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neurospheres  (Carl Zeiss)


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    Structured Review

    Carl Zeiss neurospheres
    Neurospheres, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 993 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Inverted+Microscopes/Inverted+microscope+Axio+Observer+7/pm42869880-45-5-22
    Average 98 stars, based on 993 article reviews
    neurospheres - by Bioz Stars, 2026-10
    98/100 stars

    Images

    Related Articles

    other:

    Article Title: Ultrasound endometrial repair score correlates with histological repair and fertility outcomes in rat intrauterine adhesions.
    Article Snippet: Differentiation was evaluated under an inverted microscope (Axio Observer 7, Carl Zeiss Microscopy, Germany).

    Article Title: Loss of the fatty acid β-oxidation gene acaa-2 impairs timely hatching under poor maternal diet in C. elegans
    Article Snippet: As in , a cut piece of NGM agar was inverted onto a 25 × 36 mm coverslip (Matsunami, Cat. No. C025361) and mounted on an inverted microscope (Axio Observer 7, Zeiss) equipped with an ORCA-Fusion BT camera (Hamamatsu Photonics) and Colibri 7 LED light source (Zeiss).

    Fluorescence:

    Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system
    Article Snippet: .. Images were acquired using a ZEISS Axio Observer 7 inverted fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany) equipped with appropriate filter sets. ..

    Article Title: Engineering Spatially Localized Bioactive Microenvironments in Assembly Implants for Spatially Extended Bone Ingrowth
    Article Snippet: For CCK‐8 analysis, 300 μL of 10% CCK‐8 working solution (Dojindo, Japan) was added to each sample and incubated at 37°C for 2 h. Subsequently, 100 μL of the reaction solution was transferred to a 96‐well plate, and absorbance was measured at 450 nm. .. For live/dead staining, samples were incubated with 1 mL of Calcein‐AM/PI staining solution (Beyotime, China) at 37°C in the dark for 30 min, washed with PBS, and imaged using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany). ..

    Article Title: TiFM2.0 – versatile mechanical measurement and actuation in live embryos
    Article Snippet: Voltage controller , Sending driving programmes to the electrical piezo, voltages are related to X C although offsets and an error margin exist , Thorlabs , MDT693B , x. .. Base microscope , Inverted widefield and fluorescence. Axio Observer 7, 2.5/5/10/20× objectives, xy motorised stage, side camera port, fluorescence filters and LED light source for RL imaging, objective holder z -axis recording, associated controllers , Visualisation of the probes (X T ) and the sample. Different magnifications are essential for adjusting probe location followed by fine alignment. Z -axis recording is essential for determining probe insertion depth. Top illumination and condenser removed to allow installation of the probe head , Zeiss , https://www.micro-shop.zeiss.com/en/uk/system/inverted+microscopes-axio+observer-microscopes/6001/ , x (any inverted scope). ..

    Microscopy:

    Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system
    Article Snippet: .. Images were acquired using a ZEISS Axio Observer 7 inverted fluorescence microscope (Carl Zeiss AG, Oberkochen, Germany) equipped with appropriate filter sets. ..

    Article Title: Engineering Spatially Localized Bioactive Microenvironments in Assembly Implants for Spatially Extended Bone Ingrowth
    Article Snippet: For CCK‐8 analysis, 300 μL of 10% CCK‐8 working solution (Dojindo, Japan) was added to each sample and incubated at 37°C for 2 h. Subsequently, 100 μL of the reaction solution was transferred to a 96‐well plate, and absorbance was measured at 450 nm. .. For live/dead staining, samples were incubated with 1 mL of Calcein‐AM/PI staining solution (Beyotime, China) at 37°C in the dark for 30 min, washed with PBS, and imaged using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany). ..

    Article Title: MED12 mutation activates Notch signaling to enhance cancer stemness and suppress CD8+ T cell cytotoxicity in lung cancer
    Article Snippet: .. On day 14, the formed spheres were photographed under an inverted Zeiss Axio Observer 7 microscope (Zeiss, Germany), and the number of spheres (diameter > 50 μm) was counted in five random fields per well. ..

    Article Title: TiFM2.0 – versatile mechanical measurement and actuation in live embryos
    Article Snippet: Voltage controller , Sending driving programmes to the electrical piezo, voltages are related to X C although offsets and an error margin exist , Thorlabs , MDT693B , x. .. Base microscope , Inverted widefield and fluorescence. Axio Observer 7, 2.5/5/10/20× objectives, xy motorised stage, side camera port, fluorescence filters and LED light source for RL imaging, objective holder z -axis recording, associated controllers , Visualisation of the probes (X T ) and the sample. Different magnifications are essential for adjusting probe location followed by fine alignment. Z -axis recording is essential for determining probe insertion depth. Top illumination and condenser removed to allow installation of the probe head , Zeiss , https://www.micro-shop.zeiss.com/en/uk/system/inverted+microscopes-axio+observer-microscopes/6001/ , x (any inverted scope). ..

    Staining:

    Article Title: Engineering Spatially Localized Bioactive Microenvironments in Assembly Implants for Spatially Extended Bone Ingrowth
    Article Snippet: For CCK‐8 analysis, 300 μL of 10% CCK‐8 working solution (Dojindo, Japan) was added to each sample and incubated at 37°C for 2 h. Subsequently, 100 μL of the reaction solution was transferred to a 96‐well plate, and absorbance was measured at 450 nm. .. For live/dead staining, samples were incubated with 1 mL of Calcein‐AM/PI staining solution (Beyotime, China) at 37°C in the dark for 30 min, washed with PBS, and imaged using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany). ..

    Incubation:

    Article Title: Engineering Spatially Localized Bioactive Microenvironments in Assembly Implants for Spatially Extended Bone Ingrowth
    Article Snippet: For CCK‐8 analysis, 300 μL of 10% CCK‐8 working solution (Dojindo, Japan) was added to each sample and incubated at 37°C for 2 h. Subsequently, 100 μL of the reaction solution was transferred to a 96‐well plate, and absorbance was measured at 450 nm. .. For live/dead staining, samples were incubated with 1 mL of Calcein‐AM/PI staining solution (Beyotime, China) at 37°C in the dark for 30 min, washed with PBS, and imaged using an inverted fluorescence microscope (Axio Observer 7, Zeiss, Germany). ..

    Inverted Microscopy:

    Article Title: Stanniocalcin 1 contributes to glioma stem cells phenotypic plasticity through NOTCH1/STAT3/GFPT2 axis.
    Article Snippet: .. After 7 to 10 days, neurospheres with diameter over 50 μm were photographed and counted using an inverted microscope (Axio Observer 7, ZEISS, Germany), and then measured the diameter with Image J software (National Institutes of Health, USA). .. GSCs were pretreated with FBS-free DMEM/F12 and seeded onto 8-μm pole filters (BD Falcon, USA) and matrigel (Corning, USA)-coated filters, respectively.

    Article Title: Protocol to study murine ovarian elasticity and composition in situ by integrating quantitative micro-elastography with light microscopy
    Article Snippet: .. The equipment and settings for the presented protocol used were as follows: a. Zeiss Axio Observer.Z1/7 inverted microscope with LSM 980 confocal scanner. b. ..

    Software:

    Article Title: Stanniocalcin 1 contributes to glioma stem cells phenotypic plasticity through NOTCH1/STAT3/GFPT2 axis.
    Article Snippet: .. After 7 to 10 days, neurospheres with diameter over 50 μm were photographed and counted using an inverted microscope (Axio Observer 7, ZEISS, Germany), and then measured the diameter with Image J software (National Institutes of Health, USA). .. GSCs were pretreated with FBS-free DMEM/F12 and seeded onto 8-μm pole filters (BD Falcon, USA) and matrigel (Corning, USA)-coated filters, respectively.

    Imaging:

    Article Title: TiFM2.0 – versatile mechanical measurement and actuation in live embryos
    Article Snippet: Voltage controller , Sending driving programmes to the electrical piezo, voltages are related to X C although offsets and an error margin exist , Thorlabs , MDT693B , x. .. Base microscope , Inverted widefield and fluorescence. Axio Observer 7, 2.5/5/10/20× objectives, xy motorised stage, side camera port, fluorescence filters and LED light source for RL imaging, objective holder z -axis recording, associated controllers , Visualisation of the probes (X T ) and the sample. Different magnifications are essential for adjusting probe location followed by fine alignment. Z -axis recording is essential for determining probe insertion depth. Top illumination and condenser removed to allow installation of the probe head , Zeiss , https://www.micro-shop.zeiss.com/en/uk/system/inverted+microscopes-axio+observer-microscopes/6001/ , x (any inverted scope). ..



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