describe a label-free electrochemical aptasensor for adenosine triphosphate (ATP) assay based on bifunctional Fe3O4@Au nanocomposites.. Due to the introduction of Au nanoparticles, the resulting bifunctional Fe3O4@Au nanocomposites can be easily modified with ATP-specific aptamer molecules through Au–S interaction to build the nanoprobe and used to immobilize on a glassy carbon electrode simply with the assistance of a magnet.. Once exposed to the target ATP, the current signal obtained at nanoprobe-containingmagnetic glassy carbon electrode decreases immediately.

    Binding Assay:

    Article Title: Target-induced structure switching of hairpin aptamers for label-free and sensitive fluorescent detection of ATP via exonuclease-catalyzed target recycling amplification.
    Article Snippet: In this work, we described the development of a new label-free, simple and sensitive fluorescent ATP sensing platform based on exonuclease III (Exo III)-catalyzed target recycling (ECTR) amplification and SYBR Green I indicator.. The hairpin aptamer probes underwent conformational structure switching and re-configuration in the presence of ATP, which led to catalytic cleavage of the re-configured aptamers by Exo III to release ATP and to initiate the ECTR process.. Such ECTR process resulted in the digestion of a significant number of the hairpin aptamer probes, leading to much less intercalation of SYBR Green I to the hairpin stems and drastic suppression of the fluorescence emission for sensitive ATP detection down to the low nanomolar level.

    Sequencing:

    Article Title: Target-induced structure switching of hairpin aptamers for label-free and sensitive fluorescent detection of ATP via exonuclease-catalyzed target recycling amplification.
    Article Snippet: In this work, we described the development of a new label-free, simple and sensitive fluorescent ATP sensing platform based on exonuclease III (Exo III)-catalyzed target recycling (ECTR) amplification and SYBR Green I indicator.. The hairpin aptamer probes underwent conformational structure switching and re-configuration in the presence of ATP, which led to catalytic cleavage of the re-configured aptamers by Exo III to release ATP and to initiate the ECTR process.. Such ECTR process resulted in the digestion of a significant number of the hairpin aptamer probes, leading to much less intercalation of SYBR Green I to the hairpin stems and drastic suppression of the fluorescence emission for sensitive ATP detection down to the low nanomolar level.

    Synthesized:

    Article Title: Target-induced structure switching of hairpin aptamers for label-free and sensitive fluorescent detection of ATP via exonuclease-catalyzed target recycling amplification.
    Article Snippet: In this work, we described the development of a new label-free, simple and sensitive fluorescent ATP sensing platform based on exonuclease III (Exo III)-catalyzed target recycling (ECTR) amplification and SYBR Green I indicator.. The hairpin aptamer probes underwent conformational structure switching and re-configuration in the presence of ATP, which led to catalytic cleavage of the re-configured aptamers by Exo III to release ATP and to initiate the ECTR process.. Such ECTR process resulted in the digestion of a significant number of the hairpin aptamer probes, leading to much less intercalation of SYBR Green I to the hairpin stems and drastic suppression of the fluorescence emission for sensitive ATP detection down to the low nanomolar level.

    Purification:

    Article Title: Target-induced structure switching of hairpin aptamers for label-free and sensitive fluorescent detection of ATP via exonuclease-catalyzed target recycling amplification.
    Article Snippet: In this work, we described the development of a new label-free, simple and sensitive fluorescent ATP sensing platform based on exonuclease III (Exo III)-catalyzed target recycling (ECTR) amplification and SYBR Green I indicator.. The hairpin aptamer probes underwent conformational structure switching and re-configuration in the presence of ATP, which led to catalytic cleavage of the re-configured aptamers by Exo III to release ATP and to initiate the ECTR process.. Such ECTR process resulted in the digestion of a significant number of the hairpin aptamer probes, leading to much less intercalation of SYBR Green I to the hairpin stems and drastic suppression of the fluorescence emission for sensitive ATP detection down to the low nanomolar level.



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    Sangon Biotech aba aptamer sequence
    The feasibility verification of the experimental scheme. ( A ) UV-Vis spectra; ( B ) color change of AuNPs of different systems; (a: Au; b: Au + NaCl; c: Au + CV; d: Au + CV + NaCl; e: Au + CV + Apt; f: Au + CV + Apt + NaCl; g: Au + CV + Apt + <t>ABA</t> + NaCl); ( C ) circular dichroism spectra of <t>aptamer</t> before and after binding to ABA; ( D ) SERS spectrum of CV before and after adding sodium chloride.
    Aba Aptamer Sequence, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aba+aptamer+sequence/sequence+specific/pmc13024280-50-1-34
    Average 86 stars, based on 1 article reviews
    aba aptamer sequence - by Bioz Stars, 2026-11
    86/100 stars
      Buy from Supplier

    90
    Sangon Biotech atp binding aptamer sequences (aba) 5′ sh-acctgggggagtattgcggaggaaggt-3′
    The feasibility verification of the experimental scheme. ( A ) UV-Vis spectra; ( B ) color change of AuNPs of different systems; (a: Au; b: Au + NaCl; c: Au + CV; d: Au + CV + NaCl; e: Au + CV + Apt; f: Au + CV + Apt + NaCl; g: Au + CV + Apt + <t>ABA</t> + NaCl); ( C ) circular dichroism spectra of <t>aptamer</t> before and after binding to ABA; ( D ) SERS spectrum of CV before and after adding sodium chloride.
    Atp Binding Aptamer Sequences (Aba) 5′ Sh Acctgggggagtattgcggaggaaggt 3′, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aba+aptamer+sequence/the+atp+binding+hairpin+aptamer+with+the+following+sequence++5++ctccgcaacctgggggagtattgcggaggaaggttgcgg+3+/pm22876875-36-0-19
    Average 90 stars, based on 1 article reviews
    atp binding aptamer sequences (aba) 5′ sh-acctgggggagtattgcggaggaaggt-3′ - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

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    The feasibility verification of the experimental scheme. ( A ) UV-Vis spectra; ( B ) color change of AuNPs of different systems; (a: Au; b: Au + NaCl; c: Au + CV; d: Au + CV + NaCl; e: Au + CV + Apt; f: Au + CV + Apt + NaCl; g: Au + CV + Apt + ABA + NaCl); ( C ) circular dichroism spectra of aptamer before and after binding to ABA; ( D ) SERS spectrum of CV before and after adding sodium chloride.

    Journal: Biosensors

    Article Title: A SERS/LSPR Dual-Signal Aptamer Sensor for Abscisic Acid Detection Based on Unmodified Gold Nanoparticles

    doi: 10.3390/bios16030152

    Figure Lengend Snippet: The feasibility verification of the experimental scheme. ( A ) UV-Vis spectra; ( B ) color change of AuNPs of different systems; (a: Au; b: Au + NaCl; c: Au + CV; d: Au + CV + NaCl; e: Au + CV + Apt; f: Au + CV + Apt + NaCl; g: Au + CV + Apt + ABA + NaCl); ( C ) circular dichroism spectra of aptamer before and after binding to ABA; ( D ) SERS spectrum of CV before and after adding sodium chloride.

    Article Snippet: The ABA aptamer sequence was ATG GGT TAG GTG GAG GTG ATT CCG GGA ATT CGC CCT AAA TAC GAG CAA C oligonucleotide chain composed of 52 bases [ ], which was purchased from Sangon Biotech Co., Ltd., (Shanghai, China).

    Techniques: Circular Dichroism, Binding Assay

    The SERS intensity ( A ) and A620/A528 ( B ) of dual-signal output aptamer sensor for blank, ABA, GA3, VC, Ethylene, and β-Car. The concentration of ABA was 2 µM, while other interfering molecules were 10 µM. Error bars show the standard deviation of three repeated experiments.

    Journal: Biosensors

    Article Title: A SERS/LSPR Dual-Signal Aptamer Sensor for Abscisic Acid Detection Based on Unmodified Gold Nanoparticles

    doi: 10.3390/bios16030152

    Figure Lengend Snippet: The SERS intensity ( A ) and A620/A528 ( B ) of dual-signal output aptamer sensor for blank, ABA, GA3, VC, Ethylene, and β-Car. The concentration of ABA was 2 µM, while other interfering molecules were 10 µM. Error bars show the standard deviation of three repeated experiments.

    Article Snippet: The ABA aptamer sequence was ATG GGT TAG GTG GAG GTG ATT CCG GGA ATT CGC CCT AAA TAC GAG CAA C oligonucleotide chain composed of 52 bases [ ], which was purchased from Sangon Biotech Co., Ltd., (Shanghai, China).

    Techniques: Concentration Assay, Standard Deviation