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mesenchymal stem cell growth kit for adipose and umbilical derived mscs low serum  (ATCC)


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    Structured Review

    ATCC mesenchymal stem cell growth kit for adipose and umbilical derived mscs low serum
    KEY RESOURCES TABLE
    Mesenchymal Stem Cell Growth Kit For Adipose And Umbilical Derived Mscs Low Serum, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab+microarray+express+buffer+kit/pmc09465545-35-0-14?v=ATCC
    Average 95 stars, based on 126 article reviews
    mesenchymal stem cell growth kit for adipose and umbilical derived mscs low serum - by Bioz Stars, 2026-06
    95/100 stars

    Images

    1) Product Images from "The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma"

    Article Title: The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma

    Journal: Molecular cell

    doi: 10.1016/j.molcel.2022.03.019

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Microarray, Recombinant, Sample Purification, Isolation, Derivative Assay, shRNA, Construct, CRISPR, Plasmid Preparation, Software

    A. Experimental schematic to profile BAF complex localization and other chromatin changes in hTERT-MSCs upon 24 hours adipogenesis. B. edgeR analysis of differential occupancy of BAF complexes (SMARCA4) on the genome upon 24 hours adipogenesis. C. HOMER motif analysis of sites that gain BAF complex (SMARCA4) occupancy upon 24 hours adipogenesis. D. Heatmap of SMARCA4, CEBPB, H3K27ac, and ATAC-seq peaks over sites that gain BAF complex (SMARCA4) occupancy upon 24 hours adipogenesis (“SMARCA4 targets”). E. Experimental schematic to define the specific role for BAF complexes in adipogenesis using small molecule-mediated inhibition of BAF complex ATPase activity with compound 12 (CMP12). F. Heatmap of SMARCA4, CEBPB, H3K27ac, and ATAC-seq peaks upon DMSO or CMP12 treatment in base or adipogenic media for 24 hours over SMARCA4 gained sites in the DMSO adipogenesis condition. G. Comparison of changes in RNA in the CMP12 and DMSO treatment conditions upon adipogenesis. Dark blue circles are genes that are not as up-regulated or are more down-regulated upon adipogenesis with CMP12 treatment compared to DMSO (CMP12-attenuated gene). Light blue circles are a subset of CMP12-attenuated genes that are also nearest to sites that gain BAF complexes (SMARCA4) upon adipogenesis in the DMSO condition (CMP12-attenuated gene and SMARCA4 target). H. Gene ontology analysis of CMP12-attenuated gene and SMARCA4 targets. I. Heatmap of logFC in expression of genes in DMSO and CMP12 conditions upon adipogenesis. J. Track examples at the CD36, ELOVL6 and WNT5A loci. See also Figure S4.
    Figure Legend Snippet: A. Experimental schematic to profile BAF complex localization and other chromatin changes in hTERT-MSCs upon 24 hours adipogenesis. B. edgeR analysis of differential occupancy of BAF complexes (SMARCA4) on the genome upon 24 hours adipogenesis. C. HOMER motif analysis of sites that gain BAF complex (SMARCA4) occupancy upon 24 hours adipogenesis. D. Heatmap of SMARCA4, CEBPB, H3K27ac, and ATAC-seq peaks over sites that gain BAF complex (SMARCA4) occupancy upon 24 hours adipogenesis (“SMARCA4 targets”). E. Experimental schematic to define the specific role for BAF complexes in adipogenesis using small molecule-mediated inhibition of BAF complex ATPase activity with compound 12 (CMP12). F. Heatmap of SMARCA4, CEBPB, H3K27ac, and ATAC-seq peaks upon DMSO or CMP12 treatment in base or adipogenic media for 24 hours over SMARCA4 gained sites in the DMSO adipogenesis condition. G. Comparison of changes in RNA in the CMP12 and DMSO treatment conditions upon adipogenesis. Dark blue circles are genes that are not as up-regulated or are more down-regulated upon adipogenesis with CMP12 treatment compared to DMSO (CMP12-attenuated gene). Light blue circles are a subset of CMP12-attenuated genes that are also nearest to sites that gain BAF complexes (SMARCA4) upon adipogenesis in the DMSO condition (CMP12-attenuated gene and SMARCA4 target). H. Gene ontology analysis of CMP12-attenuated gene and SMARCA4 targets. I. Heatmap of logFC in expression of genes in DMSO and CMP12 conditions upon adipogenesis. J. Track examples at the CD36, ELOVL6 and WNT5A loci. See also Figure S4.

    Techniques Used: Inhibition, Activity Assay, Comparison, Expressing

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Microarray, Recombinant, Sample Purification, Isolation, Derivative Assay, shRNA, Construct, CRISPR, Plasmid Preparation, Software



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