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7000 sequence detection system instrument software  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 7000 sequence detection system instrument software
    7000 Sequence Detection System Instrument Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/7000+software+sequence+detection+software/pmc03478226-94-14-13
    Average 90 stars, based on 1 article reviews
    7000 sequence detection system instrument software - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: Protein Susceptibility to Peroxidation by 4-Hydroxynonenal in Hereditary Hemochromatosis
    Article Snippet: The tissue was previously stored at −80 °C in sections of approximately 2–5 mm in 300 μL of RNAlater solution (Catalog: #AM7020, Thermo Fisher Scientific, Waltham, MA, USA). mRNA was extracted in duplicate using the GeneJET RNA purification kit (Catalog: #K0702, Thermo Fisher Scientific, Waltham, MA, USA) including DNAse I (AM1906 Thermo Fisher Scientific, Waltham, MA, USA) digestion according to the manufacturer’s instructions. .. Subsequently, the cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Catalog: #4368814, Applied Biosystems, Waltham, MA, USA) for Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) in an ABI 7000 Sequence Detection System (Applied Biosystems, Waltham, MA, USA). .. PCR reactions were performed for commercial mixtures of primers and specific probes corresponding to the sequences of the hamp , tnf , il6 , sod2 , gpx and β- actin gene, which was used as constitutive expression (Catalog: #4331182, Applied Biosystems assays ).

    Reverse Transcription:

    Article Title: Protein Susceptibility to Peroxidation by 4-Hydroxynonenal in Hereditary Hemochromatosis
    Article Snippet: The tissue was previously stored at −80 °C in sections of approximately 2–5 mm in 300 μL of RNAlater solution (Catalog: #AM7020, Thermo Fisher Scientific, Waltham, MA, USA). mRNA was extracted in duplicate using the GeneJET RNA purification kit (Catalog: #K0702, Thermo Fisher Scientific, Waltham, MA, USA) including DNAse I (AM1906 Thermo Fisher Scientific, Waltham, MA, USA) digestion according to the manufacturer’s instructions. .. Subsequently, the cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Catalog: #4368814, Applied Biosystems, Waltham, MA, USA) for Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) in an ABI 7000 Sequence Detection System (Applied Biosystems, Waltham, MA, USA). .. PCR reactions were performed for commercial mixtures of primers and specific probes corresponding to the sequences of the hamp , tnf , il6 , sod2 , gpx and β- actin gene, which was used as constitutive expression (Catalog: #4331182, Applied Biosystems assays ).

    Article Title: Serum microRNA-205 as a novel biomarker for cervical cancer patients
    Article Snippet: For qPCR, miRNA-specific TaqMan MicroRNA Assays (Applied Biosystems, Foster City, CA, USA) for miR-16 (reference miR for serum), U6 (reference miR for tissue) and miR-205 were performed, as described by the manufacturer. .. Briefly, 100 ng of total RNA was reverse transcribed using primers specific to each miRNA target, followed by real-time PCR using an Applied Biosystems 7000 Sequence Detection System. ..

    Polymerase Chain Reaction:

    Article Title: Protein Susceptibility to Peroxidation by 4-Hydroxynonenal in Hereditary Hemochromatosis
    Article Snippet: The tissue was previously stored at −80 °C in sections of approximately 2–5 mm in 300 μL of RNAlater solution (Catalog: #AM7020, Thermo Fisher Scientific, Waltham, MA, USA). mRNA was extracted in duplicate using the GeneJET RNA purification kit (Catalog: #K0702, Thermo Fisher Scientific, Waltham, MA, USA) including DNAse I (AM1906 Thermo Fisher Scientific, Waltham, MA, USA) digestion according to the manufacturer’s instructions. .. Subsequently, the cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Catalog: #4368814, Applied Biosystems, Waltham, MA, USA) for Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) in an ABI 7000 Sequence Detection System (Applied Biosystems, Waltham, MA, USA). .. PCR reactions were performed for commercial mixtures of primers and specific probes corresponding to the sequences of the hamp , tnf , il6 , sod2 , gpx and β- actin gene, which was used as constitutive expression (Catalog: #4331182, Applied Biosystems assays ).

    Sequencing:

    Article Title: Protein Susceptibility to Peroxidation by 4-Hydroxynonenal in Hereditary Hemochromatosis
    Article Snippet: The tissue was previously stored at −80 °C in sections of approximately 2–5 mm in 300 μL of RNAlater solution (Catalog: #AM7020, Thermo Fisher Scientific, Waltham, MA, USA). mRNA was extracted in duplicate using the GeneJET RNA purification kit (Catalog: #K0702, Thermo Fisher Scientific, Waltham, MA, USA) including DNAse I (AM1906 Thermo Fisher Scientific, Waltham, MA, USA) digestion according to the manufacturer’s instructions. .. Subsequently, the cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Catalog: #4368814, Applied Biosystems, Waltham, MA, USA) for Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) in an ABI 7000 Sequence Detection System (Applied Biosystems, Waltham, MA, USA). .. PCR reactions were performed for commercial mixtures of primers and specific probes corresponding to the sequences of the hamp , tnf , il6 , sod2 , gpx and β- actin gene, which was used as constitutive expression (Catalog: #4331182, Applied Biosystems assays ).

    Article Title: The oncogenic fusion protein RUNX1-CBFA2T1 supports proliferation and inhibits senescence in t(8;21)-positive leukaemic cells
    Article Snippet: Reverse transcription was performed with random hexamers using MMLV-RT, RNase H - (Promega, Heidelberg, Germany) as suggested by the manufacturer. .. Real time PCRs were performed using Sybr-Green Mix (Applied Biosystems, Warrington, UK), 300 nM primers and 20% v/v diluted RT reaction mix using standard conditions on a 7000 Sequence detection system (Applied Biosystems, Foster City, CA, USA). .. The primer sequences for STAT1 (5'-CAT CAC ATT CAC ATG GGT GGA (forward primer) and 5'-GGT TCA ACC GCA TGG AAG TC (reverse primer)), for CD34 (5'-TCC AGA AAC GGC CAT TCA G (forward primer) and 5'-CCC ACC TAG CCG AGT CAC AA (reverse primer)), for G-CSF (5'-CCC ACC TTG GAC ACA CTG C (forward primer) and 5'-AGT TCT TCC ATC TGC TGC CAG (reverse primer)) and for GAPDH (5'-GAA GGT GAA GGT CGG AGT C (forward primer) and 5'-GAA GAT GGT GAT GGG ATT TC (reverse primer)) were designed with Primer-Express software (Applied Biosystems, Foster City, CA, USA).

    Article Title: Serum microRNA-205 as a novel biomarker for cervical cancer patients
    Article Snippet: For qPCR, miRNA-specific TaqMan MicroRNA Assays (Applied Biosystems, Foster City, CA, USA) for miR-16 (reference miR for serum), U6 (reference miR for tissue) and miR-205 were performed, as described by the manufacturer. .. Briefly, 100 ng of total RNA was reverse transcribed using primers specific to each miRNA target, followed by real-time PCR using an Applied Biosystems 7000 Sequence Detection System. ..

    Article Title: Bariatric Surgery as Treatment Strategy of Obesity in Saudi People: Effects of Gut Microbiota
    Article Snippet: .. RT-qPCR was performed using an ABI 7000 Sequence Detection System with software version 1.2.3 (Applied Biosystems, Foster City, CA, USA). .. Amplification and detection were carried out in 96-well plates with SYBR Green PCR 2× Master Mix (Applied Biosystems).

    Article Title: Angiopoietin-Like 4 Mediates PPAR Delta Effect on Lipoprotein Lipase-Dependent Fatty Acid Uptake but Not on Beta-Oxidation in Myotubes
    Article Snippet: The total RNA (2 μg) was reverse-transcribed using Maxima First Strand cDNA Synthesis Kit (Fermentas). .. Samples were amplified on a 7000 Sequence Detection System (Applied Biosystems), using FastStart Universal SYBR Green Mastermix with ROX (Roche). ..

    other:

    Article Title: Synergistic Use of Geniposide and Ginsenoside Rg1 Balance Microglial TNF- α and TGF- β 1 following Oxygen-Glucose Deprivation In Vitro : A Genome-Wide Survey
    Article Snippet: Five nanograms of total RNA was reverse-transcribed using oligo d(T) and Primer Script RT Enzyme MixI (TaKaRa) according to the manufacturer's instructions.

    Article Title: Transfer factors peptides (Imuno TF ® ) modulate the lung inflammation and airway remodeling in allergic asthma
    Article Snippet: PCR was performed in a 7000-sequence detection system (ABI Prism; Applied Biosystems, Foster City, CA, USA) using the SYBRGreen core reaction kit (Applied Biosystems).

    Article Title: Bone Marrow and Nonbone Marrow Toll Like Receptor 4 Regulate Acute Hepatic Injury Induced by Endotoxemia
    Article Snippet: cDNA was synthesized from total RNA using the TaqMan High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s protocol.

    Real-time Polymerase Chain Reaction:

    Article Title: Serum microRNA-205 as a novel biomarker for cervical cancer patients
    Article Snippet: For qPCR, miRNA-specific TaqMan MicroRNA Assays (Applied Biosystems, Foster City, CA, USA) for miR-16 (reference miR for serum), U6 (reference miR for tissue) and miR-205 were performed, as described by the manufacturer. .. Briefly, 100 ng of total RNA was reverse transcribed using primers specific to each miRNA target, followed by real-time PCR using an Applied Biosystems 7000 Sequence Detection System. ..

    Quantitative RT-PCR:

    Article Title: Bariatric Surgery as Treatment Strategy of Obesity in Saudi People: Effects of Gut Microbiota
    Article Snippet: .. RT-qPCR was performed using an ABI 7000 Sequence Detection System with software version 1.2.3 (Applied Biosystems, Foster City, CA, USA). .. Amplification and detection were carried out in 96-well plates with SYBR Green PCR 2× Master Mix (Applied Biosystems).

    Software:

    Article Title: Bariatric Surgery as Treatment Strategy of Obesity in Saudi People: Effects of Gut Microbiota
    Article Snippet: .. RT-qPCR was performed using an ABI 7000 Sequence Detection System with software version 1.2.3 (Applied Biosystems, Foster City, CA, USA). .. Amplification and detection were carried out in 96-well plates with SYBR Green PCR 2× Master Mix (Applied Biosystems).

    Amplification:

    Article Title: Angiopoietin-Like 4 Mediates PPAR Delta Effect on Lipoprotein Lipase-Dependent Fatty Acid Uptake but Not on Beta-Oxidation in Myotubes
    Article Snippet: The total RNA (2 μg) was reverse-transcribed using Maxima First Strand cDNA Synthesis Kit (Fermentas). .. Samples were amplified on a 7000 Sequence Detection System (Applied Biosystems), using FastStart Universal SYBR Green Mastermix with ROX (Roche). ..

    SYBR Green Assay:

    Article Title: Angiopoietin-Like 4 Mediates PPAR Delta Effect on Lipoprotein Lipase-Dependent Fatty Acid Uptake but Not on Beta-Oxidation in Myotubes
    Article Snippet: The total RNA (2 μg) was reverse-transcribed using Maxima First Strand cDNA Synthesis Kit (Fermentas). .. Samples were amplified on a 7000 Sequence Detection System (Applied Biosystems), using FastStart Universal SYBR Green Mastermix with ROX (Roche). ..



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