m-mlv rt 5x reaction buffer (Promega)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Promega m-mlv rt 5x reaction buffer
M Mlv Rt 5x Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+rt-buffer/mgcl2/pm40659902-129-3-26
Average 90 stars, based on 1 article reviews

m-mlv rt 5x reaction buffer - by Bioz Stars, 2026-10

90/100 stars

Images

Real-time Polymerase Chain Reaction:

Article Title: Oral exposure to LaNiO 3 regulates the immune system, modulates gut flora, and induces intestinal autophagy in mice
Article Snippet: About 10 pmol oligonucleotide (Oligo dT) (Sigma, USA) primer and Moloney murine leukemia virus reverse transcriptase (M-MLV, Promega, Madison, USA) were added to 2 mg of RNA to generate cDNA. .. Each sample was prepared for real-time quantitative PCR in a final reaction volume of 20 mL by adding Master Mix (Promega, Madison, USA) and SYBR Green (Invitrogen, Paisley, UK). .. The amplification cycle was performed using a Realplex4 (Eppendorf, Germany).

SYBR Green Assay:

other:

Article Title: Polyglutamylation of microtubules drives neuronal remodeling.
Article Snippet: 5μl of M-MLV Reverse Transcriptase Buffer (Promega #M531A), 10mM NTPs mixture, and 200μ of MMLV (Promega #M170A) were added, and the mixture was incubated for 1.5 h at 37 °C. cDNA was purified with a QIAEX II Gel Extraction Kit (Qiagen #20021) as follows: The obtained cDNAwasmixedwith 2μl QiaEX-II suspension (Silica-Matrix) and 80μl QX-I buffer, incubated for 20min at 25 °C at 1000 rpmand centrifuged at 13,000 x g for 2min.

Article Title: Eomes directs the formation of spatially and functionally diverse extraembryonic hematovascular tissues.
Article Snippet: After centrifuging again, the supernatant was removed and the cell pellet resuspended in 100 μl of ice-cold nuclear extraction (NE) buffer (10 mM Tris pH 7.5, 10 mM NaCl, 3 mM MgCl2, 1% BSA, 0.1% Tween, 1 mM DTT, 1 U/μl RNaseIn (Promega), 0.1% NP40, 0.01% Digitonin) and incubated on ice for 4 min. 1mL of wash buffer (identical to NE buffer but lacking NP40 and digitonin) was added and nuclei were centrifuged at 500 xg for 5 min at 4 ◦ C. Nuclei washed twice by resuspension in 1 mL of wash buffer and centifugation at 500 xg for 5 min at 4 ◦ C. Nuclei were resuspended in 50 μl of diluted nuclei buffer (10x Genomics) and 1 μl was used to assess quality using a microscope and count nuclei using a Countess II instrument.

Article Title: Cell wall modifications in Saccharomyces cerevisiae wine yeast through adaptive laboratory evolution with Tebuconazole.
Article Snippet: Ten μL de M-MLV RT 5X Reaction Buffer, 2.5 μL dNTPs (10 mM dATP, 10 mM dGTP, 10 mM dCTP y 10 mM dTTP) were added (Promega, USA), 1,2 μL Recombinant RNasin® Ribonuclease Inhibitor (40 U/μL), 2 μL of enzyme M-MLV RT (200 U/μL) y 10.3 μL of Nuclease free water was added, with a final volume of 50 μL.

Article Title: Tracking Plasmodium falciparum antimalarial resistance markers during a malaria pre-elimination period in the Pacific coast of South America
Article Snippet: Polymerase Chain Reaction (PCR) solution contained 12.5 μl of Colorless GoTaq Master Mix (Promega, Madison, WI, USA), 2.5 μl of MgCl 2 (25 mM) (Promega, Madison, WI, USA), 3 μl of ultrapure H 2 O, 1 μl of primers forward and reverse (10 μM) and 5 μl of DNA.

Article Title: Polyglutamylation of microtubules drives neuronal remodeling
Article Snippet: 5 μl of M-MLV Reverse Transcriptase Buffer (Promega #M531A), 10 mM NTPs mixture, and 200 μ of MMLV (Promega #M170A) were added, and the mixture was incubated for 1.5 h at 37 °C. cDNA was purified with a QIAEX II Gel Extraction Kit (Qiagen #20021) as follows: The obtained cDNA was mixed with 2 μl QiaEX-II suspension (Silica-Matrix) and 80 μl QX-I buffer, incubated for 20 min at 25 °C at 1000 rpm and centrifuged at 13,000 x g for 2 min.

Polymerase Chain Reaction:

Article Title: Novel protocol for mapping virus integration sites in genes involved in therapy resistance.
Article Snippet: .. The PCR was performed on a Biorad T100 thermal cycler using a 50 μL PCR reaction mix containing 1X GoTaq Flexi Buffer (Promega, cat.no M890A), 2 mM MgCl2 (Promega, cat.no A351H), 0.2 mM dNTPs (NEB, cat. No N0447S), 1.0 μM neomycinspecific primer (NeoP), 1.0 μM locus-specific primer (LSP), 1.25 U GoTaq G2 Flexi DNA polymerase (Promega, cat.no M780B), 10% Glycerol (Sigma-Aldrich, cat. No. G5516), UltraPureTM Distilled Water (Invitrogen, cat. No 10977035) and 10 ng gDNA. ..

Centrifugation:

Article Title: Decreasing the intrinsically disordered protein α-synuclein levels by targeting its structured mRNA with a ribonuclease targeting chimera
Article Snippet: .. After centrifugation at 5,000 rpm for 5 min, the supernatant was removed, and the cell pellets were lysed with 250 μL of ice-cold Polysome Extraction Buffer [10 mM Tris, pH 7.5, 10 mM NaCl, 10 mM MgCl2, 1% (v/v) Triton X-100, 1% (w/v) sodium deoxycholate, 1 mM dithiothreitol (DTT) supplemented with 100 μg/mL CHX and 0.2 U/μL RNasin (Promega)]. ..

Review

m-mlv rt 5x reaction buffer (Promega)

Structured Review

Images

Related Articles

Real-time Polymerase Chain Reaction:

SYBR Green Assay:

other:

Polymerase Chain Reaction:

Centrifugation:

Extraction:

Similar Products

Image Search Results