random primer (Promega)
90
Structured Review
Promega
random primer
Random Primer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+goscripttm+buffer/random+primer/pm32781964-107-11-46
Average 90 stars, based on 1 article reviews
Random Primer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+goscripttm+buffer/random+primer/pm32781964-107-11-46
Average 90 stars, based on 1 article reviews
random primer - by Bioz Stars,
2026-09
90/100 stars
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Reverse Transcription:Article Title: Broadening the clinical spectrum: molecular mechanisms and new phenotypes of ANO3-dystonia. Article Snippet: Anoctamin 3 (ANO3) belongs to a family of transmembrane proteins that form phospholipid scramblases and ion channels.. A large number of ANO3 variants were identified as the cause of craniocervical dystonia, but the underlying pathogenic mechanisms remain obscure.. It was suggested that ANO3 variants may dysregulate intracellular Ca signalling, as variants in other Ca regulating proteins like hippocalcin were also identified as a cause of dystonia. Article Title: CXCL16 knockout inhibit asthma airway inflammation by suppressing H2-DM molecular mediated antigen presentation. Article Snippet: Total RNA was obtained from the lungs using TRIzol (Invitrogen, Carlsbad, CA) in accordance with the manufacturer’s directions. .. A total of 2 μg RNA was reverse-transcribed into cDNA with M-MLV and Article Title: Evolutionary dynamics of canine kobuvirus in Vietnam and Thailand reveal the evidence of viral ability to evade host immunity. Article Snippet: The initial step involved the construction of cDNA from the extracted RNA samples, using the Omniscript® Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany). .. For cDNA synthesis, the final volume was 20 μl, comprising 2 μl of 10 × RT buffer, 2 μl of 5 mM dNTP mixture, 1 μl of 10 μM Article Title: Temperature-dependent polar lignification of a seed coat suberin layer promoting dormancy in Arabidopsis thaliana Article Snippet: .. Total RNAs were treated with RQ1 RNase-Free DNase (Promega, Switzerland) and reverse-transcribed using ImpromII reverse transcriptase (Promega) and cDNA Synthesis:Article Title: Evolutionary dynamics of canine kobuvirus in Vietnam and Thailand reveal the evidence of viral ability to evade host immunity. Article Snippet: The initial step involved the construction of cDNA from the extracted RNA samples, using the Omniscript® Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany). .. For cDNA synthesis, the final volume was 20 μl, comprising 2 μl of 10 × RT buffer, 2 μl of 5 mM dNTP mixture, 1 μl of 10 μM Article Title: Decreased mitochondrial transcription factor A and mitochondrial DNA copy number promote cyclin-dependent kinase inhibitor 1A expression and reduce tumorigenic properties of colorectal cancer cells Article Snippet: Total RNA was extracted from cells using the RNeasy ® Plus Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. For complementary DNA (cDNA) synthesis, 1.1 μg RNA were mixed with 3 μl other:Article Title: Effects of pulsed electrical stimulation on α-smooth muscle actin and type I collagen expression in human dermal fibroblasts. Article Snippet: Pulsed electrical stimulation (PES) is known to affect cellular activities.. We previously found PES to human dermal fibroblasts (HFs) promoted platelet-derived growth factor subunit A (PDGFA) gene expression, which enhanced proliferation.. In this study, we investigated PES effects on fibroblast collagen production and differentiation into myofibroblasts. Article Title: Cytosolic DNA sensors activation of human astrocytes inhibits herpes simplex virus through IRF1 induction Article Snippet: RNAs were reverse transcribed using the random primer, dNTPs, M-MLV reverse transcriptase and Incubation:Article Title: Decreased mitochondrial transcription factor A and mitochondrial DNA copy number promote cyclin-dependent kinase inhibitor 1A expression and reduce tumorigenic properties of colorectal cancer cells Article Snippet: Total RNA was extracted from cells using the RNeasy ® Plus Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. .. For complementary DNA (cDNA) synthesis, 1.1 μg RNA were mixed with 3 μl |