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aav9 cag flex tdtomato wpre bgh  (Addgene inc)


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    Addgene inc aav9 cag flex tdtomato wpre bgh
    Aav9 Cag Flex Tdtomato Wpre Bgh, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav+flex/AAV+pCAG-FLEX-tdTomato-WPRE+(Plasmid+%2351503)/pm41906363-69-0-1
    Average 93 stars, based on 60 article reviews
    aav9 cag flex tdtomato wpre bgh - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: SeeThrough: a rationally designed skull clearing technique for in vivo brain imaging
    Article Snippet: Custom AAV vectors were produced by Addgene, Penn Vector Core at the University of Pennsylvania, or Virus Vector Core at the program for Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS). .. AAV-CAG-FLEX-tdTomato (Addgene, #51503), AAV-Syn-Cre (Brain/MINDS, #FA-022), and AAV-HDR-mEGFP-CaMKIIα were pseudotyped with serotype 1. ..

    Article Title: SeeThrough: a rationally designed skull clearing technique for in vivo brain imaging.
    Article Snippet: Custom AAV vectors were produced by Addgene, Penn Vector Core at the University of Pennsylvania, or Virus Vector Core at the program for Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS). .. AAV-CAG-FLEX-tdTomato (Addgene, #51503), AAVSyn-Cre (Brain/MINDS, #FA-022), and AAV-HDR-mEGFP-CaMKIIα18 were pseudotypedwith serotype 1. ..

    Article Title: A systematic cross-modal approach identifies astrocytic VCAM1 as a regulator of hippocampal synapse development
    Article Snippet: .. An AAV backbone vector was generated using pAAV-U6-sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor; Addgene #60958) and AAV pCAG-FLEX-tdTomato-WPRE (Addgene #51503). ..

    Injection:

    Article Title: Hippocampal input-driven plasticity of prefrontal interneurons reveals a circuit basis for impaired spatial working memory
    Article Snippet: AAV1.Syn::ChrimsonR-tdTomato (Addgene, 59171, 5x10 GC/mL) was injected into vHPC (D/V: -3.25, 600nL). .. A combination AAV9.CAG::FLEX.tdTomato (Addgene, 51503) and AAV9.EF1a::fDIO.EYFP (Vector Biolabs) with final titers of 1.7x10 and 1.25x10 GC/mL, respectively, was injected into mPFC (D/V: -1.45, 500 nL). ..

    Article Title: Basal forebrain cholinergic input mediates adaptive attention allocation to enhance olfactory discrimination
    Article Snippet: Excitatory DREADDs were bilaterally expressed in the HDB ChAT cells (AAV2-DIO-hM3D(Gq)-mCherry (Addgene, 44361-AAV2) in ChAT-Cre mice. .. Control experiments were conducted on DAT-Cre mice injected with AAV1-CAG-LSL-tdTomato (Addgene, 100048-AAV1) and ChAT-Cre mice injected with AAVrg-CAG-FLEX-tdTomato (Addgene, 51503-AAVrg) in the OB and AAV1-CAG-LSL-tdTomato in the HDB. .. Clozapine N-oxide (CNO, Abcam, ab141704) was diluted in 1× PBS and injected intraperitoneally at a dose of 2.5 mg/kg, 20 min before all behavior and imaging experiments.

    Article Title: Rapid motor skill adjustment is associated with population-level modulation of cerebellar error signals.
    Article Snippet: A core principle of cerebellar learning theories is that climbing fibers from the inferior olive convey error signals about movement execution to Purkinje cells in the cerebellar cortex.. These inputs trigger synaptic changes, which are purported to drive progressive adjustment of future movements.. Individually, binary complex spike signals lack information about the sign and magnitude of errors which presents a problem for cerebellar learning paradigms exhibiting fast adaptation.

    Article Title: Dopaminergic short axon cells integrate sensory and top–down inputs to enhance discriminative learning in the mouse olfactory bulb
    Article Snippet: Inhibitory DREADDs were expressed bilaterally to infect SACs using AAV2-hSyn-DIO-hM4DGi-mCherry (Addgene, 44362-AAV2) in DAT-Cre mice. .. Control experiments involved DAT-Cre mice injected with AAV1-CAG-LSL-tdTomato (Addgene, 100048-AAV1) and ChAT-Cre mice injected with AAVrg-CAG-FLEX-tdTomato (Addgene, 51503-AAVrg) in the olfactory bulb, as well as AAV1-CAG-LSL-tdTomato in the HDB. .. Clozapine N-oxide (CNO, Abcam, ab141704) was prepared in 1× PBS and administered intraperitoneally at a dose of 2.5 mg/kg, 20 min prior to all behavioral and imaging experiments.

    Control:

    Article Title: Basal forebrain cholinergic input mediates adaptive attention allocation to enhance olfactory discrimination
    Article Snippet: Excitatory DREADDs were bilaterally expressed in the HDB ChAT cells (AAV2-DIO-hM3D(Gq)-mCherry (Addgene, 44361-AAV2) in ChAT-Cre mice. .. Control experiments were conducted on DAT-Cre mice injected with AAV1-CAG-LSL-tdTomato (Addgene, 100048-AAV1) and ChAT-Cre mice injected with AAVrg-CAG-FLEX-tdTomato (Addgene, 51503-AAVrg) in the OB and AAV1-CAG-LSL-tdTomato in the HDB. .. Clozapine N-oxide (CNO, Abcam, ab141704) was diluted in 1× PBS and injected intraperitoneally at a dose of 2.5 mg/kg, 20 min before all behavior and imaging experiments.

    Article Title: Dopaminergic short axon cells integrate sensory and top–down inputs to enhance discriminative learning in the mouse olfactory bulb
    Article Snippet: Inhibitory DREADDs were expressed bilaterally to infect SACs using AAV2-hSyn-DIO-hM4DGi-mCherry (Addgene, 44362-AAV2) in DAT-Cre mice. .. Control experiments involved DAT-Cre mice injected with AAV1-CAG-LSL-tdTomato (Addgene, 100048-AAV1) and ChAT-Cre mice injected with AAVrg-CAG-FLEX-tdTomato (Addgene, 51503-AAVrg) in the olfactory bulb, as well as AAV1-CAG-LSL-tdTomato in the HDB. .. Clozapine N-oxide (CNO, Abcam, ab141704) was prepared in 1× PBS and administered intraperitoneally at a dose of 2.5 mg/kg, 20 min prior to all behavioral and imaging experiments.

    Article Title: Cocaine-Induced Immediate-Early Gene Expression in the Nucleus Accumbens: Roles of Separate cAMP Sensors.
    Article Snippet: Immediateearly gene (IEG) induction guides elucidation of signaling pathways mediating neuronal plasticity underlying compulsive use of psychostimulants.. IEG induction after psychostimulant administration has been attributed to both PKAand RapGEF2dependent signaling pathways initiated by D1 receptor stimulation by dopamine.. However, it is not clear how each pathway contributes individually to IEG induction, dopaminoceptive neuronal activity, and neuronal plasticity.

    Plasmid Preparation:

    Article Title: A systematic cross-modal approach identifies astrocytic VCAM1 as a regulator of hippocampal synapse development
    Article Snippet: .. An AAV backbone vector was generated using pAAV-U6-sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor; Addgene #60958) and AAV pCAG-FLEX-tdTomato-WPRE (Addgene #51503). ..

    Article Title: Rapid motor skill adjustment is associated with population-level modulation of cerebellar error signals.
    Article Snippet: A core principle of cerebellar learning theories is that climbing fibers from the inferior olive convey error signals about movement execution to Purkinje cells in the cerebellar cortex.. These inputs trigger synaptic changes, which are purported to drive progressive adjustment of future movements.. Individually, binary complex spike signals lack information about the sign and magnitude of errors which presents a problem for cerebellar learning paradigms exhibiting fast adaptation.

    Generated:

    Article Title: A systematic cross-modal approach identifies astrocytic VCAM1 as a regulator of hippocampal synapse development
    Article Snippet: .. An AAV backbone vector was generated using pAAV-U6-sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor; Addgene #60958) and AAV pCAG-FLEX-tdTomato-WPRE (Addgene #51503). ..

    Fluorescence:

    Article Title: Rapid motor skill adjustment is associated with population-level modulation of cerebellar error signals.
    Article Snippet: A core principle of cerebellar learning theories is that climbing fibers from the inferior olive convey error signals about movement execution to Purkinje cells in the cerebellar cortex.. These inputs trigger synaptic changes, which are purported to drive progressive adjustment of future movements.. Individually, binary complex spike signals lack information about the sign and magnitude of errors which presents a problem for cerebellar learning paradigms exhibiting fast adaptation.

    Olfactory:

    Article Title: Dopaminergic short axon cells integrate sensory and top–down inputs to enhance discriminative learning in the mouse olfactory bulb
    Article Snippet: Inhibitory DREADDs were expressed bilaterally to infect SACs using AAV2-hSyn-DIO-hM4DGi-mCherry (Addgene, 44362-AAV2) in DAT-Cre mice. .. Control experiments involved DAT-Cre mice injected with AAV1-CAG-LSL-tdTomato (Addgene, 100048-AAV1) and ChAT-Cre mice injected with AAVrg-CAG-FLEX-tdTomato (Addgene, 51503-AAVrg) in the olfactory bulb, as well as AAV1-CAG-LSL-tdTomato in the HDB. .. Clozapine N-oxide (CNO, Abcam, ab141704) was prepared in 1× PBS and administered intraperitoneally at a dose of 2.5 mg/kg, 20 min prior to all behavioral and imaging experiments.

    Virus:

    Article Title: Cocaine-Induced Immediate-Early Gene Expression in the Nucleus Accumbens: Roles of Separate cAMP Sensors.
    Article Snippet: Immediateearly gene (IEG) induction guides elucidation of signaling pathways mediating neuronal plasticity underlying compulsive use of psychostimulants.. IEG induction after psychostimulant administration has been attributed to both PKAand RapGEF2dependent signaling pathways initiated by D1 receptor stimulation by dopamine.. However, it is not clear how each pathway contributes individually to IEG induction, dopaminoceptive neuronal activity, and neuronal plasticity.



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    BNST–PVN-RVLM neural signaling mediates IL-1β–induced changes in heart rate and IL-6. (A) Schematic for anterograde tracing of axonal projections and terminals from PBS-TRAPed or IL-1β–TRAPed BNST <t>neurons.</t> <t>The</t> <t>AAV-hSyn-FLEx-mGFP-2A-synaptophysin-mRuby</t> was injected into the BNST of TRAP2 mice. (B) Representative image for GFP + axons and mRuby + terminals in the PVN of PBS-TRAPed or IL-1β–TRAPed mice. The rightmost panel shows a higher-magnification view of the PVN in IL-1β–TRAPed mice. Arrowheads show regions with co-localization of mRuby and EYFP. Scale bars, 100 μm. (C) Schematic for anterograde tracing of IL-1β–TRAPed BNST neurons connected with PVN neurons. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-Ef1a-fDIO-EYFP was injected into the PVN of TRAP2 mice. (D) Representative image for EYFP expression in the PVN, RVLM, and NTS. Arrowheads show neurons, and arrows show axonal projections with expression of EYFP. Scale bar, 100 μm for the PVN and RVLM. Scale bar, 200 μm for the NTS. (E) c-Fos expression in the RVLM after reactivation with saline as a control or CNO of IL-1β–responsive BNST neurons. Scale bar, 100 μm. (F) Schematic for activating the BNST–PVN neural pathway. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA was injected into the PVN of TRAP2 mice. (G) Representative image of PVN showing Gq-DREADD-mCherry–expressing cells (red). Scale bar, 100 μm. (H) Serum IL-6 levels at 2 h after reactivation with saline as a control or CNO of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (I) ΔHR for 60 min after reactivation of the BNST–PVN neuronal pathway: saline (black) or CNO (red) (saline, n = 7 mice; CNO, n = 10 mice, mixed-effects analysis with Šidák correction). (J) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (K) Serum corticosterone levels at 2 h after reactivation of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. *P < 0.05; **P < 0.01.
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    BNST–PVN-RVLM neural signaling mediates IL-1β–induced changes in heart rate and IL-6. (A) Schematic for anterograde tracing of axonal projections and terminals from PBS-TRAPed or IL-1β–TRAPed BNST <t>neurons.</t> <t>The</t> <t>AAV-hSyn-FLEx-mGFP-2A-synaptophysin-mRuby</t> was injected into the BNST of TRAP2 mice. (B) Representative image for GFP + axons and mRuby + terminals in the PVN of PBS-TRAPed or IL-1β–TRAPed mice. The rightmost panel shows a higher-magnification view of the PVN in IL-1β–TRAPed mice. Arrowheads show regions with co-localization of mRuby and EYFP. Scale bars, 100 μm. (C) Schematic for anterograde tracing of IL-1β–TRAPed BNST neurons connected with PVN neurons. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-Ef1a-fDIO-EYFP was injected into the PVN of TRAP2 mice. (D) Representative image for EYFP expression in the PVN, RVLM, and NTS. Arrowheads show neurons, and arrows show axonal projections with expression of EYFP. Scale bar, 100 μm for the PVN and RVLM. Scale bar, 200 μm for the NTS. (E) c-Fos expression in the RVLM after reactivation with saline as a control or CNO of IL-1β–responsive BNST neurons. Scale bar, 100 μm. (F) Schematic for activating the BNST–PVN neural pathway. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA was injected into the PVN of TRAP2 mice. (G) Representative image of PVN showing Gq-DREADD-mCherry–expressing cells (red). Scale bar, 100 μm. (H) Serum IL-6 levels at 2 h after reactivation with saline as a control or CNO of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (I) ΔHR for 60 min after reactivation of the BNST–PVN neuronal pathway: saline (black) or CNO (red) (saline, n = 7 mice; CNO, n = 10 mice, mixed-effects analysis with Šidák correction). (J) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (K) Serum corticosterone levels at 2 h after reactivation of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. *P < 0.05; **P < 0.01.
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    BNST–PVN-RVLM neural signaling mediates IL-1β–induced changes in heart rate and IL-6. (A) Schematic for anterograde tracing of axonal projections and terminals from PBS-TRAPed or IL-1β–TRAPed BNST <t>neurons.</t> <t>The</t> <t>AAV-hSyn-FLEx-mGFP-2A-synaptophysin-mRuby</t> was injected into the BNST of TRAP2 mice. (B) Representative image for GFP + axons and mRuby + terminals in the PVN of PBS-TRAPed or IL-1β–TRAPed mice. The rightmost panel shows a higher-magnification view of the PVN in IL-1β–TRAPed mice. Arrowheads show regions with co-localization of mRuby and EYFP. Scale bars, 100 μm. (C) Schematic for anterograde tracing of IL-1β–TRAPed BNST neurons connected with PVN neurons. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-Ef1a-fDIO-EYFP was injected into the PVN of TRAP2 mice. (D) Representative image for EYFP expression in the PVN, RVLM, and NTS. Arrowheads show neurons, and arrows show axonal projections with expression of EYFP. Scale bar, 100 μm for the PVN and RVLM. Scale bar, 200 μm for the NTS. (E) c-Fos expression in the RVLM after reactivation with saline as a control or CNO of IL-1β–responsive BNST neurons. Scale bar, 100 μm. (F) Schematic for activating the BNST–PVN neural pathway. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA was injected into the PVN of TRAP2 mice. (G) Representative image of PVN showing Gq-DREADD-mCherry–expressing cells (red). Scale bar, 100 μm. (H) Serum IL-6 levels at 2 h after reactivation with saline as a control or CNO of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (I) ΔHR for 60 min after reactivation of the BNST–PVN neuronal pathway: saline (black) or CNO (red) (saline, n = 7 mice; CNO, n = 10 mice, mixed-effects analysis with Šidák correction). (J) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (K) Serum corticosterone levels at 2 h after reactivation of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. *P < 0.05; **P < 0.01.
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    BNST–PVN-RVLM neural signaling mediates IL-1β–induced changes in heart rate and IL-6. (A) Schematic for anterograde tracing of axonal projections and terminals from PBS-TRAPed or IL-1β–TRAPed BNST <t>neurons.</t> <t>The</t> <t>AAV-hSyn-FLEx-mGFP-2A-synaptophysin-mRuby</t> was injected into the BNST of TRAP2 mice. (B) Representative image for GFP + axons and mRuby + terminals in the PVN of PBS-TRAPed or IL-1β–TRAPed mice. The rightmost panel shows a higher-magnification view of the PVN in IL-1β–TRAPed mice. Arrowheads show regions with co-localization of mRuby and EYFP. Scale bars, 100 μm. (C) Schematic for anterograde tracing of IL-1β–TRAPed BNST neurons connected with PVN neurons. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-Ef1a-fDIO-EYFP was injected into the PVN of TRAP2 mice. (D) Representative image for EYFP expression in the PVN, RVLM, and NTS. Arrowheads show neurons, and arrows show axonal projections with expression of EYFP. Scale bar, 100 μm for the PVN and RVLM. Scale bar, 200 μm for the NTS. (E) c-Fos expression in the RVLM after reactivation with saline as a control or CNO of IL-1β–responsive BNST neurons. Scale bar, 100 μm. (F) Schematic for activating the BNST–PVN neural pathway. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA was injected into the PVN of TRAP2 mice. (G) Representative image of PVN showing Gq-DREADD-mCherry–expressing cells (red). Scale bar, 100 μm. (H) Serum IL-6 levels at 2 h after reactivation with saline as a control or CNO of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (I) ΔHR for 60 min after reactivation of the BNST–PVN neuronal pathway: saline (black) or CNO (red) (saline, n = 7 mice; CNO, n = 10 mice, mixed-effects analysis with Šidák correction). (J) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (K) Serum corticosterone levels at 2 h after reactivation of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. *P < 0.05; **P < 0.01.
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    Image Search Results


    BNST–PVN-RVLM neural signaling mediates IL-1β–induced changes in heart rate and IL-6. (A) Schematic for anterograde tracing of axonal projections and terminals from PBS-TRAPed or IL-1β–TRAPed BNST neurons. The AAV-hSyn-FLEx-mGFP-2A-synaptophysin-mRuby was injected into the BNST of TRAP2 mice. (B) Representative image for GFP + axons and mRuby + terminals in the PVN of PBS-TRAPed or IL-1β–TRAPed mice. The rightmost panel shows a higher-magnification view of the PVN in IL-1β–TRAPed mice. Arrowheads show regions with co-localization of mRuby and EYFP. Scale bars, 100 μm. (C) Schematic for anterograde tracing of IL-1β–TRAPed BNST neurons connected with PVN neurons. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-Ef1a-fDIO-EYFP was injected into the PVN of TRAP2 mice. (D) Representative image for EYFP expression in the PVN, RVLM, and NTS. Arrowheads show neurons, and arrows show axonal projections with expression of EYFP. Scale bar, 100 μm for the PVN and RVLM. Scale bar, 200 μm for the NTS. (E) c-Fos expression in the RVLM after reactivation with saline as a control or CNO of IL-1β–responsive BNST neurons. Scale bar, 100 μm. (F) Schematic for activating the BNST–PVN neural pathway. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA was injected into the PVN of TRAP2 mice. (G) Representative image of PVN showing Gq-DREADD-mCherry–expressing cells (red). Scale bar, 100 μm. (H) Serum IL-6 levels at 2 h after reactivation with saline as a control or CNO of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (I) ΔHR for 60 min after reactivation of the BNST–PVN neuronal pathway: saline (black) or CNO (red) (saline, n = 7 mice; CNO, n = 10 mice, mixed-effects analysis with Šidák correction). (J) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (K) Serum corticosterone levels at 2 h after reactivation of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. *P < 0.05; **P < 0.01.

    Journal: The Journal of Experimental Medicine

    Article Title: Central neurons encode interleukin-1β signals and mediate stress-induced inflammation

    doi: 10.1084/jem.20252000

    Figure Lengend Snippet: BNST–PVN-RVLM neural signaling mediates IL-1β–induced changes in heart rate and IL-6. (A) Schematic for anterograde tracing of axonal projections and terminals from PBS-TRAPed or IL-1β–TRAPed BNST neurons. The AAV-hSyn-FLEx-mGFP-2A-synaptophysin-mRuby was injected into the BNST of TRAP2 mice. (B) Representative image for GFP + axons and mRuby + terminals in the PVN of PBS-TRAPed or IL-1β–TRAPed mice. The rightmost panel shows a higher-magnification view of the PVN in IL-1β–TRAPed mice. Arrowheads show regions with co-localization of mRuby and EYFP. Scale bars, 100 μm. (C) Schematic for anterograde tracing of IL-1β–TRAPed BNST neurons connected with PVN neurons. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-Ef1a-fDIO-EYFP was injected into the PVN of TRAP2 mice. (D) Representative image for EYFP expression in the PVN, RVLM, and NTS. Arrowheads show neurons, and arrows show axonal projections with expression of EYFP. Scale bar, 100 μm for the PVN and RVLM. Scale bar, 200 μm for the NTS. (E) c-Fos expression in the RVLM after reactivation with saline as a control or CNO of IL-1β–responsive BNST neurons. Scale bar, 100 μm. (F) Schematic for activating the BNST–PVN neural pathway. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA was injected into the PVN of TRAP2 mice. (G) Representative image of PVN showing Gq-DREADD-mCherry–expressing cells (red). Scale bar, 100 μm. (H) Serum IL-6 levels at 2 h after reactivation with saline as a control or CNO of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (I) ΔHR for 60 min after reactivation of the BNST–PVN neuronal pathway: saline (black) or CNO (red) (saline, n = 7 mice; CNO, n = 10 mice, mixed-effects analysis with Šidák correction). (J) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (K) Serum corticosterone levels at 2 h after reactivation of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. *P < 0.05; **P < 0.01.

    Article Snippet: For the tracing studies, either AAV-hSyn-DIO-EGFP (cat #50457; Addgene), AAV-hSyn-FLEx-mGFP-2A-Synaptophysin-mRuby (cat# 71760; Addgene), AAV-Ef1a-fDIO-EYFP (cat# 55641; Addgene), or AAV pEF1a-DIO-FLPo-WPRE-hGHpA (cat# 87306; Addgene) was utilized.

    Techniques: Anterograde Tracing, Injection, Expressing, Saline, Control