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Promega 5x buffer
5x Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+goscripttm+buffer/5%C3%97+buffer/pm30916577-67-63-65
Average 90 stars, based on 1 article reviews
5x buffer - by Bioz Stars, 2026-11
90/100 stars

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Article Title: Continental-scale insights into the sugarbeet diffusion juice microbiomes.
Article Snippet: Each PCR was conducted in a total volume of 25.0 μL, containing 5.0 μL 5× buffer (Promega Corporation, Madison, WI, USA), 0.75 μL 10 mM dNTP mix (Promega Corp., Madison, WI, USA), 0.5 μL of each forward and reverse primer, 0.25 μL Taq polymerase (Promega Corp.), 1.0 μL template DNA, and 14.5 μL nuclease-free molecular grade water (Qiagen, Hilden, Germany).

Article Title: Decreased mitochondrial transcription factor A and mitochondrial DNA copy number promote cyclin-dependent kinase inhibitor 1A expression and reduce tumorigenic properties of colorectal cancer cells
Article Snippet: The mix was immediately transferred on ice for 30 s and 11 μl Master Mix containing 5 μl 5 × buffer (Promega, Walldorf, Germany), 0.8 μl RNAsin plus (Promega, Walldorf, Germany), 1 μl M-MLV Reverse Transcriptase (Promega, Walldorf, Germany) and 4.2 μl of 2.5 mM dNTPs (Thermo Fisher Scientific, Waltham, USA) was added.

Article Title: Acanthocybium solandri (Actinopterygii, Scombriformes, Scombridae) first record from Malta with notes on using its parasites as biological tags
Article Snippet: Polymerase chain reactions (PCRs) were carried out in a 25 μL volume containing 0.5 μL of each primer 10 μM, 3 μL of MgCl2 25 mM (Promega, USA), 5 μL of 5× buffer (Promega), 0.5 μL of DMSO 0.3 mM, 0.5 μL of dNTPs 10 mM (Promega), 0.3 μL of Go-Taq Polymerase (5 U/μL) (Promega), and 2 μL of total DNA template, adding ultrapure water to reach the final volume.

Article Title: Relationships between crayfish population genetic diversity, species richness, and abundance within impounded and unimpounded streams in Alabama, USA
Article Snippet: Polymerase chain reaction (PCR) amplifications were carried out in a final volume of 10 μL, containing the following: 1 μL genomic DNA, 2 μL 5 × buffer (Promega, Maddison WI, USA), 0.8 μL MgCl 2 (25 mM, Promega), 1.6 μL dNTPs (1.25 μM, Promega), 0.5 μL bovine serum albumin (10 mg/μL, New England Biolabs, Ipswich, MA, USA), 3 μL dH 2 O, 0.1 μL Go- Taq DNA polymerase (5 U/μL, Promega), and 1 μL of primer (10 μM).

Article Title: Characteristics of Chrysosporium spp. Pathogens Causing Skin Mycoses in Horses.
Article Snippet: PCR was carried out in a total reaction volume of 25 μL containing 5 μL of 5× buffer (Promega), 1 μL of dNTPs (20 mM), 1.5 μL of MgCl2 (25 mM), 0.25 U (5 U/μL) of Taq DNA polymerase, 2 μL of primers (20 pmol/μL), and 2 μL of genomic DNA (50 ng/μL).

Article Title: Agricultural Soil as a Reservoir of Pseudomonas aeruginosa with Potential Risk to Public Health
Article Snippet: The PCRs were performed using 5× buffer (Promega, USA), 25 mM MgCl 2 (Promega, USA), 10 μM dNTPs (Bioline, USA), 10 μM primers, 5 U/μL Taq DNA polymerase (Promega, USA) and sterile water in a total reaction volume of 25 μL.

Polymerase Chain Reaction:

Article Title: Agricultural Soil as a Reservoir of Pseudomonas aeruginosa with Potential Risk to Public Health
Article Snippet: The specific amplification primer used for PCR assay were rpo D (PsEG30F, 5′-ATYGAAATCGCCAAR CG-3′; PsEG790R, 5′-CGGTTGATKTCCTTGA-3′) for the genus Pseudomonas and ecf X (ECF5, 5′-AAGCGTTCGTCCTGCACAA-3′; ECF2, 5′-TCATCCTTCGCCTCCCTG-3) for the species P. aeruginosa [ , ], with a positive control P. aeruginosa ATCC 27853 ® and ATCC 9027 ® . .. All PCR assays were performed in a 25 μL reaction mixture, containing 5x buffer (Promega, Madison, WI, USA), 25 mM MgCl 2 (Promega, USA), 10 μM dNTPs (Bioline, Camarillo, CA, USA), 10 μM primers, 5 U/μL Taq DNA polymerase (Promega, USA) and sterile water. .. The reaction mixture was incubated in a thermocycler Veriti TM Thermal Cycler (Applied Biosystems TM , Waltham, MA, USA).

Article Title: Agricultural Soil as a Reservoir of Pseudomonas aeruginosa with Potential Risk to Public Health
Article Snippet: .. The PCR reaction mixture was employed in 25 μL reaction volumes containing 5x buffer (Promega, USA), 25 mM MgCl 2 (Promega, USA), 10 μM dNTPs (Bioline, USA), 10 μM primers, 5 U/μL Taq DNA polymerase (Promega, USA), DNA template and sterile water. .. PCR amplification was conducted following these conditions: 95 °C for 1 min, 30 cycles of 95 °C for 45 s, 54 °C for 45 s, 72 °C for 45 s and 72 °C for 7 min. PCR products were visualized in 2.0% agarose gels at 100 V for 45 min. A molecular marker was run concurrently (100 pb Promega, USA).

Sterility:

Article Title: Agricultural Soil as a Reservoir of Pseudomonas aeruginosa with Potential Risk to Public Health
Article Snippet: The specific amplification primer used for PCR assay were rpo D (PsEG30F, 5′-ATYGAAATCGCCAAR CG-3′; PsEG790R, 5′-CGGTTGATKTCCTTGA-3′) for the genus Pseudomonas and ecf X (ECF5, 5′-AAGCGTTCGTCCTGCACAA-3′; ECF2, 5′-TCATCCTTCGCCTCCCTG-3) for the species P. aeruginosa [ , ], with a positive control P. aeruginosa ATCC 27853 ® and ATCC 9027 ® . .. All PCR assays were performed in a 25 μL reaction mixture, containing 5x buffer (Promega, Madison, WI, USA), 25 mM MgCl 2 (Promega, USA), 10 μM dNTPs (Bioline, Camarillo, CA, USA), 10 μM primers, 5 U/μL Taq DNA polymerase (Promega, USA) and sterile water. .. The reaction mixture was incubated in a thermocycler Veriti TM Thermal Cycler (Applied Biosystems TM , Waltham, MA, USA).

Article Title: Agricultural Soil as a Reservoir of Pseudomonas aeruginosa with Potential Risk to Public Health
Article Snippet: .. The PCR reaction mixture was employed in 25 μL reaction volumes containing 5x buffer (Promega, USA), 25 mM MgCl 2 (Promega, USA), 10 μM dNTPs (Bioline, USA), 10 μM primers, 5 U/μL Taq DNA polymerase (Promega, USA), DNA template and sterile water. .. PCR amplification was conducted following these conditions: 95 °C for 1 min, 30 cycles of 95 °C for 45 s, 54 °C for 45 s, 72 °C for 45 s and 72 °C for 7 min. PCR products were visualized in 2.0% agarose gels at 100 V for 45 min. A molecular marker was run concurrently (100 pb Promega, USA).



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