Review




Structured Review

Promega random primers
Random Primers, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+goscripttm+buffer/random+primers/pm39623375-124-10-12
Average 90 stars, based on 1 article reviews
random primers - by Bioz Stars, 2026-11
90/100 stars

Images

Related Articles

other:

Article Title: Fluorescence damping as primary interference mechanism of humic acids on qPCR quantification of SARS-CoV-2 in wastewater surveillance.
Article Snippet: Real-time polymerase chain reaction (qPCR) is a widely used technology for the detection and quantification of nucleic acids in wastewater surveillance.. However, the presence of inhibiting and/or interfering substances in wastewater samples, in particular humic acids, can significantly impact the accuracy and reliability of qPCR results.. In this study, we investigated the impact of humic acids on qPCR reactions and determined the relative importance of various inhibition/interference mechanisms through spiked experiments and modeling.

Article Title: Reciprocal rescue of Wolfram syndrome by two causative genes.
Article Snippet: 1 μl of random primer (C1181, Promega) was added to 10 μl of RNA solution and incubated at 70 °C for 10min, after which the solution was cooled down.

Article Title: An in vitro model of the epithelial airway reveals a key function for EHF in lung homeostasis and disease
Article Snippet: Cell viability was determined by incubating cells with 1:10 Presto Blue reagent (Invitrogen) in Pneumacult ALI medium at 37°C for 4 h. The EnVision plate reader (excitation/emission of 560 nm/590 nm) was used to measure the corresponding fluorescence.

Article Title: MYC modulates TOP2A diffusion to promote substrate detection and activity
Article Snippet: Equal amounts of RNA resuspended in 13 ul H 2 O were mixed with 1 ul of 10 mM dNTP (10 mM each of dATP, dCTP, dGTP, dTTP; Promega, U1205, U1215, U1225, U1235) and 0.5 ul of random hexamers (Promega, C1181).

Generated:

Article Title: Supporting Information for EP300 facilitates human trophoblast stem cell differentiation
Article Snippet: Total RNA was extracted from monolayer cells and organoids using the RNeasy Mini Kit (QIAGEN, 74104) according to the manufacturer’s protocol. .. First-strand cDNA was generated from 1 μg total RNA using 500 ng Random Primers (Promega, C1181), 200 units M-MLV Reverse Transcriptase (Promega, M170B), 10 mM dNTP mix (Thermo Scientific, R0192) and 25 units Recombinant RNasin Ribonuclease Inhibitor (Promega, N251B) in a 25- μl reaction. .. The qPCR was prepared using 2.5 μl 3x diluted cDNA, 0.5 μM primers (see Table S7) and 1x LightCycler 480 SYBR Green I Master (Roche Diagnostics, 04887352001) in a total volume of 10 μl, and run on a LightCycler 480 system (Roche Diagnostics).

Reverse Transcription:

Article Title: Supporting Information for EP300 facilitates human trophoblast stem cell differentiation
Article Snippet: Total RNA was extracted from monolayer cells and organoids using the RNeasy Mini Kit (QIAGEN, 74104) according to the manufacturer’s protocol. .. First-strand cDNA was generated from 1 μg total RNA using 500 ng Random Primers (Promega, C1181), 200 units M-MLV Reverse Transcriptase (Promega, M170B), 10 mM dNTP mix (Thermo Scientific, R0192) and 25 units Recombinant RNasin Ribonuclease Inhibitor (Promega, N251B) in a 25- μl reaction. .. The qPCR was prepared using 2.5 μl 3x diluted cDNA, 0.5 μM primers (see Table S7) and 1x LightCycler 480 SYBR Green I Master (Roche Diagnostics, 04887352001) in a total volume of 10 μl, and run on a LightCycler 480 system (Roche Diagnostics).

Article Title: Inflammatory factors released by ACL reconstruction tendon grafts and their potential for macrophage polarization.
Article Snippet: RNA isolation was performed using the Qiagen RNeasy kit (Qiagen Benelux BV, Venlo, the Netherlands) according to manufacturer’s protocol. .. For cDNA synthesis, 250 ng RNA was synthesized in a 20 μl reaction volume containing Moloney Murine Leukemia Virus reverse transcriptase (M-MLV RT, 200 U/μL, Invitrogen, Fisher Scientific), dNTPs (10 mM, Invitrogen), random primers (50 ng/μl, Promega Benelux BV, Leiden, the Netherlands), 5x first strand buffer, and RNA-free water in a thermocycler (C1000 Touch, Bio-Rad Laboratories, Lunteren, the Netherlands) using the following protocol: 65 °C for 5 min, 37 °C for 2 min, 25 °C for 10 min, 37 °C for 50 min, and 70 °C for 15 min. Primer sequences used in this study are listed in Supplementary Table S3. .. These primers were used in combination with SYBR Green Supermix (Bio-Rad) and RNA-free water for qPCR analysis using a CFX Opus 384 real-time PCR system (Bio-Rad). cDNA was diluted ± 30 times and analyzed in duplicates.

Article Title: YTHDC1 promotes postnatal brown adipose tissue development and thermogenesis by stabilizing PPARγ.
Article Snippet: Total RNA was extracted using TriPure Isolation Reagent (94015120, Roche). .. First-strand cDNA synthesis was performed using Random Primers and M-MLV reverse transcriptase (M1701, Promega) (Li et al, 2018; Ren et al, 2017). .. Gene expression was measured using SYBR Green Mix (4913914001, Roche) and analyzed using a Roche LightCycler 480 real-time PCR system (Roche, Mannheim, Germany).

Article Title: Rice stripe virus NS3 uses the host signaling pathways to control pathogenicity.
Article Snippet: Article Rice stripe virus NS3 uses the host signaling pathways to control pathogenicity

Recombinant:

Article Title: Supporting Information for EP300 facilitates human trophoblast stem cell differentiation
Article Snippet: Total RNA was extracted from monolayer cells and organoids using the RNeasy Mini Kit (QIAGEN, 74104) according to the manufacturer’s protocol. .. First-strand cDNA was generated from 1 μg total RNA using 500 ng Random Primers (Promega, C1181), 200 units M-MLV Reverse Transcriptase (Promega, M170B), 10 mM dNTP mix (Thermo Scientific, R0192) and 25 units Recombinant RNasin Ribonuclease Inhibitor (Promega, N251B) in a 25- μl reaction. .. The qPCR was prepared using 2.5 μl 3x diluted cDNA, 0.5 μM primers (see Table S7) and 1x LightCycler 480 SYBR Green I Master (Roche Diagnostics, 04887352001) in a total volume of 10 μl, and run on a LightCycler 480 system (Roche Diagnostics).

cDNA Synthesis:

Article Title: Inflammatory factors released by ACL reconstruction tendon grafts and their potential for macrophage polarization.
Article Snippet: RNA isolation was performed using the Qiagen RNeasy kit (Qiagen Benelux BV, Venlo, the Netherlands) according to manufacturer’s protocol. .. For cDNA synthesis, 250 ng RNA was synthesized in a 20 μl reaction volume containing Moloney Murine Leukemia Virus reverse transcriptase (M-MLV RT, 200 U/μL, Invitrogen, Fisher Scientific), dNTPs (10 mM, Invitrogen), random primers (50 ng/μl, Promega Benelux BV, Leiden, the Netherlands), 5x first strand buffer, and RNA-free water in a thermocycler (C1000 Touch, Bio-Rad Laboratories, Lunteren, the Netherlands) using the following protocol: 65 °C for 5 min, 37 °C for 2 min, 25 °C for 10 min, 37 °C for 50 min, and 70 °C for 15 min. Primer sequences used in this study are listed in Supplementary Table S3. .. These primers were used in combination with SYBR Green Supermix (Bio-Rad) and RNA-free water for qPCR analysis using a CFX Opus 384 real-time PCR system (Bio-Rad). cDNA was diluted ± 30 times and analyzed in duplicates.

Article Title: YTHDC1 promotes postnatal brown adipose tissue development and thermogenesis by stabilizing PPARγ.
Article Snippet: Total RNA was extracted using TriPure Isolation Reagent (94015120, Roche). .. First-strand cDNA synthesis was performed using Random Primers and M-MLV reverse transcriptase (M1701, Promega) (Li et al, 2018; Ren et al, 2017). .. Gene expression was measured using SYBR Green Mix (4913914001, Roche) and analyzed using a Roche LightCycler 480 real-time PCR system (Roche, Mannheim, Germany).

Synthesized:

Article Title: Inflammatory factors released by ACL reconstruction tendon grafts and their potential for macrophage polarization.
Article Snippet: RNA isolation was performed using the Qiagen RNeasy kit (Qiagen Benelux BV, Venlo, the Netherlands) according to manufacturer’s protocol. .. For cDNA synthesis, 250 ng RNA was synthesized in a 20 μl reaction volume containing Moloney Murine Leukemia Virus reverse transcriptase (M-MLV RT, 200 U/μL, Invitrogen, Fisher Scientific), dNTPs (10 mM, Invitrogen), random primers (50 ng/μl, Promega Benelux BV, Leiden, the Netherlands), 5x first strand buffer, and RNA-free water in a thermocycler (C1000 Touch, Bio-Rad Laboratories, Lunteren, the Netherlands) using the following protocol: 65 °C for 5 min, 37 °C for 2 min, 25 °C for 10 min, 37 °C for 50 min, and 70 °C for 15 min. Primer sequences used in this study are listed in Supplementary Table S3. .. These primers were used in combination with SYBR Green Supermix (Bio-Rad) and RNA-free water for qPCR analysis using a CFX Opus 384 real-time PCR system (Bio-Rad). cDNA was diluted ± 30 times and analyzed in duplicates.

Virus:

Article Title: Inflammatory factors released by ACL reconstruction tendon grafts and their potential for macrophage polarization.
Article Snippet: RNA isolation was performed using the Qiagen RNeasy kit (Qiagen Benelux BV, Venlo, the Netherlands) according to manufacturer’s protocol. .. For cDNA synthesis, 250 ng RNA was synthesized in a 20 μl reaction volume containing Moloney Murine Leukemia Virus reverse transcriptase (M-MLV RT, 200 U/μL, Invitrogen, Fisher Scientific), dNTPs (10 mM, Invitrogen), random primers (50 ng/μl, Promega Benelux BV, Leiden, the Netherlands), 5x first strand buffer, and RNA-free water in a thermocycler (C1000 Touch, Bio-Rad Laboratories, Lunteren, the Netherlands) using the following protocol: 65 °C for 5 min, 37 °C for 2 min, 25 °C for 10 min, 37 °C for 50 min, and 70 °C for 15 min. Primer sequences used in this study are listed in Supplementary Table S3. .. These primers were used in combination with SYBR Green Supermix (Bio-Rad) and RNA-free water for qPCR analysis using a CFX Opus 384 real-time PCR system (Bio-Rad). cDNA was diluted ± 30 times and analyzed in duplicates.



Similar Products

90
Promega goscripttm 5x rt reaction buffer
Goscripttm 5x Rt Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+goscripttm+buffer/goscript+5x+reaction+buffer/pmc11680345-65-10-15
Average 90 stars, based on 1 article reviews
goscripttm 5x rt reaction buffer - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
Promega goscripttm 5x reaction buffer
Goscripttm 5x Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+goscripttm+buffer/goscript+5x+reaction+buffer/pmc11533326-188-20-24
Average 90 stars, based on 1 article reviews
goscripttm 5x reaction buffer - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
Promega 5x goscripttm buffer
5x Goscripttm Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+goscripttm+buffer/goscript+5x+reaction+buffer/pm37169322-93-10-24
Average 90 stars, based on 1 article reviews
5x goscripttm buffer - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
Promega goscripttm (promega®, madison, wi) 5x reaction buffer
Goscripttm (Promega®, Madison, Wi) 5x Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+goscripttm+buffer/goscript+5x+reaction+buffer/10__1097_slash_md__0000000000031588-75-35-36
Average 90 stars, based on 1 article reviews
goscripttm (promega®, madison, wi) 5x reaction buffer - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

90
Promega goscripttm 5x buffer
Goscripttm 5x Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+goscripttm+buffer/goscript+5x+reaction+buffer/pm29879485-84-54-57
Average 90 stars, based on 1 article reviews
goscripttm 5x buffer - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

Image Search Results