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Incyte corporation cdna microarray experiment
Cdna Microarray Experiment, supplied by Incyte corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Rat+DNA+Microarrays/cdna+microarray/pm15520318-40-5-12
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cdna microarray experiment - by Bioz Stars, 2026-11
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Related Articles

Microarray:

Article Title: High-Throughput Transcriptome Profiling in Drug and Biomarker Discovery
Article Snippet: .. They used cDNA microarray tools (Incyte, Inc., MO, U.S.A.) to identify the candidate genes responsible for enhancing memory and evaluate the specific gene expression patterns by real-time PCR in SD rat model of passive avoidance task. ..

Article Title: ASBMR 23nd annual meeting.
Article Snippet: .. In this study, cDNA microarray analysis was conducted by Incyte Genomic Systems on 8,734 gene accessions using mRNA from mouse femurs (free of marrow) of both the congenic and B6 strains to test two hypotheses: 1) microarray analysis will identify candidate genes for bone density; and 2) by comparing the gene expression profile outside of the QTL region between B6 and the congenic mice, we can better characterize the molecular phenotypic mechanisms that contribute to the high bone density QTL. ..

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Article Title: Differentially expressed genes in large granular lymphocyte leukemia
Article Snippet: .. Overexpression of a variety cytotoxic genes was observed in leukemic LGL utilizing cDNA microarray from Incyte Genomics (FIG. 1 and Table 4). .. To verify the identity of these overexpressed genes, clones containing cDNA fragments of the selected genes were obtained from Incyte Genomics and confirmed by sequencing.

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..

Article Title: Ectonucleoside triphosphate diphosphohydrolase 6 expression in testis and testicular cancer and its implication in cisplatin resistance.
Article Snippet: After 48 h, the surviving cells were stained with the alamarBlue assay (treK Diagnostic systems, cleveland, oH, UsA) for 180 min at 37°c. the absorbances of the wells were measured using a plate reader (ArVoTM MX; perkin elmer Inc., Waltham, MA, UsA). cDNA microarray analysis. the procedure of cDnA microarray analysis was performed as described previously (18). .. Briefly, cDNA generation, hybridization, and data collection for cDnA microarray analyses were performed by the Incyte corporation (palo Alto, cA, UsA). ..

Gene Expression:

Article Title: High-Throughput Transcriptome Profiling in Drug and Biomarker Discovery
Article Snippet: .. They used cDNA microarray tools (Incyte, Inc., MO, U.S.A.) to identify the candidate genes responsible for enhancing memory and evaluate the specific gene expression patterns by real-time PCR in SD rat model of passive avoidance task. ..

Article Title: ASBMR 23nd annual meeting.
Article Snippet: .. In this study, cDNA microarray analysis was conducted by Incyte Genomic Systems on 8,734 gene accessions using mRNA from mouse femurs (free of marrow) of both the congenic and B6 strains to test two hypotheses: 1) microarray analysis will identify candidate genes for bone density; and 2) by comparing the gene expression profile outside of the QTL region between B6 and the congenic mice, we can better characterize the molecular phenotypic mechanisms that contribute to the high bone density QTL. ..

Real-time Polymerase Chain Reaction:

Article Title: High-Throughput Transcriptome Profiling in Drug and Biomarker Discovery
Article Snippet: .. They used cDNA microarray tools (Incyte, Inc., MO, U.S.A.) to identify the candidate genes responsible for enhancing memory and evaluate the specific gene expression patterns by real-time PCR in SD rat model of passive avoidance task. ..

Labeling:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Hybridization:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Article Title: Ectonucleoside triphosphate diphosphohydrolase 6 expression in testis and testicular cancer and its implication in cisplatin resistance.
Article Snippet: After 48 h, the surviving cells were stained with the alamarBlue assay (treK Diagnostic systems, cleveland, oH, UsA) for 180 min at 37°c. the absorbances of the wells were measured using a plate reader (ArVoTM MX; perkin elmer Inc., Waltham, MA, UsA). cDNA microarray analysis. the procedure of cDnA microarray analysis was performed as described previously (18). .. Briefly, cDNA generation, hybridization, and data collection for cDnA microarray analyses were performed by the Incyte corporation (palo Alto, cA, UsA). ..

Clone Assay:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Amplification:

Article Title: Identification of Novel Peroxisome Proliferator-Activated Receptor α (PPARα) Target Genes in Mouse Liver Using cDNA Microarray Analysis
Article Snippet: Poly(A) + selected RNA was isolated using the Oligotex kit (Qiagen, Valencia, CA) from wild-type untreated mice, and wild-type mice treated with Wy-14,643 (0.125%, w/w) for 2 weeks. .. Poly(A) + RNA was labeled with Cy3 and Cy5 fluorescent dyes for microarray hybridization on UniGene mouse cDNA microarray (Incyte Genomics, St Louis, MO), as described elsewhere ( 77 ). cDNA mouse clones, corresponding to spotted cDNAs on the microarray, were obtained from Incyte Genomics (St. Louis, MO), amplified and sequenced to verify the correct identity of each clone. .. The data were analyzed using Incyte GEMTools vision 2.4.2a software (Incyte).

Over Expression:

Article Title: Differentially expressed genes in large granular lymphocyte leukemia
Article Snippet: .. Overexpression of a variety cytotoxic genes was observed in leukemic LGL utilizing cDNA microarray from Incyte Genomics (FIG. 1 and Table 4). .. To verify the identity of these overexpressed genes, clones containing cDNA fragments of the selected genes were obtained from Incyte Genomics and confirmed by sequencing.

other:

Article Title: Three microarray platforms: an analysis of their concordance in profiling gene expression
Article Snippet: Incyte cDNA arrays and Operon long oligonucleotide (70-mer) arrays were printed in house, and Affymetrix 25-mer HG U133A arrays were purchased from the manufacturer.

Article Title: Multifactorial regulation of E-cadherin expression: an integrative study
Article Snippet: We have previously described our processing and normalization of NCI-60 transcript expression data from pin-spotted cDNA arrays (Incyte, Inc., Palo Alto, CA) ( 27 , 28 ), Affymetrix Hu-6800 arrays (Affymetrix, Sunnyvale, CA) ( 29 ), Affymetrix HG-U95 arrays ( 30 ), and Affymetrix HG-U133 ( 30 ).

Biomarker Discovery:

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..

Tandem Mass Spectroscopy:

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..

Western Blot:

Article Title: A review of Omics research in acupuncture: the relevance and future prospects for understanding the nature of meridians and acupoints.
Article Snippet: .. Insulin-like growth factor-1 (IGF-1) was one of the most notable up-regulated J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 597 598 J. Jia et al. / Journal of Ethnopharm acology 140 (2012) 594– 603 Table 1 (Continued) Ref/Year Acupoints Treatment Subject groups Targeted disease/symptom Tissue sample Technique Verification/ validation Identified candidate gene Implication Ko/2002 ST36 (Zusanli) EA SD rats (n = 7/group): normal/neuropathic pain/EA treatment/ Neuropathic pain RNA from spinal nerves [pooled RNA samples] cDNA microarray (Incyte genomics) Dot-blot Up: opioid receptor, MAP kinase, zinc finger protein, and tyrosine phosphatase related genes Pain and analgesic Pan/2011 MS6 (scalp acu motor areas)/BL10(Tianzhu)/GB20 (Fengchi)/ LI4 (Hegu)/PC6 (Neiguan)/BL40 (Weizhong)/SP6 (Sanyinjiao)/S36 (Zusanli) EA 35 human: drug treatment/EA + drug treatment/normal Acute ischemic stroke Serum protein 2-DE + MS/MS Western blot Up: Gelsolin, C3, down: Serpin G1 Neuroprotection Kim/2010 HT8 (Sobu) Acupuncture SD rats (n = 34): pup+ mother/pup + mother +acupuncture/maternally separated(MS)/MS + acupuncture/ Maternal separation Protein sample from hypothalamic area 2-DE + MALDITOF/TOF MS N.A. ..



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CD8αα+RT1B/D+ cells express both genes associated with professional antigen processing/presenting and NK cytotoxicity. (A) Multi-color flow cytometry analyses show a CD8αα+RT1B/D+ population (purple gate on RT1B-FITC vs CD8α-APC plot, left panel) in rat peripheral blood mononuclear cells (PBMC); right panel shows purified CD8αα+RT1B/D+ population as shown by both SSC vs FSC and CD8α vs RT1B/D plot. (B) Representative flow cytometric plots show each isolated population from glomeruli infiltrating leukocytes as indicated on the top of each plot. MΦ, macrophages. (C) A representative clustering analysis based on DNA microarray shows similar global gene expression patterns among all CD8αα+RT1B/D+ subpopulations from different sources in both LEW and WKY rats; glomeruli-infiltrating macrophages (MΦ) and pan T cells are shown as controls. (D) Summary of expressions of gene clusters associated with NK cells, APCs and T cells. n=4. Inset, RT-PCR detection of Tyrobp and control house keeper gene Gapdh. (E) Summary of expressions of genes associated with Ag processing/presentation for professional APCs; expression levels in (D) and (E) are mean normalized intensities for each gene in DNA microarray. n=4. (F) A representative RT-PCR shows expression of Ag presenting related α chain of rat MHC class II RT1B (Rt1bα) and invariant chain (Clip) in various cell populations as indicated; TcR ε chain (Tcrε) and Gapdh were used as controls.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: CD8αα + MHC-II + Cell with Capacity to Terminate Autoimmune Inflammation Is A Novel Antigen-Presenting NK-Like Cell in Rats

doi: 10.4049/jimmunol.1601207

Figure Lengend Snippet: CD8αα+RT1B/D+ cells express both genes associated with professional antigen processing/presenting and NK cytotoxicity. (A) Multi-color flow cytometry analyses show a CD8αα+RT1B/D+ population (purple gate on RT1B-FITC vs CD8α-APC plot, left panel) in rat peripheral blood mononuclear cells (PBMC); right panel shows purified CD8αα+RT1B/D+ population as shown by both SSC vs FSC and CD8α vs RT1B/D plot. (B) Representative flow cytometric plots show each isolated population from glomeruli infiltrating leukocytes as indicated on the top of each plot. MΦ, macrophages. (C) A representative clustering analysis based on DNA microarray shows similar global gene expression patterns among all CD8αα+RT1B/D+ subpopulations from different sources in both LEW and WKY rats; glomeruli-infiltrating macrophages (MΦ) and pan T cells are shown as controls. (D) Summary of expressions of gene clusters associated with NK cells, APCs and T cells. n=4. Inset, RT-PCR detection of Tyrobp and control house keeper gene Gapdh. (E) Summary of expressions of genes associated with Ag processing/presentation for professional APCs; expression levels in (D) and (E) are mean normalized intensities for each gene in DNA microarray. n=4. (F) A representative RT-PCR shows expression of Ag presenting related α chain of rat MHC class II RT1B (Rt1bα) and invariant chain (Clip) in various cell populations as indicated; TcR ε chain (Tcrε) and Gapdh were used as controls.

Article Snippet: Rat DNA microarray assay Total RNA from various cell populations were isolated with a kit (Ambion, Austin, TX) and checked for their quality.

Techniques: Flow Cytometry, Purification, Isolation, Microarray, Gene Expression, Reverse Transcription Polymerase Chain Reaction, Control, Expressing

CD8αα+RT1B/D+ cells express NK killing stimulatory receptors Ly49s6. (A) DNA microarray shows expression of various NK receptors in PBMC (P) and glomeruli-infiltrating (G) CD8αα+RT1B/D+ cells, but not in control PBMC T cells and glomeruli-infiltrating macrophages; NK receptor genes are grouped; gene Klrb1a which encodes hallmark of an NK subset NKR-P1B is underlined; note that many Ly49 members were not covered by DNA microarray. (B) RT-PCR detection of mRNAs for representative Ly49 receptors in the indicated cell populations; lower panel shows degenerative PCR products for transmembrane (TM) and intracellular fragment of Ly49 in glomeruli-infiltrating CD8αα+RT1B/D+ cells. (C) Alignment of a.a. sequences of cloned cDNA from degenerative PCR (see lower panel in (B) as connected by lines) demonstrates expression of mainly killing stimulatory receptors in CD8αα+RT1B/D+ cells; numbers of clones for each sequence are indicated at the right with majority of them being Ly49s6; inhibitory motif in intracellular fragment of Ly49 is underlined in red at the bottom; TM, transmembrane. Lower panel shows a.a. sequence of cloned Ly49s6 with transmembrane domain shadowed. (D) Three-color flow cytometry on glomeruli-infiltrating leukocytes after removal of pan T cells shows a RT1B/D+Ly49s6+ population (lower panel) which is also CD8α+ (upper panel for the gated population in lower panel). (E) Immunofluorescence shows expression of both Ly49s6 (red) and RT1B (green) on a CD8αα+RT1B/D+ cell; a nearby T cell is shown as an internal control for staining. bar= 3μm

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: CD8αα + MHC-II + Cell with Capacity to Terminate Autoimmune Inflammation Is A Novel Antigen-Presenting NK-Like Cell in Rats

doi: 10.4049/jimmunol.1601207

Figure Lengend Snippet: CD8αα+RT1B/D+ cells express NK killing stimulatory receptors Ly49s6. (A) DNA microarray shows expression of various NK receptors in PBMC (P) and glomeruli-infiltrating (G) CD8αα+RT1B/D+ cells, but not in control PBMC T cells and glomeruli-infiltrating macrophages; NK receptor genes are grouped; gene Klrb1a which encodes hallmark of an NK subset NKR-P1B is underlined; note that many Ly49 members were not covered by DNA microarray. (B) RT-PCR detection of mRNAs for representative Ly49 receptors in the indicated cell populations; lower panel shows degenerative PCR products for transmembrane (TM) and intracellular fragment of Ly49 in glomeruli-infiltrating CD8αα+RT1B/D+ cells. (C) Alignment of a.a. sequences of cloned cDNA from degenerative PCR (see lower panel in (B) as connected by lines) demonstrates expression of mainly killing stimulatory receptors in CD8αα+RT1B/D+ cells; numbers of clones for each sequence are indicated at the right with majority of them being Ly49s6; inhibitory motif in intracellular fragment of Ly49 is underlined in red at the bottom; TM, transmembrane. Lower panel shows a.a. sequence of cloned Ly49s6 with transmembrane domain shadowed. (D) Three-color flow cytometry on glomeruli-infiltrating leukocytes after removal of pan T cells shows a RT1B/D+Ly49s6+ population (lower panel) which is also CD8α+ (upper panel for the gated population in lower panel). (E) Immunofluorescence shows expression of both Ly49s6 (red) and RT1B (green) on a CD8αα+RT1B/D+ cell; a nearby T cell is shown as an internal control for staining. bar= 3μm

Article Snippet: Rat DNA microarray assay Total RNA from various cell populations were isolated with a kit (Ambion, Austin, TX) and checked for their quality.

Techniques: Microarray, Expressing, Control, Reverse Transcription Polymerase Chain Reaction, Clone Assay, Sequencing, Flow Cytometry, Immunofluorescence, Staining

Activated autoreactive T cells with elevated level of RT1CE16 are more susceptible to the killing by CD8αα+RT1B/D+Ly49s6+ cells. (A) Summary of classic (RT1A) and non-classic (RT1C/E, M/N and S/T) MHC class I gene cluster expression pattern in PBMC (P) and glomeruli-infiltrating (GIL) T cells; expression level is normalized intensities in DNA microarray. n=4. (B) Flow cytometry shows detection of T cell activation marker CD69 in PBL pan T cells or glomeruli-infiltrating T cells as indicated. (C) Conventional RT-PCR detection of representative non-classic MHC I genes in PBL, splenic (SP), and glomeruli-infiltrating T cells. (D) Quantitative PCR detection of Rt1ce16 expression in glomeruli-infiltrating or PBL T cells. Relative expression levels to house-keeping gene Gapdh are shown. (E) Summary of susceptibility of T cells to CD8αα+RT1B/D+Ly49s6+ cell-mediated killing. Killing efficacy is expressed as % of remaining CFSE-labeled target T cells. T cells alone were used as control. (F) Correlation between RT1CE16 expression level in resting and activated pCol(28–40)-specific T cell lines and their susceptibilities to CD8αα+RT1B/D+Ly49s6+ cell-mediated killing; RT1CE16 expression levels were measured by qPCR and expressed as % of average for activated T cells; linear progression was calculated; each sample was duplicated. (G) Schematic diagram depicts three-step killing of autoreactive T cells by CD8αα+RT1B/D+Ly49s6+ cells (renamed to CD8αα+ AP-NK cell): Step 1, Coupling of CD8αα+ AP-NK cell with effector autoreactive T cell through its Ag presentation. Step 2, Binding of Ly49s/CD8αα on CD8αα+ AP-NK cell to RT1CE16 on the T cell. Step 3, DAP12 mediated release of granule cytotoxic proteins Gzm C and perforin from CD8αα+ AP-NK cell, which induce apoptosis in the target T cell.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: CD8αα + MHC-II + Cell with Capacity to Terminate Autoimmune Inflammation Is A Novel Antigen-Presenting NK-Like Cell in Rats

doi: 10.4049/jimmunol.1601207

Figure Lengend Snippet: Activated autoreactive T cells with elevated level of RT1CE16 are more susceptible to the killing by CD8αα+RT1B/D+Ly49s6+ cells. (A) Summary of classic (RT1A) and non-classic (RT1C/E, M/N and S/T) MHC class I gene cluster expression pattern in PBMC (P) and glomeruli-infiltrating (GIL) T cells; expression level is normalized intensities in DNA microarray. n=4. (B) Flow cytometry shows detection of T cell activation marker CD69 in PBL pan T cells or glomeruli-infiltrating T cells as indicated. (C) Conventional RT-PCR detection of representative non-classic MHC I genes in PBL, splenic (SP), and glomeruli-infiltrating T cells. (D) Quantitative PCR detection of Rt1ce16 expression in glomeruli-infiltrating or PBL T cells. Relative expression levels to house-keeping gene Gapdh are shown. (E) Summary of susceptibility of T cells to CD8αα+RT1B/D+Ly49s6+ cell-mediated killing. Killing efficacy is expressed as % of remaining CFSE-labeled target T cells. T cells alone were used as control. (F) Correlation between RT1CE16 expression level in resting and activated pCol(28–40)-specific T cell lines and their susceptibilities to CD8αα+RT1B/D+Ly49s6+ cell-mediated killing; RT1CE16 expression levels were measured by qPCR and expressed as % of average for activated T cells; linear progression was calculated; each sample was duplicated. (G) Schematic diagram depicts three-step killing of autoreactive T cells by CD8αα+RT1B/D+Ly49s6+ cells (renamed to CD8αα+ AP-NK cell): Step 1, Coupling of CD8αα+ AP-NK cell with effector autoreactive T cell through its Ag presentation. Step 2, Binding of Ly49s/CD8αα on CD8αα+ AP-NK cell to RT1CE16 on the T cell. Step 3, DAP12 mediated release of granule cytotoxic proteins Gzm C and perforin from CD8αα+ AP-NK cell, which induce apoptosis in the target T cell.

Article Snippet: Rat DNA microarray assay Total RNA from various cell populations were isolated with a kit (Ambion, Austin, TX) and checked for their quality.

Techniques: Expressing, Microarray, Flow Cytometry, Activation Assay, Marker, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Labeling, Control, Binding Assay