Review



access array tm ifc 4 primer amplicon tagging workflow  (fluidigm)


Bioz Verified Symbol fluidigm is a verified supplier
Bioz Manufacturer Symbol fluidigm manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    fluidigm access array tm ifc 4 primer amplicon tagging workflow
    Access Array Tm Ifc 4 Primer Amplicon Tagging Workflow, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Array+Tm+Ifc/Access+Array/pmc07865055-270-32-40
    Average 93 stars, based on 843 article reviews
    access array tm ifc 4 primer amplicon tagging workflow - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Paralleled Dynamics of Arabidopsis Root Exudation and SynCom Assembly in a Controlled Environment
    Article Snippet: PCR1 amplicons were purified using the ChargeSwitch® PCR Clean-Up Kit (Invitrogen, Waltham, MA, USA) on a KingFisherTM FlexTM Purification System (ThermoFisher Scientific, Waltham, MA, USA). .. Unique Access Array barcoding primers (Standard BioTools, CA, USA) were added in a second PCR (PCR2) using 5 μL of purified PCR1 product following previously reported cycling conditions (Rensburg et al., 2025). ..

    Article Title: Argan fruit microbiomes: influence of biogeographic and soil driven environmental factors
    Article Snippet: A second PCR was used to attach Illumina sequencing adapters and index tags. .. This reaction included 5 μL of purified PCR product, 2.5 μL of Fluidigm Access Array Barcode 384, and 1X KAPA HiFi HotStart ReadyMix (Roche Sequencing Solutions), in total volume of 50 μl. ..

    Purification:

    Article Title: Paralleled Dynamics of Arabidopsis Root Exudation and SynCom Assembly in a Controlled Environment
    Article Snippet: PCR1 amplicons were purified using the ChargeSwitch® PCR Clean-Up Kit (Invitrogen, Waltham, MA, USA) on a KingFisherTM FlexTM Purification System (ThermoFisher Scientific, Waltham, MA, USA). .. Unique Access Array barcoding primers (Standard BioTools, CA, USA) were added in a second PCR (PCR2) using 5 μL of purified PCR1 product following previously reported cycling conditions (Rensburg et al., 2025). ..

    Article Title: Argan fruit microbiomes: influence of biogeographic and soil driven environmental factors
    Article Snippet: A second PCR was used to attach Illumina sequencing adapters and index tags. .. This reaction included 5 μL of purified PCR product, 2.5 μL of Fluidigm Access Array Barcode 384, and 1X KAPA HiFi HotStart ReadyMix (Roche Sequencing Solutions), in total volume of 50 μl. ..

    High Throughput Screening Assay:

    Article Title: High-frequency oscillatory ventilation during physiological-based cord clamping attenuates inflammation in preterm lambs.
    Article Snippet: 10.1136/arch d isch ild -2025-329610 o n is C h ild F etal N eo n atal E d : first p u b lish ed as F3Benincasa BC, et al. Arch Dis Child Fetal Neonatal Ed 2026;0:F1–F8. doi:10.1136/archdischild- 2025- 329610 Original research and CD163- positive cells were manually quantified using Fiji/ ImageJ (V.2.14.0, LOCI, Wisconsin, USA) and expressed as total and relative to total cell counts. .. High- throughput real- time quantitative PCR using Fluidigm Access Array System Technology (Fluidigm, California, USA) and TaqMan primers (Thermo Fisher Scientific, Massachusetts, USA) was undertaken to quantify messenger RNA (mRNA) expression of 20 curated genes (online supplemental table 1). ..

    Real-time Polymerase Chain Reaction:

    Article Title: High-frequency oscillatory ventilation during physiological-based cord clamping attenuates inflammation in preterm lambs.
    Article Snippet: 10.1136/arch d isch ild -2025-329610 o n is C h ild F etal N eo n atal E d : first p u b lish ed as F3Benincasa BC, et al. Arch Dis Child Fetal Neonatal Ed 2026;0:F1–F8. doi:10.1136/archdischild- 2025- 329610 Original research and CD163- positive cells were manually quantified using Fiji/ ImageJ (V.2.14.0, LOCI, Wisconsin, USA) and expressed as total and relative to total cell counts. .. High- throughput real- time quantitative PCR using Fluidigm Access Array System Technology (Fluidigm, California, USA) and TaqMan primers (Thermo Fisher Scientific, Massachusetts, USA) was undertaken to quantify messenger RNA (mRNA) expression of 20 curated genes (online supplemental table 1). ..

    Expressing:

    Article Title: High-frequency oscillatory ventilation during physiological-based cord clamping attenuates inflammation in preterm lambs.
    Article Snippet: 10.1136/arch d isch ild -2025-329610 o n is C h ild F etal N eo n atal E d : first p u b lish ed as F3Benincasa BC, et al. Arch Dis Child Fetal Neonatal Ed 2026;0:F1–F8. doi:10.1136/archdischild- 2025- 329610 Original research and CD163- positive cells were manually quantified using Fiji/ ImageJ (V.2.14.0, LOCI, Wisconsin, USA) and expressed as total and relative to total cell counts. .. High- throughput real- time quantitative PCR using Fluidigm Access Array System Technology (Fluidigm, California, USA) and TaqMan primers (Thermo Fisher Scientific, Massachusetts, USA) was undertaken to quantify messenger RNA (mRNA) expression of 20 curated genes (online supplemental table 1). ..

    Amplification:

    Article Title: Fungal community structure and network connectivity as indicators of soil health under long-term land use.
    Article Snippet: Sequencing (Miseq) was performed at the Hylabs Laboratory Ltd. (Rehovot, Israel; www.hylabs.co.il) sequencing facility using an Illumina sequencing platform (Illumina Inc., San Diego, CA, USA). .. Protocol PCR2: 2ul sample from PCR1 amplified sample containing CS1/CS2 adaptors was amplified for 10 cycles in 10ul using Fluidigm Access Array Barcode library according to manufacturer’s protocol (2ul barcode per reaction). .. DNA was purified using Kapa Pure Beads at a ratio of 0.65X and quantified with qubit using Denovix DsDNA high sensitivity assay.

    Sequencing:

    Article Title: Genetic variations in AAK1 and ADAM17 associated with circulatory cytokines changes influence COVID-19 susceptibility and severity
    Article Snippet: .. The samples were prepared using the Fluidigm 48.48 Access Array integrated fluidic circuit (IFC) (Fluidigm Europe B.V., Netherlands) for sequencing using the Ion 520TM Chip on an Ion S5 XL Semiconductor (ThermoFisher, USA) as previously described [ ]. ..

    Article Title: Genetic variations in AAK1 and ADAM17 associated with circulatory cytokines changes influence COVID-19 susceptibility and severity.
    Article Snippet: .. The samples were prepared using the Fluidigm 48.48 Access Array integrated fluidic circuit (IFC) (Fluidigm Europe B.V., Netherlands) for sequencing using the Ion 520TM Chip on an Ion S5 XL Semiconductor (ThermoFisher, USA) as previously described (30). ..

    Mutagenesis:

    Article Title: Familial medullary thyroid carcinoma secondary to an SLC30A9 intragenic deletion and translation reinitiation
    Article Snippet: .. The probands from the RET mutation-negative MTC families and all patients with RET mutation-negative sporadic MTC were sequenced for SLC30A9 (NM_006345.4) using the Fluidigm Access Array (Fluidigm, South San Francisco, CA, USA). ..

    Immunopeptidomics:

    Article Title: Dynamics of natural and pharmacologic control of an SIV variant with an envelope trafficking defect
    Article Snippet: .. Briefly, a panel of oligonucleotides flanking the highly polymorphic peptide domains encoded by exon 2 of MHC class I and class II loci were used for multiplex PCRs with an Access Array 48.48 (Standard BioTools) following the manufacturer’s protocol. .. This amplicon library was sequenced on an Illumina MiSeq instrument, and the resulting sequence reads were mapped against a custom reference database of Mane MHC sequences ( https://github.com/dholab/mhc_genotyper ).

    Multiplex Assay:

    Article Title: Dynamics of natural and pharmacologic control of an SIV variant with an envelope trafficking defect
    Article Snippet: .. Briefly, a panel of oligonucleotides flanking the highly polymorphic peptide domains encoded by exon 2 of MHC class I and class II loci were used for multiplex PCRs with an Access Array 48.48 (Standard BioTools) following the manufacturer’s protocol. .. This amplicon library was sequenced on an Illumina MiSeq instrument, and the resulting sequence reads were mapped against a custom reference database of Mane MHC sequences ( https://github.com/dholab/mhc_genotyper ).



    Similar Products

    86
    Biomark Inc real time pcr dynamic array tm ifc platform
    Real Time Pcr Dynamic Array Tm Ifc Platform, supplied by Biomark Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Array+Tm+Ifc/48+48+array+biomark+dynamic/pmc12559939-3-8-7
    Average 86 stars, based on 1 article reviews
    real time pcr dynamic array tm ifc platform - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    93
    fluidigm array tm ifc
    Array Tm Ifc, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Array+Tm+Ifc/Assay+Loading+Reagent/pm39855563-97-8-14
    Average 93 stars, based on 1 article reviews
    array tm ifc - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    95
    fluidigm array tm integrated fluidic circuits
    Array Tm Integrated Fluidic Circuits, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Array+Tm+Ifc/96%2E96+Dynamic+Array+IFC+for+Genotyping/pmc11507445-179-3-8
    Average 95 stars, based on 1 article reviews
    array tm integrated fluidic circuits - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    fluidigm tm instrument
    Tm Instrument, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Array+Tm+Ifc/96%2E96+Dynamic+Array+IFC+for+Gene+Expression/pmc09861536-125-38-40
    Average 95 stars, based on 1 article reviews
    tm instrument - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    96
    fluidigm biomark tm ifc dynamic array tm genotyping
    Estimations of duck genome retention in the RH clones. A: retention frequencies of thirty-one microsatellite markers and four scaffold markers before (white) and after (grey) whole genome amplification. The test was done on the 90 selected hybrids by conventional Agarose <t>genotyping.</t> The expected chromosome locations of the markers (given in brackets) are derived from the chicken/duck comparative FISH mapping and a duck genetic map (Marie-Etancelin et al., in prep) for the microsatellite markers and according to comparative genomic data given by the Narcisse software for the scaffold markers. B : Retention frequencies of thirty-nine scaffolds markers obtained using three different genotyping strategies. The thirty-nine scaffold markers were genotyped using either (i) the amplified panel with conventional agarose genotyping (blue: WGA-PCR), (ii) the non amplified panel and genotyping with the Fluidigm BioMark gene expression dynamic array (green: Pre-ampFLDMqPCR) or (iii) the amplified panel and genotyping with the Fluidigm BioMark TM <t>IFC</t> Dynamic Array TM genotyping by quantitative PCR without any pre-amplification step (purple: WGA-FLDMqPCR). The markers are distributed along the X axis from the lowest to the highest retention frequencies obtained by the first method (the amplified panel with conventional agarose genotyping WGA-PCR in blue).
    Biomark Tm Ifc Dynamic Array Tm Genotyping, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Array+Tm+Ifc/Biomark/pmc03496577-172-9-8
    Average 96 stars, based on 1 article reviews
    biomark tm ifc dynamic array tm genotyping - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    90
    fluidigm 96.96 dynamic array tm integrated fluidic circuit (ifc)
    Estimations of duck genome retention in the RH clones. A: retention frequencies of thirty-one microsatellite markers and four scaffold markers before (white) and after (grey) whole genome amplification. The test was done on the 90 selected hybrids by conventional Agarose <t>genotyping.</t> The expected chromosome locations of the markers (given in brackets) are derived from the chicken/duck comparative FISH mapping and a duck genetic map (Marie-Etancelin et al., in prep) for the microsatellite markers and according to comparative genomic data given by the Narcisse software for the scaffold markers. B : Retention frequencies of thirty-nine scaffolds markers obtained using three different genotyping strategies. The thirty-nine scaffold markers were genotyped using either (i) the amplified panel with conventional agarose genotyping (blue: WGA-PCR), (ii) the non amplified panel and genotyping with the Fluidigm BioMark gene expression dynamic array (green: Pre-ampFLDMqPCR) or (iii) the amplified panel and genotyping with the Fluidigm BioMark TM <t>IFC</t> Dynamic Array TM genotyping by quantitative PCR without any pre-amplification step (purple: WGA-FLDMqPCR). The markers are distributed along the X axis from the lowest to the highest retention frequencies obtained by the first method (the amplified panel with conventional agarose genotyping WGA-PCR in blue).
    96.96 Dynamic Array Tm Integrated Fluidic Circuit (Ifc), supplied by fluidigm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Array+Tm+Ifc/48+48+dynamic+array+integrated+fluidic+circuits/pmc09604607-93-31-47
    Average 90 stars, based on 1 article reviews
    96.96 dynamic array tm integrated fluidic circuit (ifc) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    95
    fluidigm 48 48 dynamic array tm integrated fluid circuit
    Estimations of duck genome retention in the RH clones. A: retention frequencies of thirty-one microsatellite markers and four scaffold markers before (white) and after (grey) whole genome amplification. The test was done on the 90 selected hybrids by conventional Agarose <t>genotyping.</t> The expected chromosome locations of the markers (given in brackets) are derived from the chicken/duck comparative FISH mapping and a duck genetic map (Marie-Etancelin et al., in prep) for the microsatellite markers and according to comparative genomic data given by the Narcisse software for the scaffold markers. B : Retention frequencies of thirty-nine scaffolds markers obtained using three different genotyping strategies. The thirty-nine scaffold markers were genotyped using either (i) the amplified panel with conventional agarose genotyping (blue: WGA-PCR), (ii) the non amplified panel and genotyping with the Fluidigm BioMark gene expression dynamic array (green: Pre-ampFLDMqPCR) or (iii) the amplified panel and genotyping with the Fluidigm BioMark TM <t>IFC</t> Dynamic Array TM genotyping by quantitative PCR without any pre-amplification step (purple: WGA-FLDMqPCR). The markers are distributed along the X axis from the lowest to the highest retention frequencies obtained by the first method (the amplified panel with conventional agarose genotyping WGA-PCR in blue).
    48 48 Dynamic Array Tm Integrated Fluid Circuit, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Array+Tm+Ifc/48%2E48+Dynamic+Array+IFC+for+Genotyping/pmc08773042-78-11-19
    Average 95 stars, based on 1 article reviews
    48 48 dynamic array tm integrated fluid circuit - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    fluidigm array tm ifcs
    Estimations of duck genome retention in the RH clones. A: retention frequencies of thirty-one microsatellite markers and four scaffold markers before (white) and after (grey) whole genome amplification. The test was done on the 90 selected hybrids by conventional Agarose <t>genotyping.</t> The expected chromosome locations of the markers (given in brackets) are derived from the chicken/duck comparative FISH mapping and a duck genetic map (Marie-Etancelin et al., in prep) for the microsatellite markers and according to comparative genomic data given by the Narcisse software for the scaffold markers. B : Retention frequencies of thirty-nine scaffolds markers obtained using three different genotyping strategies. The thirty-nine scaffold markers were genotyped using either (i) the amplified panel with conventional agarose genotyping (blue: WGA-PCR), (ii) the non amplified panel and genotyping with the Fluidigm BioMark gene expression dynamic array (green: Pre-ampFLDMqPCR) or (iii) the amplified panel and genotyping with the Fluidigm BioMark TM <t>IFC</t> Dynamic Array TM genotyping by quantitative PCR without any pre-amplification step (purple: WGA-FLDMqPCR). The markers are distributed along the X axis from the lowest to the highest retention frequencies obtained by the first method (the amplified panel with conventional agarose genotyping WGA-PCR in blue).
    Array Tm Ifcs, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Array+Tm+Ifc/96%2E96+Dynamic+Array+IFC+for+Gene+Expression/bio_rxiv__2021__12__13__472353-116-24-27
    Average 95 stars, based on 1 article reviews
    array tm ifcs - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    90
    fluidigm 48.48 dynamic array tm ifcs
    Estimations of duck genome retention in the RH clones. A: retention frequencies of thirty-one microsatellite markers and four scaffold markers before (white) and after (grey) whole genome amplification. The test was done on the 90 selected hybrids by conventional Agarose <t>genotyping.</t> The expected chromosome locations of the markers (given in brackets) are derived from the chicken/duck comparative FISH mapping and a duck genetic map (Marie-Etancelin et al., in prep) for the microsatellite markers and according to comparative genomic data given by the Narcisse software for the scaffold markers. B : Retention frequencies of thirty-nine scaffolds markers obtained using three different genotyping strategies. The thirty-nine scaffold markers were genotyped using either (i) the amplified panel with conventional agarose genotyping (blue: WGA-PCR), (ii) the non amplified panel and genotyping with the Fluidigm BioMark gene expression dynamic array (green: Pre-ampFLDMqPCR) or (iii) the amplified panel and genotyping with the Fluidigm BioMark TM <t>IFC</t> Dynamic Array TM genotyping by quantitative PCR without any pre-amplification step (purple: WGA-FLDMqPCR). The markers are distributed along the X axis from the lowest to the highest retention frequencies obtained by the first method (the amplified panel with conventional agarose genotyping WGA-PCR in blue).
    48.48 Dynamic Array Tm Ifcs, supplied by fluidigm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/Array+Tm+Ifc/48+48+access+array+ifc/pmc08602333-454-19-34
    Average 90 stars, based on 1 article reviews
    48.48 dynamic array tm ifcs - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Estimations of duck genome retention in the RH clones. A: retention frequencies of thirty-one microsatellite markers and four scaffold markers before (white) and after (grey) whole genome amplification. The test was done on the 90 selected hybrids by conventional Agarose genotyping. The expected chromosome locations of the markers (given in brackets) are derived from the chicken/duck comparative FISH mapping and a duck genetic map (Marie-Etancelin et al., in prep) for the microsatellite markers and according to comparative genomic data given by the Narcisse software for the scaffold markers. B : Retention frequencies of thirty-nine scaffolds markers obtained using three different genotyping strategies. The thirty-nine scaffold markers were genotyped using either (i) the amplified panel with conventional agarose genotyping (blue: WGA-PCR), (ii) the non amplified panel and genotyping with the Fluidigm BioMark gene expression dynamic array (green: Pre-ampFLDMqPCR) or (iii) the amplified panel and genotyping with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping by quantitative PCR without any pre-amplification step (purple: WGA-FLDMqPCR). The markers are distributed along the X axis from the lowest to the highest retention frequencies obtained by the first method (the amplified panel with conventional agarose genotyping WGA-PCR in blue).

    Journal: BMC Genomics

    Article Title: A duck RH panel and its potential for assisting NGS genome assembly

    doi: 10.1186/1471-2164-13-513

    Figure Lengend Snippet: Estimations of duck genome retention in the RH clones. A: retention frequencies of thirty-one microsatellite markers and four scaffold markers before (white) and after (grey) whole genome amplification. The test was done on the 90 selected hybrids by conventional Agarose genotyping. The expected chromosome locations of the markers (given in brackets) are derived from the chicken/duck comparative FISH mapping and a duck genetic map (Marie-Etancelin et al., in prep) for the microsatellite markers and according to comparative genomic data given by the Narcisse software for the scaffold markers. B : Retention frequencies of thirty-nine scaffolds markers obtained using three different genotyping strategies. The thirty-nine scaffold markers were genotyped using either (i) the amplified panel with conventional agarose genotyping (blue: WGA-PCR), (ii) the non amplified panel and genotyping with the Fluidigm BioMark gene expression dynamic array (green: Pre-ampFLDMqPCR) or (iii) the amplified panel and genotyping with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping by quantitative PCR without any pre-amplification step (purple: WGA-FLDMqPCR). The markers are distributed along the X axis from the lowest to the highest retention frequencies obtained by the first method (the amplified panel with conventional agarose genotyping WGA-PCR in blue).

    Article Snippet: In our case, by performing qPCR with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping, the additional benefit is high throughput, as the identification of bands on gel electrophoresis is replaced by monitoring the PCR with Ct (Cycle threshold) and end point Tm (melting temperature) values, allowing the distinction between specific and non-specific amplification profiles.

    Techniques: Clone Assay, Whole Genome Amplification, Derivative Assay, Software, Amplification, Expressing, Real-time Polymerase Chain Reaction

    Genotyping by Fluidigm BioMarkTM IFC Dynamic ArrayTM quantitative PCR. (A) WGA-FLDMqPCR: WGA-amplified DNA and qPCR. Left: double-strand DNA (dsDNA) accumulation curve as a function of the number of cycles. Right: melting curve of the final product. Green: positive control (duck DNA). Red: a hybrid which was positive (containing duck DNA corresponding to the marker tested). Blue: a negative hybrid. Yellow: negative control (hamster DNA). (B) Pre-ampFLDMqPCR: non-amplified DNA, a pre-amplification step with a mix of the 96 primer pairs for the 96 markers tested in the Fluidigm BioMarkTM assay and qPCR. The same markers and controls are used as in (A) . The sensitivity is higher in (B) , with a lower number of cycles necessary for detection of duck DNA. The negative control and the hybrid not containing duck DNA amplify at a much higher number of cycles and the non-specific products amplified can easily be distinguished by their different melting temperature values (right). In both experiments, no amplification was obtained from water (data not shown).

    Journal: BMC Genomics

    Article Title: A duck RH panel and its potential for assisting NGS genome assembly

    doi: 10.1186/1471-2164-13-513

    Figure Lengend Snippet: Genotyping by Fluidigm BioMarkTM IFC Dynamic ArrayTM quantitative PCR. (A) WGA-FLDMqPCR: WGA-amplified DNA and qPCR. Left: double-strand DNA (dsDNA) accumulation curve as a function of the number of cycles. Right: melting curve of the final product. Green: positive control (duck DNA). Red: a hybrid which was positive (containing duck DNA corresponding to the marker tested). Blue: a negative hybrid. Yellow: negative control (hamster DNA). (B) Pre-ampFLDMqPCR: non-amplified DNA, a pre-amplification step with a mix of the 96 primer pairs for the 96 markers tested in the Fluidigm BioMarkTM assay and qPCR. The same markers and controls are used as in (A) . The sensitivity is higher in (B) , with a lower number of cycles necessary for detection of duck DNA. The negative control and the hybrid not containing duck DNA amplify at a much higher number of cycles and the non-specific products amplified can easily be distinguished by their different melting temperature values (right). In both experiments, no amplification was obtained from water (data not shown).

    Article Snippet: In our case, by performing qPCR with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping, the additional benefit is high throughput, as the identification of bands on gel electrophoresis is replaced by monitoring the PCR with Ct (Cycle threshold) and end point Tm (melting temperature) values, allowing the distinction between specific and non-specific amplification profiles.

    Techniques: Real-time Polymerase Chain Reaction, Amplification, Positive Control, Marker, Negative Control

     Genotyping  8 no hit markers using three different genotyping strategies

    Journal: BMC Genomics

    Article Title: A duck RH panel and its potential for assisting NGS genome assembly

    doi: 10.1186/1471-2164-13-513

    Figure Lengend Snippet: Genotyping 8 no hit markers using three different genotyping strategies

    Article Snippet: In our case, by performing qPCR with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping, the additional benefit is high throughput, as the identification of bands on gel electrophoresis is replaced by monitoring the PCR with Ct (Cycle threshold) and end point Tm (melting temperature) values, allowing the distinction between specific and non-specific amplification profiles.

    Techniques:

    Comparison of marker retention with the three  genotyping  techniques

    Journal: BMC Genomics

    Article Title: A duck RH panel and its potential for assisting NGS genome assembly

    doi: 10.1186/1471-2164-13-513

    Figure Lengend Snippet: Comparison of marker retention with the three genotyping techniques

    Article Snippet: In our case, by performing qPCR with the Fluidigm BioMark TM IFC Dynamic Array TM genotyping, the additional benefit is high throughput, as the identification of bands on gel electrophoresis is replaced by monitoring the PCR with Ct (Cycle threshold) and end point Tm (melting temperature) values, allowing the distinction between specific and non-specific amplification profiles.

    Techniques: Marker