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pcr buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcr buffer
    Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5x+native+sample+loading+buffer/SM+buffer/pmc07519651-227-24-31
    Average 99 stars, based on 1 article reviews
    pcr buffer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: The intrinsically disordered protein SPE-56 is required for acrosomal-like exocytosis and fertility in Caenorhabditis elegans
    Article Snippet: .. In summary, isolated spermatids or in vitro-activated spermatozoa were incubated in sperm medium (SM buffer) containing 5 μg/mL FM 1–43 dye (obtained from Thermo Scientific) for a period of three minutes at room temperature. .. Subsequent to the process of incubation, the samples were mounted under a 20 × 20 mm glass coverslip and imaged immediately via a Zeiss laser scanning confocal microscope (LSM700) equipped with a 100-fold oil immersion objective and an AxioCam MRm camera (Zeiss).

    Article Title: The intrinsically disordered protein SPE-56 is required for acrosomal-like exocytosis and fertility in Caenorhabditis elegans
    Article Snippet: .. The activation status of the sperm was then assessed by incubating isolated sperm in sperm medium (SM buffer) supplemented with 5 μg/mL FM1-43 (Thermo Scientific) , for a period of three minutes. ..

    Article Title: The intrinsically disordered protein SPE-56 is required for acrosomal-like exocytosis and fertility in Caenorhabditis elegans.
    Article Snippet: .. L4-stage worms were transferred to 35 mm NGM agar plates and incubated at 20 °C for 48 h. For sperm isolation, adult worms were transferred to a 4.5 μL Scientific Reports | (2026) 16:12062 14| https://doi.org/10.1038/s41598-026-47896-7 droplet of sperm medium (SM buffer; 50 mM HEPES, 45 mM NaCl, 1 mM MgSO4, 25 mM KCl, 5 mM CaCl2, 10 mM dextrose; pH 7.8)65 placed on a glass microscope slide (Thermo Scientific) pre-marked with a hydrophobic PAP pen (DAKO). .. Coverslips (20 × 20 mm; Carl Roth) were then applied, and the samples were sealed with Vaseline.

    Article Title: The intrinsically disordered protein SPE-56 is required for acrosomal-like exocytosis and fertility in Caenorhabditis elegans.
    Article Snippet: .. In summary, isolated spermatids or in vitro-activated spermatozoa were incubated in sperm medium (SM buffer) containing 5 μg/mL FM 1–43 dye (obtained from Thermo Scientific) for a period of three minutes at room temperature. .. Subsequent to the process of incubation, the samples were mounted under a 20 × 20 mm glass coverslip and imaged immediately via a Zeiss laser scanning confocal microscope (LSM700) equipped with a 100-fold oil immersion objective and an AxioCam MRm camera (Zeiss).

    Incubation:

    Article Title: The intrinsically disordered protein SPE-56 is required for acrosomal-like exocytosis and fertility in Caenorhabditis elegans
    Article Snippet: .. In summary, isolated spermatids or in vitro-activated spermatozoa were incubated in sperm medium (SM buffer) containing 5 μg/mL FM 1–43 dye (obtained from Thermo Scientific) for a period of three minutes at room temperature. .. Subsequent to the process of incubation, the samples were mounted under a 20 × 20 mm glass coverslip and imaged immediately via a Zeiss laser scanning confocal microscope (LSM700) equipped with a 100-fold oil immersion objective and an AxioCam MRm camera (Zeiss).

    Article Title: The intrinsically disordered protein SPE-56 is required for acrosomal-like exocytosis and fertility in Caenorhabditis elegans.
    Article Snippet: .. L4-stage worms were transferred to 35 mm NGM agar plates and incubated at 20 °C for 48 h. For sperm isolation, adult worms were transferred to a 4.5 μL Scientific Reports | (2026) 16:12062 14| https://doi.org/10.1038/s41598-026-47896-7 droplet of sperm medium (SM buffer; 50 mM HEPES, 45 mM NaCl, 1 mM MgSO4, 25 mM KCl, 5 mM CaCl2, 10 mM dextrose; pH 7.8)65 placed on a glass microscope slide (Thermo Scientific) pre-marked with a hydrophobic PAP pen (DAKO). .. Coverslips (20 × 20 mm; Carl Roth) were then applied, and the samples were sealed with Vaseline.

    Article Title: The intrinsically disordered protein SPE-56 is required for acrosomal-like exocytosis and fertility in Caenorhabditis elegans.
    Article Snippet: .. In summary, isolated spermatids or in vitro-activated spermatozoa were incubated in sperm medium (SM buffer) containing 5 μg/mL FM 1–43 dye (obtained from Thermo Scientific) for a period of three minutes at room temperature. .. Subsequent to the process of incubation, the samples were mounted under a 20 × 20 mm glass coverslip and imaged immediately via a Zeiss laser scanning confocal microscope (LSM700) equipped with a 100-fold oil immersion objective and an AxioCam MRm camera (Zeiss).

    Article Title: Bacteriophage-Based Control of Methicillin-Resistant Staphylococcus aureus : Anti-Biofilm Activity, Surface-Active Formulation Compatibility, and Genomic Context.
    Article Snippet: Aliquots (1 mL) were spread onto Nutrient Agar (Himedia, Mumbai, India) supplemented with 0.1% (w/v) CaCl2 (Sigma-Aldrich, Burlington, MA, USA). .. After drying, 10 μL drops of filtered phage lysates were applied to the agar surface and incubated at 37 ◦C for 18–24 h. Plaque formation was recorded, and single plaques were excised and suspended in SM buffer (Thermo Fisher Scientific, Waltham, MA, USA) [28,29]. ..

    Article Title: Bacteriophage-Based Control of Methicillin-Resistant Staphylococcus aureus : Anti-Biofilm Activity, Surface-Active Formulation Compatibility, and Genomic Context.
    Article Snippet: Briefly, host bacteria were prepared as described above and overlaid onto Nutrient Agar (Himedia, Mumbai, India) containing 0.1% (w/v) CaCl2 (Sigma-Aldrich, MA, USA). .. Phage suspensions (10 μL per drop) were applied, incubated at 37 ◦C for 24 h, and individual plaques were recovered into SM buffer (Thermo Fisher Scientific, MA, USA). ..

    Activation Assay:

    Article Title: The intrinsically disordered protein SPE-56 is required for acrosomal-like exocytosis and fertility in Caenorhabditis elegans
    Article Snippet: .. The activation status of the sperm was then assessed by incubating isolated sperm in sperm medium (SM buffer) supplemented with 5 μg/mL FM1-43 (Thermo Scientific) , for a period of three minutes. ..

    Microscopy:

    Article Title: The intrinsically disordered protein SPE-56 is required for acrosomal-like exocytosis and fertility in Caenorhabditis elegans.
    Article Snippet: .. L4-stage worms were transferred to 35 mm NGM agar plates and incubated at 20 °C for 48 h. For sperm isolation, adult worms were transferred to a 4.5 μL Scientific Reports | (2026) 16:12062 14| https://doi.org/10.1038/s41598-026-47896-7 droplet of sperm medium (SM buffer; 50 mM HEPES, 45 mM NaCl, 1 mM MgSO4, 25 mM KCl, 5 mM CaCl2, 10 mM dextrose; pH 7.8)65 placed on a glass microscope slide (Thermo Scientific) pre-marked with a hydrophobic PAP pen (DAKO). .. Coverslips (20 × 20 mm; Carl Roth) were then applied, and the samples were sealed with Vaseline.

    other:

    Article Title: The intrinsically disordered protein SPE-56 is required for acrosomal-like exocytosis and fertility in Caenorhabditis elegans.
    Article Snippet: Subsequent to the process of incubation, the samples were mounted under a 20 × 20 mm glass coverslip and imaged immediately via a Zeiss laser scanning confocal microscope (LSM700) equipped with a 100-fold oil immersion objective and an AxioCam MRm camera (Zeiss).



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