goscripttm 5x rt reaction buffer (Promega)
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Promega
goscripttm 5x rt reaction buffer
Goscripttm 5x Rt Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+goscripttm+buffer/goscript+5x+reaction+buffer/pmc11680345-65-10-15
Average 90 stars, based on 1 article reviews
Goscripttm 5x Rt Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+goscripttm+buffer/goscript+5x+reaction+buffer/pmc11680345-65-10-15
Average 90 stars, based on 1 article reviews
goscripttm 5x rt reaction buffer - by Bioz Stars,
2026-09
90/100 stars
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Concentration Assay:Article Title: Dimethyl Sulfoxide (DMSO) as a Potential Source of Interference in Research Related to Sulfur Metabolism—A Preliminary Study Article Snippet: For the reaction, 2 μg of the total RNA were combined with water pretreated with diethylpyrocarbonate (DEPC-H 2 O, Thermo Fisher Scientific, Waltham, MA, USA) and with 1 μL of Oligo(dT) 15 primer (0.5 μg/reaction, Thermo Fisher Scientific, Waltham, MA, USA) and incubated for 5 min at 70 °C. .. Then, a mixture with the following components was added: 4 μL of the Reverse Transcription:Article Title: Dimethyl Sulfoxide (DMSO) as a Potential Source of Interference in Research Related to Sulfur Metabolism—A Preliminary Study Article Snippet: For the reaction, 2 μg of the total RNA were combined with water pretreated with diethylpyrocarbonate (DEPC-H 2 O, Thermo Fisher Scientific, Waltham, MA, USA) and with 1 μL of Oligo(dT) 15 primer (0.5 μg/reaction, Thermo Fisher Scientific, Waltham, MA, USA) and incubated for 5 min at 70 °C. .. Then, a mixture with the following components was added: 4 μL of the Article Title: Establishment and Application of Mismatch Amplification Mutation Assay-PCR for Rapid Detection and Differentiation of Duck Hepatitis A Virus-1 Attenuated Vaccine and Wild Strains Article Snippet: .. Subsequently, a mixture was prepared to contain 4 μL of Article Title: Targeting SOD1 via RNAi with PEGylated graphene oxide nanoparticles in platinum-resistant ovarian cancer. Article Snippet: Total RNA (500 ng) was annealed with Oligo(dT15) primers at 70 °C for 5 min, then cooled immediately in ice water for 5 min and centrifuged for 10 s. SOD1 mRNA level was quantified using the (forward) 5′-ATCCTCTATCCAGAAAACACGG and 5′-GCGTTTCCTGTCTTTGTACTTT (reverse), while β-actin housekeeping gene with 5′-CACCATTGGCAATGAGCGGTTCC-3′ (forward) and 5′-GTAGTTTCGTGGATGCCACAGG-3′ (reverse) primer sequences (Sangon Biotech, Shanghai, China) was used as an internal reference. .. The samples were mixed with 4.0 μL Article Title: The Impact of Genetic Variation on Duck Hepatitis A Virus (DHAV) Vaccine Efficacy: A Comparative Study of DHAV-1 and DHAV-3 Against Emerging Variant Strains Article Snippet: In the RT steps, 1 μL of random primer (9-mers; TaKaRa Bio Inc., Kusatsu, Japan) was added to every 10 μL of extracted RNA, heated at 70 °C for 5 min, followed by cooling in an ice bath for 5 min. .. Subsequently, a mixture was prepared to contain 4 μL of Article Title: Targeting SOD1 via RNAi with PEGylated graphene oxide nanoparticles in platinum-resistant ovarian cancer Article Snippet: Total RNA (500 ng) was annealed with Oligo(dT15) primers at 70 °C for 5 min, then cooled immediately in ice water for 5 min and centrifuged for 10 s. SOD1 mRNA level was quantified using the (forward) 5′-ATCCTCTATCCAGAAAACACGG and 5′-GCGTTTCCTGTCTTTGTACTTT (reverse), while β-actin housekeeping gene with 5′-CACCATTGGCAATGAGCGGTTCC-3′ (forward) and 5′-GTAGTTTCGTGGATGCCACAGG-3′ (reverse) primer sequences (Sangon Biotech, Shanghai, China) was used as an internal reference. .. The samples were mixed with 4.0 μL Article Title: Nutritional status affects Igf1 regulation of skeletal muscle myogenesis, myostatin, and myofibrillar protein degradation pathways in gopher rockfish (Sebastes carnatus). Article Snippet: Total RNA was DNase I treated (TURBO DNA-free Kit, Ambion) and quantified by spectrophotometry (P300 NanoPhotometer, Implen; 260:280 ratios ≥1.96). .. Total RNA was then reverse transcribed using 5.6 μl of other:Article Title: Simultaneous construction strategy using two types of fluorescent markers for HVT vector vaccine against infectious bursal disease and H9N2 avian influenza virus by NHEJ-CRISPR/Cas9 Article Snippet: In the reverse transcription (RT) reaction, 8 μL of extracted RNA and 2 μL of dimethyl sulfoxide (Tedia, Ohio, USA) were heated at 100°C for 5 min and then placed in an ice bath for 5 min. Then, the following materials were added to this reaction mixture: 8 μL of Recombinant:Article Title: Establishment and Application of Mismatch Amplification Mutation Assay-PCR for Rapid Detection and Differentiation of Duck Hepatitis A Virus-1 Attenuated Vaccine and Wild Strains Article Snippet: .. Subsequently, a mixture was prepared to contain 4 μL of Article Title: The Impact of Genetic Variation on Duck Hepatitis A Virus (DHAV) Vaccine Efficacy: A Comparative Study of DHAV-1 and DHAV-3 Against Emerging Variant Strains Article Snippet: In the RT steps, 1 μL of random primer (9-mers; TaKaRa Bio Inc., Kusatsu, Japan) was added to every 10 μL of extracted RNA, heated at 70 °C for 5 min, followed by cooling in an ice bath for 5 min. .. Subsequently, a mixture was prepared to contain 4 μL of Article Title: Nutritional status affects Igf1 regulation of skeletal muscle myogenesis, myostatin, and myofibrillar protein degradation pathways in gopher rockfish (Sebastes carnatus). Article Snippet: Total RNA was DNase I treated (TURBO DNA-free Kit, Ambion) and quantified by spectrophotometry (P300 NanoPhotometer, Implen; 260:280 ratios ≥1.96). .. Total RNA was then reverse transcribed using 5.6 μl of Polymerase Chain Reaction:Article Title: Targeting SOD1 via RNAi with PEGylated graphene oxide nanoparticles in platinum-resistant ovarian cancer. Article Snippet: Total RNA (500 ng) was annealed with Oligo(dT15) primers at 70 °C for 5 min, then cooled immediately in ice water for 5 min and centrifuged for 10 s. SOD1 mRNA level was quantified using the (forward) 5′-ATCCTCTATCCAGAAAACACGG and 5′-GCGTTTCCTGTCTTTGTACTTT (reverse), while β-actin housekeeping gene with 5′-CACCATTGGCAATGAGCGGTTCC-3′ (forward) and 5′-GTAGTTTCGTGGATGCCACAGG-3′ (reverse) primer sequences (Sangon Biotech, Shanghai, China) was used as an internal reference. .. The samples were mixed with 4.0 μL Article Title: Targeting SOD1 via RNAi with PEGylated graphene oxide nanoparticles in platinum-resistant ovarian cancer Article Snippet: Total RNA (500 ng) was annealed with Oligo(dT15) primers at 70 °C for 5 min, then cooled immediately in ice water for 5 min and centrifuged for 10 s. SOD1 mRNA level was quantified using the (forward) 5′-ATCCTCTATCCAGAAAACACGG and 5′-GCGTTTCCTGTCTTTGTACTTT (reverse), while β-actin housekeeping gene with 5′-CACCATTGGCAATGAGCGGTTCC-3′ (forward) and 5′-GTAGTTTCGTGGATGCCACAGG-3′ (reverse) primer sequences (Sangon Biotech, Shanghai, China) was used as an internal reference. .. The samples were mixed with 4.0 μL Article Title: Improvement of succinate production from methane by combining rational engineering and laboratory evolution in Methylomonas sp. DH-1 Article Snippet: Extracted total RNA was converted to cDNA by reverse transcription, performed in a total 20 μL reactions with GoScriptTM reverse transcriptase (Promega, USA). .. 10 pmol of Random primer (Biorad, USA), 10 mM PCR nucleotide Mix (Promega, USA), 25 mM MgCl 2 (Promega, USA), Random Hexamer:Article Title: Nutritional status affects Igf1 regulation of skeletal muscle myogenesis, myostatin, and myofibrillar protein degradation pathways in gopher rockfish (Sebastes carnatus). Article Snippet: Total RNA was DNase I treated (TURBO DNA-free Kit, Ambion) and quantified by spectrophotometry (P300 NanoPhotometer, Implen; 260:280 ratios ≥1.96). .. Total RNA was then reverse transcribed using 5.6 μl of Real-time Polymerase Chain Reaction:Article Title: Nutritional status affects Igf1 regulation of skeletal muscle myogenesis, myostatin, and myofibrillar protein degradation pathways in gopher rockfish (Sebastes carnatus). Article Snippet: Total RNA was DNase I treated (TURBO DNA-free Kit, Ambion) and quantified by spectrophotometry (P300 NanoPhotometer, Implen; 260:280 ratios ≥1.96). .. Total RNA was then reverse transcribed using 5.6 μl of |