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recombinant rnasin ® ribonuclease inhibitor  (Promega)

 
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    Structured Review

    Promega recombinant rnasin ® ribonuclease inhibitor
    Recombinant Rnasin ® Ribonuclease Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5x+goscripttm+buffer/rnase+inhibitor/pmc11428521-62-40-45
    Average 90 stars, based on 1 article reviews
    recombinant rnasin ® ribonuclease inhibitor - by Bioz Stars, 2026-09
    90/100 stars

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    Purification:

    Article Title: Delivery and formulation of engineered nucleic acids
    Article Snippet: The resulting capped and polyadenylated synthetic mRNA is then purified by denaturing agarose gel electrophoresis to confirm production of full-length product and to remove any degradation products followed by spin column filtration (RNeasy Kit, Qiagen, Valencia, CA; MEGACLEARTM AMBION®, Austin, TX). .. Purified synthetic mRNAs are resuspended in RNase-free water containing an RNase inhibitor (RNASIN® Plus RNase Inhibitor, Promega, Madison, WI), quantified by NANODROPTM (Thermo Scientific, Logan, UT) and stored at −20° C. .. A. Cationic Lipid Delivery Vehicles RNA transfections are carried out using RNAIMax (Invitrogen, Carlsbad, CA) or TRANSIT-mRNA (Mirus Bio, Madison, WI) cationic lipid delivery vehicles.

    Generated:

    Article Title: Supporting Information for EP300 facilitates human trophoblast stem cell differentiation
    Article Snippet: Total RNA was extracted from monolayer cells and organoids using the RNeasy Mini Kit (QIAGEN, 74104) according to the manufacturer’s protocol. .. First-strand cDNA was generated from 1 μg total RNA using 500 ng Random Primers (Promega, C1181), 200 units M-MLV Reverse Transcriptase (Promega, M170B), 10 mM dNTP mix (Thermo Scientific, R0192) and 25 units Recombinant RNasin Ribonuclease Inhibitor (Promega, N251B) in a 25- μl reaction. .. The qPCR was prepared using 2.5 μl 3x diluted cDNA, 0.5 μM primers (see Table S7) and 1x LightCycler 480 SYBR Green I Master (Roche Diagnostics, 04887352001) in a total volume of 10 μl, and run on a LightCycler 480 system (Roche Diagnostics).

    Reverse Transcription:

    Article Title: Supporting Information for EP300 facilitates human trophoblast stem cell differentiation
    Article Snippet: Total RNA was extracted from monolayer cells and organoids using the RNeasy Mini Kit (QIAGEN, 74104) according to the manufacturer’s protocol. .. First-strand cDNA was generated from 1 μg total RNA using 500 ng Random Primers (Promega, C1181), 200 units M-MLV Reverse Transcriptase (Promega, M170B), 10 mM dNTP mix (Thermo Scientific, R0192) and 25 units Recombinant RNasin Ribonuclease Inhibitor (Promega, N251B) in a 25- μl reaction. .. The qPCR was prepared using 2.5 μl 3x diluted cDNA, 0.5 μM primers (see Table S7) and 1x LightCycler 480 SYBR Green I Master (Roche Diagnostics, 04887352001) in a total volume of 10 μl, and run on a LightCycler 480 system (Roche Diagnostics).

    Article Title: Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ
    Article Snippet: .. At this point, 1 μl of this sample was set-aside for quality control. ssDNA amplification from template RNA was performed by reverse transcription, using: 150 μl of unpurified in vitro transcription reaction, 1.7 mM dNTP mix, 19 μM forward primer, 240 U RNAsin Plus RNAse inhibitor (Promega), 1200 U Maxima H Minus Reverse Transcriptase (ThermoFisher), 60 μl of 5x Maxima Buffer and water up to 300 μl. ..

    Recombinant:

    Article Title: Supporting Information for EP300 facilitates human trophoblast stem cell differentiation
    Article Snippet: Total RNA was extracted from monolayer cells and organoids using the RNeasy Mini Kit (QIAGEN, 74104) according to the manufacturer’s protocol. .. First-strand cDNA was generated from 1 μg total RNA using 500 ng Random Primers (Promega, C1181), 200 units M-MLV Reverse Transcriptase (Promega, M170B), 10 mM dNTP mix (Thermo Scientific, R0192) and 25 units Recombinant RNasin Ribonuclease Inhibitor (Promega, N251B) in a 25- μl reaction. .. The qPCR was prepared using 2.5 μl 3x diluted cDNA, 0.5 μM primers (see Table S7) and 1x LightCycler 480 SYBR Green I Master (Roche Diagnostics, 04887352001) in a total volume of 10 μl, and run on a LightCycler 480 system (Roche Diagnostics).

    Incubation:

    Article Title:
    Article Snippet: .. Fragmented RNA was incubated for 2 hr at 4C with 0.5 mg/ml antim6A polyclonal antibody in IP buffer (150 mM NaCl, 0.1% Igepal CA-630, 10 mM Tris-HCl [pH 7.4]) supplemented with RNasin Plus RNase inhibitor (Promega). ..

    Control:

    Article Title: Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ
    Article Snippet: .. At this point, 1 μl of this sample was set-aside for quality control. ssDNA amplification from template RNA was performed by reverse transcription, using: 150 μl of unpurified in vitro transcription reaction, 1.7 mM dNTP mix, 19 μM forward primer, 240 U RNAsin Plus RNAse inhibitor (Promega), 1200 U Maxima H Minus Reverse Transcriptase (ThermoFisher), 60 μl of 5x Maxima Buffer and water up to 300 μl. ..

    Amplification:

    Article Title: Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ
    Article Snippet: .. At this point, 1 μl of this sample was set-aside for quality control. ssDNA amplification from template RNA was performed by reverse transcription, using: 150 μl of unpurified in vitro transcription reaction, 1.7 mM dNTP mix, 19 μM forward primer, 240 U RNAsin Plus RNAse inhibitor (Promega), 1200 U Maxima H Minus Reverse Transcriptase (ThermoFisher), 60 μl of 5x Maxima Buffer and water up to 300 μl. ..

    In Vitro:

    Article Title: Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ
    Article Snippet: .. At this point, 1 μl of this sample was set-aside for quality control. ssDNA amplification from template RNA was performed by reverse transcription, using: 150 μl of unpurified in vitro transcription reaction, 1.7 mM dNTP mix, 19 μM forward primer, 240 U RNAsin Plus RNAse inhibitor (Promega), 1200 U Maxima H Minus Reverse Transcriptase (ThermoFisher), 60 μl of 5x Maxima Buffer and water up to 300 μl. ..

    Lysis:

    Article Title: Supporting Information for Pairwise biosynthesis of ion channels stabilizes excitability and mitigates arrhythmias
    Article Snippet: .. It was then transferred to a tube with lysis buffer containing T-Per Tissue Protein Extraction Reagent (Promega), 1:100 Protease Inhibitor Cocktail (Biotool Chemicals LLC), 1:500 RNAsin Ribonuclease Inhibitor (Promega). ..

    Protein Extraction:

    Article Title: Supporting Information for Pairwise biosynthesis of ion channels stabilizes excitability and mitigates arrhythmias
    Article Snippet: .. It was then transferred to a tube with lysis buffer containing T-Per Tissue Protein Extraction Reagent (Promega), 1:100 Protease Inhibitor Cocktail (Biotool Chemicals LLC), 1:500 RNAsin Ribonuclease Inhibitor (Promega). ..

    Protease Inhibitor:

    Article Title: Supporting Information for Pairwise biosynthesis of ion channels stabilizes excitability and mitigates arrhythmias
    Article Snippet: .. It was then transferred to a tube with lysis buffer containing T-Per Tissue Protein Extraction Reagent (Promega), 1:100 Protease Inhibitor Cocktail (Biotool Chemicals LLC), 1:500 RNAsin Ribonuclease Inhibitor (Promega). ..



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