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aav shctrl  (Addgene inc)


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    Structured Review

    Addgene inc aav shctrl
    Aav Shctrl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav-shctrl/PX552+(Plasmid+%2360958)/pmc12339988-44-3-4
    Average 95 stars, based on 106 article reviews
    aav shctrl - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Cloning:

    Article Title: Disrupted astrocyte-neuron glutamine-glutamate cycling in the medial prefrontal cortex contributes to depression-like behaviors
    Article Snippet: .. For cloning sgRNA, we first generated the AAV viral vector of serotype 2, called pX552ch, by replacing the coding sequence (CDS) of EGFP of pX552 (Addgene, Watertown, MA, USA) with the CDS of mCherry. .. Various sgRNA sequences were subsequently subcloned into the sgRNA replacement site of pX552ch by using the HiFi assembly method (New England Biolabs, Ipswich, MA, USA), and the resulting plasmid were verified by sequencing.

    Article Title: Disrupted astrocyte-neuron glutamine-glutamate cycling in the medial prefrontal cortex contributes to depression-like behaviors
    Article Snippet: .. Small guide RNA (sgRNA) and adeno-associated virus (AAV) For cloning sgRNA, we first generated the AAV viral vector of serotype 2, called pX552ch, by replacing the coding sequence (CDS) of EGFP of pX552 (Addgene, Watertown, MA, USA) with the CDS of mCherry. .. Various sgRNA sequences were subsequently subcloned into the sgRNA replacement site of pX552ch by using the HiFi assembly method (New England Biolabs, Ipswich, MA, USA), and the resulting plasmid were verified by sequencing.

    Generated:

    Article Title: Disrupted astrocyte-neuron glutamine-glutamate cycling in the medial prefrontal cortex contributes to depression-like behaviors
    Article Snippet: .. For cloning sgRNA, we first generated the AAV viral vector of serotype 2, called pX552ch, by replacing the coding sequence (CDS) of EGFP of pX552 (Addgene, Watertown, MA, USA) with the CDS of mCherry. .. Various sgRNA sequences were subsequently subcloned into the sgRNA replacement site of pX552ch by using the HiFi assembly method (New England Biolabs, Ipswich, MA, USA), and the resulting plasmid were verified by sequencing.

    Article Title: A systematic cross-modal approach identifies astrocytic VCAM1 as a regulator of hippocampal synapse development
    Article Snippet: .. An AAV backbone vector was generated using pAAV-U6-sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor; Addgene #60958) and AAV pCAG-FLEX-tdTomato-WPRE (Addgene #51503). ..

    Article Title: Disrupted astrocyte-neuron glutamine-glutamate cycling in the medial prefrontal cortex contributes to depression-like behaviors
    Article Snippet: .. Small guide RNA (sgRNA) and adeno-associated virus (AAV) For cloning sgRNA, we first generated the AAV viral vector of serotype 2, called pX552ch, by replacing the coding sequence (CDS) of EGFP of pX552 (Addgene, Watertown, MA, USA) with the CDS of mCherry. .. Various sgRNA sequences were subsequently subcloned into the sgRNA replacement site of pX552ch by using the HiFi assembly method (New England Biolabs, Ipswich, MA, USA), and the resulting plasmid were verified by sequencing.

    Bioprocessing:

    Article Title: Disrupted astrocyte-neuron glutamine-glutamate cycling in the medial prefrontal cortex contributes to depression-like behaviors
    Article Snippet: .. For cloning sgRNA, we first generated the AAV viral vector of serotype 2, called pX552ch, by replacing the coding sequence (CDS) of EGFP of pX552 (Addgene, Watertown, MA, USA) with the CDS of mCherry. .. Various sgRNA sequences were subsequently subcloned into the sgRNA replacement site of pX552ch by using the HiFi assembly method (New England Biolabs, Ipswich, MA, USA), and the resulting plasmid were verified by sequencing.

    Article Title: A systematic cross-modal approach identifies astrocytic VCAM1 as a regulator of hippocampal synapse development
    Article Snippet: .. An AAV backbone vector was generated using pAAV-U6-sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor; Addgene #60958) and AAV pCAG-FLEX-tdTomato-WPRE (Addgene #51503). ..

    Article Title: Disrupted astrocyte-neuron glutamine-glutamate cycling in the medial prefrontal cortex contributes to depression-like behaviors
    Article Snippet: .. Small guide RNA (sgRNA) and adeno-associated virus (AAV) For cloning sgRNA, we first generated the AAV viral vector of serotype 2, called pX552ch, by replacing the coding sequence (CDS) of EGFP of pX552 (Addgene, Watertown, MA, USA) with the CDS of mCherry. .. Various sgRNA sequences were subsequently subcloned into the sgRNA replacement site of pX552ch by using the HiFi assembly method (New England Biolabs, Ipswich, MA, USA), and the resulting plasmid were verified by sequencing.

    Plasmid Preparation:

    Article Title: Disrupted astrocyte-neuron glutamine-glutamate cycling in the medial prefrontal cortex contributes to depression-like behaviors
    Article Snippet: .. For cloning sgRNA, we first generated the AAV viral vector of serotype 2, called pX552ch, by replacing the coding sequence (CDS) of EGFP of pX552 (Addgene, Watertown, MA, USA) with the CDS of mCherry. .. Various sgRNA sequences were subsequently subcloned into the sgRNA replacement site of pX552ch by using the HiFi assembly method (New England Biolabs, Ipswich, MA, USA), and the resulting plasmid were verified by sequencing.

    Article Title: Spatial proteomics reveals prefrontal circuit diversity in socioemotional behaviour
    Article Snippet: The shRNA sequences used were as follows: shCont, 5′-GATCACGGATTCGAGAACAGA-3′; shBLTP2#1, 5′-GGATCCAGAATGTCAGTCTT-3′; shBLTP2#2, 5′-GCCATGATCTGCCAAACTAT-3′. pAAV-TIWB-myrmScarlet-I-P2A-post-eGRASP (Addgene plasmid #111584), pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP (Addgene plasmid #111585), pAAV-EWB-DIO-cyan pre-eGRASP(p32) (Addgene plasmid #111589), pAAV-TIWB-yellow pre-eGRASP(p30) (Addgene plasmid #111588) and pAAV-FAH-rtTA3G (Addgene plasmid #120309) were gifts from Bong-Kiun Kaang. pCAG-HA-Nrxn1alpha was a gift from Peter Scheiffele (Addgene plasmid #58266). .. NGL-1-myc/pEGFP-N1 was a gift from Eunjoon Kim (Addgene plasmid # 197314). pAAV-EF1a-DIO-hChR2(H134R)-P2A-EYFP was a gift from Mingshan Xue (Addgene plasmid #139283). pAAV-pMecp2-SpCas9-spA (PX551) (Addgene plasmid #60957) and PX552 (pAAV-U-6sgRNA (SapI)_hSyn-GFP-KASH-bGH) (Addgene plasmid #60958) were gifts from Feng Zhang. ..

    Article Title: Integrating traditional omics and AI-driven approaches for discovery and validation of novel MicroRNA biomarkers and therapeutic targets in thyroid cancer
    Article Snippet: The lentiviral vector pLV-EF1α-dCas9-KRAB-T2A-Puro (Addgene plasmid number 71236, Addgene, Watertown, MA, USA) was used to express the dCas9-KRAB fusion protein. .. Guide RNAs targeting the MIR6756 promoter region were designed using the CHOPCHOP web tool ( http://chopchop.cbu.uib.no/ ) and cloned into the pLKO.1-U6-sgRNA-EF1α-Puro vector (Addgene plasmid number 60958). ..

    Article Title: Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons.
    Article Snippet: .. Use a CRISPR-Cas9 knock-in (KI) vector based on the PX552 plasmid backbone (Addgene #60958) to insert a fluorescent tag at the Snca locus. b. ..

    Article Title: A systematic cross-modal approach identifies astrocytic VCAM1 as a regulator of hippocampal synapse development
    Article Snippet: .. An AAV backbone vector was generated using pAAV-U6-sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor; Addgene #60958) and AAV pCAG-FLEX-tdTomato-WPRE (Addgene #51503). ..

    Article Title: Disrupted astrocyte-neuron glutamine-glutamate cycling in the medial prefrontal cortex contributes to depression-like behaviors
    Article Snippet: .. Small guide RNA (sgRNA) and adeno-associated virus (AAV) For cloning sgRNA, we first generated the AAV viral vector of serotype 2, called pX552ch, by replacing the coding sequence (CDS) of EGFP of pX552 (Addgene, Watertown, MA, USA) with the CDS of mCherry. .. Various sgRNA sequences were subsequently subcloned into the sgRNA replacement site of pX552ch by using the HiFi assembly method (New England Biolabs, Ipswich, MA, USA), and the resulting plasmid were verified by sequencing.

    Article Title: Photomapping electrically coupled networks of the mature thalamus and cortex.
    Article Snippet: .. Briefly, the PX552 plasmid (Addgene, plasmid no. 60958) was modified to encode triple U6sgRNA cassettes each targeting a distinct locus of the target genes. ..

    Sequencing:

    Article Title: Disrupted astrocyte-neuron glutamine-glutamate cycling in the medial prefrontal cortex contributes to depression-like behaviors
    Article Snippet: .. For cloning sgRNA, we first generated the AAV viral vector of serotype 2, called pX552ch, by replacing the coding sequence (CDS) of EGFP of pX552 (Addgene, Watertown, MA, USA) with the CDS of mCherry. .. Various sgRNA sequences were subsequently subcloned into the sgRNA replacement site of pX552ch by using the HiFi assembly method (New England Biolabs, Ipswich, MA, USA), and the resulting plasmid were verified by sequencing.

    Article Title: Disrupted astrocyte-neuron glutamine-glutamate cycling in the medial prefrontal cortex contributes to depression-like behaviors
    Article Snippet: .. Small guide RNA (sgRNA) and adeno-associated virus (AAV) For cloning sgRNA, we first generated the AAV viral vector of serotype 2, called pX552ch, by replacing the coding sequence (CDS) of EGFP of pX552 (Addgene, Watertown, MA, USA) with the CDS of mCherry. .. Various sgRNA sequences were subsequently subcloned into the sgRNA replacement site of pX552ch by using the HiFi assembly method (New England Biolabs, Ipswich, MA, USA), and the resulting plasmid were verified by sequencing.

    Clone Assay:

    Article Title: Integrating traditional omics and AI-driven approaches for discovery and validation of novel MicroRNA biomarkers and therapeutic targets in thyroid cancer
    Article Snippet: The lentiviral vector pLV-EF1α-dCas9-KRAB-T2A-Puro (Addgene plasmid number 71236, Addgene, Watertown, MA, USA) was used to express the dCas9-KRAB fusion protein. .. Guide RNAs targeting the MIR6756 promoter region were designed using the CHOPCHOP web tool ( http://chopchop.cbu.uib.no/ ) and cloned into the pLKO.1-U6-sgRNA-EF1α-Puro vector (Addgene plasmid number 60958). ..

    CRISPR:

    Article Title: Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons.
    Article Snippet: .. Use a CRISPR-Cas9 knock-in (KI) vector based on the PX552 plasmid backbone (Addgene #60958) to insert a fluorescent tag at the Snca locus. b. ..

    Knock-In:

    Article Title: Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons.
    Article Snippet: .. Use a CRISPR-Cas9 knock-in (KI) vector based on the PX552 plasmid backbone (Addgene #60958) to insert a fluorescent tag at the Snca locus. b. ..

    Virus:

    Article Title: Disrupted astrocyte-neuron glutamine-glutamate cycling in the medial prefrontal cortex contributes to depression-like behaviors
    Article Snippet: .. Small guide RNA (sgRNA) and adeno-associated virus (AAV) For cloning sgRNA, we first generated the AAV viral vector of serotype 2, called pX552ch, by replacing the coding sequence (CDS) of EGFP of pX552 (Addgene, Watertown, MA, USA) with the CDS of mCherry. .. Various sgRNA sequences were subsequently subcloned into the sgRNA replacement site of pX552ch by using the HiFi assembly method (New England Biolabs, Ipswich, MA, USA), and the resulting plasmid were verified by sequencing.

    Modification:

    Article Title: Photomapping electrically coupled networks of the mature thalamus and cortex.
    Article Snippet: .. Briefly, the PX552 plasmid (Addgene, plasmid no. 60958) was modified to encode triple U6sgRNA cassettes each targeting a distinct locus of the target genes. ..



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