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Promega pcr nucleotide mix
Pcr Nucleotide Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+goscripttm+buffer/pcr+nucleotide+mix/pm32781964-107-21-46
Average 90 stars, based on 1 article reviews
pcr nucleotide mix - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

One Step RT-PCR:

Article Title: Phylogenetic Study of Local Patterns Influenza A(H3N2) Virus Transmission in a Semi‐Isolated Population in a Remote Island in Japan Between 2011 and 2013
Article Snippet: .. One‐Step RT‐PCR Kit (QIAGEN Inc., Valencia, CA, USA) was used for RNA viruses, and GoTaq Flexi DNA Polymerase (Promega, San Luis Obispo, CA, USA) and PCR Nucleotide Mix (Promega, San Luis Obispo, CA, USA) were used for DNA viruses. ..

Article Title: Phylogenetic Study of Local Patterns Influenza A(H3N2) Virus Transmission in a Semi-Isolated Population in a Remote Island in Japan Between 2011 and 2013.
Article Snippet: .. One- Step RT- PCR Kit (QIAGEN Inc., Valencia, CA, USA) was used for RNA viruses, and GoTaq Flexi DNA Polymerase (Promega, San Luis Obispo, CA, USA) and PCR Nucleotide Mix (Promega, San Luis Obispo, CA, USA) were used for DNA viruses. ..

Polymerase Chain Reaction:

Article Title: Phylogenetic Study of Local Patterns Influenza A(H3N2) Virus Transmission in a Semi‐Isolated Population in a Remote Island in Japan Between 2011 and 2013
Article Snippet: .. One‐Step RT‐PCR Kit (QIAGEN Inc., Valencia, CA, USA) was used for RNA viruses, and GoTaq Flexi DNA Polymerase (Promega, San Luis Obispo, CA, USA) and PCR Nucleotide Mix (Promega, San Luis Obispo, CA, USA) were used for DNA viruses. ..

Article Title: Phylogenetic Study of Local Patterns Influenza A(H3N2) Virus Transmission in a Semi-Isolated Population in a Remote Island in Japan Between 2011 and 2013.
Article Snippet: .. One- Step RT- PCR Kit (QIAGEN Inc., Valencia, CA, USA) was used for RNA viruses, and GoTaq Flexi DNA Polymerase (Promega, San Luis Obispo, CA, USA) and PCR Nucleotide Mix (Promega, San Luis Obispo, CA, USA) were used for DNA viruses. ..

Article Title: Bacterial Communities from the Copper Mine of Wettelrode (Germany)
Article Snippet: .. The following components were mixed in the reaction solution (total volume of 50 μL per reaction): 1 μL of DNA isolation eluate, 2 mM MgCl 2 , 200 μM PCR nucleotide mix, 1.25 Units GoTaq G2 Flexi DNA Polymerase, nuclease-free water (all reagents from Promega, Madison, WI, USA), and 1 μmol/l of each primer. ..

Article Title: A long-lived pool of PINK1 imparts a molecular memory of depolarization-induced activity.
Article Snippet: .. RNA isolation and quantitative reverse transcription PCR RNA was extracted using the Qiagen RNA extraction kit (74106). cDNA was then generated using Thermo Fisher Scientific RevertAid H Minus reverse transcription (Thermo Fisher Scientific; 11541515) supplemented with RNasin (Promega; N2611), polymerase chain reaction (PCR) nucleotide mix (Promega; U144B), and oligo (dT) 15 Primer (Promega; C1101). .. Quantitative PCR were performed in triplicate using primers against PINK1 (5’-GGACGCTGTTCCTCGTTA-3′, 5’-ATCTGCGATCACCAGCCA-3′) and ACTB (Actin, 5’-CACCTTCTACAATGAGCTGCGTGTG-3′; 5’-ATAGCACAGCCTGGATAGCAACGTAC-3′), and iTaq Mastermix (Bio- Rad; 172- 5171) in a Bio- Rad CFX Connect real- time system.

Article Title: Increased Myocardial MARK4 Expression in Patients with Heart Failure and Sleep-Disordered Breathing.
Article Snippet: The RNA was isolated using the RNeasy Mini Kit (Qiagen, Hilden, Germany, catalog number 74106). .. The transcription of 1 μg RNA into cDNA was carried out using random primers (Promega Corporation, Madison, WI, USA catalog number C1181), PCR nucleotide mix (Promega, catalog number C1145), Int. ..

Article Title: Improvement of succinate production from methane by combining rational engineering and laboratory evolution in Methylomonas sp. DH-1
Article Snippet: Extracted total RNA was converted to cDNA by reverse transcription, performed in a total 20 μL reactions with GoScriptTM reverse transcriptase (Promega, USA). .. 10 pmol of Random primer (Biorad, USA), 10 mM PCR nucleotide Mix (Promega, USA), 25 mM MgCl 2 (Promega, USA), GoScriptTM 5X Reaction buffer (Promega, USA), and 1 μg of RNA were used in one reaction. .. Samples were annealed at 25 °C for 5 min, extended at 42 °C for 1 h, and incubated at 70 °C for 15 min to inactivate reverse transcriptase using a Q-cycler 96 + (Hain Lifescience, Germany). qPCR analysis was performed with Quantstudio 6 flex (Applied Biosystems, USA) according to the manufacturer’s instructions (KAPA Biosystems, USA).

Article Title: Increased Myocardial MARK4 Expression in Patients with Heart Failure and Sleep-Disordered Breathing
Article Snippet: The RNA was isolated using the RNeasy Mini Kit (Qiagen, Hilden, Germany, catalog number 74106). .. The transcription of 1 μg RNA into cDNA was carried out using random primers (Promega Corporation, Madison, WI, USA catalog number C1181), PCR nucleotide mix (Promega, catalog number C1145), RNasin ® ribonuclease inhibitor (Promega, catalog number N2115), reverse transcriptase (Promega, catalog number M170B), and reverse transcriptase 5x reaction buffer (Promega, catalog number M531A) for 1 h at 37 °C. .. To quantify the MARK4 expression, TaqManTM Fast Advanced Master Mix (Applied Biosystems, Waltham, MA USA, catalog number 4444557) and pre-designed TaqMan ® Gene Expression Assays (Applied Biosystems, catalog number 4331182) for MARK4 (assay ID: Hs00230039_m1) and ACTB (assay ID: Hs01060665_g1) were used.

Article Title: Optimization of a lentivirus-mediated gene therapy targeting HIV-1 RNA to eliminate HIV-1-infected cells
Article Snippet: .. To facilitate subcloning steps, the Xba I and Bcu I restriction sites were introduced to the 5′ end of the EF1α promoter in pEF-GFP (Addgene plasmid #11154) and Nhe I was introduced to the 3′end by mutagenic PCR with 100-pg plasmid template, 1.25 U of GoTaq DNA Polymerase (Promega), 1× GoTaq Buffer (Promega), 200 μM PCR nucleotide mix (Promega), nuclease-free water (Promega), and 200 nM forward and reverse primers described in . ..

DNA Extraction:

Article Title: Bacterial Communities from the Copper Mine of Wettelrode (Germany)
Article Snippet: .. The following components were mixed in the reaction solution (total volume of 50 μL per reaction): 1 μL of DNA isolation eluate, 2 mM MgCl 2 , 200 μM PCR nucleotide mix, 1.25 Units GoTaq G2 Flexi DNA Polymerase, nuclease-free water (all reagents from Promega, Madison, WI, USA), and 1 μmol/l of each primer. ..

Isolation:

Article Title: A long-lived pool of PINK1 imparts a molecular memory of depolarization-induced activity.
Article Snippet: .. RNA isolation and quantitative reverse transcription PCR RNA was extracted using the Qiagen RNA extraction kit (74106). cDNA was then generated using Thermo Fisher Scientific RevertAid H Minus reverse transcription (Thermo Fisher Scientific; 11541515) supplemented with RNasin (Promega; N2611), polymerase chain reaction (PCR) nucleotide mix (Promega; U144B), and oligo (dT) 15 Primer (Promega; C1101). .. Quantitative PCR were performed in triplicate using primers against PINK1 (5’-GGACGCTGTTCCTCGTTA-3′, 5’-ATCTGCGATCACCAGCCA-3′) and ACTB (Actin, 5’-CACCTTCTACAATGAGCTGCGTGTG-3′; 5’-ATAGCACAGCCTGGATAGCAACGTAC-3′), and iTaq Mastermix (Bio- Rad; 172- 5171) in a Bio- Rad CFX Connect real- time system.

Reverse Transcription:

Article Title: A long-lived pool of PINK1 imparts a molecular memory of depolarization-induced activity.
Article Snippet: .. RNA isolation and quantitative reverse transcription PCR RNA was extracted using the Qiagen RNA extraction kit (74106). cDNA was then generated using Thermo Fisher Scientific RevertAid H Minus reverse transcription (Thermo Fisher Scientific; 11541515) supplemented with RNasin (Promega; N2611), polymerase chain reaction (PCR) nucleotide mix (Promega; U144B), and oligo (dT) 15 Primer (Promega; C1101). .. Quantitative PCR were performed in triplicate using primers against PINK1 (5’-GGACGCTGTTCCTCGTTA-3′, 5’-ATCTGCGATCACCAGCCA-3′) and ACTB (Actin, 5’-CACCTTCTACAATGAGCTGCGTGTG-3′; 5’-ATAGCACAGCCTGGATAGCAACGTAC-3′), and iTaq Mastermix (Bio- Rad; 172- 5171) in a Bio- Rad CFX Connect real- time system.

Article Title: Increased Myocardial MARK4 Expression in Patients with Heart Failure and Sleep-Disordered Breathing
Article Snippet: The RNA was isolated using the RNeasy Mini Kit (Qiagen, Hilden, Germany, catalog number 74106). .. The transcription of 1 μg RNA into cDNA was carried out using random primers (Promega Corporation, Madison, WI, USA catalog number C1181), PCR nucleotide mix (Promega, catalog number C1145), RNasin ® ribonuclease inhibitor (Promega, catalog number N2115), reverse transcriptase (Promega, catalog number M170B), and reverse transcriptase 5x reaction buffer (Promega, catalog number M531A) for 1 h at 37 °C. .. To quantify the MARK4 expression, TaqManTM Fast Advanced Master Mix (Applied Biosystems, Waltham, MA USA, catalog number 4444557) and pre-designed TaqMan ® Gene Expression Assays (Applied Biosystems, catalog number 4331182) for MARK4 (assay ID: Hs00230039_m1) and ACTB (assay ID: Hs01060665_g1) were used.

RNA Extraction:

Article Title: A long-lived pool of PINK1 imparts a molecular memory of depolarization-induced activity.
Article Snippet: .. RNA isolation and quantitative reverse transcription PCR RNA was extracted using the Qiagen RNA extraction kit (74106). cDNA was then generated using Thermo Fisher Scientific RevertAid H Minus reverse transcription (Thermo Fisher Scientific; 11541515) supplemented with RNasin (Promega; N2611), polymerase chain reaction (PCR) nucleotide mix (Promega; U144B), and oligo (dT) 15 Primer (Promega; C1101). .. Quantitative PCR were performed in triplicate using primers against PINK1 (5’-GGACGCTGTTCCTCGTTA-3′, 5’-ATCTGCGATCACCAGCCA-3′) and ACTB (Actin, 5’-CACCTTCTACAATGAGCTGCGTGTG-3′; 5’-ATAGCACAGCCTGGATAGCAACGTAC-3′), and iTaq Mastermix (Bio- Rad; 172- 5171) in a Bio- Rad CFX Connect real- time system.

Generated:

Article Title: A long-lived pool of PINK1 imparts a molecular memory of depolarization-induced activity.
Article Snippet: .. RNA isolation and quantitative reverse transcription PCR RNA was extracted using the Qiagen RNA extraction kit (74106). cDNA was then generated using Thermo Fisher Scientific RevertAid H Minus reverse transcription (Thermo Fisher Scientific; 11541515) supplemented with RNasin (Promega; N2611), polymerase chain reaction (PCR) nucleotide mix (Promega; U144B), and oligo (dT) 15 Primer (Promega; C1101). .. Quantitative PCR were performed in triplicate using primers against PINK1 (5’-GGACGCTGTTCCTCGTTA-3′, 5’-ATCTGCGATCACCAGCCA-3′) and ACTB (Actin, 5’-CACCTTCTACAATGAGCTGCGTGTG-3′; 5’-ATAGCACAGCCTGGATAGCAACGTAC-3′), and iTaq Mastermix (Bio- Rad; 172- 5171) in a Bio- Rad CFX Connect real- time system.

Subcloning:

Article Title: Optimization of a lentivirus-mediated gene therapy targeting HIV-1 RNA to eliminate HIV-1-infected cells
Article Snippet: .. To facilitate subcloning steps, the Xba I and Bcu I restriction sites were introduced to the 5′ end of the EF1α promoter in pEF-GFP (Addgene plasmid #11154) and Nhe I was introduced to the 3′end by mutagenic PCR with 100-pg plasmid template, 1.25 U of GoTaq DNA Polymerase (Promega), 1× GoTaq Buffer (Promega), 200 μM PCR nucleotide mix (Promega), nuclease-free water (Promega), and 200 nM forward and reverse primers described in . ..

Plasmid Preparation:

Article Title: Optimization of a lentivirus-mediated gene therapy targeting HIV-1 RNA to eliminate HIV-1-infected cells
Article Snippet: .. To facilitate subcloning steps, the Xba I and Bcu I restriction sites were introduced to the 5′ end of the EF1α promoter in pEF-GFP (Addgene plasmid #11154) and Nhe I was introduced to the 3′end by mutagenic PCR with 100-pg plasmid template, 1.25 U of GoTaq DNA Polymerase (Promega), 1× GoTaq Buffer (Promega), 200 μM PCR nucleotide mix (Promega), nuclease-free water (Promega), and 200 nM forward and reverse primers described in . ..



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